Review
Materials Science, Multidisciplinary
Zi-Bo Chen, Hui-Hui Jin, Zhu-Gen Yang, Da-Ping He
Summary: Metal-based nanomaterials have a wide range of applications, and this review focuses on their use in electrochemical impedance spectroscopy (EIS) biosensors for medical healthcare, environmental monitoring, and food safety. The review discusses the importance of bioreceptors and metal nanomaterials in the design and function of these biosensors, and provides insights for future development.
Review
Chemistry, Physical
Veronika Pinkova Gajdosova, Lenka Lorencova, Anna Blsakova, Peter Kasak, Tomas Bertok, Jan Tkac
Summary: The review discusses the challenges and solutions for the successful design of impedimetric biosensors for highly sensitive affinity detection of proteins, including the effects of gold etching, ionic strength, interface properties, and analyte:bioreceptor ratio. Additionally, it touches on other factors like conductive interfaces, small bioreceptors, and strategies for improved selectivity and sensitivity.
CURRENT OPINION IN ELECTROCHEMISTRY
(2021)
Article
Engineering, Electrical & Electronic
Sammy A. Perdomo, Viviana Ortega, Andres Jaramillo-Botero, Nelson Mancilla, Jose Hernando Mosquera-DeLaCruz, Drochss Pettry Valencia, Mauricio Quimbaya, Juan David Contreras, Gabriel Esteban Velez, Oscar A. Loaiza, Adriana Gomez, Jhonattan de la Roche
Summary: One critical step to solving the SARS-CoV-2 pandemic is to stop its spread by detecting and isolating asymptomatic individuals infected with the virus. Unfortunately, current rapid testing tools lack the ability for early detection. SenSARS is an accurate, portable, and low-cost medical device that offers real-time diagnostics and viral load tracking capabilities.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2021)
Article
Chemistry, Analytical
Yuqian Zhang, Yuguang Liu
Summary: A comprehensive system combining electrochemical impedance spectroscopy and digital microfluidics has been introduced to easily and sensitively evaluate a patient's immune function. The unique droplet manipulation feature of the microfluidic platform allows for dynamic cell capture assay, improving detection signal by 2.4-fold, demonstrating high sensitivity.
Article
Chemistry, Analytical
Hend S. Magar, Rabeay Y. A. Hassan, Ashok Mulchandani
Summary: Electrochemical impedance spectroscopy (EIS) is a powerful technique for analyzing interfacial properties related to bio-recognition events at the electrode surface, with applications in biomedical diagnosis and environmental monitoring. The use of nanomaterials enhances the analytical features of impedimetric biosensors, promoting faster electron transfer and improving sensor reliability and accuracy.
Article
Chemistry, Analytical
Araz Norouz Dizaji, Zahraa Ali, Hamed Ghorbanpoor, Yasin Ozturk, Iremnur Akcakoca, Huseyin Avci, Fatma Dogan Guzel
Summary: The study developed an antibiotic-based biosensor platform for specific detection of gram-positive bacteria. The platform demonstrated selectivity and sensitivity towards vancomycin-susceptible bacteria, showing potential for different applications in the future.
Review
Chemistry, Multidisciplinary
Mingji Wei, Rongbiao Zhang, Fei Zhang, Ning Yang, Yecheng Zhang, Guoxiao Li
Summary: The review discusses issues and suggestions regarding the design of electrode systems for cell adhesion-based and nonadhesion-based measurement, as well as the advantages and disadvantages of equivalent circuit models for different requirements. Factors influencing electric impedance spectroscopy are quantitatively analyzed to contribute to a better understanding of the equivalent models.
Article
Biochemistry & Molecular Biology
Rhea Patel, Madhuri Vinchurkar, Aatha Mohin Shaikh, Rajul Patkar, Andrea Adami, Flavio Giacomozzi, Raman Ramesh, Bidhan Pramanick, Leandro Lorenzelli, Maryam Shojaei Baghini
Summary: This article presents the development of a label-free DNA-based biosensor for detecting Ralstonia solanacearum using non-faradaic electrochemical impedance spectroscopy. The sensor demonstrates high sensitivity, specificity, and electrochemical stability. The study further investigates the specificity of the sensor by testing different strains of R. solanacearum isolated from various host plants in Goa, India.
BIOELECTROCHEMISTRY
(2023)
Article
Multidisciplinary Sciences
S. Reza Mahmoodi, Pengfei Xie, Daniel P. Zachs, Erik J. Peterson, Rachel S. Graham, Claire R. W. Kaiser, Hubert H. Lim, Mark G. Allen, Mehdi Javanmard
Summary: A non-faradaic label-free cortisol sensing platform was developed using a nanowell array design, allowing for rapid and low volume sensing. The sensor, built on a glass substrate, showed a good response to cortisol concentrations and compared well with results from standard ELISA. Nanowell array sensors have potential to be a promising platform for point-of-care testing.
Article
Cardiac & Cardiovascular Systems
Julia Boettner, Sarah Werner, Lukas Feistner, Tina Fischer-Schaepmann, Katherina Neussl, Michael A. Borger, Holger Thiele, Petra Buettner, Florian Schlotter
Summary: Fibro-calcific aortic valve disease is a prevalent condition that is associated with high mortality. Using real-time impedance spectroscopy, the ECM remodeling stimulated by procalcifying or profibrotic medium in valvular interstitial cells was monitored. The study revealed sex-specific differences and highlighted the potential of impedance spectroscopy as a screening tool for studying the disease.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Editorial Material
Electrochemistry
Amin Moradpour, Manuel Kasper, Ferry Kienberger
Summary: Electrochemical impedance spectroscopy (EIS) is extensively used in battery cell testing for industrial production and R&D labs. This study focuses on the application of EIS calibration and uncertainty analysis in cell classification. By investigating five scenarios, the qualitative impact of calibration and uncertainty on classification is discussed. An experimental demonstration was conducted on a cylindrical cell using two different quality mechanical fixtures, and the cell was characterized in terms of random errors and calibrated impedances. The results show the impact of uncertainty and impedance calibration on cell classification, and a confidence level is established for the classification results based on the uncertainty and corresponding error bounds. Quantitative uncertainty bounds are presented for the full EIS frequency spectrum ranging from 150 mHz to 5 kHz.
BATTERIES & SUPERCAPS
(2023)
Article
Electrochemistry
Amin Moradpour, Manuel Kasper, Ferry Kienberger
Summary: This article discusses the application of EIS calibration and uncertainty analysis in cell classification. The impact of calibration and uncertainty on classification is qualitatively discussed through five scenarios. An experimental demonstration is conducted on a cylindrical cell using two mechanical fixtures of different qualities to measure random errors and calibrated impedances. The study shows the effects of uncertainty and impedance calibration on cell classification, and a confidence level is established for the classification results based on uncertainty and corresponding error bounds. Quantitative uncertainty bounds are presented for the full EIS frequency spectrum ranging from 150 mHz to 5 kHz.
BATTERIES & SUPERCAPS
(2023)
Article
Chemistry, Analytical
HuanWen Han, Kazuyuki Nobusawa, Ichiro Yamashita
Summary: The DNA intercalator [Ru(bpy)(2)DPPZ](2+) was found to significantly increase charge transfer in electrochemical impedance spectroscopy (EIS), making it a promising candidate for applications in dsDNA detection or PCR monitoring. Future developments may lead to EIS sensors that do not require specific electrode modifications.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Sikander Ameer, Hussam Ibrahim, Muhammad Usama Yaseen, Fnu Kulsoom, Stefano Cinti, Mazhar Sher
Summary: Biofilms are complex communities of microorganisms that can form on various surfaces, and their presence can lead to infections, reduced efficiency, biofouling, and environmental damage. Electrochemical impedance sensing has emerged as a promising approach for real-time, non-destructive, and rapid monitoring of biofilms, with high sensitivity, versatility, and low cost.
Article
Chemistry, Analytical
Alessia Calabrese, Alessandro Capo, Angela Capaccio, Elettra Agovino, Antonio Varriale, Michelangelo Pascale, Sabato D'Auria, Maria Staiano
Summary: Food allergies are immune system responses to specific foods, with common allergens including milk, eggs, seafood, soy, peanuts, tree nuts, wheat, and their derivative products. Chicken egg ovalbumin (OVA) is a common allergen used in wine clarification, and traces of OVA in wine can cause allergic reactions. To address this issue, a non-Faradaic impedimetric immunosensor was developed to detect OVA in wine. The immunosensor, which immobilized polyclonal antibodies anti-OVA on a modified gold surface, could detect OVA in diluted white wine with a sensitivity of 0.20 μg/mL, meeting the limits set for allergen quantification in wine.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)