Article
Biophysics
Wenyuan Kang, Lin Liu, Peihang Yu, Tianyi Zhang, Chunyang Lei, Zhou Nie
Summary: The study demonstrates the use of a switchable Cas12a for direct analysis of histone deacetylase (HDAC) activity. The inactivation of Cas12a by acetylation can be reversed by HDAC-mediated deacetylation. By leveraging this switchable Cas12a, a sensitive one-pot assay for detecting HDAC activity was developed, with sub-nanomolar sensitivity. This assay can be applied in biochemical studies and clinical diagnostics.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Electrochemistry
Amina Rhouati, Jean-Louis Marty, Alina Vasilescu
Summary: The review discusses the powerful tool of combining aptamers' specific recognition abilities and enzymes' catalytic power for highly selective and sensitive detection in bioanalysis. The use of enzymes and DNAzymes as amplification methods, along with the integration of nucleic acid catalysts into aptazymes, are highlighted as preferred approaches in electrochemical aptasensors.
ELECTROCHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Shu-Ying Ye, Chang-Gang Pan, Yu-Hua Dai, Guo-Xi Liang
Summary: An ultrasensitive biosensor for the detection of telomerase activity was constructed utilizing gold@Carbon dots nanohybrids (Au@CDs) as a high efficiency electrochemiluminescence (ECL) emitter and nicking enzyme assisted signal amplification (NESA) technology. By employing a dual signal amplification strategy, the biosensor showed excellent sensitivity and detection limit for telomerase activity.
MICROCHEMICAL JOURNAL
(2021)
Article
Nanoscience & Nanotechnology
Jiajia Huang, Lei Jiao, Weiqing Xu, Qie Fang, Hengjia Wang, Xiaoli Cai, Hongye Yan, Wenling Gu, Chengzhou Zhu
Summary: Enzyme immobilization is crucial for addressing the fragility of enzymes, but it may impact their bioactivity and catalytic efficiency. PdCu hydrogel nanozymes were used to immobilize horseradish peroxidase, resulting in improved stability and catalytic activity for biosensing of carcinoembryonic antigen. This approach showed promising potential for clinical diagnosis with significantly enhanced detection sensitivity.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Analytical
Tiantian Wang, Xiangguang Li, Lili Chen, Youhuan Zhang, Yujun Zheng, Linjin Yu, Zhiyu Ye, Huaqian Wang, Xiping Cui, Suqing Zhao
Summary: A new iELISA method based on nano-flowers was developed for sensitive detection of Helicobacter pylori. The method showed linearity and improved stability compared to traditional methods, offering a sensitive, low-cost and convenient option for non-invasive HP detection.
Article
Chemistry, Analytical
Kexin Ding, Yantian Shi, Yaojia Liu, Xinyue Song, Haiyan Wang, Jing Liu, Hong Zhou
Summary: We developed an enzyme-assisted multiple signal amplification reaction for target nucleic acid detection. This strategy involves utilizing the target nucleic acid as triggers, along with specific primers, DNA polymerase, and DNA nicking endonuclease, to promote repetitive cycle DNA replication and exponentially increase the nucleic acid sequence. The amplification products form a Mg2+-DNAzyme unit with the specific primer, enabling real-time fluorescence detection of HIV-1 DNA through cyclic cleavage of hairpin probes. Under optimal conditions, this method has a detection limit of 10-17 M. It offers a simple design, isothermal conditions, and easy fluorescence measurement, making it highly sensitive and specific for quantitative detection of DNA or RNA in theoretical and clinical studies.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Xiaofan Zhang, Shunjun Xie, Xue Chen, Li Wang, Fang Li, Shufeng Liu
Summary: A new catalytic DNA circuit reaction strategy has been proposed for sensitive nucleic acid detection, utilizing DNA switch-mediated reactions to achieve signal amplification and ratiometric signal response mode, enabling detection limits as low as 0.1 pM and selective discrimination towards different mismatched targets.
ANALYTICAL METHODS
(2022)
Review
Biotechnology & Applied Microbiology
Yanna Shao, Huan Zhou, Qingping Wu, Yonghua Xiong, Juan Wang, Yu Ding
Summary: This study systematically discusses the importance of enzyme-based signal transduction patterns in immunoassay, as well as different strategies for enhancing the output signals of enzymes to significantly improve the sensitivity of enzyme-based immunosensors. The article categorizes these strategies into three categories and discusses the current focus and future direction of enzyme-based immunoassays.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Chemistry, Analytical
Pinghua Ling, Linyu Wang, Shan Cheng, Xianping Gao, Xinyu Sun, Feng Gao
Summary: A cost-effective, simple, and sensitive electrochemical sensing platform was developed based on aptamer target recognition and signal amplification strategy for protein detection. The method showed good selectivity and sensitivity for a variety of protein targets and had the potential for application in bioassays.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Yulian Tang, Xue Lv, Wenxin Gou, Xuemei Zhou, Junkai Hao, Jing Feng, Yuji Qi, Lei Hu, Zhengquan Yan
Summary: A bioactive folic acid-enhanced silver nanozyme was developed for efficient and convenient monitoring of dopamine. The nanozyme exhibited superior peroxidase-like activity and selective recognition for dopamine, enabling visual detection through color change. The method showed good recovery and stability when applied to real samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Chen-Chen Bai, Meng-Ying Chen, Tai-Cheng Zhou, Ruo-Lin Jiang, Lin-Yi Dong, Hua-Wei Wei, Xiang-Jin Kong, Xian-Hua Wang
Summary: In this study, a novel HRBGO nanocomposite was fabricated to substitute the complex and time-consuming process of enzyme-labeled secondary antibody preparation. The HRBGO selectively labeled the carbohydrate chains of the primary antibody's Fc fragment and achieved signal output and amplification through the release of rhodamine B. The proposed method demonstrated ultrahigh sensitivity and specificity in the detection of trastuzumab and was successfully applied in the analysis of real serum samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Analytical
Yi-Zhuo Fu, Xiang-Mei Liu, Shu-Hui Ma, Jun-Tao Cao, Yan-Ming Liu
Summary: This study developed an ECL immunosensor using a liposome-assisted enzyme catalysis signal amplification strategy for detecting PSA, with a wider linear range and lower detection limit. The sensor showed good recoveries and low RSD when analyzing PSA in human serum samples. The work opens up new possibilities for the application of liposomes in ECL bioanalysis.
Article
Chemistry, Analytical
Akbar Khanmohammadi, Abbas Afkhami, Ali Hajian, Hosein Khoshsafar, Hasan Bagheri
Summary: A sandwich-type electrochemical immunoassay was developed for the determination of PSA biomarker, utilizing a trimetallic composite for the capture layer and a bimetallic composite as a label. The immunoassay showed a linear relationship between PSA concentration and DPV responses, with a low limit of detection. Cross-reactivity studies demonstrated the immunoassay's specificity and reliability in the presence of interfering substances.
ANALYTICAL METHODS
(2021)
Article
Chemistry, Analytical
Hao Jiang, Yuan Li, Xuefei Lv, Yulin Deng, Xiaoqiong Li
Summary: Nucleic acid amplification techniques, such as polymerase chain reaction (PCR) and isothermal amplification, are important for nucleic acid detection. However, PCR is limited by temperature control and isothermal amplification has its own limitations. Efforts to integrate enzymes and amplification techniques have the potential to overcome these limitations. This review provides a comprehensive summary of cascade amplification, including design fundamentals, signal generation, evolution, applications, challenges, and trends.
Article
Chemistry, Multidisciplinary
Jintao Yi, Xianqin Han, Fengying Gao, Le Cai, Ying Chen, Xiulong Deng, Xun Li, Jun Xue, Hui Zhou
Summary: Researchers have developed a novel Ba-hemin@GOX biosensor for glucose detection using a simple biomineralization method, and have verified its operability and accuracy in real samples. The sensor exhibits a low detection limit, good selectivity, storage stability, and can be reused multiple times.
Article
Electrochemistry
Jing Zhao, Yingying Tang, Ya Cao, Tianshu Chen, Xiaoxia Chen, Xiaoxia Mao, Yongmei Yin, Guifang Chen
ELECTROCHIMICA ACTA
(2018)
Article
Chemistry, Analytical
Xiaoxia Mao, Yifei Li, Peng Han, Xiaohong Wang, Shiqi Yang, Fan Zhang, Xiuqing Gong, Ya Cao
SENSORS AND ACTUATORS B-CHEMICAL
(2018)
Article
Chemistry, Multidisciplinary
Chao Li, Xiaolu Hu, Jianyang Lu, Xiaoxia Mao, Yang Xiang, Yongqian Shu, Genxi Li
Article
Chemistry, Multidisciplinary
Xiaoxia Mao, Guifang Chen, Zihan Wang, Yuanguang Zhang, Xiaoli Zhu, Genxi Li
Article
Medicine, Research & Experimental
Xiaoxia Chen, Jing Zhao, Tianshu Chen, Tao Gao, Xiaoli Zhu, Genxi Li
Article
Chemistry, Analytical
Xiaoxia Mao, Shaobing Pan, Duoqi Zhou, Xiaowu He, Yuanguang Zhang
SENSORS AND ACTUATORS B-CHEMICAL
(2019)
Article
Nanoscience & Nanotechnology
Xiaoxia Mao, Dongsheng Mao, Tianshu Chen, Mohammed Jalalah, Mohammed S. Al-Assiri, Farid A. Harraz, Xiaoli Zhu, Genxi Li
ACS APPLIED MATERIALS & INTERFACES
(2020)
Article
Chemistry, Analytical
Xiaoxia Mao, Shaowei Liu, Benyue Su, Dejin Wang, Zhan Huang, Jian Li, Yuanguang Zhang
Article
Chemistry, Multidisciplinary
Shaowei Liu, Yumeng Yang, Mengqin Shi, Hai Shi, Dongsheng Mao, Xiaoxia Mao, Yuanguang Zhang
Summary: In this study, a portable smartphone-based DNAzyme hydrogel platform was developed for the detection of cfDNA. The platform showed high sensitivity and visibility, making it a promising tool for testing other nucleic acid biomarkers in the future.
Article
Chemistry, Analytical
Yuxin Lin, Beibei Nie, Xinyu Qu, Minghui Wang, Jie Yang, Genxi Li
Summary: Covalent organic frameworks (COFs) have attracted much attention for their unique physical-chemical characteristics and potential applications in biosensing. In this study, spherical COFs with regular shape and good dispersion were synthesized, and a novel nanoprobe was developed by modifying Histostar on the COFs surface. The nanoprobe was then applied to fabricate an electrochemical biosensor for exosomes detection, achieving a low detection limit and wide linear detection range. This work offers a promising platform for ultrasensitive detection of exosomes.
Article
Biochemical Research Methods
Xiaoxia Mao, Dongsheng Mao, Juanjuan Jiang, Benyue Su, Guifang Chen, Xiaoli Zhu
Summary: In this study, a semi-dry smart biosensing platform was developed for the sensitive detection of metal ions using a hydrogel and intelligent image recognition. The platform demonstrated favorable operability and performance, with detection limits of 0.34 mM for potassium ions and 5.6 nM for mercury ions. It was also capable of detecting ions in serum and lake water, showing promising potential for on-site testing.
Article
Nanoscience & Nanotechnology
Tao Gao, Chaoli Mu, Hai Shi, Liu Shi, Xiaoxia Mao, Genxi Li
ACS APPLIED MATERIALS & INTERFACES
(2018)
Article
Chemistry, Analytical
Cao Ya, Kang Ming-Yang, Chen Hong, Tang Ying-Ying, Feng Chang, Zhao Jing, Li Gen-Xi
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
(2017)
Article
Chemistry, Analytical
Juan Zhang, Xiaonan Wang, Tingjun Chen, Chang Feng, Genxi Li
ANALYTICAL CHEMISTRY
(2017)
Article
Chemistry, Multidisciplinary
Xiaolu Hu, Chao Li, Chang Feng, Xiaoxia Mao, Yang Xiang, Genxi Li
CHEMICAL COMMUNICATIONS
(2017)