Article
Medicine, Research & Experimental
Rifa Widyaningrum, Thierry Burnouf, Ouada Nebie, Liling Delila, Tsung-Jen Wang
Summary: The study assessed the potential of using tailor-made HPL for treating age-related decrease in corneal endothelial cell numbers, and characterized the HPL using proteomics tools, revealing a richness in growth factors and antioxidants. Treatments with HPL showed increased cell viability, enhanced wound closure rate, and protection against oxidative stress, supporting further evaluation of HPL as a novel biotherapy for corneal endothelial regeneration.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Nanoscience & Nanotechnology
Johanka Taborska, Andreu Blanquer, Eduard Brynda, Elena Filova, Lenka Stiborova, Vera Jencova, Kristyna Havlickova, Zuzana Riedelova, Tomas Riedel
Summary: This study developed a bioactive wound dressing using a biodegradable nanofibrous blend of poly(L-lactide-co-epsilon-caprolactone) and poly(epsilon-caprolactone) (PLCL/PCL) covered by a thin fibrin layer for sustained release of bioactive molecules. The fibrin coating enriched with human platelet lysate (hPL), fibroblast growth factor 2 (FGF), and vascular endothelial growth factor (VEGF) showed both fast and sustained release of bioactive molecules, promoting the viability of human saphenous vein endothelial cells (HSVECs). The presence of alpha-2 antiplasmin stabilized the fibrin mesh and prevented its degradation.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Tong Lei, Yanyan Liu, Shiwen Deng, Zhuangzhuang Xiao, Yanjie Yang, Xiaoshuang Zhang, Wangyu Bi, Hongwu Du
Summary: This study found that hydrogel supplemented with HPL promotes SHED proliferation and contributes to their differentiation after induction. Furthermore, SHED encapsulated in the hydrogel also promotes migration and angiogenesis of HUVEC.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Materials Science, Biomaterials
Gang Liu, Boqing Zhang, Ting Wan, Changchun Zhou, Yujiang Fan, Weidong Tian, Wei Jing
Summary: In this study, a novel bioactive scaffold loaded with platelet lysate/gelatin methacrylate (PL/GelMA) in a 3D-printed biphasic calcium phosphate (BCP) scaffold was proposed to enhance scaffold vascularization. The PL/GelMA/BCP scaffold successfully promoted cell proliferation and the formation of blood vessel-like structures in vitro. Moreover, implantation of the scaffold in vivo also resulted in increased capillary formation. These findings suggest that the PL/GelMA/BCP scaffold has the potential to promote vascularization in tissue engineering.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Cell Biology
Antonella Bordin, Maila Chirivi, Francesca Pagano, Marika Milan, Marco Iuliano, Eleonora Scaccia, Orazio Fortunato, Giorgio Mangino, Xhulio Dhori, Elisabetta De Marinis, Alessandra D'Amico, Selenia Miglietta, Vittorio Picchio, Roberto Rizzi, Giovanna Romeo, Fabio Pulcinelli, Isotta Chimenti, Giacomo Frati, Elena De Falco
Summary: Platelet lysate (PL) contains a subset of small vesicles with angiogenic effects, enriched with miR-126. These extracellular vesicles (EVs) play a key role in promoting angiogenic tubular structures in endothelial cells, representing a potential target for developing a product solely made of EVs for biological manipulation.
CELL PROLIFERATION
(2022)
Article
Materials Science, Biomaterials
Richard da Costa Marques, Johanna Simon, Cyril D'Arros, Katharina Landfester, Kerstin Jurk, Volker Mailaender
Summary: This study investigated the adsorbed protein composition on calcium phosphate (CaP) surfaces combined with hemoderivatives, and found abundant non-regenerative and anti-angiogenic proteins on the surfaces. The adsorbed pro-angiogenic growth factors were also decreased. Furthermore, the CaP surfaces only stimulated angiogenesis when kept in the hemoderivative medium.
REGENERATIVE BIOMATERIALS
(2022)
Article
Pharmacology & Pharmacy
Toaa A. Abdelrahman, Amira Motawea, Marwa S. El-Dahhan, Galal M. Abdelghani
Summary: This study successfully formulated freeze-dried HPL onto CS/DHO thermo-sensitive scaffolds, achieving controlled temperature-responsive release of growth factors with good stability and cytocompatibility.
Article
Cell Biology
Aikaterini Gatsiou, Kateryna Sopova, Alexandros Tselepis, Konstantinos Stellos
Summary: Platelets are immune cells known for their haemostatic properties and play a key role in vascular regeneration and angiogenesis by releasing growth factors. The IL-17A/IL-17RA axis selectively induces the release of pro-angiogenic factors from platelets, affecting endothelial cells and driving them towards a pro-angiogenic state.
Article
Biotechnology & Applied Microbiology
Siddharth Shanbhag, Ahmad Rashad, Ellen Helgeland Nymark, Salwa Suliman, Catharina de Lange Davies, Andreas Stavropoulos, Anne Isine Bolstad, Kamal Mustafa
Summary: Cell coculture strategies using HUVEC and GPC spheroids in HPLG showed enhanced in vitro sprouting, suggesting a promising approach for promoting angiogenesis in tissue engineering constructs.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Sabrina Summer, Eva Rossmanith, Markus Pasztorek, Constantin Fiedler, Marion Groeger, Sabine Rauscher, Viktoria Weber, Michael B. Fischer
Summary: Mesenchymal stem cells play a crucial role in supporting endothelial cells during tissue regeneration and new vessel formation. By co-culturing these cells in a specific microenvironment, complex vascular networks can be formed. Under dynamic flow conditions, mesenchymal stem cells can bind to damaged endothelial cells, potentially aiding in the healing process.
Article
Biochemistry & Molecular Biology
Sarah Berndt, Gilles Carpentier, Antoine Turzi, Frederic Borlat, Muriel Cuendet, Ali Modarressi
Summary: Platelet-derived preparations such as PRP and PRP-HA show effective modulation in promoting therapeutic angiogenesis in vitro, suggesting their potential as biological therapeutic tools for tissue repair and regenerative processes.
Article
Biochemistry & Molecular Biology
Ching-Cheng Tsai, Tai-Horng Young, Guang-Shih Chen, Nai-Chen Cheng
Summary: A chitosan/gelatin hydrogel crosslinked by glyoxal was fabricated for sustained release of HPL, showing significant promotion of cell proliferation and migration. The growth factors released from the hydrogel exhibited sustained release characteristics, and HPL encapsulation also stimulated angiogenesis in ovo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Isabela Gobbo Ferreira, Manuela Berto Pucca, Isadora Sousa de Oliveira, Felipe Augusto Cerni, Beatriz de Cassia da Silva Jacob, Eliane Candiani Arantes
Summary: VEGF is a crucial regulator of angiogenesis in both physiological and pathological conditions. It is categorized into seven families, with different types found in mammals, viruses, and snake venoms. This review focuses on the svVEGF family, exploring its distribution, molecular structure, functions, and potential applications within snake venoms.
CYTOKINE & GROWTH FACTOR REVIEWS
(2021)
Article
Biochemistry & Molecular Biology
Michaela Oeller, Heidi Jaksch-Bogensperger, Markus Templin, Renate Gehwolf, Eva Rohde, Katharina Schallmoser, Sandra Laner-Plamberger
Summary: This study investigated the effect of human platelet lysate (HPL) on cell cycle gene expression in human stromal cells. The results showed that HPL can enhance cell proliferation by upregulating the expression of STAT3 and MYC, which are downstream effectors of tyrosine kinase receptor (TKR) pathways. This finding provides valuable insights into the molecular mechanism of HPL's growth-enhancing effect.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Aman S. Chahal, Manuel Gomez-Florit, Rui M. A. Domingues, Manuela E. Gomes, Hanna Tiainen
Summary: The study confirmed that PL-loaded hydrogel constructs have the ability to attract stem cells for endogenous tissue engineering purposes, with hMSCs exhibiting directional migration towards the PL-PEG hydrogel with increased velocity and directness.
Review
Food Science & Technology
Diana I. Meira, Ana I. Barbosa, Joel Borges, Rui L. Reis, Vitor M. Correlo, Filipe Vaz
Summary: Global population growth has a significant impact on the global food industry, posing a threat to food safety and quality. Mycotoxins, particularly Ochratoxin-A (OTA), are produced by fungus that contaminates various food species and products. OTA not only endangers food production but also poses potential toxicological risks to humans, leading to carcinogenic and neurological diseases. Therefore, the development of a selective, sensitive, and reliable OTA biodetection approach is crucial for ensuring food safety.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Review
Nanoscience & Nanotechnology
Prithwish Kola, Prasanth Kumar Bhusetty Nagesh, Pritam Kumar Roy, K. Deepak, Rui Luis Reis, Subhas C. Kundu, Mahitosh Mandal
Summary: The increasing number of breast cancer cases globally and the rising death rate indicate the inadequacy of traditional and current treatments. Nanoparticle-based therapies such as photothermal therapy, photodynamic therapy, chemodynamic therapy, and sonodynamic therapy show promise in combating breast cancer through accurate drug delivery and elimination of cancer stem cells. These innovative therapies have fewer side effects compared to standard chemotherapy and address the stability issues associated with cancer immunotherapy. This review discusses various nanotheranostic systems and smart nanoparticles, their mechanisms of action, and their relevance in the current era.
WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
(2023)
Review
Engineering, Biomedical
Ana M. Carvalho, Rui L. Reis, Iva Pashkuleva
Summary: The tumor microenvironment (TME) is a dynamic and complex environment shaped by heterogenous cancer and cancer-associated cells present at the tumor site. Hyaluronan (HA), a major component of TME, plays a role in promoting tumor growth and carcinogenesis. The interaction of different hyaladherins with HA triggers downstream signaling pathways, determining cell fate and contributing to TME progression towards a carcinogenic state.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Manuel Somoza, Ramon Rial, Zhen Liu, Iago F. Llovo, Rui L. Reis, Jesus Mosqueira, Juan M. Ruso
Summary: This study successfully created doped Hydroxyapatite nanoparticles with magnetic properties using microfluidics and Gadolinium as a contrast agent for medical applications. Computational Fluid Dynamics (CFD) was used to ensure the system worked in the laminar regime and nanoparticles diffused uniformly. The biomaterials were characterized using XRD, FE-SEM, EDX, confocal Raman microscopy, and FT-IR, confirming the successful incorporation of Gadolinium. Magnetic characterization confirmed the paramagnetic behavior of the nanoparticles, showing the potential for advanced nanomaterials in theragnostic applications.
Article
Chemistry, Medicinal
Lingzhi Wang, Catarina Oliveira, Qiu Li, Andreia S. Ferreira, Claudia Nunes, Manuel A. Coimbra, Rui L. Reis, Albino Martins, Chunming Wang, Tiago H. Silva, Yanxian Feng
Summary: This study characterized a pharmaceutical-grade fucoidan extracted from Fucus vesiculosus and investigated its anti-inflammatory potential. The extract contained mainly fucose, followed by uronic acids, galactose, and xylose, with a molecular weight of 70 kDa and a sulfate content of around 10%. In vitro and in vivo experiments demonstrated the ability of the fucoidan extract to modulate cytokine expression and reverse inflammation induced by lipopolysaccharide.
Article
Biochemistry & Molecular Biology
Lara L. Reys, Simone S. Silva, Diana Soares da Costa, Luisa C. Rodrigues, Rui L. Reis, Tiago H. Silva
Summary: The study aimed to create agarose/fucoidan hydrogels as a potential biomaterial for diabetes therapeutics. The hydrogels were produced by combining fucoidan and agarose, marine polysaccharides derived from seaweeds, and a thermal gelation process. Rheological tests showed a non-Newtonian and viscoelastic behavior, and the mechanical behavior indicated that increasing agarose concentrations resulted in hydrogels with higher Young's modulus. Encapsulating pancreatic cells in the hydrogels showed the ability to sustain cell viability and promote self-organization of pancreatic beta cells into pseudo-islets.
Article
Biochemistry & Molecular Biology
Cristina V. Rodrigues, Rita O. Sousa, Ana C. Carvalho, Ana L. Alves, Catarina F. Marques, Mariana T. Cerqueira, Rui L. Reis, Tiago H. Silva
Summary: The potential of Atlantic codfish skin collagen for skincare was evaluated in this study. The extracted collagen showed similar characteristics to bovine skin collagen and was not cytotoxic to keratinocytes. Collagen membranes developed from the extract exhibited smooth surfaces, good water absorption capacity, and improved metabolic activity and proliferation of keratinocytes. Therefore, these membranes have potential applications in the biomedical and cosmeceutical fields.
Article
Polymer Science
Duarte Nuno Carvalho, Flavia C. M. Lobo, Luisa C. Rodrigues, Emanuel M. M. Fernandes, David S. S. Williams, Andrew Mearns-Spragg, Carmen G. G. Sotelo, Ricardo I. I. Perez-Martin, Rui L. L. Reis, Michael Gelinsky, Tiago H. H. Silva
Summary: The self-repair capacity of human tissue is limited, leading to the development of tissue engineering for tissue regeneration. This study proposes the development and characterization of innovative polymeric membranes formed by blending marine origin polymers as biomaterials for tissue regeneration. The polymeric membranes demonstrated promising chemical and physical properties, suitable for tissue engineering approaches, particularly for the regeneration of damaged articular cartilage.
Article
Biotechnology & Applied Microbiology
Silvia Vieira, Paulina Strymecka, Luiza Stanaszek, Joana Silva-Correia, Katarzyna Drela, Michal Fiedorowicz, Izabela Malysz-Cymborska, Miroslaw Janowski, Rui Luis Reis, Barbara Lukomska, Piotr Walczak, Joaquim Miguel Oliveira
Summary: This study aims to develop a stable injectable Mn-based methacrylated gellan gum (Mn/GG-MA) hydrogel for real-time monitored cell delivery into the central nervous system. By supplementing GG-MA solutions with paramagnetic Mn2+ ions, the hydrogel can be visualized under Magnetic Resonance Imaging (MRI). Cell-laden hydrogels prepared using the Mn/GG-MA formulations remained viable after 7 days of culture. In vivo tests on immunocompromised mice showed that the injection of Mn/GG-MA solutions resulted in a continuous and traceable hydrogel visible on MRI scans. Overall, the developed formulations are suitable for non-invasive cell delivery techniques and image-guided neurointerventions.
BIOENGINEERING-BASEL
(2023)
Article
Chemistry, Medicinal
Simone S. Silva, Luisa C. Rodrigues, Emanuel M. Fernandes, Diana Soares da Costa, Denise G. Villalva, Watson Loh, Rui L. Reis
Summary: This study reports on an emulsion system combining chitosan (CHT) and virgin coconut oil (VCO) to develop structures with potential for biomedical applications. The scaffolds produced through freezing and freeze-drying possessed a porous structure, high swelling ability, and suitable stiffness. Moreover, the sustained release of the encapsulated drug from the cubosomes into the CHT/VCO-based system was achieved. Overall, this approach provides a new avenue for designing porous biomaterials for drug delivery.
Review
Biotechnology & Applied Microbiology
Lara Pierantoni, Rui L. Reis, Joana Silva-Correia, Joaquim M. Oliveira, Susan Heavey
Summary: The fields of tissue bioengineering, -omics, and spatial biology offer the opportunity for a paradigm shift in breast cancer research. However, collaboration between these fields has not reached its full potential. This review discusses the latest 3D breast cancer models, their biomaterials and technological platforms, as well as their biological evaluation, highlighting the potential for better microphysiological systems, improved drug development success rates, and personalized medicine approaches.
TRENDS IN BIOTECHNOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Rui R. Costa, David Caballero, Diana Soares da Costa, Romen Rodriguez-Trujillo, Subhas C. Kundu, Rui L. Reis, Iva Pashkuleva
Summary: Anisotropy plays a crucial role in the organization and healing of neural tissues, and holds great potential for developing regeneration strategies. The study demonstrates the fabrication of microfibers from charged ECM components using interfacial polyelectrolyte complexation, which can mimic the mechanical properties of neural tissues. These fibers are biocompatible and promote the formation of neural processes, enabling the development of neural cells.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Biology
Temuulen Selenge, Sara F. Vieira, Odontuya Gendaram, Rui L. Reis, Soninkhishig Tsolmon, Enkhtuul Tsendeekhuu, Helena Ferreira, Nuno M. Neves
Summary: In this study, the ethanol and dichloromethane extracts of Stellera chamaejasme were evaluated for their antioxidant and anti-inflammatory activities. The ethanol extract of the aerial parts contained flavonoid compounds, while the root extracts and dichloromethane extract did not. The extracts exhibited strong antioxidant activity and efficiently inhibited the production of pro-inflammatory cytokines. Particularly, the dichloromethane extract of the root showed the strongest activity and contained fatty acids and triterpenoids.
Article
Materials Science, Biomaterials
Ana M. Martins, Alexandra Brito, Maria Grazia Barbato, Alessia Felici, Rui L. Reis, Ricardo A. Pires, Iva Pashkuleva, Paolo Decuzzi
Summary: The 3D organization of cells plays a significant role in their behavior and response to treatment. In cancer research, spheroids, organoids, and microfluidic chips are used to mimic the complex microenvironment of tumors. This study utilized microfluidic devices to replicate the spatial organization of brain tumors and evaluate the efficacy of anti-cancer agents. The results showed that brain tumor cells were more sensitive to treatment in the microfluidic device compared to traditional cell cultures. The proposed microfluidic chips can effectively reproduce the 3D structure of tumors and assess the effectiveness of therapeutic compounds.
BIOMATERIALS ADVANCES
(2023)
Article
Green & Sustainable Science & Technology
Fatemeh Soltanzadeh, Ali Edalat-Behbahani, Kasra Hosseinmostofi, Ibrahim Fatih Cengiz, Joaquim Miguel Oliveira, Rui L. Reis
Summary: This study investigated the bond durability characteristics of basalt-fiber-reinforced polymer (BFRP) rebars in fiber-reinforced self-compacting concrete (FRSCC) structures. The influence of environmental conditions, reinforcement type, and loading type on the bond strength of the specimens was explored. The results were used to estimate the bond strength retention between BFRP and FRSCC after 50 years of exposure to seawater.