Article
Biophysics
Felix Hempel, Jessica Ka Yan Law, Thanh Chien Nguyen, Ruben Lanche, Anna Susloparova, Xuan Thang Vu, Sven Ingebrandt
Summary: The study investigated single-cell adhesion on organic electrochemical transistors using an impedimetric readout method, demonstrating single-cell resolution and potential future applications.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Zheyuan Zhang, Xichen Yuan, Huijie Guo, Peng Shang
Summary: Detection sensitivity is crucial in ECIS sensor application, and electrode configuration directly affects sensitivity. This study fabricated ECIS sensor arrays with various electrode configurations to monitor living MC3T3-E1 cells. The results showed that smaller and fewer electrodes with a diameter larger than 25 μm had higher impedance values and generated more impedance shift to cell status change. However, when the electrode diameter was smaller, there was no clear relationship between impedance and membrane capacitance shift to cell status change and electrode geometry. The proposed sensor chip provides a basis for selecting electrode configuration of monopolar electrodes and enables sustained and stable detection of cellular impedance.
Review
Engineering, Electrical & Electronic
Ioana Voiculescu, Fang Li, Anis Nurashikin Nordin
Summary: The ECIS technique is a powerful and effective tool for real-time monitoring of cell behavior, with applications ranging from impedance of cancer cells and toxicity studies to analysis of beating cardiomyocytes. It offers advantages over conventional assays and provides opportunities for innovative research in the field of mammalian cell properties and physiological functions.
IEEE SENSORS JOURNAL
(2021)
Article
Chemistry, Analytical
Laverne Diana Robilliard, Jane Yu, Sung-Min Jun, Akshata Anchan, Graeme Finlay, Catherine E. Angel, Euan Scott Graham
Summary: Glioblastoma is known as the most aggressive and lethal form of brain cancer, comprising differentiated cells and stem cells. ECIS shows reliable measurement of adhesion for differentiated glioblastoma cells and their migratory behavior post-ablation, but is less capable for weakly adherent stem cell counterparts.
Article
Biochemical Research Methods
Yitao Liang, Lin Ji, Tingting Tu, Shanshan Zhang, Bo Liang, Xuesong Ye
Summary: This study developed an on-site platform to monitor the invasion process of cancer cells, optimized parameters to demonstrate experimental results, indicating that changes in impedance magnitude reflect interactions between cells and the extracellular matrix. Further use of this platform could contribute to research on cell metastasis.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Chemistry, Analytical
Chen Zhou, Sebastian Bette, Aaron Babendreyer, Christina Hoffmann, Sven Gerlach, Tom Kremers, Andreas Ludwig, Bernd Hoffmann, Rudolf Merkel, Stefan Uhlig, Uwe Schnakenberg
Summary: The development of a cell-stretching platform utilizing stretchable microelectrodes and an ultra-elastic substrate allows for up to 35% cyclic stretch. The novel interlaced meander design ensures reliable electrical contacting of the microelectrodes under stretch, enabling label-free, real-time monitoring of cell monolayers. Proof-of-concept experiments show that the system can discern impedance changes in different epithelial cell types, providing a potential tool for continuous monitoring of cell monolayers under strain for various applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Materials Science, Multidisciplinary
S. Nithiananth, K. Silambarasan, T. Logu, S. Harish, R. Ramesh, C. Muthamizhchelvan, M. Shimomura, J. Archana, M. Navaneethan
Summary: The study focused on preparing low-cost counter electrodes for DSSCs, utilizing Cu- (In, Co, Ni, Mn)-Se-2 material, with CuNiSe2 exhibiting the lowest charge transfer resistance and showing superior performance in terms of photovoltaic efficiency.
Article
Chemistry, Multidisciplinary
Ning Yang, Wenhao Hui, Sizhe Dong, Xuming Zhang, Liyang Shao, Yanwei Jia, Pui-In Mak, Rui Paulo da Silva Martins
Summary: A method for joint assessment of cell viability and vitality based on ECIS technology and environmental temperature control was proposed, providing accurate evaluation of cell health status. Compared to traditional methods, this approach is simpler, faster, and less dependent on sample conditions.
Article
Chemistry, Analytical
Fuentes-Velez Susana, Fagoonee Sharmila, Sanginario Alessandro, Gallo Valentina, Riganti Chiara, Pizzi Marco, Altruda Fiorella, Demarchi Danilo
Summary: Interest in impedance-based cellular assays is growing due to their multiple advantages, and our study demonstrates the potential of ECIS in cancer treatment decisions and early detection of chemoresistance. The analysis of impedance-based curves distinguishes between chemo-sensitive and chemo-resistant cells, and promises the study of time-dependent factors related to resistance acquisition in cells. The continuous monitoring and highthroughput early detection of drug resistance using the ECIS device presents new possibilities for cancer treatment and drug screening.
Article
Biochemistry & Molecular Biology
Monika Prendecka-Wrobel, Dominika Pigon-Zajac, Magdalena Jaszek, Anna Matuszewska, Dawid Stefaniuk, Grzegorz Opielak, Katarzyna Piotrowska, Mansur Rahnama-Hezavah, Teresa Malecka-Massalska
Summary: This study investigated the pharmacokinetics of an active substance obtained from the medicinal fungus Cerrena unicolor at different concentrations. The results showed that the substance had no significant effects on normal cells, but exhibited inhibitory effects on colon cancer cells.
Article
Cell Biology
Ineke D. C. Jansen, Thijs van Velzen, Teun J. de Vries, Robert Szulcek, Jack J. W. A. van Loon
Summary: In this study, an impedimetric bioassay was introduced for real-time monitoring of osteoclast resorption. The method involves culturing osteoclasts on a calcium phosphate substrate with electrodes and applying different chemical stimuli to measure their activity continuously. The results showed that inflammatory factors and bisphosphonates significantly affected osteoclastic activity. This method is of great importance for studying osteoclast function and diseases, as well as for high-throughput drug testing.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Analytical
Anna Ronja Dorothea Binder, Andrej-Nikolai Spiess, Michael W. Pfaffl
Summary: Measurement of cell surface coverage using electric cell-substrate impedance sensing (ECIS) is a common technique for assessing cell growth behavior. A large toolset of R scripts was developed to transform and quantify ECIS growth curves in different experimental settings, allowing extraction of quantitative data for analysis. The scripts were tested in real-life cell culture experiments and calibration setups, providing comparability and reliable quantification.
Article
Chemistry, Analytical
Yuqian Zhang, Yuguang Liu
Summary: A comprehensive system combining electrochemical impedance spectroscopy and digital microfluidics has been introduced to easily and sensitively evaluate a patient's immune function. The unique droplet manipulation feature of the microfluidic platform allows for dynamic cell capture assay, improving detection signal by 2.4-fold, demonstrating high sensitivity.
Article
Nanoscience & Nanotechnology
Yu-Sheng Hsiao, Edgar Daniel Quinones, Shih-Chieh Yen, Jiashing Yu, Ji-Tseng Fang, Peilin Chen, Ruey-Shin Juang
Summary: Electric cell-substrate impedance sensing (ECIS) is an innovative approach for the label-free and real-time detection of cell morphology, growth, and apoptosis. In this study, poly(3,4-ethylenedioxythiophene)-poly-(styrenesulfonate) (PEDOT:PSS) conducting polymer (CP)-based bioelectrodes were developed for the simultaneous drug release and real-time biosensing of cancer cell viability. This device can reliably monitor impedance signal changes correlated with cell viability while exhibiting excellent properties for both drug encapsulation and on-demand release.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Kazushige Shiraishi, Parisha P. Shah, Michael P. Morley, Claudia Loebel, Garrett T. Santini, Jeremy Katzen, Maria C. Basil, Susan M. Lin, Joseph D. Planer, Edward Cantu, Dakota L. Jones, Ana N. Nottingham, Shanru Li, Fabian L. Crdenas-Diaz, Su Zhou, Jason A. Burdick, Rajan Jain, Edward E. Morrisey
Summary: Biophysical forces from normal respiratory motion actively maintain alveolar type 1 (AT1) cell identity and restrict their reprogramming into AT2 cells. Cdc42 and Ptk2-mediated actin remodeling and cytoskeletal strain maintain AT1 cell fate, and inactivation of these pathways leads to rapid AT2 cell fate reprogramming. This plasticity induces chromatin reorganization and changes in nuclear lamina-chromatin interactions, which distinguish AT1 and AT2 cell identity. Unloading the biophysical forces of breathing movements results in AT1-AT2 cell reprogramming, highlighting the essential role of normal respiration in maintaining alveolar epithelial cell fate. These findings demonstrate the importance of mechanotransduction in lung cell fate and identify the AT1 cell as a key mechanosensor in the alveolar niche.
Article
Biochemistry & Molecular Biology
Guoxiao Li, Mingji Wei, Jian Sun, Yecheng Zhang, Rongbiao Zhang
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Chemistry, Multidisciplinary
Guoxiao Li, Rongbiao Zhang, Mingji Wei, Changsheng Yin, Jian Sun, Yecheng Zhang
Article
Biotechnology & Applied Microbiology
Mingji Wei, Rongbiao Zhang, Fei Zhang, Yecheng Zhang
Summary: The article proposed a method for evaluating cell viability heterogeneity based on multiphysical property information fusion using polynomial regression. The method extracts data from cell detachment assays and IC50 assays, reducing the mean relative error by 6.04% and 62.79% compared to multiple linear regression and cell counting methods.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Engineering, Electrical & Electronic
Yecheng Zhang, Guohai Liu, Wenxiang Zhao, Huawei Zhou, Qian Chen, Mingji Wei
Summary: The article introduces a new method for diagnosing ISCF in low-speed PMSMs, which improves fault phase identification sensitivity by decomposing zero-sequence voltage vectors and extracting the voltage vector closest to the ISCF phase angle. Additionally, a method for extracting phase voltage from PWM duty-cycle control voltage is proposed to reduce errors caused by measuring zero-sequence voltage with three-phase balanced resistance. Experiments show that the online detection limit of ISCF in low-speed PMSMs is reduced from 5% to 1% (short-circuit ratio) using this method.
IEEE TRANSACTIONS ON TRANSPORTATION ELECTRIFICATION
(2021)
Article
Biochemistry & Molecular Biology
Ming-Ji Wei, Zhen-Nan Wang, Yan Yang, Shu-Jiu Zhang, He Tang, Hui Li, Chong-Liang Bi
Summary: The study found that selenium can significantly reduce the inflammation induced by S. aureus by suppressing the activation of the TLR2 signaling pathway and NLRP3 inflammasome.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Mingji Wei, Fei Zhang, Rongbiao Zhang, Jin-Ming Lin, Ning Yang
Summary: This study introduces a novel method for high-throughput evaluation of cancer cell adhesion strength in long-channel constriction based on friction coefficient. By simplifying the linear elasticity of compressed cells, the influence of cell size heterogeneity is effectively mitigated in determining adhesion strength.
Article
Chemistry, Analytical
Fei Zhang, Rongbiao Zhang, Mingji Wei, Guoxiao Li
Summary: This article proposes a novel approach for evaluating cell migration by distinguishing whether cells have migrated based on the regularity of cell morphology changes. A machine learning based algorithm is used to identify migrated cells using the proposed morphological characteristics. This method greatly improves identification accuracy and could be employed as a potential tool for cell counting and evaluation of cell migration.
Article
Biotechnology & Applied Microbiology
Fei Zhang, Rongbiao Zhang, Mingji Wei, Yecheng Zhang
Summary: A novel cell culture method with automatic feedback based on microfluidic chip was proposed for real-time regulation of cell density. The integrated microfluidic system developed in this study can monitor and control cell density, showing improved consistency and repeatability in repeated assays.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Engineering, Electrical & Electronic
Ning Yang, Qian Shi, Xuejing Zhu, Mingji Wei, Ikram Ullah, Paul Oppong Kwabena, Ekaterina Kulik, Hanping Mao, Rongbiao Zhang
IEEE SENSORS JOURNAL
(2020)