Article
Plant Sciences
Yanwei Li, Ying Wang, Xinyi Wu, Jian Wang, Xiaohua Wu, Baogen Wang, Zhongfu Lu, Guojing Li
Summary: Fusarium wilt is a soil-borne disease that affects bottle gourd yield and fruit quality. Through GWAS analysis, potential candidate genes for FW resistance were identified, providing scientific evidence for breeding programs.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Microbiology
Xiao-Min Dong, Qing-Gui Lian, Jing Chen, Rui-Min Jia, Zhao-Feng Zong, Qing Ma, Yang Wang
Summary: This study found that the improved biocontrol agent, F1-35, has a high control efficiency of 61.7% against watermelon Fusarium wilt. F1-35 improves the resistance of watermelons to the pathogen by triggering the jasmonic acid and ethylene pathways.
Article
Multidisciplinary Sciences
Linxuan Li, Tingting Zhu, Yun Song, Li Feng, Philip James Kear, Rooallah Saberi Riseh, Mahmoud Sitohy, Raju Datla, Maozhi Ren
Summary: This study explored the potential mechanisms of salicylic acid (SA) as a biofungicide by using the highly pathogenic Fusarium oxysporum as a model system. The results showed that SA could inhibit key targets of the pathogen, modify the metabolic activity within pathogen cells, and induce pathogen cell death. This research provides insights into the application of SA in controlling various fungal diseases by targeting the SNF1-TORC1 pathway of pathogens.
JOURNAL OF ADVANCED RESEARCH
(2022)
Article
Genetics & Heredity
Shuhan Yang, Qun Ge, Sumei Wan, Zhihao Sun, Yu Chen, Yanfang Li, Qiankun Liu, Juwu Gong, Xianghui Xiao, Quanwei Lu, Yuzhen Shi, Renhai Peng, Haihong Shang, Guodong Chen, Pengtao Li
Summary: This study focused on the identification and characterization of polyphenol oxidase (PPO) genes in cotton. The researchers identified 7, 8, 14, and 16 PPO genes from different cotton species and found that they were mainly located on chromosome 6. Analysis of conserved motifs and nucleotide sequences showed high similarity in gene structure and domains among cotton PPO genes. Quantitative real-time PCR experiments confirmed a strong correlation between PPO activity and resistance to Verticillium wilt (VW) in cotton. The comprehensive analysis of cotton PPO genes contributes to the screening of candidate genes for further biological function studies and enhances our understanding of the molecular genetic basis of cotton resistance to VW.
Article
Materials Science, Textiles
Ying Yu, Hongyu Chen, Yiying Yang, Didong Lou, Chunbo Liang, Hongmei Yuan, Guangwen Wu, Changjun Xu
Summary: This study revealed significant differences in miRNA expression levels during flax stem fiber development, with certain miRNAs potentially playing key roles in the plant hormone signal transduction pathway. The accuracy and reliability of these results were confirmed through qRT-PCR analysis, providing a foundation for future studies on the regulatory mechanisms of miRNAs in flax stem development.
JOURNAL OF NATURAL FIBERS
(2022)
Article
Biochemistry & Molecular Biology
Raja Rajakani, Pravin Prakash, Dolly Ghosliya, Ranjana Soni, Arpita Singh, Vikrant Gupta
Summary: This study reports the extensive identification of miRNAs and their possible targets in Azadirachta indica for the first time, indicating the therapeutic potential of this medicinal tree. The miRNAs identified in neem range in size between 19 and 23 nt, with a potential negative correlation between miRNA expression and their targets.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Sebastian Gnat, Dominik Lagowski, Aneta Nowakiewicz, Aleksandra Troscianczyk, Mariusz Dylag
Summary: This study examined the suitability of six candidate reference genes for gene expression analysis in the dermatophyte Trichophyton verrucosum cultured in different mycological media. SDHA was identified as the most stable housekeeping gene for future qRT-PCR studies on T. verrucosum strains, suggesting it as an additional good candidate for identification targets in the qRT-PCR technique.
Article
Microbiology
Zhen Huang, Huixue Li, Yuming Zhou, Yixue Bao, Zhenzhen Duan, Caixia Wang, Charles A. Powell, Baoshan Chen, Muqing Zhang, Wei Yao
Summary: In this study, 316 candidate secreted effector proteins (CSEPs) in the fungus F. sacchari were identified using bioinformatic prediction approaches. The functions of 163 CSEPs were investigated using a heterogeneous expression system in N. benthamiana. We found that some CSEPs suppressed cell death while others caused cell death. Expression analysis of these CSEPs during F. sacchari infection suggested their involvement in sugarcane-F. sacchari interactions.
Article
Plant Sciences
Hee Won Jeon, Ae Ran Park, Minjeong Sung, Namgyu Kim, Mohamed Mannaa, Gil Han, Junheon Kim, Yeonjong Koo, Young-Su Seo, Jin-Cheol Kim
Summary: Methyl salicylate (MeSA) can induce resistance against pinewood nematode in Pinus densiflora seedlings, thereby reducing the incidence of pine wilt disease.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Han Wang, Zong-Huan Ma, Juan Mao, Bai-Hong Chen
Summary: The study focused on the EXO70 gene family in grape, analyzing their characteristics and expression differences under stress conditions using bioinformatics. The results provided a foundation for further exploration of the resistance mechanisms and potential functions of these genes in grape breeding.
Article
Food Science & Technology
Urszula Wachowska, Wioletta Pluskota, Katarzyna G. Lowacka, Kinga Szablewska-Stuper, Margaret Balcerzak
Summary: The aim of this study was to analyze the concentrations of trichothecenes in durum wheat grain and changes in the F. graminearum transcriptome under the influence of the Debaryomyces hansenii antagonistic yeast strain. The results showed that Debaryomyces hansenii suppressed the spread of F. graminearum and decreased the content of trichothecenes. However, Debaryomyces hansenii had no effect on the biosynthesis pathway of trichothecenes.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Agronomy
Chengjie Tu, Pei Xu, Runhua Han, Jing Luo, Letian Xu
Summary: This study assessed the stability of seven commonly used reference genes in Plagiodera versicolora and analyzed their expression under different biotic and abiotic conditions. The results showed that RPS18 and EF1A were the most reliable reference genes in different developmental stages and treatments.
Article
Multidisciplinary Sciences
Zongzhuan Shen, Linda S. Thomashow, Yannan Ou, Chengyuan Tao, Jiabao Wang, Wu Xiong, Hongjun Liu, Rong Li, Qirong Shen, George A. Kowalchuk
Summary: By analyzing the composition and functional potentials of soil microbiomes, this study identified specific microbial communities and genes associated with the suppression of banana Panama disease caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc4). Pseudomonas was identified as a potential key taxon linked to pathogen suppression, and the microbiomes in suppressive soils displayed a greater incidence of genes related to quorum sensing, biofilm formation, and synthesis of antimicrobial compounds. These findings suggest the importance of specific microbial communities and functional capacities in disease suppression.
Article
Biochemistry & Molecular Biology
Kathannan Sankar, Hyung Joo Yoon, Young Bo Lee, Kyeong Yong Lee
Summary: Bumble bees are important alternative pollinators and model insects. This study identified stable reference genes for the bumble bee species and their different lines and tissues using RT-qPCR procedures and four analysis programs. The results emphasized the need to evaluate the stability of candidate reference genes for any differently designed lines and tissue conditions in bumble bee species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Min-dong Chen, Bin Wang, Yong-ping Li, Mei-juan Zeng, Jian-ting Liu, Xin-ru Ye, Hai-sheng Zhu, Qing-fang Wen
Summary: This study investigated the selection of reference genes in luffa under various abiotic stresses using qRT-PCR analysis, and validated their stability. EF-1 alpha was identified as the most stable overall reference gene, while UBQ, and TUB were suitable for specific stress treatments. The reliability of the selected reference genes was confirmed by analyzing the expression of a target gene in luffa.
SCIENTIFIC REPORTS
(2021)
Article
Agronomy
Shun Song, Funing Ma, Dongmei Huang, Bin Wu, Chaozhi Ma, Jingyang Li, Yujia Li, Qing Wei, Qiurong Sun, Wenquan Wang, Yi Xu
Summary: The study showed that MaPIP1;1 promoter enhances plant salt resistance, with the M-P2 fragment showing the most significant response to high salt stress among all tested constructs. This promoter fragment may be an ideal choice for enhancing plant resistance to salt stress through gene overexpression.
Article
Biochemistry & Molecular Biology
Jingyang Li, Fei Liu, Hua Yu, Yuqi Li, Shiguang Zhou, Yuanhang Ai, Xinyu Zhou, Youmei Wang, Lingqiang Wang, Liangcai Peng, Yanting Wang
Summary: This study identified a desirable banana crop containing large amounts of edible carbohydrates and digestible lignocellulose, leading to high bioethanol yields. Chemical analysis indicated that the cellulose CrI and lignin G-monomer are major recalcitrant factors affecting biomass enzymatic saccharification in banana pseudostems and rachis.
Article
Plant Sciences
Xiaoyi Wang, Anbang Wang, Yujia Li, Yi Xu, Qing Wei, Jiashui Wang, Fei Lin, Deyong Gong, Fei Liu, Yanting Wang, Liangcai Peng, Jingyang Li
Summary: This study identified a stably inherited mutant RF1 with a semi-dwarfing phenotype through EMS mutagenesis in combination with the FJ cultivar, demonstrating the power of genetic strategy in fruit crop breeding and providing insight into improving agronomic traits in bananas and beyond.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Leiming Wu, Mingliang Zhang, Ran Zhang, Haizhong Yu, Hailang Wang, Jingyang Li, Youmei Wang, Zhen Hu, Yanting Wang, Zi Luo, Lin Li, Lingqiang Wang, Liangcai Peng, Tao Xia
Summary: The study identified a novel rice mutant encoding a R2R3-MYB transcription factor and generated RNAi-silenced transgenic lines for OsMYB103L. It found significantly reduced cellulose levels and altered cellulose nanofibers assembly in the mutant and RNAi lines, leading to enhanced biomass enzymatic saccharification. Additionally, RNA sequencing and DAP-seq analyses indicated that OsMYB103L may specifically mediate cellulose biosynthesis and microfibril assembly.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Multidisciplinary Sciences
Muhammad Aleem Ashraf, Xiaoyan Feng, Xiaowen Hu, Fakiha Ashraf, Linbo Shen, Muhammad Shahzad Iqbal, Shuzhen Zhang
Summary: In this study, a comprehensive evaluation of sugarcane miRNAs for silencing the SCBV genome was conducted. Fourteen potential candidate miRNAs were screened out and the hybridization site of sof-miR159e was predicted at common locus 5534. The predicted data provide useful information for the development of SCBV-resistant sugarcane plants.
Article
Chemistry, Multidisciplinary
Anbang Wang, Jingyang Li, Arwa Abdulkreem AL-Huqail, Mohammad S. AL-Harbi, Esmat F. Ali, Jiashui Wang, Zheli Ding, Saudi A. Rekaby, Adel M. Ghoneim, Mamdouh A. Eissa
Summary: The study found that foliar application of CH-NPs significantly improved the growth and cold stress resistance of banana plants, increasing their weight and showing a positive correlation with photosynthesis pigments and antioxidant enzyme activities. CH-NPs reduced levels of malondialdehyde and reactive oxygen species in the plants, indicating a decrease in oxidative stress.
Article
Biochemistry & Molecular Biology
Shun Song, Dahui Zhang, Funing Ma, Wenting Xing, Dongmei Huang, Bin Wu, Jian Chen, Di Chen, Binqiang Xu, Yi Xu
Summary: This study identified and classified 27 AQP genes from the passion fruit genome. It analyzed the transcriptome expression patterns of these genes under different abiotic stresses and found one AQP gene that improved drought resistance in transgenic Arabidopsis plants. The study provides a foundation for further research on the functions of AQPs in passion fruit.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Dongmei Huang, Funing Ma, Bin Wu, Wenhui Lv, Yi Xu, Wenting Xing, Di Chen, Bingqiang Xu, Shun Song
Summary: In this study, the LOX gene family in passion fruit was analyzed and a candidate gene, PeLOX4, potentially involved in fruit ripeness and the formation of volatile aroma compounds was identified. The expression level of PeLOX4 and LOX enzyme activity increased with fruit ripening, promoting the synthesis of volatile esters in the fruit pulp.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Yi Xu, Wei Hu, Shun Song, Xiaoxue Ye, Zehong Ding, Juhua Liu, Zhuo Wang, Jingyang Li, Xiaowan Hou, Biyu Xu, Zhiqiang Jin
Summary: Adverse environmental factors have a significant impact on crop productivity, so improving crop resistance to multiple stressors is crucial. This study focuses on MaDREB1F in bananas and reveals its common mechanism in conferring resistance to cold and drought stress. MaDREB1F enhances resistance by modulating metabolite levels, activating antioxidant systems, and promoting hormone synthesis, providing important insights for engineering cold- and drought-tolerant crops.
HORTICULTURE RESEARCH
(2023)
Article
Agriculture, Multidisciplinary
Yi Xu, Dong-mei Huang, Fu-ning Ma, Liu Yang, Bin Wu, Wen-ting Xing, Pei-guang Sun, Di Chen, Bing-qiang Xu, Shun Song
Summary: Passion fruit extract contains flavonoids and terpenoids, which have good anti-anxiety and anti-inflammatory effects in humans. The RNA-Seq analysis of the 'Zixiang' cultivar identified potential candidate genes involved in the synthesis pathways of flavonoids and terpenoids in passion fruit. Further functional analysis will provide more insight into the biosynthesis of these compounds.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Plant Sciences
Yan-shu Zhang, Yi Xu, Wen-ting Xing, Bin Wu, Dong-mei Huang, Fu-ning Ma, Ru-lin Zhan, Pei-guang Sun, Yong-yan Xu, Shun Song
Summary: The study focuses on the resistance mechanisms of the MYB gene in passion fruit under different environmental stresses. Through the analysis of MYB gene expression characteristics, two passion fruit varieties with different resistance levels were selected. This research provides insights into the adaptation and resistance of plants to environmental stresses.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Yi Xu, Pengfei Li, Funing Ma, Dongmei Huang, Wenting Xing, Bin Wu, Peiguang Sun, Binqiang Xu, Shun Song
Summary: In this study, 25 members of the PeNAC gene family were identified from the passion fruit genome, and their functions under abiotic stress and different fruit-ripening stages were analyzed. The results showed that PeNAC-19 was significantly induced by abiotic stress and could improve low temperature tolerance. This study not only improved the understanding of the characteristics and evolution of the PeNAC gene family, but also provided new insights into the regulation of the PeNAC gene at different stages of fruit maturation and abiotic stresses.
Article
Multidisciplinary Sciences
Funing Ma, Hongwu Zhou, Yi Xu, Dongmei Huang, Bin Wu, Wenting Xing, Di Chen, Bingqiang Xu, Shun Song
Summary: In this study, 56 putative bZIP members were identified in passion fruit and analyzed using bioinformatics. The transcriptome analysis revealed their response to various stresses, and the expression of four representative members was validated in different tissues and cultivars. The association analysis of transcriptome and metabolome further identified the consistent expression trends and differences in metabolites between PeZIP20 and PeZIP21. This research provides important insights into the functions and resistance properties of bZIPs, as well as the development of novel germplasm.
Article
Horticulture
Yi Xu, Weidong Zhou, Funing Ma, Dongmei Huang, Wenting Xing, Bin Wu, Peiguang Sun, Di Chen, Binqiang Xu, Shun Song
Summary: In this study, a systemic analysis of the passion fruit bHLH (PebHLH) gene family was conducted, revealing 117 PebHLH genes related to plant stress resistance and development. The expression levels of these genes vary under different abiotic stresses and ripening stages. Specifically, PebHLH56 gene was induced under cold stress. This research provides new insights into the regulatory functions of PebHLH genes during fruit maturity stages and abiotic stress.
Article
Chemistry, Physical
Fei Liu, Jingyang Li, Hua Yu, Yuqi Li, Yanting Wang, Hairong Gao, Hao Peng, Zhen Hu, Hailang Wang, Guifen Zhang, Yuanyuan Tu, Liangcai Peng
Summary: This study demonstrates that optimized green liquor and liquid hot water pretreatments can lead to high bioethanol yields from banana pseudostem residues. The research also proposes a mechanism model to explain why banana lignocellulose underwent complete enzymatic saccharification under mild green-like pretreatments.
SUSTAINABLE ENERGY & FUELS
(2021)