Article
Biochemistry & Molecular Biology
Magda N. Hernandez Vasquez, Maria H. Ulvmar, Alejandra Gonzalez-Loyola, Ioannis Kritikos, Ying Sun, Liqun He, Cornelia Halin, Tatiana Petrova, Taija Makinen
Summary: This study identifies FOXP2 as a new flow-induced transcriptional regulator of collecting lymphatic vessel morphogenesis. Loss of FOXP2 results in enlarged collecting vessels and defective valves, highlighting the unique transcription factor codes in establishing vessel-type-specific endothelial cell identities.
Article
Health Care Sciences & Services
Giuseppe Visconti, Alessandro Bianchi, Marzia Salgarello, Alba Di Leone, Akitatsu Hayashi, Riccardo Masetti, Gianluca Franceschini
Summary: This study presented the experience of lymphatic transfer in 30 patients with extremity lymphedema and 15 patients with post-traumatic lower extremity soft tissue defects. Results indicated that LFT may be a valid treatment option, reducing lymphedema index and improving stability in patients.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Immunology
Rafael S. Czepielewski, Emma C. Erlich, Emily J. Onufer, Shannon Young, Brian T. Saunders, Yong-Hyun Han, Mary Wohltmann, Peter L. Wang, Ki-Wook Kim, Shashi Kumar, Chyi-Song Hsieh, Joshua P. Scallan, Ying Yang, Bernd H. Zinselmeyer, Michael J. Davis, Gwendalyn J. Randolph
Summary: The formation of tertiary lymphoid organs (TLOs) in the mesentery is a feature of Crohn's disease, blocking cellular and molecular outflow from the gut and causing lymph leakage and backflow. TNF neutralization at early stages can restore lymph transport, but developed chronic TLOs resist regression and restoration of flow after TNF neutralization. TNF stimulation of lymphatic endothelial cells disrupts immune cell transport and valve-associated gene induction, contributing to Crohn's disease pathology.
Article
Oncology
Jun Ye, Yue Gao, Ming Ji, Yanfang Yang, Zhaohui Wang, Baolian Wang, Jing Jin, Ling Li, Hongliang Wang, Xiaoyan Xu, Hengfeng Liao, Chunfang Lian, Yaqi Xu, Renjie Li, Tong Sun, Lili Gao, Yan Li, Xiaoguang Chen, Yuling Liu
Summary: This study developed a self-microemulsifying drug delivery system carrying chlorogenic acid, successfully delivering immunomodulators to mesenteric lymph nodes efficiently. Through promoting immune cell activity and inhibiting immunosuppressive factors, it achieved significant antitumor effects in inhibiting tumor growth.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Physiology
Scott D. Zawieja, Grace A. Pea, Sarah E. Broyhill, Advaya Patro, Karen H. Bromert, Min Li, Charles E. Norton, Jorge A. Castorena-Gonzalez, Edward J. Hancock, Christopher D. Bertram, Michael J. Davis
Summary: The study reveals the critical role of IP3R1 in regulating pressure-dependent chronotropy and contractile regulation in lymphatic vessels. IP3R1-mediated calcium release is identified as the primary calcium source driving Anoctamin1 activation during diastole, controlling diastolic depolarization. These findings contribute to a better understanding of the regulatory mechanisms in lymphatic vessels.
JOURNAL OF GENERAL PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jia Guo, Zeyu Xu, Rachel C. Gunderson, Baohui Xu, Sara A. Michie
Summary: In this study, the researchers found that ICAM-1 is expressed on the sinus endothelia of lymph nodes, but not on the lymphatic vessels of peripheral tissues. Memory CD4(+) T cells migrated to lymph nodes from peripheral tissues more efficiently than from blood vessels. ICAM-1 deficiency significantly inhibited the migration of lymphocytes from peripheral tissues into lymph nodes, and the absence of ICAM-1 or LFA-1 reduced the number of memory T cells in lymph nodes.
Article
Hematology
Michiko Jo, Andrea N. Trujillo, Naotoshi Shibahara, Jerome W. Breslin
Summary: This study aimed to evaluate the effects of Goreisan and its five components on lymphatic pump function. The results showed that Alismatis Rhizoma and Polyporus significantly reduced the contraction frequency and fractional pump flow of the lymphatic pump. Additionally, oral administration of Goreisan and Alismatis Rhizoma increased the expression of VEGFR-3 in mesenteric collecting lymphatics.
Article
Hematology
Kazuki Horiuchi, Masaaki Higashiyama, Chie Kurihara, Kouji Matsumura, Rina Tanemoto, Suguru Ito, Akinori Mizoguchi, Shin Nishii, Akinori Wada, Kenichi Inaba, Nao Sugihara, Yoshinori Hanawa, Naoki Shibuya, Yoshikiyo Okada, Chikako Watanabe, Shunsuke Komoto, Kengo Tomita, Ryota Hokari
Summary: In this study, it was found that intestinal innate lymphoid cells (ILCs) migrate through mesenteric lymphatic vessels (MLVs) and exhibit different characteristics under physiological and pathological conditions.
Article
Biochemical Research Methods
Guillermo Arroyo-Ataz, Dennis Jones
Summary: This article presents methods and procedures for studying smooth muscle cells in lymphatic and blood vessels using in vivo and in vitro systems.
Article
Physiology
Jens Majgaard, Frederik G. Skov, Sukhan Kim, Vibeke Elisabeth Hjortdal, Donna M. B. Boedtkjer
Summary: This study investigated the potential role of HCN channels in controlling contractile function in human collecting lymphatic vessels. The results showed that although HCN channel proteins were expressed in these vessels, HCN inhibitors did not lower contraction frequency in ex vivo experiments. Instead, they induced positive chronotropic and negative inotropic effects.
PHYSIOLOGICAL REPORTS
(2022)
Review
Medicine, Research & Experimental
Elisabetta Weber, Margherita Agliano, Eugenio Bertelli, Guido Gabriele, Paolo Gennaro, Virginia Barone
Summary: Secondary lymphedema is a common side effect of oncological treatments, affecting the daily lives of patients and the healthcare system. Treatment involves surgical techniques to connect lymphatic vessels and veins, but successful results depend on the functional structure of the vessel. This article reviews the structure of lymphatic collecting vessels in health and in lymphedema, focusing on histopathological changes that affect the success of lymphatic-venular anastomosis.
LYMPHATIC RESEARCH AND BIOLOGY
(2022)
Article
Oncology
Shadi Younes, Andreas M. Kaufmann, Norman Haefner, Katrin Beer, Lars Jansen, Juliane Sanft, Gita Mall, Susan Koops, Matthias Duerst, Achim Schneider
Summary: The study analyzed the presence of tumor cells in lymphatic vessels in patients with cervical cancer and discussed the potential pathological significance of afferent lymphatic vessels to sentinel lymph nodes.
Article
Biochemistry & Molecular Biology
Thaise Boeing, Silvia Speca, Priscila de Souza, Anthony Martin Mena, Benjamin Bertin, Pierre Desreumax, Luisa Mota da Silva, Sergio Faloni de Andrade, Laurent Dubuqoy
Summary: Luteolin can reduce inflammation and oxidative stress induced by irinotecan in Caco-2 cells by upregulating the expression of PPAR gamma, HO-1, and SOD genes, and decreasing the expression of IL-1 beta and iNOS. However, when PPAR gamma expression is downregulated, luteolin loses this effect.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Gastroenterology & Hepatology
Mohamed Bou Saleh, Alexandre Louvet, Line Carolle Ntandja-Wandji, Emmanuel Boleslawski, Viviane Gnemmi, Guillaume Lassailly, Stephanie Truant, Francois Maggiotto, Massih Ningarhari, Florent Artru, Emilie Anglo, Pau Sancho-Bru, Anne Corlu, Josepmaria Argemi, Julie Dubois-Chevalier, Sebastien Dharancy, Jerome Eeckhoute, Ramon Bataller, Philippe Mathurin, Laurent Dubuquoy
Summary: The study evaluated the Hippo/Yes-associated protein (YAP) pathway in patients with alcoholic hepatitis, finding that aberrant YAP activation plays a crucial role in hepatocyte transdifferentiation, loss of identity, and impaired regeneration. Inhibition of YAP may offer a promising therapeutic strategy for alcoholic hepatitis.
JOURNAL OF HEPATOLOGY
(2021)
Article
Environmental Sciences
Eva Guilloteau, Madjid Djouina, Segolene Caboche, Christophe Waxin, Karine Deboudt, Delphine Beury, David Hot, Muriel Pichavant, Laurent Dubuquoy, David Launay, Cecile Vignal, Marie Choel, Mathilde Body-Malapel
Summary: The development of nanotechnologies has led to an increase in engineered nanoparticles (EN) in the environment, including the atmosphere. This study assessed the impact of four prevalent atmospheric EN (silver, titanium dioxide, titanium, and silicon dioxide) on intestinal inflammation and microbiota. The results suggest that these EN can disrupt intestinal homeostasis by modulating cytokine expression and altering the inflammatory response and microbiota composition.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Neurosciences
Irene Matre Thowsen, Tine Karlsen, Elham Nikpey, Hanne Haslene-Hox, Trude Skogstrand, Gwendalyn J. Randolph, Bernd H. Zinselmeyer, Olav Tenstad, Helge Wiig
Summary: Recent studies have shown that the skin acts as a major reservoir for sodium ions by regulating the content of glycosaminoglycans and osmotic gradients. This study investigated the presence of electrolyte gradients in the skin and the storage of sodium ions from a fluid balance perspective. The results revealed a gradient of sodium ions from the epidermis to the dermis, with a higher concentration in the epidermis. This hydration-dependent concentration of sodium ions in the interstitial fluid contributes to the skin barrier and limits water loss. Additionally, intracellular storage of sodium ions in muscle and myocardium may have electromechanical consequences in salt stress.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Article
Toxicology
Eva Guilloteau, Patrice Coll, Zhuyi Lu, Madjid Djouina, Mathieu Cazaunau, Christophe Waxin, Antonin Berge, Segolene Caboche, Aline Gratien, Elie Al Marj, David Hot, Laurent Dubuquoy, David Launay, Cecile Vignal, Sophie Lanone, Mathilde Body-Malapel
Summary: This study suggests that prenatal exposure to air pollution can lead to sex-specific alterations in intestinal development and microbiota in mice.
PARTICLE AND FIBRE TOXICOLOGY
(2022)
Article
Multidisciplinary Sciences
Francois Fasquelle, Laurent Dubuquoy, Didier Betbeder
Summary: The nasal route of immunization is an alternative to injections and is considered more effective at inducing immune protection. This study found that the delivery of antigens using starch nanoparticles can enhance their immunogenicity without the use of immunomodulators.
Article
Environmental Sciences
Madjid Djouina, Christophe Waxin, Frederic Lepretre, Meryem Tardivel, Olivier Tillement, Francis Vasseur, Martin Figeac, Antonino Bongiovanni, Sheherazade Sebda, Pierre Desreumaux, David Launay, Laurent Dubuquoy, Mathilde Body-Malapel, Cecile Vignal
Summary: This study found that aluminum exposure induced cytokine secretion in the colon of CD patients but not healthy individuals. Aluminum internalization in Caco-2 cells was correlated with inflammatory status. Analysis of genetic polymorphisms and transporter expression in human colon showed that decreased activity of ABCB1 and SLC26A3 transporters was involved in aluminum-induced inflammation.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Review
Oncology
Cyril Sobolewski, Laurent Dubuquoy, Noemie Legrand
Summary: AU-Rich Element Binding Proteins (AUBPs) are important post-transcriptional regulators that interact with miRNAs to form a complex regulatory network. MiRNAs are well-studied post-transcriptional regulators of gene expression, and their altered expression is closely associated with various diseases and cancers. However, the influence of other factors, such as long-noncoding RNAs and RNA-Binding-Proteins, adds complexity to this regulatory mechanism.
Article
Biology
Giovanni de Nola, Berenice Leclercq, Alexandra Mougel, Solenne Taront, Claire Simonneau, Federico Forneris, Eric Adriaenssens, Herve Drobecq, Luisa Iamele, Laurent Dubuquoy, Oleg Melnyk, Ermanno Gherardi, Hugo de Jonge, Jerome Vicogne
Summary: Hepatocyte growth factor/scatter factor (HGF/SF) and its receptor MET play important roles in embryogenesis and regeneration of epithelial organs. However, the limited stability and diffusion of native HGF/SF have led to a search for HGF/SF mimics. This study introduces K1K1, a novel MET agonist that is highly stable and displays superior biological activity compared to native HGF/SF, suggesting its potential applications in the treatment of acute and chronic liver diseases.
LIFE SCIENCE ALLIANCE
(2022)
Article
Environmental Sciences
Madjid Djouina, Cecile Vignal, Alexandre Dehaut, Segolene Caboche, Nell Hirt, Christophe Waxin, Charlotte Himber, Delphine Beury, David Hot, Laurent Dubuquoy, David Launay, Guillaume Duflos, Mathilde Body-Malapel
Summary: The aim of this study was to investigate the effects of intestinal exposure to polyethylene (PE) microplastics on gut homeostasis. Mice were orally exposed to PE microbeads of two different sizes, and it was found that ingestion of PE microbeads led to intestinal disturbances and significant alterations of crucial intestinal markers. This highlights the need for further research on the health impact of microplastic exposure in humans.
ENVIRONMENTAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Marie Bobowski-Gerard, Clemence Boulet, Francesco P. Zummo, Julie Dubois-Chevalier, Celine Gheeraert, Mohamed Bou Saleh, Jean-Marc Strub, Amaury Farce, Maheul Ploton, Loic Guille, Jimmy Vandel, Antonino Bongiovanni, Ninon Very, Eloise Woitrain, Audrey Deprince, Fanny Lalloyer, Eric Bauge, Lise Ferri, Line-Carolle Ntandja-Wandji, Alexia K. Cotte, Corinne Grangette, Emmanuelle Vallez, Sarah Cianferani, Violeta Raverdy, Robert Caiazzo, Viviane Gnemmi, Emmanuelle Leteurtre, Benoit Pourcet, Rejane Paumelle, Kim Ravnskjaer, Guillaume Lassailly, Joel T. Haas, Philippe Mathurin, Francois Pattou, Laurent Dubuquoy, Bart Staels, Philippe Lefebvre, Jerome Eeckhoute
Summary: The transcription factor Basonuclin 2 (BNC2) is found to play a key role in driving myofibroblast activation in liver fibrosis. Its expression is induced in fibrotic livers and it integrates pro-fibrotic stimuli to induce matrisome gene expression. BNC2 deficiency leads to reduced collagen deposition in the liver. Furthermore, the study identifies a potential drug target, CC-885, as a BNC2 inhibitor.
NATURE COMMUNICATIONS
(2022)
Article
Pharmacology & Pharmacy
S. Moutaharrik, A. Maroni, C. Neut, C. Dubuquoy, L. Dubuquoy, A. Foppoli, M. Cerea, L. Palugan, F. Siepmann, J. Siepmann, A. Gazzaniga
Summary: A novel drug delivery system based on multiple drug release triggers was developed and evaluated for oral colon delivery, with a specific focus on inflammatory bowel disease (IBD) therapy. The system demonstrated pulsatile drug release in different release media and faster drug release in simulated colonic fluid with fecal bacteria from IBD patients. Promising results were observed in a preliminary rat study, indicating potential therapeutic effects on colitis progression and microbiota alteration.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Norhane Salah, Laurent Dubuquoy, Rodolphe Carpentier, Didier Betbeder
Summary: This study investigated the use of crosslinked starch nanocarrier (NPL) on the intracellular delivery and anti-inflammatory efficiency of curcumin in inflammatory bowel disease (IBD). The results showed that NPL enhanced the intracellular delivery of curcumin in inflammatory cells and decreased the secretion of pro-inflammatory cytokines while increasing the secretion of anti-inflammatory cytokines. Additionally, NPL/Cur exhibited better paracellular transport across the epithelium and showed potential as a therapeutic alternative for IBD management.
INTERNATIONAL JOURNAL OF PHARMACEUTICS-X
(2022)
Article
Biochemistry & Molecular Biology
Julie Dubois-Chevalier, Celine Gheeraert, Alexandre Berthier, Clemence Boulet, Vanessa Dubois, Loic Guille, Marie Fourcot, Guillemette Marot, Karine Gauthier, Laurent Dubuquoy, Bart Staels, Philippe Lefebvre, Jerome Eeckhoute
Summary: Cell identity is controlled by a core transcriptional regulatory circuitry (CoRC) consisting of cell-specific transcription factors (TFs). However, our study reveals that the transcriptional regulatory network regulating hepatocyte identity is more complex than initially thought. We identified a set of TFs, which we call hepatocyte identity (Hep-ID)(CONNECT) TFs, that not only control hepatocyte identity effector genes, but also interact with TFs of the CoRC. Furthermore, we found that Hep-IDCONNECT TFs play a role in fine-tuning CoRC TF expression and can reset CoRC TF expression in dedifferentiated hepatocytes.
Article
Chemistry, Medicinal
Morgane Rivoal, Laurent Dubuquoy, Regis Millet, Natascha Leleu-Chavain
Summary: RIPK2 is an essential downstream signaling molecule that activates various immune receptors, playing a crucial role in the release of pro-inflammatory cytokines. It has emerged as a potential therapeutic target for inflammatory diseases and cancer. This Perspective provides an overview of RIPK2 function, its role in diseases, available inhibitors, therapeutic strategies, and current challenges and outlook.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)