Article
Chemistry, Analytical
Ying Zhang, Bei Gao, Luis Valdiviez, Chao Zhu, Tara Gallagher, Katrine Whiteson, Oliver Fiehn
Summary: Stable isotope tracers are commonly used with gas chromatography coupled to mass spectrometry for enzyme activity and metabolic pathway studies. In this research, three commercially available instruments were used to analyze samples from a stable isotope labeling study on Rothia mucilaginosa metabolism. The study found that all three GC-MS instruments can be used for stable isotope tracing studies, with high-resolution GC-QTOF MS offering additional capabilities in identifying the chemical structures of unknown compounds.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Roque Serrano, Juan Carlos Navarro, Tania Portoles, Carlos Sales, Joaquin Beltran, Oscar Monroig, Felix Hernandez
Summary: The study developed a new methodology for identifying very long-chain polyunsaturated fatty acids (VLC-PUFAs), successfully discovering VLC-PUFAs with chain lengths up to 44 carbons in gilthead sea bream. The research also evaluated the added value of ion mobility-mass spectrometry and the contribution of collisional cross section parameters in characterizing novel VLC-PUFAs.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Applied
Keiko Iwakoshi, Asamoe Ogawa, Katsushi Iwakoshi, Jyunichi Nakajima, Chigusa Kobayashi, Takeo Sasamoto
Summary: A new method for quantifying the brominated vegetable oil content in commercial soft drinks was developed, which demonstrated simple and accurate chromatographic separation techniques and transesterification to improve analytical efficiency and reproducibility. The method also allowed for qualitative confirmation of bromostearic acid methyl esters in the samples via APGC-QTOF, as well as the identification of a novel aqueous ammonium adduct. This method showed high recovery percentages and repeatability values, indicating its effectiveness and reliability.
Article
Biochemical Research Methods
Sonia Abreu, Sylvie Heron, Audrey Solgadi, Bastien Prost, Jessica Dalloux-Chioccioli, Alice Kermarrec, Anne Meynier, Justine Bertrand-Michel, Alain Tchapla, Pierre Chaminade
Summary: This study introduces a new method using atmospheric pressure photoionization and high-resolution mass spectrometry to determine the fatty acyl composition of triacylglycerols and phospholipids in plant oils. The method is highly accurate and reliable, and allows for simultaneous analysis of multiple classes of phospholipids.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Yuka Fujito, Yoshihiro Izumi, Kohta Nakatani, Masatomo Takahashi, Yoshihiro Hayakawa, Mitsuo Takayama, Takeshi Bamba
Summary: Supercritical fluid chromatography (SFC) is often coupled with electrospray ionization mass spectrometry (ESIMS) for analyte detection. The ionization mechanism in SFC/ESI-MS is different from liquid chromatography due to the use of CO2 as a mobile phase. The formation of methoxylcarbonic acid contributes to ion generation and detection in positive-ion mode, but interferes with ionization in negative-ion mode.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Moritz Hitzemann, Christoph Schaefer, Ansgar T. Kirk, Alexander Nitschke, Martin Lippmann, Stefan Zimmermann
Summary: In this paper, a new and easy to assemble dielectric barrier discharge plasma ionization source is presented, which is based on printed circuit boards with two parallel isolated electrodes for generating plasma inside an inert fused silica capillary. The plasma source is coupled to an ion mobility spectrometer for demonstration. Different ionization pathways can be achieved by whether the analytes pass through the plasma or bypass it before entering the reaction region of the ion mobility spectrometer. The plasma source has been designed using electric field simulations and can reach detection limits in the mid pptv range.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Chu -Fan Wang, Liang Li
Summary: Chemical isotope labeling (CIL) LC-MS is a powerful tool for metabolome analysis that offers improved coverage and accuracy. This study compares quadrupole time-of-flight (QTOF) and Orbitrap mass spectrometers for metabolite detection in CIL LC-MS. The results show that instrument type has a significant effect on metabolite detection, and combining data from different instruments can increase the number of detected peak pairs.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Xiaowei Zhang, Xinpeng Diao, Luya Li, Yuqian Zhang, Man Liao, Guohua Zhang, Lantong Zhang
Summary: This study analyzed the metabolic profile of ginkgolide B using ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry, and identified a total of 53 different metabolites. The biotransformation route of ginkgolide B was determined, providing useful information for further research on its pharmacology and mechanism.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Johannes Wellmann, Conny Traenkner, Nicolas Dostert, Esther-Corinna Schwarze, Silke Hillebrand, Jakob P. Ley, Peter Winterhalter
Summary: A workflow for comprehensive semi-polar metabolite profiling was developed and validated using liquid chromatography coupled with electrospray ionization ion mobility quadrupole time-of-flight mass spectrometry. This workflow has the capability to distinguish different cultivars of Hydrangea macrophylla ssp. serrata and obtain a list of metabolites responsible for the differentiation. Suspect screening of phenyldihydroisocoumarins provides valuable information for the biosynthesis of phyllodulcin.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Analytical
Xiao-wei Zhang, Yu-ting Chen, Xue Feng, Lu-ya Li, Ke-wei Song, Yu-peng Sun, Guo-hua Zhang, Lan-tong Zhang
Summary: In this study, the metabolism of celastrol in vivo and in vitro was comprehensively characterized using ultra-high-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry. A total of 43 metabolites were identified and characterized, and it was found that the biotransformation capacity of the intestinal tract exceeded that of the liver. These findings provide a basis for understanding the pharmacological mechanisms of celastrol and have potential implications for clinical applications.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Food Science & Technology
Jian Li, Junmei Ma, Yan Zhang, Lei Zheng
Summary: A rapid method was developed for the determination of 19 polyphenols in tea using ultra-high performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC-Q-TOF MS). The method exhibited simplicity, rapidity, and accuracy, making it suitable for the rapid screening and quantitative analysis of polyphenols in tea.
FOOD SCIENCE AND HUMAN WELLNESS
(2022)
Article
Chemistry, Analytical
Rupa Shakya, Eunsu Cho, Hyun Joo Heo, Dong Won Lee, Jae Seon Kang, Sanggil Choe, Hyung Hoi Kim, Kang Min Kim, Jaesung Pyo
Summary: This study analyzed the metabolites of thioquinapiperifil using LC-Q-TOF-MS and identified multiple metabolites in human and rat samples. The mass error values of all TOF mass results were within 5 ppm, indicating high accuracy. These newly identified metabolites may be useful for evaluating the toxicology of thioquinapiperifil and related forensic cases.
ANALYTICAL LETTERS
(2022)
Article
Food Science & Technology
Shizuka Saito-Shida, Satoru Nemoto, Hiroshi Akiyama
Summary: The study demonstrated the applicability of LC-QTOF-MS method for quantitative and confirmatory analysis of pesticide residues in cereal grains and legumes, with high signal intensities and accuracy, suitable for regulatory routine analysis.
Article
Biochemical Research Methods
Dominik Brecht, Florian Uteschil, Oliver J. Schmitz
Summary: The newly developed argon iLTP ion source for liquid chromatography/tandem mass spectrometry shows promising performance in ionization of small organic molecules, with potential cost savings compared to the traditional helium dielectric barrier discharge (DBD) method. Further investigations are needed to fully understand the mechanism of argon discharge gas.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2021)
Article
Chemistry, Analytical
A. Arrizabalaga-Larranaga, S. Epigmenio-Chamu, F. J. Santos, E. Moyano
Summary: This paper describes the development of a rapid and sensitive method for detecting banned dyes in spice samples using UHPLC-MS/MS technology combined with three atmospheric pressure ionization sources. The proposed method demonstrated good performance in sample analysis, with low detection limits, good precision, and accurate quantitation.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Roque Serrano, Juan Carlos Navarro, Tania Portoles, Carlos Sales, Joaquin Beltran, Oscar Monroig, Felix Hernandez
Summary: The study developed a new methodology for identifying very long-chain polyunsaturated fatty acids (VLC-PUFAs), successfully discovering VLC-PUFAs with chain lengths up to 44 carbons in gilthead sea bream. The research also evaluated the added value of ion mobility-mass spectrometry and the contribution of collisional cross section parameters in characterizing novel VLC-PUFAs.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Physics, Atomic, Molecular & Chemical
W. M. A. Niessen
Summary: The discussion focuses on the fragmentation of protonated and/or deprotonated small-molecule compounds in tandem mass spectrometry after electrospray ionization, with a special emphasis on nitro and halogen-related fragmentation. The research indicates that nitro and halogen moieties may lose a radical, and the chemical surroundings can influence the fragmentation process, further affecting the different molecular fragmentation pathways.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2021)
Article
Chemistry, Analytical
W. M. A. Niessen, M. J. X. Hillebrand, H. Rosing, J. H. Beijnen
Summary: Protein kinases inhibitors are important in treating diseases such as cancer where protein kinase activity plays a role, and bioanalysis using LC-MS-MS method has gained considerable attention. Accurate-m/z analysis is endorsed for structure elucidation of product ions in bioanalytical LC-MS-MS studies.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Environmental Sciences
Diogo Peixoto, Amparo Torreblanca, Susana Pereira, Maria Natividade Vieira, Inmaculada Varo
Summary: Microplastics are found everywhere in the world's oceans and their potential toxic effects on aquatic ecosystems remain unknown. This study evaluated biochemical responses caused by fluorescent red polymer microspheres (FRM) on nauplii and juveniles of Artemia franciscana, revealing that juveniles showed decreased survival after exposure to higher concentrations of FRM, potentially indicating neurotoxic and oxidative damage effects induced by the microplastics. The research highlights the need to understand the mechanisms of microplastics toxicity and their impacts on environmental safety.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Physics, Atomic, Molecular & Chemical
W. M. A. Niessen, H. Rosing, J. H. Beijnen
Summary: This paper discusses the bioanalysis of signal transduction inhibitors (STIs) and their application in liquid chromatography-tandem mass spectrometry, summarizing the investigation and review of the fragmentation of STIs in MS-MS. It provides fragmentation schemes of over 100 STIs, and discusses characteristic fragmentation related to their structural features.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2021)
Article
Chemistry, Analytical
Chiara Maria Vitale, Arjen Lommen, Carolin Huber, Kevin Wagner, Borja Garlito Molina, Rosalie Nijssen, Elliott James Price, Marco Blokland, Frederike van Tricht, Hans G. J. Mol, Martin Krauss, Laurent Debrauwer, Olga Pardo, Nuria Leon, Jana Klanova, Jean-Philippe Antignac
Summary: A set of QA/QC criteria for nontargeted measurement of pesticide exposure markers in human urine has been proposed and applied in the HBM4EU project. The criteria include monitoring signal intensities, mass accuracy, and retention times of selected markers. The results show high repeatability and accuracy of the data. This is the first time that harmonized QA/QC criteria have been used to assess the quality of nontargeted analysis of human samples across multiple laboratories.
ANALYTICAL CHEMISTRY
(2022)
Review
Environmental Sciences
Abel Albiach-Delgado, Francesc A. Esteve-Turrillas, Sandra F. Fernandez, Borja Garlito, Olga Pardo
Summary: Human biomonitoring is a valuable tool for assessing human exposure to acrylamide, with specific and sensitive biomarkers serving as reliable indicators of exposure. This review provides an overview of available knowledge on the HBM of acrylamide, including preferred exposure biomarkers, analytical methods, current data, and tools for interpreting HBM data. Future trends in this field are also discussed.
Article
Chemistry, Analytical
David Izquierdo-Sandoval, David Fabregat-Safont, Leticia Lacalle-Bergeron, Juan V. Sancho, Felix Hernandez, Tania Portoles
Summary: The combination of GC with IMS-HRMS is considered a promising approach for monitoring (semi)volatile compounds in complex matrices. Utilizing soft ionization and ion mobility separation, it enables rapid, sensitive, and efficient wide-scope screening while improving (tentative) identifications. With the assessment of 264 organic pollutants, GC-APCI-IMS-HRMS demonstrated robust identification power in complex-matrix samples.
ANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Carolin Huber, Rosalie Nijssen, Hans Mol, Jean Philippe Antignac, Martin Krauss, Werner Brack, Kevin Wagner, Laurent Debrauwer, Chiara Maria Vitale, Elliott James Price, Jana Klanova, Borja Garlito Molina, Nuria Leon, Olga Pardo, Sandra F. Fernandez, Tamas Szigeti, Szilvia Kozepesy, Libor Sulc, Pavel Cupr, Inese Martinsone, Lasma Akulova, Ilse Ottenbros, Roel Vermeulen, Jelle Vlaanderen, Mirjam Luijten, Arjen Lommen
Summary: Within the HBM4EU initiative, a study was conducted to identify and assess exposure patterns to pesticides using human urine samples. The study applied a harmonized screening approach based on LC-HRMS in five laboratories, resulting in the detection of 14 parent pesticides and 71 metabolites, representing 46 unique pesticides.
ENVIRONMENT INTERNATIONAL
(2022)
Article
Multidisciplinary Sciences
Roque Serrano, Juan C. Navarro, Carlos Sales, Tania Portoles, Oscar Monroig, Joaquin Beltran, Felix Hernandez
Summary: Very long-chain polyunsaturated fatty acids (VLC-PUFA) play an important role in the development of the nervous system, retinal function, and reproductive processes in vertebrates. The effect of high vegetable oil aquafeed formulations on VLC-PUFA levels has not been fully understood.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
D. Aurora Perini, Elisa Parra-Ortiz, Inmaculada Varo, Maria Queralt-Martin, Martin Malmsten, Antonio Alcaraz
Summary: The interaction between nanoplastics and phospholipid membranes can affect membrane integrity and lead to toxic effects.
Article
Biochemistry & Molecular Biology
Jens Boyen, Alberto Ribes-Navarro, Naoki Kabeya, Oscar Monroig, Annelien Rigaux, Patrick Fink, Pascal I. Habluetzel, Juan Carlos Navarro, Marleen De Troch
Summary: Long-chain (>= C-20) polyunsaturated fatty acids (LC-PUFAs) are physiologically important for most animals, including humans. Recent research indicates the ability of certain invertebrates, mainly marine organisms, to synthesize and convert LC-PUFAs. The genetic pathways and mechanisms of LC-PUFA biosynthesis remain unknown in many invertebrates, but existing genomic resources can contribute to our understanding. This study identified key enzymes responsible for LC-PUFA biosynthesis in the copepod Platychelipus littoralis and demonstrated their functionality using heterologous expression in yeast.
Article
Chemistry, Applied
Borja Garlito, Miguel A. Sentandreu, Vicent Yusa, Mamen Olivan, Olga Pardo, Enrique Sentandreu
Summary: Metabolite profiles of normal and defective dry, firm and dark (DFD) meat extracts were analyzed using Orbitrap Tribrid ID-X untargeted analysis coupled to chemometrics. Threonylphenylalanine (overexpressed in normal meats) and tetradecadienoyl- and hydroxydodecanoyl-carnitines (both overexpressed in DFD meats) appropriately grouped meat groups assayed. Results revealed the limitations of pHu as an isolated parameter for accurate meat quality differentiation and suggested interferences induced by pre-slaughter stress (PSS) on lipid catabolism.
Article
Public, Environmental & Occupational Health
Ilse Ottenbros, Erik Lebret, Carolin Huber, Arjen Lommen, Jean-Philippe Antignac, Pavel Cupr, Libor Sulc, Ondrej Mikes, Tamas Szigeti, Szilvia Kozepesy, Inese Martinsone, Zanna Martinsone, Lasma Akulova, Olga Pardo, Sandra F. Fernandez, Clara Coscolla, Susana Pedraza-Diaz, Martin Krauss, Laurent Debrauwer, Kevin Wagner, Rosalie Nijssen, Hans Mol, Chiara Maria Vitale, Jana Klanova, Borja Garlito Molina, Nuria Leon, Roel Vermeulen, Mirjam Luijten, Jelle Vlaanderen
Summary: This study investigated the probability of exposure to pesticide mixtures in five European countries and found that humans are exposed to various pesticides through diet and the environment. Living in an agricultural area, being a child, and the season of urine sampling can affect the detection of pesticides. The study also showed differences in pesticide exposure between adults and children.
INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH
(2023)
Article
Agriculture, Multidisciplinary
Roque Serrano, Eduardo Beltran, Vanesa Piquer, Felix Hernandez, Evaristo Mananos
Summary: Understanding the levels of steroids in fish is crucial for reproductive success in aquaculture. A highly sensitive and reliable analytical methodology has been optimized for the identification and quantification of 13 steroids in different tissues of sole. The developed methodology allows for the identification and quantification of steroids at sub-nanogram per milliner and subnanogram per gram levels in captive fish samples.
ACS AGRICULTURAL SCIENCE & TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)