Sulfated lactose-modified chitosan. A novel synthetic glycosaminoglycan-like polysaccharide inducing chondrocyte aggregation

Lactose-modified chitosan (CTL) is sulfated using SO3.py or SO3.DMF as sulfating agents. The two products are characterized by elemental analysis, FT-IR, H-1,C-13-DEPT-HSQC and H-1,C-13-HSQC-TOCSY experiments which allow the extent and selectivity of chemical sulfation to be determined. Dynamic Light Scattering shows a pH-dependent association of the sulfated polysaccharides which are described as flexible by the Smidsrod's B parameter and the intrinsic viscosity at infinite ionic strength. Shear viscosity and intrinsic viscosity show that sulfation protocols lead to chain scission which is more pronounced when SO3.DMF is used. The sulfated samples are able to induce aggregation of human bone marrow mesenchymal stem cells, resulting in the formation of smaller nodules compared to the unmodified CTL sample. Over time, the sample with the higher degree of sulfation allows further aggregation between cell clusters while the sample with the lower degree of sulfation shows dissolution of the aggregates.

Automated summary

In this study, lactose-modified chitosan was sulfated using SO3.py or SO3.DMF as sulfating agents. The extent and selectivity of chemical sulfation were determined using various experimental methods. The sulfated samples were found to induce aggregation of human bone marrow mesenchymal stem cells, resulting in smaller nodules.