Article
Microbiology
Mei-nian Xu, Li Li, Wen Pan, Huan-xin Zheng, Meng-lei Wang, Xiao-ming Peng, Si-qi Dai, Ying-mei Tang, Kang Zeng, Xiao-wen Huang
Summary: The study found that low concentrations of ZnO-NPs were non-toxic to Galleria mellonella larvae, and prophylactic injection of ZnO-NPs significantly prolonged the survival of C. albicans-infected larvae while reducing fungal dissemination. The protective effect was attributed more to the activation of host defense mechanisms rather than the direct fungicidal properties of ZnO-NPs.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Thilo Kohler, Alexandre Luscher, Lena Falconnet, Gregory Resch, Robert McBride, Quynh-Anh Mai, Juliette L. Simonin, Marc Chanson, Bohumil Maco, Raphael Galiotto, Arnaud Riat, Shawna McCallin, Benjamin Chan, Christian van Delden
Summary: The study documents the successful treatment of a male patient with life-threatening multi-drug resistant Pseudomonas aeruginosa infection using personalized phage therapy and intravenous antibiotics. This indicates that phage therapy can be an alternative or complementary strategy for multidrug resistant bacterial infections.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Victor Garcia-Bustos, Javier Peman, Alba Ruiz-Gaitan, Marta Dafne Cabanero-Navalon, Ana Cabanilles-Boronat, Maria Fernandez-Calduch, Lucia Marcilla-Barreda, Ignacio A. Sigona-Giangreco, Miguel Salavert, Maria Angeles Tormo-Mas, Amparo Ruiz-Sauri
Summary: This study analyzes the in vivo behavior of Candida auris compared to other Candida species in G. mellonella. It reveals that C. auris exhibits higher immunogenic activity, unique invasion patterns, and inter-phenotypic tissue tropism differences. The study also highlights the importance of phenotypic identification in clinical settings.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Immunology
Aitor Elizalde-Bielsa, Beatriz Aragon-Aranda, Maite Loperena-Barber, Miriam Salvador-Bescos, Ignacio Moriyon, Amaia Zuniga-Ripa, Raquel Conde-Alvarez
Summary: Brucellosis is a zoonotic disease caused by Gram-negative bacteria of the genus Brucella, which can cause long-lasting infections. The use of mice as in vivo models for investigating bacterial virulence is limited due to ethical and practical considerations. Galleria mellonella larvae have been increasingly used as an alternative model, showing lower mortality and delayed death upon Brucella infection compared to mice.
MICROBIAL PATHOGENESIS
(2023)
Article
Zoology
Bartlomiej Iwanski, Mariola Andrejko
Summary: The role of Pseudomonas aeruginosa exotoxin A in modulating humoral immune response parameters in Galleria mellonella larvae was investigated. The results showed that exoA can act as a virulence factor by inhibiting insect immune responses and decreasing the level of specific proteins. This study also provided evidence for the usefulness of using insect models to analyze P. aeruginosa toxins.
JOURNAL OF INVERTEBRATE PATHOLOGY
(2022)
Article
Immunology
Renata Mendonca Moraes, Maira Terra Garcia, Fabio Stossi, Patricia Pimentel de Barros, Juliana Campos Junqueira, Ana Lia Anbinder
Summary: The adrenergic signalling pathway plays an important role in the host immune response and Porphyromonas gingivalis infection. Isoproterenol increases the number of haemocytes in Galleria mellonella and reduces mortality and bacterial recovery after infection, while norepinephrine shows opposite effects.
Article
Environmental Sciences
Benay Tuncsoy, Serkan Sugecti, Ender Buyukguzel, Pinar Ozalp, Kemal Buyukguzel
Summary: The study revealed that dietary CuO nanoparticles negatively impacted metabolic enzyme activity and biochemical parameters in Galleria mellonella larvae, while increasing the total hemocyte counts in their hemolymph. Plasmatocytes and granulocytes were the most numerous types of hemocytes in all treatments.
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
(2021)
Article
Immunology
Masanori Asai, Yanwen Li, John Spiropoulos, William Cooley, David J. Everest, Sharon L. Kendall, Carlos Martin, Brian D. Robertson, Paul R. Langford, Sandra M. Newton
Summary: This study demonstrates the use of Galleria mellonella larvae as a low-cost, rapid, and ethically more acceptable model for tuberculosis research. The Gm-MTB model can mimic key aspects of the disease and can be used for anti-TB drug screening and comparing virulence of different strains.
Article
Biotechnology & Applied Microbiology
Orlando Poma, Betty Ricce, Jeyson Beraun, Jackson Edgardo Perez Carpio, Hugo Fernandez, Juan Soria
Summary: Plastic pollution, resulting from the long decomposition time of most single-use plastics, is a grave environmental problem. This study evaluates the efficacy of G. mellonella in biodegrading LDPE in the district of Pangoa, Junin, Peru. The results suggest that the best treatment is conditioning G. mellonella with beeswax and carrying out the treatment for 36 hours.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Ata Eskin, Zahide Ulya Nurullahoglu
Summary: This study examined the effects of different doses of zinc oxide nanoparticles on the immune response of Greater Wax Moth. The results showed that the nanoparticles caused a decrease in hemocyte count and altered the encapsulation and melanization response of the larvae.
DRUG AND CHEMICAL TOXICOLOGY
(2023)
Article
Infectious Diseases
Enas Mamdouh Hefzy, Mahmoud A. F. Khalil, Amal A. Ibrahim Amin, Hossam M. Ashour, Yasser Fathy Abdelaliem
Summary: The study showed that BLISs from lactobacilli and streptococci have inhibitory effects on Candida species, with good stability against heat, pH, and surfactants but sensitivity to proteases. In vivo testing using Galleria mellonella larvae model demonstrated that BLISs significantly improved survival rates when treated with Candida isolates, suggesting the potential of BLISs as biotherapeutic agents in vulvovaginal candidiasis.
Article
Microbiology
Dan-Tiberiu Furnica, Silke Dittmer, Maike Isabell Sanders, Joerg Steinmann, Peter-Michael Rath, Lisa Kirchhoff
Summary: Aspergillus fumigatus has become a significant threat in clinical settings, with increasing cases of invasive infections caused by azole-resistant A. fumigatus isolates (ARAF). This study investigated the in-vitro and in-vivo activity of the imidazole luliconazole (LLCZ) against clinical ARAF. The results showed that LLCZ has inhibitory effects on the planktonic cells and early biofilms of ARAF.
Article
Immunology
Renee Zakhia, Mike A. Osta
Summary: Clip domain serine proteases and their homologs, cSPHs, play essential roles in the serine protease cascades that drive melanization. This study reveals that CLIPA7, a member of the cSPH family, is crucial for the melanization of Escherichia coli and the clearance of systemic infections with Staphylococcus aureus. The findings provide further insights into the complex functions of cSPHs in mosquito immunity.
JOURNAL OF INNATE IMMUNITY
(2023)
Article
Immunology
Nathalia Ferreira Fregonezi, Lariane Teodoro Oliveira, Junya de Lacorte Singulani, Caroline Maria Marcos, Claudia Tavares dos Santos, Maria Lucia Taylor, Maria Jose Soares Mendes-Giannini, Haroldo Cesar de Oliveira, Ana Marisa Fusco-Almeida
Summary: This study investigated the influence of Hsp60 on biofilm features of H. capsulatum and its effect in the G. mellonella model. The results showed that the Hsp60 antibody (mAb 7B6) was effective in reducing metabolic activity and biomass of the fungus, resulting in thinner biofilms with lower cell and matrix content, as well as increased survival of infected larvae. The findings suggest a different pattern of fungus-host interaction in the invertebrate model compared to a murine model, highlighting the importance of Hsp60 in biofilm establishment and larval infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Laura Moya-Anderico, Joana Admella, Eduard Torrents
Summary: This study presents an optimized and novel protocol for clearing Galleria mellonella larvae, allowing for visualization of internalized fluorescent nanoparticles using confocal microscopy and opening doors to a wide range of applications.
