Article
Immunology
Jiawei Zeng, Yao Li, Linrui Jiang, Ling Luo, Yue Wang, Hao Wang, Xiaonan Han, Jian Zhao, Guanglei Gu, Min Fang, Qingrui Huang, Jinghua Yan
Summary: Current mpox outbreaks in non-endemic regions are a global public health concern. Two multi-antigen mRNA vaccine candidates, Rmix4 and Rmix6, were developed using a simplified manufacturing strategy and showed promising results in inducing potent immune responses against the virus. Both candidates protected mice from lethal virus challenge and revealed vulnerability to viral immune evasion.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Virology
Hyun-Seok Oh, Jong Won Yoon, Sanghyun Lee, Soo-Ok Kim, Sun Pyo Hong
Summary: A new Vero cell-adapted Zika virus strain was established and a purified inactivated virus vaccine was developed, showing good vaccine efficacy with a strong immune response.
Article
Veterinary Sciences
Chen Chang, Haiyan Wang, Tao Hua, Daohua Zhang, Weibin Hong, Bihua Deng, Bo Tang
Summary: Adding Astragalus saponins as an immunopotentiator improves the immune efficacy of the PRV inactivated vaccine and reduces the number of immunizations required.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Immunology
Yonghong Liu, Shirin Strohmeier, Irene Gonzalez-Dominguez, Jessica Tan, Viviana Simon, Florian Krammer, Adolfo Garcia-Sastre, Peter Palese, Weina Sun
Summary: By generating and sequentially vaccinating mice with inactivated influenza B viruses displaying mosaic HAs, researchers have successfully induced long-lasting and cross-protective antibody responses. These findings represent a significant step towards the development of a universal influenza B virus vaccine.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Weiwei Zeng, Yingying Wang, Huzi Hu, Qing Wang, Sven M. Bergmann, Yahui Wang, Bo Li, Yuefeng Lv, Hua Li, Jiyuan Yin, Yingying Li
Summary: The study evaluated the immunological effects and protective efficacy of formaldehyde- and beta-propiolactone-inactivated vaccines against TiLV in tilapia, with and without the adjuvant Montanide IMS 1312 VG. Beta-propiolactone-inactivated vaccine showed higher protection efficacy against virus challenge compared to formaldehyde, generating specific IgM and neutralizing antibodies against TiLV. The vaccine also significantly lowered viral loads, increased survival rates, and stimulated a protective immune response, indicating potential for inhibiting viral proliferation.
Article
Fisheries
Cuneyt Tamer, Abdullah Cavunt, Yuksel Durmaz, Emre Ozan, Hamza Kadi, Gulnur Kalayci, Buket Ozkan, Hakan Isidan, Harun Albayrak
Summary: Infectious pancreatic necrosis (IPN) is a highly contagious disease causing high mortality in juvenile trouts, and early diagnosis and prevention are crucial. In Turkey, where there are no commercial licensed vaccines against IPNV, attempts have been made to produce recombinant VP2 subunit vaccine (IPNV-VP2) and inactivated whole particle virus vaccine (IPNV-WPV) from local isolates. The results showed that IPNV-WPV vaccine stimulates immune response faster and has a higher relative percent of Survive (RPS) compared to IPNV-VP2 vaccine in rainbow trout.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Immunology
Jessamine E. Hazlewood, Bing Tang, Kexin Yan, Daniel J. Rawle, Jessica J. Harrison, Roy A. Hall, Jody Hobson-Peters, Andreas Suhrbier
Summary: A chimeric flavivirus vaccine based on Binjari virus was developed and utilized to generate a chimeric ZIKV vaccine. Vaccination with the unadjuvanted chimeric vaccine induced long-lasting neutralizing antibody responses and provided full protection against ZIKV infection in mice.
Article
Virology
Daria Mezhenskaya, Irina Isakova-Sivak, Victoria Matyushenko, Svetlana Donina, Andrey Rekstin, Konstantin Sivak, Kirill Yakovlev, Anastasia Katelnikova, Kirill Kryshen, Valery Makarov, Larisa Rudenko
Summary: Experimental studies in a preclinical ferret model showed that the LAIV/HA+4M2e universal influenza vaccine candidate had higher immunogenicity and cross-protective potential, mainly mediated by M2e-targeted antibodies. Therefore, the LAIV/HA+4M2e vaccine is promising for further evaluation in future clinical trials.
Article
Microbiology
Meng Hao, Ting Bian, Guangcheng Fu, Yi Chen, Ting Fang, Chuanyi Zhao, Shuling Liu, Changming Yu, Jianmin Li, Wei Chen
Summary: This study focuses on Rift valley fever virus (RVFV), a bunyavirus transmitted by mosquitoes that causes severe diseases in animals and humans. The researchers developed a potential vaccine and tested its immunogenicity and protective efficacy in mice, showing promising results. However, further research is needed to confirm its efficacy in a natural animal host and to potentially progress as a vaccine candidate for humans.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Chengjin Ye, Jun-Gyu Park, Kevin Chiem, Piyush Dravid, Anna Allue-Guardia, Andreu Garcia-Vilanova, Paula Pino Tamayo, Vinay Shivanna, Amit Kapoor, Mark R. Walter, James J. Kobie, Richard K. Plemper, Jordi B. Torrelles, Luis Martinez-Sobrido
Summary: This study confirms the feasibility of attenuating SARS-CoV-2 through the generation of recombinant viruses lacking two accessory ORF proteins. These double ORF-deficient viruses have reduced replication kinetics and fitness compared to the wild-type virus. Importantly, they show attenuation in both transgenic mice and golden Syrian hamsters, providing protection against SARS-CoV-2 infection and transmission. Vaccination with these attenuated viruses induces strong immune responses and can prevent COVID-19.
MICROBIOLOGY SPECTRUM
(2023)
Article
Veterinary Sciences
Yaru Mu, Quan Xie, Weikang Wang, Hao Lu, Mingjun Lian, Wei Gao, Tuofan Li, Zhimin Wan, Hongxia Shao, Aijian Qin, Jianqiang Ye
Summary: A novel live-attenuated vaccine candidate for FAdV-4, FAdV4-RFP_F1, was developed in this study and demonstrated high stability and efficient protection in both in vitro and in vivo experiments, offering new hope for the control and prevention of FAdV-4.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Virology
Giuditta De Lorenzo, Rapeepat Tandavanitj, Jennifer Doig, Chayanee Setthapramote, Monica Poggianella, Ricardo Sanchez-Velazquez, Hannah E. Scales, Julia M. Edgar, Alain Kohl, James Brewer, Oscar R. Burrone, Arvind H. Patel
Summary: The study developed ZIKV subunit and virus-like particle (VLP)-based vaccines, which induced antibodies with a higher capacity to neutralize virus infection and protected animals from lethal challenge with both the African and Asian lineages of ZIKV. The engineered immunogens reduced the potential to induce antibody-dependent enhancement (ADE) of infection and showed promising efficacy in comparison with the wild-type derivatives.
JOURNAL OF VIROLOGY
(2021)
Article
Immunology
Stephen J. Dollery, Daniel Zurawski, Elena K. Gaidamakova, Vera Y. Matrosova, John K. Tobin, Taralyn J. Wiggins, Ruth Bushnell, David A. MacLeod, Yonas A. Alamneh, Rania Abu-Taleb, Mariel G. Escatte, Heather N. Meeks, Michael J. Daly, Gregory J. Tobin
Summary: The study successfully produced new vaccines by utilizing A. baumannii cells cultured using various methods, and then treated with radiation and antioxidants, which significantly improved protection in mice.
Article
Immunology
Michelle Hui Pheng Lee, Chee Wah Tan, Han Kang Tee, Kien Chai Ong, I-Ching Sam, Yoke Fun Chan
Summary: The study aimed to develop a live-attenuated vaccine for EV-A71 by codon deoptimization and codon-pair deoptimization. The CD X and CPD X vaccine candidates elicited good neutralizing antibodies, conferred immune protection, making them promising candidates for EV-A71.
Article
Engineering, Biomedical
Si Sun, Entao Li, Gan Zhao, Jie Tang, Qianfei Zuo, Larry Cai, Chuanfei Xu, Cheng Sui, Yangxue Ou, Chang Liu, Haibo Li, Yuan Ding, Chao Li, Dongshui Lu, Weijun Zhang, Ping Luo, Ping Cheng, Yuwei Gao, Changchun Tu, Bruno Pitard, Joseph Rosenecker, Bin Wang, Yan Liu, Quanming Zou, Shan Guan
Summary: This study used self-assembled peptide-poloxamine nanoparticles for pulmonary inoculation of a COVID-19 DNA vaccine, which showed superior gene transfection and biocompatibility in the mouse airway. The vaccine induced mucosal immune responses against SARS-CoV-2, resulting in complete elimination of the infection and 100% survival rate in mice.
Article
Biochemistry & Molecular Biology
Karen A. Kirby, Yee Tsuey Ong, Atsuko Hachiya, Thomas G. Laughlin, Leslie A. Chiang, Yun Pan, Jennifer L. Moran, Bruno Marchand, Kamalendra Singh, Fabio Gallazzi, Thomas P. Quinn, Kazuhisa Yoshimura, Toshio Murakami, Shuzo Matsushita, Stefan G. Sarafianos
Article
Biochemical Research Methods
Elizabeth A. Dietrich, Richard A. Bowen, Aaron C. Brault
JOURNAL OF VIROLOGICAL METHODS
(2015)
Article
Microbiology
Nathan D. Grubaugh, Darci R. Smith, Doug E. Brackney, Angela M. Bosco-Lauth, Joseph R. Fauver, Corey L. Campbell, Todd A. Felix, Hannah Romo, Nisha K. Duggal, Elizabeth A. Dietrich, Tyler Eike, Jennifer E. Beane, Richard A. Bowen, William C. Black, Aaron C. Brault, Gregory D. Ebel
Article
Immunology
Terza Brostoff, Patricia A. Pesavento, Christopher M. Barker, Joan L. Kenney, Elizabeth A. Dietrich, Nisha K. Duggal, Angela M. Bosco-Lauth, Aaron C. Brault
Article
Infectious Diseases
Elizabeth A. Dietrich, Stanley A. Langevin, Claire Y. -H. Huang, Payal D. Maharaj, Mark J. Delorey, Richard A. Bowen, Richard M. Kinney, Aaron C. Brault
PLOS NEGLECTED TROPICAL DISEASES
(2016)
Editorial Material
Public, Environmental & Occupational Health
Natalie A. Kwit, Elizabeth A. Dietrich, Christina Nelson, Richard Taffner, Jeannine Petersen, Martin Schriefer, Paul Mead, Susan Weinstein, Dirk Haselow
MMWR-MORBIDITY AND MORTALITY WEEKLY REPORT
(2017)
Article
Microbiology
Andrew D. Huber, Eleftherios Michailidis, Megan L. Schultz, Yee T. Ong, Nicolin Bloch, Maritza N. Puray-Chavez, Maxwell D. Leslie, Juan Ji, Anthony D. Lucas, Karen A. Kirby, Nathaniel R. Landau, Stefan G. Sarafianos
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2014)
Article
Biochemistry & Molecular Biology
Eleftherios Michailidis, Andrew D. Huber, Emily M. Ryan, Yee T. Ong, Maxwell D. Leslie, Kayla B. Matzek, Kamalendra Singh, Bruno Marchand, Ariel N. Hagedorn, Karen A. Kirby, Lisa C. Rohan, Eiichi N. Kodama, Hiroaki Mitsuya, Michael A. Parniak, Stefan G. Sarafianos
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Article
Microbiology
Elizabeth A. Dietrich, Adam J. Replogle, Sarah W. Sheldon, Jeannine M. Petersen
Summary: The development of a real-time semimultiplex PCR assay has improved the diagnosis and classification of relapsing fever (RF) by detecting multiple RF borreliae and aiding in the clinical management of RF patients. This single-assay approach accurately detects and classifies RF borreliae, with high specificity and sensitivity.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Microbiology
Amanda E. Calvert, Kalanthe Horiuchi, Karen L. Boroughs, Yee T. Ong, Kimberly M. Anderson, Brad J. Biggerstaff, Mars Stone, Graham Simmons, Michael P. Busch, Claire Y-H Huang
Summary: The study demonstrates the persistence of IgM neutralizing antibodies after ZIKV infection, with IgM playing a predominant role in ZIKV neutralization and showing lower cross-reactivity compared to IgG.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Virology
Maria E. Cilento, Yee Tsuey Ong, Philip R. Tedbury, Stefan G. Sarafianos
Summary: Long-acting anti-HIV regimens have the potential to improve patients' quality of life by increasing dosing intervals. The combination therapy of lenacapavir with islatravir, rilpivirine, or cabotegravir shows additive inhibition in an HIV-1 reporter cell line. This study emphasizes the importance of effective drug combinations in long-acting regimens.
Article
Public, Environmental & Occupational Health
Elizabeth A. Dietrich, Yee Tsuey Ong, Janae L. Stovall, Hansi Dean, Claire Y. -H. Huang
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2017)
