Article
Engineering, Biomedical
Lais Morandini, Derek Avery, Benjamin Angeles, Paul Winston, Rebecca K. Martin, Henry J. Donahue, Rene Olivares-Navarrete
Summary: Neutrophils play a crucial role in the immune response to biomaterial implantation by releasing cytokines, chemokines, and DNA-based networks called NETs. In this study, we found that inhibiting NET formation suppresses pro-inflammatory macrophage activation and accelerates the inflammatory phase of healing, leading to greater bone formation around the implanted biomaterial. These findings highlight the importance of the neutrophil response and innate immune cell regulation during the integration of biomaterials.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Multidisciplinary
Xuemei Liu, Geng Dou, Zihan Li, Xiangdong Wang, Ronghua Jin, Yao Liu, Huijuan Kuang, Xiaoyao Huang, Xiaoxue Yang, Xiaoshan Yang, Siying Liu, Meiling Wu, Hao Guo, Feng Ding, Haokun Xu, Shiyu Liu, Yan Jin, Kun Xuan
Summary: This study successfully promotes tissue regeneration in critical-sized bone defects and cutaneous wounds by manipulating the inflammatory responses, providing a new strategy for refractory wound therapy.
Article
Biochemistry & Molecular Biology
Joanna M. Poczobutt, Andrew M. Mikosz, Christophe Poirier, Erica L. Beatman, Karina A. Serban, Fabienne Gally, Danting Cao, Alexandra L. McCubbrey, Christina F. Cornell, Kelly S. Schweitzer, Evgeny Berdyshev, Irina A. Bronova, Francois Paris, Irina Petrache
Summary: The deficiency of ASM is associated with a complex inflammatory phenotype in the lungs, characterized by accumulation of specific macrophages and abnormal release of proinflammatory cytokines. This new insight may provide a novel link between ASM and the development of crystal-associated lung inflammation with alterations in macrophage biology.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2021)
Article
Clinical Neurology
Chen Wang, Verena Boerger, Ayan Mohamud Yusuf, Tobias Tertel, Oumaima Stambouli, Florian Murke, Nico Freund, Christoph Kleinschnitz, Josephine Herz, Matthias Gunzer, Aurel Popa-Wagner, Thorsten R. Doeppner, Bernd Giebel, Dirk M. Hermann
Summary: This study demonstrates the neuroprotective and anti-inflammatory effects of MSC-sEVs in aged mice after ischemia, which can reduce neurological deficits, infarct volume, brain edema, neuronal injury, and the infiltration of leukocytes in the brain, as well as decrease the number of monocytes and activated T cells in peripheral blood.
Review
Cell Biology
Jenying Deng, Jason B. Fleming
Summary: Most immunotherapy drugs target T cell surface proteins to enhance their tumor-killing activity, but this approach has limited success in certain solid tumors. Myeloid-related innate immunity plays a role in tumor progression and understanding this field may lead to new therapeutic options. Myeloid cells can differentiate into pro-inflammatory or anti-inflammatory forms depending on the tumor microenvironment. In this review, the relationship between cancer cells and myeloid cells in the tumor immune microenvironment, and the development of immunotherapeutic strategies targeting myeloid-related innate immunity will be discussed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Mani Salarian, Mean Ghim, Jakub Toczek, Jinah Han, Dar Weiss, Bart Spronck, Abhay B. Ramachandra, Jae-Joon Jung, Gunjan Kukreja, Jiasheng Zhang, Deaneira Lakheram, Sung-Kwon Kim, Jay D. Humphrey, Mehran M. Sadeghi
Summary: In this study, the role of matrix metalloproteinase-12 (MMP-12) in abdominal aortic aneurysm was reevaluated. The findings demonstrate that an MMP-12 deficiency leads to adverse vascular remodeling and rupture of abdominal aortic aneurysm through the activation of the complement pathway and neutrophil extracellular traps.
CIRCULATION RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Miguel Camba-Gomez, Oreste Gualillo, Javier Conde-Aranda
Summary: Inflammation is a crucial physiological process for protecting the body from harm, and the resolution process of inflammation is key in the development of chronic inflammatory diseases. This review summarizes recent advances and information related to the resolution process in the context of intestinal inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Endocrinology & Metabolism
Mari van de Vyver
Summary: Inflammation is a vital immune response that defends against harmful bacteria and pathogens, and also plays a crucial role in tissue maintenance and repair. However, under pathological conditions, there can be persistent inflammation without infection, known as sterile metabolic inflammation or metainflammation. This disruption of the immune response is associated with disease progression, immune senescence, and should not be overlooked in clinical management.
JOURNAL OF ENDOCRINOLOGY
(2023)
Article
Engineering, Biomedical
Ysander von Boxberg, Sylvia Soares, Camille Giraudon, Laurent David, Maud Viallon, Alexandra Montembault, Fatiha Nothias
Summary: Direct contact with chitosan-FPHS can modify the polarization of bone marrow-derived macrophages, favoring the generation of anti-inflammatory subtypes. Lower degree of acetylation and higher chitosan concentration promote macrophage polarization towards anti-inflammatory subtypes.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Chemistry, Applied
Lingsam Tea, Frederic Renou, Taco Nicolai
Summary: Commercial chitosan samples with varying molar masses and degrees of acetylation were tested for their ability to stabilize water-in-water emulsions. It was found that higher degrees of acetylation led to faster stabilization, and hydrophobic acetyl units played a crucial role in stability.
CARBOHYDRATE POLYMERS
(2021)
Article
Chemistry, Applied
Chanoong Lim, Dong Soo Hwang, Dong Woog Lee
Summary: The degree of acetylation (DA) of chitosan is crucial for determining its physical, chemical, and biological properties. This study investigated the molecular interactions of chitosan thin films under different molecular weight and DA conditions. Results showed that the interactions of low molecular weight and high molecular weight chitosan were influenced by different factors.
CARBOHYDRATE POLYMERS
(2021)
Article
Immunology
Monica Dominguez, Verena Truemper, Ana Carolina Mota, Bernhard Bruene, Dmitry Namgaladze
Summary: ATP-citrate lyase (ACLY) is a key enzyme involved in metabolic and epigenetic gene regulation. This study investigated the impact of ACLY phosphorylation on its function in human myeloid cells and found that phosphorylation at serine 455 does not affect the enzymatic activity of ACLY. Additionally, loss of ACLY activity may trigger long-term adaptive mechanisms that increase inflammatory responses.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Paco Lopez-Cuevas, Can Xu, Charlotte E. Severn, Tiah C. L. Oates, Stephen J. Cross, Ashley M. Toye, Stephen Mann, Paul Martin
Summary: This study demonstrates that silencing or knocking out a specific immune cell-expressed miRNA, microRNA-223 (miR223), can reduce cancer progression. By using miniature artificial protocells (PCs) to deliver anti-inflammatory miRNAs, the interactions between immune cells and cancer cells are altered, resulting in reduced tumor burden.
Article
Chemistry, Applied
Shubhang Bhardwaj, Nishi Kant Bhardwaj, Yuvraj Singh Negi
Summary: Chitosan, as a renewable biopolymer, can be applied on the surface of writing and printing grade paper to enhance its properties. The degree of acetylation (DA) of chitosan has a significant impact on its performance in various applications, with high DA chitosan outperforming low DA chitosan in some properties. Overall, chitosan with different DA levels has diverse effects on the individual properties of paper.
CARBOHYDRATE POLYMERS
(2021)
Article
Biochemistry & Molecular Biology
Murilo A. Vigilato Rodrigues, Crisiane A. Marangon, Virginia da C. Amaro Martins, Ana Maria de Guzzi Plepis
Summary: The inclusion of jatoba resin and modification of chitosan DA significantly improved the properties of chitosan/gelatin films, such as antimicrobial activity and barrier properties. By adjusting these parameters, the properties of the films can be better controlled.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Biochemistry & Molecular Biology
Francesca Cuomo, Silvio Iacovino, Pasquale Sacco, Antonella De Leonardis, Andrea Ceglie, Francesco Lopez
Summary: This review presents recent advances in the delivery of bioactive phenolic compounds mediated by colloid systems. The focus is on the progress made in this field in recent years. The structural and dynamic aspects of different colloid delivery systems, as well as the interactions with two bioactive constituents, are discussed. Two case studies are presented, one representing hydrophobic phenolic compounds and the other representing hydrophilic compounds.
Article
Chemistry, Multidisciplinary
Pasquale Sacco, Francesco Piazza, Chiara Pizzolitto, Gabriele Baj, Francesco Brun, Eleonora Marsich, Ivan Donati
Summary: This study presents a strategy to control the dissipation rate of viscoplastic substrates while precisely regulating linear elasticity, and reveals the crucial link between this material property and cellular responses in 2D cultures.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Correction
Biochemical Research Methods
Qiqian Liu, Pasquale Sacco, Eleonora Marsich, Franco Furlani, Celia Arib, Nadia Djaker, Marc Lamy de la Chapelle, Ivan Donati, Jolanda Spadavecchia
BIOCONJUGATE CHEMISTRY
(2022)
Article
Chemistry, Applied
Chiara Pizzolitto, Fabiana Esposito, Pasquale Sacco, Eleonora Marsich, Valentina Gargiulo, Emiliano Bedini, Ivan Donati
Summary: In this study, lactose-modified chitosan was sulfated using SO3.py or SO3.DMF as sulfating agents. The extent and selectivity of chemical sulfation were determined using various experimental methods. The sulfated samples were found to induce aggregation of human bone marrow mesenchymal stem cells, resulting in smaller nodules.
CARBOHYDRATE POLYMERS
(2022)
Article
Chemistry, Applied
Giovanni Ferraro, Emiliano Fratini, Pasquale Sacco, Fioretta Asaro, Francesca Cuomo, Ivan Donati, Francesco Lopez
Summary: This study investigated the effect of biomacromolecule concentration and ageing on the structural integrity of chia mucilage-based hydrogel networks. The results showed that ageing destabilizes the hydrogel architecture, leading to a decrease in strength and viscosity, as well as an increase in mesh size.
FOOD HYDROCOLLOIDS
(2022)
Article
Biochemistry & Molecular Biology
Franco Furlani, Elisabetta Campodoni, Nicola Sangiorgi, Monica Montesi, Alessandra Sanson, Monica Sandri, Silvia Panseri
Summary: This study develops electroconductive scaffolds for cardiac tissue regeneration using a biocompatible and conductive polymer (PEDOT:PSS) combined with a biomimetic polymer network of gelatin. The scaffolds, fabricated through dehydrathermal (DHT) treatment, mimic the mechanical properties of native cardiac tissue, have well-interconnected porosity, and exhibit good stability in physiological conditions. The presence of PEDOT:PSS enhances the electroconductivity of the scaffolds and promotes cell adhesion, proliferation, and spreading. These biomimetic and electroconductive scaffolds have potential applications as implantable biomaterials for regenerating electroconductive tissues, especially cardiac tissue, and as 3D tissue models for in vitro biomolecules screening.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Biochemistry & Molecular Biology
Silvina Guidugli, Myriam Villegas, Julio Benegas, Ivan Donati, Sergio Paoletti
Summary: Experimental and theoretical data were used to study the hydration and chain expansion of pectic acid upon charging. The theoretical predictions were confirmed by experimental measurements, showing a high level of accuracy. The relevance of hydration to the bonding mechanism of calcium ions by sodium pectate was discussed. The results also indicated that charged chains are more expanded than neutral chains under typical conditions of ionic strength.
BIOPHYSICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Chiara Pizzolitto, Francesca Scognamiglio, Pasquale Sacco, Sara Lipari, Maurizio Romano, Ivan Donati, Eleonora Marsich
Summary: This study investigated the effect of different viscoplastic ECM mimics on the osteogenic engagement of MSCs. By developing a soft hydrogel, it was found that the presence of temporary cross-links led to early osteogenic commitment of MSCs, while purely elastic hydrogels required a longer time for differentiation to occur.
CARBOHYDRATE POLYMERS
(2023)
Article
Materials Science, Multidisciplinary
Chiara Pizzolitto, Francesca Scognamiglio, Giovanna Baldini, Roberta Bortul, Gianluca Turco, Ivan Donati, Vanessa Nicolin, Eleonora Marsich
Summary: Chondrocyte-based aggregates were formed by culturing cells on a polymeric coating of a lactose-modified chitosan (CTL). The formation of these aggregates was mainly through cell aggregation rather than proliferation, resulting in structures with an irregular morphology up to 1 mm in size. The lactose-modified chitosan (CTL) acted as a temporary matrix for chondrocyte aggregation during the formation of chondro-aggregates, allowing for innovative therapeutic approaches in cartilage regeneration without the need for a scaffold.
ACS APPLIED POLYMER MATERIALS
(2023)
Article
Engineering, Biomedical
Francesco Piazza, Pietro Parisse, Julia Passerino, Eleonora Marsich, Luca Bersanini, Davide Porrelli, Gabriele Baj, Ivan Donati, Pasquale Sacco
Summary: The rate of cooling after heating is crucial for the structure and performance of agarose hydrogel networks. This study presents a material strategy for the fine modulation of quenching during the temperature-curing steps of agarose, revealing insights into the accumulation and recovery of agarose on the surface. The temperature curing of agarose allows for the production of networks with tunable mechanics and is suitable for mechanobiology studies.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Engineering, Multidisciplinary
Francesco Piazza, Matilde Colella, Giuseppe Cinelli, Francesco Lopez, Ivan Donati, Pasquale Sacco
Summary: This study investigates the structural changes of chia seed mucilage when treated with alpha-amylase, the most abundant enzyme in human saliva. The results reveal that the material thickens and the elastic modulus increases after enzyme treatment, indicating a restructuring of the chia mucilage network mediated by alpha-amylase. This finding has implications for the commercial processing of chia seed mucilage.
Correction
Chemistry, Multidisciplinary
Qiqian Liu, Hui Liu, Pasquale Sacco, Nadia Djaker, Marc Lamy de la Chapelle, Eleonora Marsich, Xiaowu Li, Jolanda Spadavecchia
NANOSCALE ADVANCES
(2022)
Meeting Abstract
Cell & Tissue Engineering
Pasquale Sacco, Chiara Pizzolitto
TISSUE ENGINEERING PART A
(2022)
Article
Polymer Science
Lorenzo Mio, Pasquale Sacco, Ivan Donati
Summary: This study examines the effect of temperature and polymer concentration on the strain hardening of chitosan hydrogels. The results show that the extent of hardening can be modulated by varying the gel temperature, but is independent of other experimental conditions. The study suggests that this effect is due to a delicate balance between physical entanglements and covalent crosslinks in highly acetylated chitosan hydrogels under large deformations.
Article
Materials Science, Biomaterials
Franco Furlani, Margherita Montanari, Nicola Sangiorgi, Emanuela Saracino, Elisabetta Campodoni, Alessandra Sanson, Valentina Benfenati, Anna Tampieri, Silvia Panseri, Monica Sandri
Summary: This work presents the development of electroconductive hydrogels as injectable matrices for neural tissue regeneration. The physical-chemical properties of the hydrogels can be finely tuned by the amount of conductive polymer and natural cross-linking agent used in the formulation. The resulting hydrogels exhibit enhanced adhesion and growth of primary cortical astrocytes, indicating potential applications in neurological therapies and brain tissue repair.
BIOMATERIALS SCIENCE
(2022)