Article
Engineering, Biomedical
Gyunggyu Lee, Hyemin Kim, Ji Young Park, Gyeongmin Kim, Jiyou Han, Seok Chung, Ji Hun Yang, Jang Su Jeon, Dong-Hun Woo, Choongseong Han, Sang Kyum Kim, Han-Jin Park, Jong-Hoon Kim
Summary: Advances in pluripotent stem cell technology have led to the efficient and reproducible generation of hepatocyte-like cell spheroids in various sizes using microwell culture platforms. These spheroids show high expressions of hepatic marker genes, enhanced drug-metabolizing activity, and increased sensitivity to hepatotoxicants compared to traditional hepatocytes. This imaging-based toxicity screening system holds promise for predicting drug-induced hepatotoxicity in the early stages of drug discovery.
Review
Pharmacology & Pharmacy
Gurleen Kour, Syed Assim Haq, Bijender Kumar Bajaj, Prem N. Gupta, Zabeer Ahmed
Summary: Phytochemicals have long been known for their therapeutic potential in autoimmune disorders such as rheumatoid arthritis, but their combination with DMARDs can increase the efficacy of treatment while reducing toxic side effects. Studies have shown promising results in experimental and clinical settings when combining phytochemicals with DMARDs, highlighting the importance of this approach in future RA therapy.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Rheumatology
Delila Singh, Nina Hesse, Alla Skapenko, Hendrik Schulze-Koops
Summary: Methotrexate is associated with rare bone lesions that are often misdiagnosed as osteoporotic fractures. Early diagnosis is crucial for treatment and prevention of further osteopathology. This case highlights the importance of raising awareness and taking appropriate therapeutic measures, including discontinuing methotrexate.
Article
Dermatology
Joel M. Gelfand, Joy Wan, He Zhang, Daniel B. Shin, Alexis Ogdie, Maha N. Syed, Alexander Egeberg
Summary: Patients with psoriatic disease, especially psoriasis, are at a higher risk of developing liver disease outcomes compared to those with rheumatoid arthritis when receiving methotrexate. Meanwhile, patients with psoriatic arthritis also have an increased likelihood of developing mild liver disease and cirrhosis.
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY
(2021)
Article
Rheumatology
Paul Studenic, Daniel Aletaha, Maarten de Wit, Tanja A. Stamm, Farideh Alasti, Diane Lacaille, Josef S. Smolen, David T. Felson
Summary: This study aimed to externally validate a revised Boolean remission criteria using a higher patient global assessment (PtGA) threshold and validate the provisionally endorsed index-based criteria. The results showed that using the higher PtGA threshold increased the proportion of patients classified as achieving remission under the "Boolean2.0" criteria, improved agreement with index-based criteria, and did not compromise predictive value for radiographic or functional outcomes. The study findings were endorsed by ACR and EULAR.
ANNALS OF THE RHEUMATIC DISEASES
(2023)
Review
Pharmacology & Pharmacy
Sushmita Negi, Nikunj Tandel, Prakriti Sharma, Rajinder Kumar, Rajeev K. Tyagi
Summary: This manuscript reviews the importance of the combination drug regimen of methotrexate (MTX) and aceclofenac (ACL) in treating and managing rheumatoid arthritis (RA). The combination treatment can regulate immunological pathways and decrease inflammation associated with RA. It has been shown to regulate the signaling pathway controlled by NF-KB and FOXO1.
DRUG DISCOVERY TODAY
(2023)
Article
Medicine, Research & Experimental
Jinzhang Gao, Ning Xiao, Qianlei Wang, Zhengkun Xu, Feng Xiao, Zhaoyi Yang, Wei Wei, Chun Wang
Summary: The study found that OAT3 plays a crucial role in the development of MTX resistance in the treatment of rheumatoid arthritis, serving as a potential target to improve the resistance.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Estela Villanueva-Badenas, M. Teresa Donato, Laia Tolosa
Summary: Hepatotoxicity or drug-induced liver injury (DILI) is a significant problem in drug development, leading to drug failure and regulatory measures. The development of a co-culture system involving hepatocytes and other cell types has been shown to enhance the understanding and prediction of iDILI. In this study, a co-culture system using HepG2 cells and THP-1-derived macrophages was established and used to evaluate the effects of model iDILI compounds on viability and oxidative stress induction.
Article
Biochemistry & Molecular Biology
Eleanor Luce, Clara Steichen, Soumeya Abed, Anne Weber, Philippe Leboulch, Leila Maouche-Chretien, Anne Dubart-Kupperschmitt
Summary: The study demonstrates that siPSCs can differentiate into liver cells and self-organize into 3D structures, facilitating research on liver development and the development of co-culture systems.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Jianyu He, Jinglin Wang, Yuan Pang, Hang Yu, Xueqian Qin, Ke Su, Tao Xu, Haozhen Ren
Summary: The 3D bioprinted hepatic tissue model demonstrated excellent biofunctional and drug-responsive performance, which could potentially be applied in in vitro toxicological studies.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2022)
Article
Rheumatology
Renske C. F. Hebing, Marry Lin, Maja Bulatovic Calasan, Ittai B. Muller, Sohaila Mahmoud, Sandra Heil, Eduard A. Struys, Bart J. F. van den Bemt, Jos W. R. Twisk, Willem Lems, Michael T. Nurmohamed, Gerrit Jansen, Robert de Jonge
Summary: This study investigates the pharmacokinetics of methotrexate polyglutamate (MTX-PG) accumulation in red blood cells (RBCs) and peripheral blood mononuclear cells (PBMCs) in patients with early rheumatoid arthritis (RA) after oral and subcutaneous MTX treatment. The results show that there are disparate pharmacokinetic profiles between PBMCs and RBCs, with higher accumulation of MTX-PG in PBMCs. The distribution profile of MTX-PG in PBMCs remains constant over 6 months, while in RBCs, the main PG-moiety changes from MTX-PG(1) to MTX-PG(3).
ANNALS OF THE RHEUMATIC DISEASES
(2023)
Article
Chemistry, Multidisciplinary
Moonkyoung Jeong, Yuna Jung, Junyong Yoon, Jinyoung Kang, Seo Hyeon Lee, Woojin Back, Hyoyeon Kim, Michael J. Sailor, Dokyoung Kim, Ji-Ho Park
Summary: A porous silicon-based nanomedicine is developed to deliver an antirheumatic drug to inflamed synovium and degrade into bone-remodeling products. The nanomedicine shows immunomo-dulatory and antiresorptive effects, and effectively ameliorates the progression of rheumatoid arthritis in a mouse model.
Article
Medicine, General & Internal
Borja Hernandez-Breijo, Claudia M. Brenis, Chamaida Plasencia-Rodriguez, Ana Martinez-Feito, Marta Novella-Navarro, Dora Pascual-Salcedo, Alejandro Balsa
Summary: The study found that TNFi survival did not depend on seropositivity status, but seropositive patients who received concomitant MTX had significantly longer median survival time. Additionally, the co-administration of prednisone in seropositive patients receiving TNFi was highly associated with TNFi discontinuation.
FRONTIERS IN MEDICINE
(2021)
Article
Chemistry, Analytical
Junqing He, Junyan Wang, Min Zhang, Guoyue Shi
Summary: As a folate antagonist, methotrexate (MTX) has been widely used in clinics for treating various tumors and inflammatory diseases. However, the dosage and clearance of MTX vary between individuals, and it may cause life-threatening side effects at high doses. Therefore, a convenient and simple method for MTX sensing is highly demanded.
Article
Immunology
Liyan Mei, Zhihua Yang, Xiaolin Zhang, Zehao Liu, Maojie Wang, Xiaodong Wu, Xiumin Chen, Qingchun Huang, Runyue Huang
Summary: This study investigated the changes in gut microbiome of RA patients treated with HQT or LEF, revealing significant differences in gut microbiota between RA patients and healthy controls. The clinical efficacy of the two treatments was similar, but the response trends of RA-related clinical indices differed between the two treatment groups.
FRONTIERS IN IMMUNOLOGY
(2021)
