Article
Biochemistry & Molecular Biology
Kelsey Willson, Anthony Atala, James J. Yoo
Summary: Developing appropriate bioinks is a complex task that depends on the mechanical and biochemical requirements of the final construct and the type of printer used. Different types of tissue printers have different requirements for bioinks, with an emphasis on supporting cellular development. Researchers are increasingly using composite bioinks to take advantage of multiple materials in the development process.
Review
Biochemistry & Molecular Biology
Nicolas Germain, Melanie Dhayer, Salim Dekiouk, Philippe Marchetti
Summary: Three-dimensional bioprinting can mimic the tumor microenvironment by producing complex, multi-cellular, and reproducible constructs. This review discusses different 3D bioprinting technologies and their applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Matteo Pitton, Andrea Fiorati, Silvia Buscemi, Lucio Melone, Silvia Fare, Nicola Contessi Negrini
Summary: By developing multicomponent bioinks combining pectin with TEMPO-oxidized cellulose nanofibers (TOCNFs), the research optimized the printability of pectin inks, ensuring stability of the printed hydrogels and viability of cell-laden inks.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Kang Yu, Xinjie Zhang, Yuan Sun, Qing Gao, Jianzhong Fu, Xiujun Cai, Yong He
Summary: Projection-based 3D bioprinting (PBP) is suitable for printing delicate structures, but the requirements for printing details with photocurable bioink are not well understood. This study systematically investigates the printing details using GelMA-based bioink as an example, establishes quantized models for evaluating printing errors, and presents better strategies for bioprinting different structures.
BIOACTIVE MATERIALS
(2022)
Article
Biochemical Research Methods
Shukufe Amukarimi, Zahra Rezvani, Neda Eghtesadi, Masoud Mozafari
Summary: The development of smart biomaterials has led to enhanced medical facilities in this century. One shining example is the rise of four-dimensional bioprinting technology, which can fabricate stimuli-responsive cell-laden structures that can undergo morphological changes in a pre-planned way.
Article
Materials Science, Multidisciplinary
Chen Qin, Jingge Ma, Lei Chen, Hongshi Ma, Hui Zhuang, Meng Zhang, Zhiguang Huan, Jiang Chang, Nan Ma, Chengtie Wu
Summary: This study successfully developed a multicellular system based on a bilayered co-culture scaffold for osteochondral regeneration via a 3D bioprinting strategy. The results show the potential of inducing osteochondral tissue regeneration in vivo through the 3D bioprinted co-culture scaffolds.
Article
Polymer Science
Juo Lee, Sangbae Park, Sungmin Lee, Hae Yong Kweon, You-Young Jo, Jungsil Kim, Jong Hoon Chung, Hoon Seonwoo
Summary: This study addresses the limitations of using natural polymers such as silk fibroin (SF) in 3D bioprinting by developing silk fibroin-based thermosensitive bioinks (SF-TPBs). The gelation time and mechanical strength of SF-TPBs can be precisely modulated by manipulating the glycerol content without the need for cross-linkers. Chemical analysis confirms the presence of β-sheet conformation in SF-TPBs regardless of glycerol concentration. Increased glycerol content improves gelation kinetics and results in rheological properties suitable for 3D printing. Overall, SF-TPBs offer promising prospects for realizing the potential of 3D bioprinting using natural polymers.
Article
Chemistry, Multidisciplinary
Sarah M. Hull, Christopher D. Lindsay, Lucia G. Brunel, Daniel J. Shiwarski, Joshua W. Tashman, Julien G. Roth, David Myung, Adam W. Feinberg, Sarah C. Heilshorn
Summary: UNION bioinks represent a versatile and universal strategy for 3D bioprinting, allowing for the printing of different cell types while maintaining high cell viability and characteristic phenotypic markers. This approach expands the toolkit of customizable materials available for 3D bioprinting.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Review
Pharmacology & Pharmacy
Fatima Garcia-Villen, Sandra Ruiz-Alonso, Markel Lafuente-Merchan, Idoia Gallego, Myriam Sainz-Ramos, Laura Saenz-del-Burgo, Jose Luis Pedraz
Summary: The adaptation and progress of 3D printing technology towards 3D bioprinting for biomedical purposes has allowed for the production of tailor-made constructs and organs, with the potential use of inorganic ingredients such as clays to improve bioinks and biomaterial inks, enhancing their mechanical properties and bioactivities.
Article
Engineering, Biomedical
Fatima Garcia-Villen, Amaia Guembe, Jose M. Rey, Teresa Zuniga, Sandra Ruiz-Alonso, Laura Saenz-del-Burgo, Jesus M. Izco, Jose I. Recalde, Jose Luis Pedraz
Summary: Collagen is a key protein for tissue engineering and 3D bioprinting, but it comes with challenges such as complex extraction processes and batch-to-batch reproducibility issues. This study evaluates the use of new, fibrillar type I collagen as a standardized and reproducible collagen source for 3D printing and bioprinting. The native, type I collagen demonstrated excellent performance during 3D printing and enabled cell inclusion without hindering printability.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Biochemistry & Molecular Biology
Jeong Wook Seo, Gyu Min Kim, Yejin Choi, Jae Min Cha, Hojae Bae
Summary: This study explores optimization of DLP bioprinting by synchronizing the light wavelength and ink absorbance, and introduces photorheological measurements to provide optimized printing conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Polymer Science
Yeong-Jin Choi, Honghyun Park, Dong-Heon Ha, Hui-Suk Yun, Hee-Gyeong Yi, Hyungseok Lee
Summary: This paper provides an in-depth assessment of the recently developed 3D bioprinting technology and bioinks, focusing on the latest achievements and future aspects of using 3D bioprinting for in vitro modeling.
Article
Pharmacology & Pharmacy
Jiawei Wang, Zhengrong Cui, Mohammed Maniruzzaman
Summary: Three-dimensional (3D) bioprinting is a promising technique in tissue engineering, regenerative medicine, and drug delivery. However, optimizing printing resolution and maintaining cell viability during and after bioprinting remain challenges. This review comprehensively analyzes the process parameters affecting bioink printability and cell performance, including bioink properties, printing speed and pressure, nozzle characteristics, and crosslinking parameters. The review also highlights future prospects, such as correlating process parameters with specific cell types, applying statistical analysis and artificial intelligence/machine learning techniques in parameter screening, and optimizing four-dimensional (4D) bioprinting process parameters.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Review
Materials Science, Biomaterials
Ryeim B. Ansaf, Rachel Ziebart, Hemanth Gudapati, Rafaela Mayumi Simoes Torigoe, Stella Victorelli, Joao Passos, Saranya P. Wyles
Summary: As people live longer, age-related skin conditions such as senile purpura, seborrheic keratoses, and skin cancer become more prevalent. However, current pre-clinical animal models are not able to accurately replicate human skin response, necessitating the development of a reliable aged human skin model. This review discusses the use of 3D bioprinting to create an aged skin model, incorporating innate functionality like sensation, vascularization, and regeneration, and provides an overview of current bioprinted skin models and future research directions.
REGENERATIVE BIOMATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Jianhua Zhang, Esther Wehrle, Marina Rubert, Ralph Mueller
Summary: Three-dimensional (3D) bioprinting is a promising approach for tissue engineering, allowing precise placement of cells and biomaterials to mimic complex microstructures of biological tissues. Bioprinted tissues can benefit from exposure to fluid mechanical cues provided by bioreactor systems, aiding in tissue development and function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
