期刊
JOURNAL OF CONSUMER PROTECTION AND FOOD SAFETY
卷 14, 期 2, 页码 147-155出版社
SPRINGER INTERNATIONAL PUBLISHING AG
DOI: 10.1007/s00003-018-1192-7
关键词
DNA extraction; Zeolite Y; Low DNA content food; Real-time PCR
资金
- TUBITAK TOVAG 1003 [115O065]
Recently, molecular biology techniques are widely used in food analysis; yet, the complexity of food matrices causes difficulty in purification and analysis of the analyte. The aim of this study was to introduce a new material, zeolite Y, that is able to isolate DNA even from low DNA content food samples; and, to demonstrate the integrity of isolated DNA via real-time polymerase chain reaction. Although the purity of the isolated DNA has to be improved with further work, Zeolite Y was shown to have a potential to be effectively used in DNA isolation for food analysis especially for low DNA content food samples.
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