Article
Chemistry, Multidisciplinary
Brian J. J. Timmer, Arjaan Kooijman, Xander Schaapkens, Tiddo J. Mooibroek
Summary: Galectins are a subclass of carbohydrate binding proteins that play a role in various biological processes. A synthetic galectin mimic with high selectivity for a specific galactoside has been designed and synthesized for the first time, showing a selectivity over 65-fold compared to a glucoside.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Editorial Material
Biochemistry & Molecular Biology
Elizabeth Ficko-Blean
Summary: The study investigates the ligand specificity of the human mannose receptor, particularly focusing on carbohydrate-recognition domain 4, and elucidates the molecular mechanisms driving binding specificity. The data contribute to understanding Ca2+-mediated binding promiscuity in the receptor and highlight unexpected interactions for further study.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Ghulam Md Ashraf, Mohd Rehan, Alhuseen O. Alsayed, Pallavi Somvanshi, Shafiul Haque
Summary: This study examines the possibility of cardiovascular medications inhibiting Galectin-3-related biological activities using bioinformatics techniques. Through clustering, molecular docking, and molecular dynamics simulation, potential chemical compounds were screened and repurposed. The results show that certain compounds have the ability to inhibit Galectin-3. Further experimental validation and animal model-based studies are needed.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Dong Kyu Kim, Hyun Joo Lee, Jiwon Kong, Ha Yeon Cho, Sunghoon Kim, Beom Sik Kang
Summary: In this study, crystal structures of the human MRS catalytic main body and the complex of the GST domain and AIMP3 were determined. The structures reveal human-specific details of MRS and provide a dynamic model for understanding the domain orientation of MRS. This study suggests a dynamic switching between two possible MRS conformations based on the position of the GST domain relative to the catalytic main body.
NUCLEIC ACIDS RESEARCH
(2021)
Review
Pharmacology & Pharmacy
Selena Bouffette, Iuliana Botez, Frederic De Ceuninck
Summary: This article reviews the key role of Galectin-3 (Gal-3) in cardiac, hepatic, renal, and pulmonary fibrosis and inflammation, respiratory infections caused by COVID-19, and neuroinflammatory disorders. The recent strategic breakthroughs have led to the identification of new-generation Gal-3 inhibitors with improved potency, selectivity, and bioavailability, which have shown promise as valuable tools in proof-of-concept studies in preclinical models of these diseases, particularly those in clinical stages. Critical views and suggestions to expand the therapeutic opportunities provided by this complex target are also discussed.
TRENDS IN PHARMACOLOGICAL SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Kotaro Nakanishi
Summary: This review explores the completed structural catalog of the four AGO proteins, describing their common features and differences in target specificity, and emphasizing the unique roles of each AGO.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Multidisciplinary Sciences
Valerie M. Tesmer, Kirsten A. Brenner, Jayakrishnan Nandakumar
Summary: The study reveals that human POT1 binds the double-stranded-single-stranded junction of telomeres by recognizing the phosphorylated 5' end of the chromosome. This binding is achieved through a POT-hole surface, and mutation of this surface compromises the protection of the junction and the suppression of DNA damage response in human cells. Only POT1a, not POT1b, contains the POT-hole and is sufficient for end protection.
Article
Biochemistry & Molecular Biology
Maria H. C. Santos, Valdenice F. Santos, Priscilla R. Freitas, Renato Rodrigues Roma, Ana L. E. Santos, Daiany Alves Ribeiro, Henrique D. M. Coutinho, Bruno A. M. Rocha, Manoel M. E. Oliveira, Claudener S. Teixeira
Summary: This study evaluated the interaction between DVL and neomycin via CRD and the ability of DVL to modulate the antibiotic effect of neomycin against multidrug-resistant strains (MDR). The results showed that neomycin interacts with DVL via the CRD and that DVL enhances the antibiotic activity of neomycin against S. aureus and P. aeruginosa.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Yebing Liang, Yuxiang Wang, Xingyu Zhu, Jun Cai, Anqi Shi, Jing Huang, Qiuju Zhu, Yunlong Si
Summary: Glycerol is an intermediate in lipid metabolism and has been found to act as a chemical chaperone for protein conformation. This study investigates the role of glycerol in Gal-7 and demonstrates that glycerol increases the thermal stability of Gal-7 and inhibits erythrocyte agglutination. Crystal structures reveal glycerol binding sites in Gal-7, suggesting its potential as a ligand for Gal-7. Overexpression of Gal-7 also affects glycerol metabolism and triglyceride levels in cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Rebecca Brinkschulte, David M. Fusshoeller, Florian Hoss, Juan F. Rodriguez-Alcazar, Mario A. Lauterbach, Carl-Christian Kolbe, Melanie Rauen, Semra Ince, Christian Herrmann, Eicke Latz, Matthias Geyer
Summary: This study investigates the intrinsic ATP hydrolysis activity of the inflammasomal protein NLRP3 and reveals the activity difference in different conformational states. Site-directed mutagenesis of canonical residues in the nucleotide binding site is analyzed and it is found that the NLRP3 hydrolysis activity correlates with inflammasome activity.
COMMUNICATIONS BIOLOGY
(2022)
Article
Fisheries
Chaehyeon Lim, Seongdo Lee, Hyukjae Kwon, W. M. Gayashani Sandamalika, Jehee Lee
Summary: This study aimed to investigate the binding ability and potential role of LhGal B-like in pattern recognition receptors (PRRs) and innate immunity in redlip mullet. LhGal B-like was found to be widely expressed in various tissues, with the highest expression in the brain, gills, and muscle. It was also observed that LhGal B-like had immune-modulated expression and could bind to carbohydrates, PAMP molecules, and various bacterial strains.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biophysics
Supriyo Bhattacharya, Mingfeng Zhang, Weidong Hu, Tong Qi, Nora Heisterkamp
Summary: Intrinsically disordered regions (IDRs) are common and important functional domains in proteins, but hard to target for drug development. This study successfully predicted the binding interface of Galectin-3's disordered N-terminal domain (NTD) with the structured C-terminal domain (CTD) and designed inhibitory peptides targeting this interface using a combination of nuclear magnetic resonance data and accelerated molecular dynamics simulations.
BIOPHYSICAL JOURNAL
(2022)
Review
Chemistry, Medicinal
Nerella Sridhar Goud, Ahana Bhattacharya
Summary: Gal-1, a carbohydrate-binding protein found in humans, plays a crucial role in tumor progression in various cancers, affecting processes such as tumor transformation, cell cycle regulation, apoptosis, cell adhesion, migration, and inflammation. Its interaction with carbohydrates on cell-surface, cytosol, and nucleus regulates cellular functions, making it a promising molecular target for new therapeutic tools.
MINI-REVIEWS IN MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Dong Man Jang, Eun Kyung Oh, Hyunggu Hahn, Hyoun Sook Kim, Byung Woo Han
Summary: This study reports the crystal structure of human full-length Bfk, revealing its canonical Bcl-2 fold but lacking the hydrophobic cleft. Molecular dynamics simulation and biophysical interaction analysis demonstrate that Bfk elicits its pro-apoptotic activity through a Bid-like apoptotic mechanism.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Multidisciplinary Sciences
Sarah Willkomm, Leonhard Jakob, Kevin Kramm, Veronika Graus, Julia Neumeier, Gunter Meister, Dina Grohmann
Summary: Human Argonaute 2 (hAgo2) plays a key role in the RNA interference pathway. Through single-molecule FRET measurements, this study provides detailed insights into the conformational states and dynamics of hAgo2 and its RNA complexes, revealing how their flexibility determines the function and fate of the ribonucleoprotein particle.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Jiyong Su, Tao Zhang, Peiqi Wang, Fengjian Liu, Guihua Tai, Yifa Zhou
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2015)
Article
Chemistry, Physical
Yunlong Si, Ye Yuan, Yue Wang, Jin Gao, Yanbo Hu, Shiqiong Feng, Jiyong Su
Article
Biochemistry & Molecular Biology
Jiyong Su, Karl Forchhammer
Article
Biochemistry & Molecular Biology
Jiyong Su, Christine Schlicker, Karl Forchhammer
JOURNAL OF BIOLOGICAL CHEMISTRY
(2011)
Article
Biochemistry & Molecular Biology
Jiyong Su, Linlin Cui, Yunlong Si, Chenyang Song, Yuying Li, Tong Yang, Hao Wang, Kevin H. Mayo, Guihua Tai, Yifa Zhou
BIOSCIENCE REPORTS
(2018)
Review
Biochemistry & Molecular Biology
Jiyong Su
Article
Biochemistry & Molecular Biology
Yunlong Si, Xing Wang, Guosong Yang, Tong Yang, Yuying Li, Gabriela Jaramillo Ayala, Xumin Li, Hao Wang, Jiyong Su
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Biochemistry & Molecular Biology
Yunlong Si, Yuying Li, Tong Yang, Xumin Li, Gabriela Jaramillo Ayala, Kevin H. Mayo, Guihua Tai, Jiyong Su, Yifa Zhou
Summary: The crystal structure of Gal-14 differs significantly from other galectins, particularly in its unique interaction with carbohydrates and lactose. In addition, Gal-14 exhibits distinctive characteristics in terms of agglutination activity and cellular localization compared to other galectins.
Article
Biochemistry & Molecular Biology
Yunlong Si, Yue Wang, Jin Gao, Chenyang Song, Shiqiong Feng, Yifa Zhou, Guihua Tai, Jiyong Su
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2016)