In-Cell NMR Spectroscopy of Intrinsically Disordered Proteins

Potent Inhibitors of Mycobacterium tuberculosis Growth Identified by Using in-Cell NMR-based Screening

(2018) Christopher M. DeMott et al.   ACS Chemical Biology

Real-Time In-Cell Nuclear Magnetic Resonance: Ribosome-Targeted Antibiotics Modulate Quinary Protein Interactions

(2018) Leonard Breindel et al.   BIOCHEMISTRY

3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy

(2016) Bin-Bin Pan et al.   CHEMICAL COMMUNICATIONS

New insights into cellular α-synuclein homeostasis in health and disease

(2016) Ulf Dettmer et al.   CURRENT OPINION IN NEUROBIOLOGY

In-Cell Protein Structures from 2D NMR Experiments

(2016) Thomas Müntener et al.   Journal of Physical Chemistry Letters

Structural disorder of monomeric α-synuclein persists in mammalian cells

(2016) Francois-Xavier Theillet et al.   NATURE

Characterization of proteins by in-cell NMR spectroscopy in cultured mammalian cells

(2016) Letizia Barbieri et al.   Nature Protocols

Small molecules, big targets: drug discovery faces the protein–protein interaction challenge

(2016) Duncan E. Scott et al.   NATURE REVIEWS DRUG DISCOVERY

Real-Time Monitoring of Cancer Cell Metabolism and Effects of an Anticancer Agent using 2D In-Cell NMR Spectroscopy

(2015) He Wen et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Probing Protein Quinary Interactions by In-Cell Nuclear Magnetic Resonance Spectroscopy

(2015) Subhabrata Majumder et al.   BIOCHEMISTRY

Hydrogen exchange of disordered proteins inEscherichia coli

(2015) Austin E. Smith et al.   PROTEIN SCIENCE

Caught in Action: Selecting Peptide Aptamers Against Intrinsically Disordered Proteins in Live Cells

(2015) Jacqueline D. Cobbert et al.   Scientific Reports

The Synaptic Function of α-Synuclein

(2015) Jacqueline Burré   Journal of Parkinsons Disease

The molecular mechanism of nuclear transport revealed by atomic-scale measurements

(2015) Loren E Hough et al.   eLife

Real-Time Monitoring of New Delhi Metallo-β-Lactamase Activity in Living Bacterial Cells by1H NMR Spectroscopy

(2014) Junhe Ma et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Using Singular Value Decomposition to Characterize Protein-Protein Interactions by In-cell NMR Spectroscopy

(2014) Subhabrata Majumder et al.   CHEMBIOCHEM

Residue level quantification of protein stability in living cells

(2014) W. B. Monteith et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

19F NMR Spectroscopy as a Probe of Cytoplasmic Viscosity and Weak Protein Interactions in Living Cells

(2013) Yansheng Ye et al.   CHEMISTRY-A EUROPEAN JOURNAL

Atomic-resolution monitoring of protein maturation in live human cells by NMR

(2013) Lucia Banci et al.   Nature Chemical Biology

In-Cell NMR Characterization of the Secondary Structure Populations of a Disordered Conformation of α-Synuclein within E. coli Cells

(2013) Christopher A. Waudby et al.   PLoS One

Fate of Pup inside the Mycobacterium Proteasome Studied by in-Cell NMR

(2013) Andres Y. Maldonado et al.   PLoS One

Impact of reconstituted cytosol on protein stability

(2013) M. Sarkar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Gel-Encapsulated Bioreactor System for NMR Studies of Protein-Protein Interactions in Living Mammalian Cells

(2012) Satoshi Kubo et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Bacterial in-cell NMR of human α-synuclein: a disordered monomer by nature?: Figure 1

(2012) Andres Binolfi et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Determination of Secondary Structure Populations in Disordered States of Proteins Using Nuclear Magnetic Resonance Chemical Shifts

(2012) Carlo Camilloni et al.   BIOCHEMISTRY

The Permeability of Reconstituted Nuclear Pores Provides Direct Evidence for the Selective Phase Model

(2012) Bastian B. Hülsmann et al.   CELL

Direct Observation of the Interconversion of Normal and Toxic Forms of α-Synuclein

(2012) Nunilo Cremades et al.   CELL

Characterization of Semisynthetic and NaturallyNα-Acetylated α-Synucleinin Vitroand in Intact Cells

(2012) Bruno Fauvet et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure of Proteins in Eukaryotic Compartments

(2012) Karl Bertrand et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Rapid Distinction of Intracellular and Extracellular Proteins Using NMR Diffusion Measurements

(2012) Christopher A. Waudby et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Interaction of α-Synuclein and a Cell Penetrating Fusion Peptide with Higher Eukaryotic Cell Membranes Assessed by 19F NMR

(2012) Imola G. Zigoneanu et al.   MOLECULAR PHARMACEUTICS

N-terminal acetylation is critical for forming α-helical oligomer of α-synuclein

(2012) Adam J. Trexler et al.   PROTEIN SCIENCE

13C Direct-Detection Biomolecular NMR Spectroscopy in Living Cells

(2011) Ivano Bertini et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

α-Synuclein occurs physiologically as a helically folded tetramer that resists aggregation

(2011) Tim Bartels et al.   NATURE

Identification of Novel α-Synuclein Isoforms in Human Brain Tissue by using an Online NanoLC-ESI-FTICR-MS Method

(2011) Annika Öhrfelt et al.   NEUROCHEMICAL RESEARCH

A soluble  -synuclein construct forms a dynamic tetramer

(2011) W. Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Internal and Global Protein Motion Assessed with a Fusion Construct and In-Cell NMR Spectroscopy

(2010) Christopher O. Barnes et al.   CHEMBIOCHEM

The mycobacterial Mpa–proteasome unfolds and degrades pupylated substrates by engaging Pup's N-terminus

(2010) Frank Striebel et al.   EMBO JOURNAL

Prokaryotic Ubiquitin-Like Protein Provides a Two-Part Degron to Mycobacterium Proteasome Substrates

(2010) K. E. Burns et al.   JOURNAL OF BACTERIOLOGY

15NH/D-SOLEXSY experiment for accurate measurement of amide solvent exchange rates: application to denatured drkN SH3

(2010) Veniamin Chevelkov et al.   JOURNAL OF BIOMOLECULAR NMR

On the measurement of 15N–{1H} nuclear Overhauser effects. 2. Effects of the saturation scheme and water signal suppression

(2010) Fabien Ferrage et al.   JOURNAL OF MAGNETIC RESONANCE

A Bimodal Distribution of Two Distinct Categories of Intrinsically Disordered Structures with Separate Functions in FG Nucleoporins

(2010) Justin Yamada et al.   MOLECULAR & CELLULAR PROTEOMICS

Binding-induced folding of prokaryotic ubiquitin-like protein on the Mycobacterium proteasomal ATPase targets substrates for degradation

(2010) Tao Wang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Targeted Amino-Terminal Acetylation of Recombinant Proteins in E. coli

(2010) Matthew Johnson et al.   PLoS One

19F NMR studies of α-synuclein-membrane interactions

(2010) Gui-Fang Wang et al.   PROTEIN SCIENCE

In-cell protein NMR and protein leakage

(2010) Christopher O. Barnes et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Pup, a prokaryotic ubiquitin-like protein, is an intrinsically disordered protein

(2009) Shanhui Liao et al.   BIOCHEMICAL JOURNAL

19F NMR Studies of α-Synuclein Conformation and Fibrillation

(2009) Conggang Li et al.   BIOCHEMISTRY

Theory, Practice, and Applications of Paramagnetic Relaxation Enhancement for the Characterization of Transient Low-Population States of Biological Macromolecules and Their Complexes

(2009) G. Marius Clore et al.   CHEMICAL REVIEWS

A distinct structural region of the prokaryotic ubiquitin-like protein (Pup) is recognized by the N-terminal domain of the proteasomal ATPase Mpa

(2009) Markus Sutter et al.   FEBS LETTERS

H-start for exclusively heteronuclear NMR spectroscopy: The case of intrinsically disordered proteins

(2009) Wolfgang Bermel et al.   JOURNAL OF MAGNETIC RESONANCE

A bioreactor for in-cell protein NMR

(2009) Naima G. Sharaf et al.   JOURNAL OF MAGNETIC RESONANCE

Screening of Small Molecule Interactor Library by Using In-Cell NMR Spectroscopy (SMILI-NMR)

(2009) Jingjing Xie et al.   JOURNAL OF MEDICINAL CHEMISTRY

Prokaryotic Ubiquitin-Like Protein Pup Is Intrinsically Disordered

(2009) Xiang Chen et al.   JOURNAL OF MOLECULAR BIOLOGY

Multiple Tight Phospholipid-Binding Modes of α-Synuclein Revealed by Solution NMR Spectroscopy

(2009) Christina R. Bodner et al.   JOURNAL OF MOLECULAR BIOLOGY

Speeding Up13C Direct Detection Biomolecular NMR Spectroscopy

(2009) Wolfgang Bermel et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Accurate Sampling of High-Frequency Motions in Proteins by Steady-State15N−{1H} Nuclear Overhauser Effect Measurements in the Presence of Cross-Correlated Relaxation

(2009) Fabien Ferrage et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Observation of NMR Signals from Proteins Introduced into Living Mammalian Cells by Reversible Membrane Permeabilization Using a Pore-Forming Toxin, Streptolysin O

(2009) Shinji Ogino et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Determination of the Free Energy Landscape of α-Synuclein Using Spin Label Nuclear Magnetic Resonance Measurements

(2009) Jane R. Allison et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Inhibitors selective for mycobacterial versus human proteasomes

(2009) Gang Lin et al.   NATURE

Protein structure determination in living cells by in-cell NMR spectroscopy

(2009) Daisuke Sakakibara et al.   NATURE

High-resolution multi-dimensional NMR spectroscopy of proteins in human cells

(2009) Kohsuke Inomata et al.   NATURE

Prokaryotic ubiquitin-like protein (Pup), proteasomes and pathogenesis

(2009) K. Heran Darwin   NATURE REVIEWS MICROBIOLOGY

Structural Insights on the Mycobacterium tuberculosis Proteasomal ATPase Mpa

(2009) Tao Wang et al.   STRUCTURE

Proteasomal Protein Degradation in Mycobacteria Is Dependent upon a Prokaryotic Ubiquitin-like Protein

(2008) Kristin E. Burns et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Model-independent interpretation of NMR relaxation data for unfolded proteins: the acid-denatured state of ACBP

(2008) Kristofer Modig et al.   JOURNAL OF BIOMOLECULAR NMR

NMR observation of Tau in Xenopus oocytes

(2008) Jean-François Bodart et al.   JOURNAL OF MAGNETIC RESONANCE

Differential Dynamical Effects of Macromolecular Crowding on an Intrinsically Disordered Protein and a Globular Protein: Implications for In-Cell NMR Spectroscopy

(2008) Conggang Li et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Artificial nanopores that mimic the transport selectivity of the nuclear pore complex

(2008) Tijana Jovanovic-Talisman et al.   NATURE

Hydrogen exchange of monomeric α-synuclein shows unfolded structure persists at physiological temperature and is independent of molecular crowding inEscherichia coli

(2008) Robyn L. Croke et al.   PROTEIN SCIENCE

Ubiquitin-Like Protein Involved in the Proteasome Pathway of Mycobacterium tuberculosis

(2008) M. J. Pearce et al.   SCIENCE

Solvent-induced collapse of α-synuclein and acid-denatured cytochrome c

(2002) Artemiza S. Morar et al.   PROTEIN SCIENCE