Article
Spectroscopy
Shijun Chen, Mengyao Liu, Yunjiang Zi, Junyi He, Lin Wang, Yuanyuan Wu, Shicong Hou, Wenxue Wu
Summary: Beta-galactosidase (beta-gal) is a hydrolytic enzyme in lysosomes and an important biomarker of cellular senescence and ovarian cancer. We designed an enzyme-activated near-infrared probe for real-time detection of beta-gal activity in vivo. The probe has short response time, large Stokes displacement, and near-infrared fluorescence emission, and has been successfully used to detect beta-gal in cancer cells and senile cells.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Xiao-Peng Fan, Wen Yang, Tian-Bing Ren, Shuai Xu, Xiang-Yang Gong, Xiao-Bing Zhang, Lin Yuan
Summary: In this study, an amphiphilic molecular probe was developed for in situ imaging of drug-induced liver injury. The probe showed selectivity and sensitivity towards the target compound and could be used to assess liver injury and analyze the therapeutic efficacy of hepatoprotective medicines.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Yongliang Chen, Chi Li, Tieshan Yang, Evgeny A. Ekimov, Carlo Bradac, Son Tung Ha, Milos Toth, Igor Aharonovich, Toan Trong Tran
Summary: All-optical nanothermometry is a powerful tool for measuring nanoscale temperatures in various applications. This study presents a real-time nanothermometry technique using codoped nanodiamonds with high sensitivity and resolution. The technique utilizes temperature sensors that emit spectrally separated fluorescence signals and a parallel detection scheme for fast readout. The method is demonstrated by monitoring temperature changes in microcircuits and MoTe2 field-effect transistors.
Article
Chemistry, Analytical
Xiaowan Han, Xiangchuan Meng, Xiang Wang, Shan Leng, Qian Liu, Leilei Zhang, Pingping Li, Qingyang Zhang, Hai-Yu Hu
Summary: A new excited-state intramolecular proton transfer-delayed fluorescence (ESIPT-DF) approach was reported to construct analyte-stimulated DF probes. These new classes of ESIPT-DF luminophores, including HBT-PXZ and HBT-PTZ, exhibited significant ESIPT and DF characters. Furthermore, a caged probe HBT-PXZ-Ga was developed and applied to investigate the influence of metal ions on beta-gal activity.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Hui Dong, Le Zhao, Xu Zhu, Xiuhua Wei, Menghui Zhu, Qingmin Ji, Xueke Luo, Yintang Zhang, Yanli Zhou, Maotian Xu
Summary: In this study, a turn-on ratiometric electrochemical sensor was fabricated for the detection of beta-Galactosidase. The sensor showed rapid, simple, accurate, and highly sensitive detection with a wide linear range and low detection limit.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Yingcui Bu, Haoran Wang, Yu Deng, Feng Zhong, Zhi-peng Yu, Xiaojiao Zhu, Hongping Zhou
Summary: In this study, a photo-activated fluorescent probe (CQ-IC) was designed and prepared to analyze and monitor the microenvironmental homeostasis of the plasma membrane (PM) based on a two-channel ratiometric imaging model. Upon light irradiation, CQ-IC generates reactive oxygen species and increases cellular viscosity, resulting in enhanced emission peaks at 480 and 610 nm. This research proposes a new strategy to sense PM homeostasis and effectively guide the treatment of viscosity-related diseases in various physiological and pathological processes.
ANALYTICAL CHEMISTRY
(2023)
Article
Materials Science, Multidisciplinary
Yunyi Liu, Lu Zhang, Ying Chen, Haitao Sun, Jiajian Chen, Ahmed Mohamed Ei-Toni, Aslam Khan, Zuhai Lei, Fan Zhang
Summary: ATP plays a crucial role in immune cell responses and its real-time monitoring in vivo remains challenging. In this study, a reversible NIR-II molecular fluorescent probe (CX-RATP) based on FRET strategy is designed and synthesized. CX-RATP exhibits significant fluorescence ratio change in the presence of physiological and pathological ATP concentrations. The recognition process can be reversed by apyrase and the probe is successfully applied in detecting ATP levels in acute inflammation and hepatocellular carcinoma.
ADVANCED OPTICAL MATERIALS
(2023)
Article
Chemistry, Analytical
Yu-An Feng, Hai Xu, Yang Zhou, Bao-Jun Wang, Jian Xiao, Ya-Wen Wang, Yu Peng
Summary: The newly designed NIR fluorescent probe SWJT-3 is capable of ratiometric determination of alkaline phosphatase (ALP) and can be used for both in vitro and in vivo detection. It is the first NIR and ratiometric fluorescent probe for imaging ALP in mice, with a large Stokes shift and low detection limit.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Spectroscopy
Dan Liu, Zixuan Zhang, Anying Chen, Peng Zhang
Summary: Fluorescent probes based on chemical reactions are widely studied for disease diagnosis, with HBT-gal being a probe developed for detecting beta-gal activity, showing high selectivity and sensitivity for imaging cell senescence.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Biotechnology & Applied Microbiology
Weiwang Dou, Quanchao Zhu, Meihua Zhang, Zuyuan Jia, Wenjun Guan
Summary: This study identified 8 strong endogenous promoters in P. pastoris using RNA-seq and LacZ reporter system, which contributed to higher transcriptional expression levels and beta-galactosidase activities. These promoters were applied to a heterologous beta-carotene biosynthetic pathway, resulting in the highest yield of beta-carotene up to 1.07 mg/g. Simultaneously using the same promoter multiple times may lead to a notable competitive effect and lower the transcriptional expression level of the target gene.
MICROBIAL CELL FACTORIES
(2021)
Article
Nanoscience & Nanotechnology
Chun Wu, Ping Tan, Xianjin Chen, Hongrong Chang, Yuhui Chen, Gehong Su, Tao Liu, Zhiwei Lu, Mengmeng Sun, Yanying Wang, Yuanfeng Zou, Jian Wang, Hanbing Rao
Summary: In this study, a spermine-responsive triple-emission ratiometric fluorescent probe was designed and fabricated using dual-emissive carbon nanoparticles and quantum dots. The probe showed improved accuracy and reduced interference from the environment. It demonstrated good emission resolution and proportional detection of spermine, allowing real-time, visual, and high-throughput detection of seafood and meat freshness. The reaction mechanism was verified using density functional theory and fundamental characterizations.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemical Research Methods
Neha Irfan, Neha Soleja, Mohd Mohsin
Summary: Inadequate folic acid intake is associated with various diseases, but traditional methods of determining folic acid levels are unreliable and time-consuming. We have developed SenFol, a highly specific and quick-responding nanosensor, which shows potential for dynamic detection of intracellular folic acid content in different cells.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Spectroscopy
Xiaoqian Huang, Xiangzhu Chen, Shijun Chen, Xueyan Zhang, Lin Wang, Shicong Hou, Xiaodong Ma
Summary: A novel ratiometric fluorescent probe has been developed for the detection of alkaline phosphatase (ALP) and fluorescence imaging in living cells, showing high accuracy and sensitivity. The probe exhibits a significant fluorescence color change from blue to green in response to ALP, with a detection limit as low as 0.16 U L-1. Additionally, the probe demonstrates excellent biocompatibility for application in biological systems.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Cell Biology
Tom Zimmermann, Michaela Pommer, Viola Kluge, Chafia Chiheb, Susanne Muehlich, Anja-Katrin Bosserhoff
Summary: Detection and quantification of senescent cells is challenging. It is accepted to use a combination of markers and cellular characteristics to define senescent cells in vitro. This study provides a comprehensive comparison of biomarkers and cellular characteristics used to detect senescence in melanocytic systems, aiming to support the development of standardized senescence detection and quantification.
Article
Chemistry, Multidisciplinary
Franco F. Faucher, Kevin J. Liu, Emily D. Cosco, John C. Widen, Jonathan Sorger, Matteo Guerra, Matthew Bogyo
Summary: Surgery is the preferred treatment for solid tumors, but inaccurate cancer border detection can result in incomplete removal or excessive excision of tissue. We describe a strategy to convert fluorescent probes into ratiometric contrast agents, improving tumor detection sensitivity. We also built a camera system for real-time imaging of ratiometric signals during surgery, showing the potential for clinical implementation to improve cancer resection.
ACS CENTRAL SCIENCE
(2023)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).