期刊
ACTA CIRURGICA BRASILEIRA
卷 31, 期 3, 页码 176-182出版社
ACTA CIRURGICA BRASILEIRA
DOI: 10.1590/S0102-865020160030000005
关键词
Inflammation; Ischemia; Reperfusion; Allopurinol; Apoptosis; Rats
类别
资金
- National Natural Science Foundation of China [81400753]
- Hubei Natural Science Foundation [2014CFB362]
- bureau of public health of Hubei Province [JX6B14]
- project of Wuhan Municipal Science and Technology Bureau [2013062301010808]
PURPOSE: To investigate the potential effects of pretreatment with allopurinol on renal ischemia/reperfusion injury (IRI) in a rat model. METHODS: Twenty four rats were subjected to right kidney uninephrectomy were randomly distributed into the following three groups (n=8): Group A (sham-operated group); Group B (ischemic group) with 30 min of renal ischemia after surgery; and Group C (allopurinol + ischemia group) pretreated with allopurinol at 50 mg/kg for 14 days. At 72 h after renal reperfusion, the kidney was harvested to assess inflammation and apoptosis. RESULTS: Pretreatment with allopurinol significantly improved renal functional and histological grade scores following I/R injury (p< 0.05). Compared with Group B, the expression levels of caspase-3 and Bax were markedly reduced in Group C, meanwhile, whereas expression of bcl-2 was clearly increased (p< 0.05). A newly described marker of inflammation, High Mobility Group Box 1(HMGB1), showed reduced expression in Group C (p< 0.05). CONCLUSION: Pretreatment with allopurinol had a protective effect on kidney ischemia/reperfusion injury, which might be related to the inhibition of HMGB1 expression.
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