Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins

Structural Mechanism for Modulation of Synaptic Neuroligin-Neurexin Signaling by MDGA Proteins

(2017) Jonathan Elegheert et al.   NEURON

Generation of a new Gateway-compatible inducible lentiviral vector platform allowing easy derivation of co-transduced cells

(2016) Philippe De Groote et al.   BIOTECHNIQUES

Structural basis of Smoothened regulation by its extracellular domains

(2016) Eamon F. X. Byrne et al.   NATURE

Initiation of T cell signaling by CD45 segregation at 'close contacts'

(2016) Veronica T Chang et al.   NATURE IMMUNOLOGY

Structural basis for integration of GluD receptors within synaptic organizer complexes

(2016) Jonathan Elegheert et al.   SCIENCE

Lentiviral vectors, two decades later

(2016) L. Naldini et al.   SCIENCE

Manipulation of Subunit Stoichiometry in Heteromeric Membrane Proteins

(2016) Claudio L. Morales-Perez et al.   STRUCTURE

IVA cloning: A single-tube universal cloning system exploiting bacterial In Vivo Assembly

(2016) Javier García-Nafría et al.   Scientific Reports

Repulsive guidance molecule is a structural bridge between neogenin and bone morphogenetic protein

(2015) Eleanor G Healey et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Flow cytometry strikes gold

(2015) J. Paul Robinson et al.   SCIENCE

Repulsive guidance molecule is a structural bridge between neogenin and bone morphogenetic protein

(2015) Eleanor G Healey et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Crystal structure of a human GABAA receptor

(2014) Paul S. Miller et al.   NATURE

Screening and large-scale expression of membrane proteins in mammalian cells for structural studies

(2014) April Goehring et al.   Nature Protocols

A streamlined implementation of the glutamine synthetase-based protein expression system

(2013) Rachel Knox et al.   BMC BIOTECHNOLOGY

Expression of recombinant glycoproteins in mammalian cells: towards an integrative approach to structural biology

(2013) A Radu Aricescu et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Simple piggyBac transposon-based mammalian cell expression system for inducible protein production

(2013) Z. Li et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

LDL receptor and its family members serve as the cellular receptors for vesicular stomatitis virus

(2013) D. Finkelshtein et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Development of the Twin-Strep-tag® and its application for purification of recombinant proteins from cell culture supernatants

(2013) Thomas G.M. Schmidt et al.   PROTEIN EXPRESSION AND PURIFICATION

Structure of the Repulsive Guidance Molecule (RGM)-Neogenin Signaling Hub

(2013) C. H. Bell et al.   SCIENCE

Flow cytometry of fluorescent proteins

(2012) William G. Telford et al.   METHODS

Improving FRET dynamic range with bright green and red fluorescent proteins

(2012) Amy J Lam et al.   NATURE METHODS

Overexpressing human membrane proteins in stably transfected and clonal human embryonic kidney 293S cells

(2012) Sarika Chaudhary et al.   Nature Protocols

A Fluorescence-Detection Size-Exclusion Chromatography-Based Thermostability Assay for Membrane Protein Precrystallization Screening

(2012) Motoyuki Hattori et al.   STRUCTURE

Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%

(2012) Joachim Goedhart et al.   Nature Communications

The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability

(2011) Mattia Matasci et al.   BIOTECHNOLOGY AND BIOENGINEERING

Daedalus: a robust, turnkey platform for rapid production of decigram quantities of active recombinant proteins in human cell lines using novel lentiviral vectors

(2011) Ashok D. Bandaranayake et al.   NUCLEIC ACIDS RESEARCH

High Cleavage Efficiency of a 2A Peptide Derived from Porcine Teschovirus-1 in Human Cell Lines, Zebrafish and Mice

(2011) Jin Hee Kim et al.   PLoS One

Development of an All-in-One Lentiviral Vector System Based on the Original TetR for the Easy Generation of Tet-ON Cell Lines

(2011) Karim Benabdellah et al.   PLoS One

Targeted methylation of CMV and E1A viral promoters

(2010) Chia-Chen Hsu et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Generation of stable, high-producing cho cell lines by lentiviral vector-mediated gene transfer in serum-free suspension culture

(2010) Agata Oberbek et al.   BIOTECHNOLOGY AND BIOENGINEERING

Systematic Comparison of Constitutive Promoters and the Doxycycline-Inducible Promoter

(2010) Jane Yuxia Qin et al.   PLoS One

Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectors

(2009) Robert H Kutner et al.   Nature Protocols

A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells

(2009) Eric Campeau et al.   PLoS One

Valproic acid: A viable alternative to sodium butyrate for enhancing protein expression in mammalian cell cultures

(2008) Gaurav Backliwal et al.   BIOTECHNOLOGY AND BIOENGINEERING

BacMam system for high-level expression of recombinant soluble and membrane glycoproteins for structural studies

(2008) Abhiram Dukkipati et al.   PROTEIN EXPRESSION AND PURIFICATION