Article
Fisheries
Ming-Guang Mao, Jia Xu, Rui-Ting Liu, Lin Ye, Rui Wang, Jie-lan Jiang
Summary: The Fas/FasL pathway in Pacific cod plays a crucial role in virus defense and cell apoptosis, with PcFas and PcFasL regulating EPC cell apoptosis by modulating the expression of multiple genes.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Anatomy & Morphology
Zhengguang Wang, Yongfang Tan, Fei Xu
Summary: This study aimed to investigate the effects of zonisamide (ZNS) on a degenerative cervical myelopathy (DCM) rat model and explore the potential mechanisms. The results showed that ZNS promoted motor recovery, reversed histopathological injury and cell apoptosis in DCM rats. Additionally, ZNS attenuated DCM injury through the regulation of Fas and FasL signaling. Therefore, this study provided a new theoretical approach for subsequent academic and clinical research on DCM injury.
Article
Cell Biology
Francesca Cuomo, Carmela Dell'Aversana, Teresa Chioccarelli, Veronica Porreca, Francesco Manfrevola, Chiara Papulino, Vincenzo Carafa, Rosaria Benedetti, Lucia Altucci, Gilda Cobellis, Gilda Cobellis
Summary: Maintaining energy balance is crucial for human health. This study highlights the role of Hif3 alpha in the transformation of white adipocytes to brown adipocytes, which activates beneficial thermogenesis and rewires energy metabolism. These findings have important implications for combating obesity and type 2 diabetes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Environmental Sciences
Fan Yu, Lianqi Yan, Jian Sun, Yumeng Zhao, Yan Yuan, Jianhong Gu, Jianchun Bian, Hui Zou, Zongping Liu
Summary: This study found that gap junction inhibitor may play a dual role in Cd-induced cell damage by inhibiting the transmission of death signals from damaged cells to healthy cells but also aggravating the transmission of death signals between damaged cells. The Fas/FasL-mediated death receptor pathway may play an important role in this process.
ENVIRONMENTAL TOXICOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Noor Mustafa, Jone Mitxelena, Arantza Infante, Olatz Zenarruzabeitia, Ainhoa Eriz, Ainhoa Iglesias-Ara, Ana M. Zubiaga
Summary: Disruption of E2f2 in mice leads to T-cell hyperactivation and disproportionate cell cycle entry. However, E2f2(-/-) mice do not develop lymphoproliferative condition. We find that E2f2 plays an anti-apoptotic role through the Fas pathway in vitro and in vivo. Knockout of E2f2 in TCR-stimulated T cells results in overexpression of proapoptotic genes, enhanced apoptosis, and activated p53 pathway. However, E2f2-Fas/FasL signaling is p53-independent. E2f2 is recruited to Fas and FasL gene promoters to repress their expression. E2f2(-/-) mice are prone to immune-mediated liver injury due to aberrant lymphoid Fas/FasL activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Hayley Muendlein, Wilson M. Connolly, Zoie Magri, Irina Smirnova, Vladimir Ilyukha, Avishekh Gautam, Alexei Degterev, Alexander Poltorak
Summary: In this study, the TRIF-dependent complex plays a crucial role in cell death and inflammation response, particularly in the context of bacterial infection. The constitutive binding between ZBP1 and RIPK1 is essential for TRIFosome interactions, caspase-8-mediated cell death, and inflammasome activation. This research provides an alternative model of pro-death complex formation reliant on TRIF signaling, demonstrating a non-canonical cell death pathway controlled by a complex of FADD, RIPK1, caspase-8, and ZBP1.
NATURE COMMUNICATIONS
(2021)
Article
Toxicology
Xue Fan, Yangping Zhu, Shuang Peng, Yingying Miao, Qian Lu, Luyong Zhang, Zhenzhou Jiang, Qinwei Yu
Summary: This study found that triptolide (TP) enhances the sensitivity of liver cells to the Fas/FasL pathway by inhibiting the expression of anti-apoptotic protein XIAP, leading to apoptosis of liver cells. This is of great significance for explaining the hepatotoxic mechanism of TP.
TOXICOLOGY LETTERS
(2023)
Article
Pharmacology & Pharmacy
Zhi-Yi Yan, Hai-Yan Jiao, Jian-Bei Chen, Kai-Wen Zhang, Xi-Hong Wang, You-Ming Jiang, Yue-Yun Liu, Zhe Xue, Qing-Yu Ma, Xiao-Juan Li, Jia-Xu Chen
Summary: This study explored the potential mechanism of Xiaoyaosan in treating depression through network pharmacology and experimental research. The results showed that Xiaoyaosan could regulate key target genes related to depression, improve depressive behaviors in mice, and alleviate neuroinflammation in the hippocampus caused by necroptosis, suggesting necroptosis as a potential new target for depression treatment.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Birte Niemann, Saskia Haufs-Brusberg, Laura Puetz, Martin Feickert, Michelle Y. Jaeckstein, Anne Hoffmann, Jelena Zurkovic, Markus Heine, Eva-Maria Trautmann, Christa E. Mueller, Anke Toenjes, Christian Schlein, Azin Jafari, Holger K. Eltzschig, Thorsten Gnad, Matthias Bluher, Natalie Krahmer, Peter Kovacs, Joerg Heeren, Alexander Pfeifer
Summary: This study reveals that inosine, a metabolite released during apoptosis, enhances the thermogenic program in healthy adipocytes and regulates energy expenditure. The equilibrative nucleoside transporter 1 (ENT1) controls inosine levels in brown adipose tissue, affecting thermogenic capacity. In humans, a loss of function mutation in ENT1 is associated with lower body mass index and reduced odds of obesity.
Article
Chemistry, Medicinal
Hui Li, Ting-Jun Fan, Ping Zou, Bin Xu
Summary: The study evaluated the cytotoxicity of DFS on human corneal epithelial cells and found that it caused abnormal cell morphology and decreased cell viability. DFS induced ROS-mediated DNA damage, activated the p53-dependent apoptosis pathway, disrupted mitochondrial membrane potential, and led to cytochrome c release into the cytoplasm.
CHEMICAL RESEARCH IN TOXICOLOGY
(2021)
Article
Pharmacology & Pharmacy
Wenchang Yang, Kaixiong Tao, Yaxin Wang, Yongzhou Huang, Caihan Duan, Tao Wang, Chengguo Li, Peng Zhang, Yuping Yin, Jinbo Gao, Ruidong Li
Summary: Inflammatory bowel disease (IBD) is a chronic disorder of the gastrointestinal tract with insufficient therapeutic efficacy. Recent research has shown that necrosulfonamide (NSA) can protect against various inflammatory diseases by blocking the polymerization of mixed lineage kinase domain-like protein (MLKL). This study demonstrates that NSA can alleviate symptoms of dextran sodium sulfate (DSS)-induced colitis in mouse models by reducing weight loss and disease activity. NSA also inhibits the accumulation of macrophages and CD4+/CD8+ T-cells in the colon tissue caused by DSS. Mechanistically, NSA inhibits pyroptosis and necroptosis pathways by reducing the expression of phosphorylated MLKL and N-gasdermin D (N-GSDMD).
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Cell Biology
Hongli Zhou, Minyu Zhou, Yue Hu, Yanin Limpanon, Yubin Ma, Ping Huang, Paron Dekumyoy, Wanchai Maleewong, Zhiyue Lv
Summary: The study revealed that TNF-α plays a central role in inducing apoptosis and necroptosis in the context of Angiostrongylus cantonensis (AC) infection, with astrocytes undergoing apoptosis through the RIP1/FADD/Caspase-8 axis and neurons undergoing necroptosis through the RIP3/MLKL pathway. In addition, it was shown that TNF-α secretion by microglia upon AC stimulation caused necroptosis of neurons. This research provides new insight into targeting TNF-α signaling as a therapeutic strategy for central nervous system injuries caused by AC infection.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2022)
Article
Toxicology
Hongna Sun, Yanmei Yang, Muyu Gu, Yang Li, Zhe Jiao, Chunqing Lu, Bingyu Li, Yuting Jiang, Lixin Jiang, Fang Chu, Wenjing Yang, Dianjun Sun, Yanhui Gao
Summary: This study found that arsenic induces neurotoxicity by activating the Fas-FasL-FADD signaling pathway and inducing neuronal apoptosis.
TOXICOLOGY LETTERS
(2022)
Article
Medicine, Research & Experimental
Yasmin S. Abulfadl, Yousef Abo El Ela, Abdallah M. Al Khaiyat, Khalil I. Elkhodary, Mohamed Badran
Summary: This study examines the negative impact of CP on cardiac contractility, both in vitro and in vivo, and reveals the underlying mechanism through which CP induces myocardial insult. Results show that CP alters heart, body, and heart-to-body weight, disrupts cardiac muscle architecture, increases oxidative stressors, and decreases antioxidant status. Mechanistically, CP prompts the necroptotic trajectory by activating certain proteins and inhibiting others, leading to impaired myocardial contractility. This study identifies necroptosis as a potential target for mitigating CP-induced cardiac toxicity.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Tapasi Roy, Abhishek Chatterjee, Snehasikta Swarnakar
Summary: Rotenone has both beneficial effects in agriculture, fisheries and animal husbandries, as well as detrimental effects on the health of personnel in these industries. Prolonged exposure to rotenone can lead to neurodegeneration and the development of Parkinson's disease symptoms. Our study showed that rotenone exposure in N2A cells results in increased ROS production, decreased ATP levels, and loss of mitochondrial membrane potential. The expression of cofilin, a key component of actin cytoskeleton, was altered and the actin cytoskeleton degraded. MMP9 expression and activity increased, indicating inflammation upon rotenone exposure. TNF-alpha levels and necroptosis markers also increased, suggesting programmed necroptotic death.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Shohei Yoshimoto, Miho Matsuda, Kenichi Kato, Eijiro Jimi, Hiroshi Takeuchi, Shuji Nakano, Shunichi Kajioka, Etsuko Matsuzaki, Takao Hirofuji, Ryuji Inoue, Masato Hirata, Hiromitsu Morita
Summary: The study found that VRAC is highly functional and associated with proliferation in HST-1 cells, but not in HaCaT cells. The pharmacological profile of VRAC in HST-1 was similar to previous reports, and inhibition of VRAC with DCPIB led to apoptosis in HST-1 cells. Knockdown of TMEM16A and LRRC8A attenuated VRAC activity in HST-1 cells.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Satoru Mukai, Akiko Mizokami, Takahito Otani, Tomomi Sano, Miho Matsuda, Sakura Chishaki, Jing Gao, Tomoyo Kawakubo-Yasukochi, Ronghao Tang, Takashi Kanematsu, Hiroshi Takeuchi, Eijiro Jimi, Masato Hirata
Summary: GPRC6A regulates various physiological processes in response to its interaction with multiple ligands and its constitutive activation in adipocytes plays a key role in adipose lipid handling and the prevention of obesity and related metabolic disorders.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Cell Biology
Jing Gao, Akiko Mizokami, Hiroshi Takeuchi, Aonan Li, Fei Huang, Haruki Nagano, Takashi Kanematsu, Eijiro Jimi, Masato Hirata
Summary: PRIP facilitates insulin signaling by modulating the internalization of insulin receptor (IR) in adipocytes, playing an important regulatory role in insulin signaling.
JOURNAL OF CELL SCIENCE
(2022)
Article
Microscopy
Shoko Ishikawa, Misaki Nikaido, Takahito Otani, Kayoko Ogata, Hiroshi Iida, Yuko Inai, Sachio Tamaoki, Tetsuichiro Inai
Summary: This study investigated the effects of RXR antagonist HX 531 on K38 cell 3D cultures and found that it improved cell morphology and intercellular junctions. The treated cells exhibited thinner morphology and narrower intercellular spaces, with increased levels of desmosomal proteins and tight junction proteins.
Article
Anatomy & Morphology
Norio Kitagawa
Summary: This study investigates the effects of Lactococcus lactis on anti-aging and reveals that the antimicrobial peptide Nisin alters the distribution of intermediate filament proteins CK5 and CK17 in human keratinocytes. The study also suggests that CK5 and CK17 in a spherical distribution may be involved in a macropinosome-like structure. These findings provide new insights into the mechanism of action of Nisin and its potential anti-aging effects.
ANATOMY & CELL BIOLOGY
(2022)
Article
Anatomy & Morphology
Joe Iwanaga, Nicholas Gerard, Basem Ishak, Lukasz Olewnik, George P. Georgiev, Anand P. Patel, Norio Kitagawa, Aaron S. Dumont, R. Shane Tubbs
Summary: This is an anatomical study on the interosseous crural nerve (IOCn), which found that the nerve has vascular, muscular, and ligamentous branches. This is of clinical relevance for surgeons operating in the deep posterior compartment of the leg and ankle, as well as clinicians treating patients with unusual presentations or pathology of the leg and ankle.
Article
Endocrinology & Metabolism
Kayo Mori, Akiko Mizokami, Tomomi Sano, Satoru Mukai, Fumitaka Hiura, Yasunori Ayukawa, Kiyoshi Koyano, Takashi Kanematsu, Eijiro Jimi
Summary: The study suggests that activation of the non-canonical NF-kappa B pathway through elevation of serum RANKL levels contributes to postmenopausal obesity.
JOURNAL OF ENDOCRINOLOGY
(2022)
Article
Anatomy & Morphology
Norio Kitagawa, Joe Iwanaga, R. Shane Tubbs, Hongtae Kim, Yong-Suk Moon, Mi-Sun Hur
Summary: This study reports rare connecting fibers between the orbicularis oculi and orbicularis oris muscles. These fibers blend with adjacent muscles and their contractions can affect typical facial expressions.
ANATOMY & CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Fumitaka Hiura, Yuko Kawabata, Tsukasa Aoki, Akiko Mizokami, Eijiro Jimi
Summary: The study reveals the importance of ATG4/LC3 in osteoclast maturation and bone resorption. By using a specific inhibitor of ATG4B, NSC185058 (NSC), osteoclastogenesis and maturation can be inhibited, leading to a decrease in alveolar bone resorption in a periodontitis model.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Anatomy & Morphology
Joe Iwanaga, Puhan He, Keiko Fukino, Mi-Sun Hur, Hee-Jin Kim, Aya Han, Koichi Watanabe, Soichiro Ibaragi, Norio Kitagawa, R. Shane Tubbs
Summary: The anatomy of the superior labial frenulum involves a mucosal fold between the gingival mucosa and alveolar mucosa, which is associated with the incisivus labii superioris. Understanding the anatomical relationship between these structures is crucial in studying the superior labial frenulum.
Article
Anatomy & Morphology
Joe Iwanaga, Neal Jackson, Taku Toriumi, Ikuo Kageyama, Francisco Reina, Ana Carrera, Keiko Fukino, Norio Kitagawa, R. Shane Tubbs
Summary: This study aimed to clarify the anatomy and definition of the sublingual nerves. Through microsurgical dissection of cadaveric hemiheads, the sublingual nerves were found to have three branches, i.e., branches to the sublingual gland, branches to the mucosa of the floor of the mouth, and gingival branches. Additionally, branches to the sublingual gland were subcategorized into types I and II based on their origin.
Article
Biochemistry & Molecular Biology
Mineyuki Mizuguchi, Takeshi Yokoyama, Takahito Otani, Shiori Kuribara, Yuko Nabeshima, Takayuki Obita, Masato Hirata, Keiichi Kawano
Summary: An acidic environment in bone is crucial for bone metabolism and the production of decarboxylated osteocalcin. The crystal structure of decarboxylated osteocalcin at pH 2.0 showed that it retains the alpha-helix structure and is stable under acidic conditions. Additionally, the activity of decarboxylated osteocalcin in inducing adiponectin is related to specific mutations at Glu17 and Glu21.
Editorial Material
Dentistry, Oral Surgery & Medicine
Norio Kitagawa, Keiko Fukino, Joe Iwanaga
JOURNAL OF DENTAL EDUCATION
(2023)
Article
Dentistry, Oral Surgery & Medicine
Mayuka Yamagata, Sen Higashi, Noboru Yaida, Akane Ichihara, Manabu Habu, Masaaki Sasaguri, Kazuhiro Tominaga, Hiroshi Takeuchi
Summary: Metformin reduces the expression of EGFR in cancer cells and inhibits cell proliferation by promoting ligand-induced internalization and degradation of EGFR. This finding suggests that the effect of metformin on EGFR may impact therapeutic outcomes when combined with other cancer treatments.
ORAL SCIENCE INTERNATIONAL
(2023)
Article
Surgery
Kisho Ono, Soichiro Ibaragi, Kyoichi Obata, Tatsuo Okui, Norio Kitagawa, R. Shane Tubbs, Joe Iwanaga
Summary: In this study, we investigated the possibility of facial nerve reanimation during submental island flap procedures by preserving the mylohyoid muscle. Our results demonstrate that the nerve to the mylohyoid muscle can be used to reanimate the facial nerve in cases of intraoperative facial nerve injury.
JOURNAL OF CRANIOFACIAL SURGERY
(2023)