In vivo Protein Evolution, Next Generation Protein Engineering Strategy: from Random Approach to Target-specific Approach

Accurate prediction of protein contact maps by coupling residual two-dimensional bidirectional long short-term memory with convolutional neural networks

(2018) Jack Hanson et al.   BIOINFORMATICS

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window

(2018) Shakked O. Halperin et al.   NATURE

A synthetic microbial biosensor for high-throughput screening of lactam biocatalysts

(2018) Soo-Jin Yeom et al.   Nature Communications

CRISPR EnAbled Trackable genome Engineering for isopropanol production in Escherichia coli

(2017) Liya Liang et al.   METABOLIC ENGINEERING

Phage-assisted continuous evolution of proteases with altered substrate specificity

(2017) Michael S. Packer et al.   Nature Communications

Continuous evolution of Bacillus thuringiensis toxins overcomes insect resistance

(2016) Ahmed H. Badran et al.   NATURE

The coming of age of de novo protein design

(2016) Po-Ssu Huang et al.   NATURE

Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering

(2016) Andrew D Garst et al.   NATURE BIOTECHNOLOGY

Methods for the directed evolution of proteins

(2015) Michael S. Packer et al.   NATURE REVIEWS GENETICS

Identification and characterization of essential genes in the human genome

(2015) T. Wang et al.   SCIENCE

Development of potent in vivo mutagenesis plasmids with broad mutational spectra

(2015) Ahmed H. Badran et al.   Nature Communications

Protein engineering of α2,3/2,6-sialyltransferase to improve the yield and productivity of in vitro sialyllactose synthesis

(2013) Yun Hee Choi et al.   GLYCOBIOLOGY

Engineering of TEV protease variants by yeast ER sequestration screening (YESS) of combinatorial libraries

(2013) L. Yi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Direct Molecular Evolution of Detergent-Stable G Protein-Coupled Receptors Using Polymer Encapsulated Cells

(2012) Daniel J. Scott et al.   JOURNAL OF MOLECULAR BIOLOGY

Genome-scale promoter engineering by coselection MAGE

(2012) Harris H Wang et al.   NATURE METHODS

A system for the continuous directed evolution of biomolecules

(2011) Kevin M. Esvelt et al.   NATURE

Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement

(2011) Farren J. Isaacs et al.   SCIENCE

Novel Activity of UDP-Galactose-4-Epimerase for Free Monosaccharide and Activity Improvement by Active Site-Saturation Mutagenesis

(2010) Hye-Jung Kim et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Technologies of directed protein evolution in vivo

(2010) Artem Blagodatski et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Fine-Tuning of Catalytic Properties of Catechol 1,2-Dioxygenase by Active Site Tailoring

(2009) Raffaella Caglio et al.   CHEMBIOCHEM

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Creating lactose phosphorylase enzymes by directed evolution of cellobiose phosphorylase

(2009) M. R.M. De Groeve et al.   PROTEIN ENGINEERING DESIGN & SELECTION

In Vivo Evolution of Butane Oxidation by Terminal Alkane Hydroxylases AlkB and CYP153A6

(2008) D. J. Koch et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY