Article
Immunology
Jun Yang, Xiao-li Yang, Yu-bin Su, Xuan-xian Peng, Hui Li
Summary: Vaccines are safe and effective in controlling bacterial diseases in the aquaculture industry and align with green farming. A novel approach of preparing live-attenuated V. alginolyticus vaccine using high magnesium concentration to attenuate virulence was developed. Metabolomic analysis revealed enhanced TCA cycle and increased fumarate as crucial pathways and biomarkers in vaccine-mediated and survival fish.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Fisheries
Yanni Zhao, Jiamin Ren, Heyang Jiang, Xuefeng Chen, Mudan Xu, Yue Li, Jieyu Zhao, Dan Chen, Kun Zhang, Hua Li, Huan Liu
Summary: Vibrio alginolyticus is a significant aquaculture pathogen, with Hfq deletion leading to increased de novo synthesis of fatty acids and nucleotides, altered lipid metabolism affecting cell division and growth, suppression of branched amino acid and aromatic amino acid synthesis, and enhanced TCA cycle to impact cell stress defenses and energy metabolism.
Article
Biotechnology & Applied Microbiology
Yanni Zhao, Ningning Zhou, Jiamin Ren, Wang Liu, Chuang Zhou, Xuefeng Chen, Jieyu Zhao, Juanjuan Cao, Jinfang Yang, Jie Han, Huan Liu
Summary: This study comprehensively investigates the regulatory roles of the new cell density-dependent sRNA Qrr4 in V. alginolyticus, revealing its significant effects on the physiology, virulence, and metabolism of the bacteria. The disturbance of multiple metabolic pathways, including phospholipid, nucleotide, carbohydrate, and amino acid metabolic pathways, provides novel insights into the potential mechanism by which mutation of qrr4 interferes with cellular energy homeostasis and modulates the characteristics of V. alginolyticus.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Fisheries
Yameng He, Xin Li, Chenyu Shi, Yin Li, Qi Li, Shikai Liu
Summary: In this study, RNA-seq analysis of Pacific oyster gill tissue was performed to investigate the immune response to bacterial infection. The results showed that the immune strategy of the oyster changed significantly at short-term and long-term stages, with activation of immune-related genes with different functions. This work provides valuable insights into immune response, disease monitoring, and disease resistance breeding.
Article
Microbiology
Yue Yin, Yuanpan Yin, Hao Yang, Zhuanggui Chen, Jun Zheng, Bo Peng
Summary: Antibiotic-resistant Vibrio alginolyticus is a global challenge for human health and food safety. This study reveals that metabolic modulation could be a novel approach to enhance antibiotic efficacy. By comparing the metabolic profiles of bacteria treated with different concentrations of ofloxacin, it is found that bacteria adapt to antibiotic stress by altering metabolic flux, with disruption of the pyruvate cycle and elevation of fatty acid biosynthesis playing crucial roles.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Fisheries
Keng Chin Lim, Fatimah Md. Yusoff, Mohamed Shariff, Mohd Salleh Kamarudin
Summary: Supplementation with astaxanthin significantly improves hematological parameters, serum biochemical profile, and immunological defense mechanisms of the Asian seabass against Vibrio alginolyticus infection, leading to enhanced survival rate.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Chang Guo, Jing-zhou Ye, Min Song, Xuan-xian Peng, Hui Li
Summary: The study reveals that poly I:C can enhance innate immunity against bacterial infection by boosting the TCA cycle.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Shangyu Zhai, Ben Yang, Fuqiang Zhang, Qi Li, Shikai Liu
Summary: The Pacific oyster showed high phenotypic variation in resistance to Vibrio alginolyticus infection, with low to moderate heritability estimates for resistance. High correlation coefficients among family estimated breeding values suggest consistent predictive ability of different models for family EBVs. The low genetic and phenotypic correlation with growth traits indicate the feasibility of simultaneous genetic improvement in both growth and resistance traits.
Article
Immunology
Su-fang Kuang, Yue-tao Chen, Jia-jie Chen, Xuan-xian Peng, Zhuang-gui Chen, Hui Li
Summary: The study found that the cooperative effect of alanine and gentamicin can reduce the level of nitric oxide, which plays a key role in the process of alanine-potentiated gentamicin killing.
Article
Fisheries
He-Jia Chen, Pin -Hong Li, Yun Yang, Xiao-Hong Xin, Yan Ou, Jing -Guang Wei, You-Hua Huang, Xiao-Hong Huang, Qi-Wei Qin, Hong-Yan Sun
Summary: Heat shock protein 40 (Hsp40), a member of the Heat shock proteins (Hsps) family, plays a crucial role in regulating cell proliferation, survival, and apoptosis in marine-cultured fish. The expression of EcHsp40 in Epinephelus coioides is significantly up-regulated in response to bacterial or viral challenges, suggesting its involvement in pathogenic stimulation-induced apoptosis and viral replication.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Environmental Sciences
Enshuo Zhang, Luyao Dong, Xiaokai Bao, Xinyu Yang, Yuxin Li, Yanwei Feng, Jianmin Yang, Zan Li, Weijun Wang
Summary: In this study, a transcriptome analysis was conducted on Pacific oysters infected with Vibrio alginolyticus, revealing differentially expressed genes (DEGs) related to immunity. A protein-protein interaction network was constructed to investigate the immune defense mechanism of the oyster. Key genes involved in immune-related signaling pathways were screened and validated. This research provides insights for addressing the problem of high mortality in oyster farming during summer and offers references for the future development of disease-resistant oysters.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Microbiology
Stavros Droubogiannis, Lydia Pavlidi, Maria Ioanna Tsertou, Constantina Kokkari, Dimitrios Skliros, Emmanouil Flemetakis, Pantelis Katharios
Summary: A novel species of Siphoviridae family, Vibrio phage Artemius, which can specifically infect V. alginolyticus strains, was reported. It efficiently reduces the growth of the host bacterium as assessed by an in vitro lysis assay.
Article
Fisheries
Xiongchao Ma, Fei Zhu
Summary: Myosin-9 plays a key role in the immune response of S. paramamosain against WSSV and V. alginolyticus, affecting the expression of immune-related genes and the antiviral capacity of crab hemocytes when knocked down.
Article
Environmental Sciences
Wenxiao Su, Yiqin Deng, Shujun Zang, Si'ao Gao, Juan Feng
Summary: Leucine-responsive regulatory protein (Lrp) plays a negative regulatory role in Vibrio alginolyticus, reducing its virulence by decreasing swarming motility, biofilm formation, extracellular protease secretion activity, and tolerance to copper ions. The expression of Lrp is influenced by various physicochemical factors.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Engineering, Marine
Zhen-Hao Liao, Huai-Ting Huang, Yu-Ru Lin, Bo-Ying Chen, Yi-Fan Lee, Yuan-Heng Lin, Chieh-Yuan Chuang, Fan-Hua Nan
Summary: This study aimed to investigate the effects of dietary natural feed supplement on the growth performance, non-specific responses, and disease resistance in Lates calcarifer. The results showed that the addition of product C improved the growth performance and immune parameters, and increased the resistance of Lates calcarifer to Vibrio alginolyticus.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Wenwen Zhang, Ruiqiang Xie, Xiaohua Douglas Zhang, Leo Tsz On Lee, Hongjie Zhang, Menghua Yang, Bo Peng, Jun Zheng
Article
Chemistry, Multidisciplinary
Shengke Li, Kit Ieng Kuok, Xia Ji, Anni Xu, Hang Yin, Jun Zheng, Huaping Tan, Ruibing Wang
Article
Immunology
Jin Zou, Si-Hoi Kou, Ruiqiang Xie, Michael S. VanNieuwenhze, Jiuxin Qu, Bo Peng, Jun Zheng
EMERGING MICROBES & INFECTIONS
(2020)
Article
Chemistry, Multidisciplinary
Shuwen Guo, Qiaoxian Huang, Yuan Chen, Jianwen Wei, Jun Zheng, Leyong Wang, Yitao Wang, Ruibing Wang
Summary: A novel guanidinium-functionalized pillar[5]arene, GP5, was designed and synthesized to effectively combat bacterial biofilms. GP5 showed high antibacterial potency against Gram-negative and Gram-positive strains and successfully disrupted preformed biofilms by penetrating biofilm barriers and destroying biofilm-enclosed bacteria. Additionally, host-guest complexation between GP5 and cefazolin sodium exhibited synergistic disruption activity against biofilms, providing a novel supramolecular platform for biofilm eradication.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Dai-xiao Yang, Hao Yang, Yun-chao Cao, Ming Jiang, Jun Zheng, Bo Peng
Summary: The study found that succinate is a crucial biomarker for phagocytosis in monocytes/macrophages, and exogenous succinate can enhance phagocytic rate, a process that is related to the TCA cycle. Furthermore, succinate also regulates gene expression associated with immune response and phagocytosis.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Immunology
Man-jun Yang, Ming Jiang, Xuan-xian Peng, Hui Li
Summary: This study investigated how water temperature affects the survival of tilapia to bacterial infection from the perspective of metabolic state. It was found that tilapia were more susceptible to infection at 33 degrees C, but the infection could be attenuated by exogenous myo-inositol. The exogenous myo-inositol inactivated the elevated TCA cycle, increasing the survival ability of tilapia at higher water temperature.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Xian-liang Zhao, Zhuang-gui Chen, Tian-ci Yang, Ming Jiang, Jie Wang, Zhi-xue Cheng, Man-jun Yang, Jia-xin Zhu, Tian-tuo Zhang, Hui Li, Bo Peng, Xuan-xian Peng
Summary: Exogenous glutamine can enhance the killing effect of antibiotics against multidrug-resistant bacteria, promote the influx of antibiotics, increase intracellular antibiotic concentrations, effectively prevent the development of antibiotic resistance, and provide new insights for the prevention or management of chronic, multidrug-resistant bacterial infections, bacterial persistence, and difficult-to-treat bacterial biofilms.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Article
Immunology
Yu-ming Peng, Jian-jun Tao, Su-fang Kuang, Ming Jiang, Xuan-xian Peng, Hui Li
Summary: This study identified potential polyvalent vaccine candidates against bacterial infections by constructing DNA vaccines from extracellular secretory proteins of Vibrio alginolyticus. Three of the vaccines showed cross-immune protective effects against various bacterial infections, indicating the possibility of using extracellular secretory proteome as an antigen pool for identifying cross-protective immunogens.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Chang Lu, Nian Zhang, Sihoi Kou, Liangliang Gao, Bo Peng, Yunlu Dai, Jun Zheng
Summary: In this research, sanguinarine was shown to act synergistically with various aminoglycosides, effectively potentiating their killing ability on diverse bacterial pathogens. Mechanistic studies revealed elevated intracellular ROS and DNA oxidative levels in cells treated with the combination of sanguinarine and aminoglycosides. Additionally, the presence of aminoglycosides promoted the uptake of sanguinarine and sanguinarine was shown to eliminate persister cells and established biofilm cells both in vivo and in vitro. This study provides a novel approach to reduce the clinical dosages of aminoglycosides.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jian-jun Tao, Juan-juan Xiang, Ming Jiang, Su-fang Kuang, Xuan-xian Peng, Hui Li
Summary: A modified microtitre plate dilution method, called the minimum killing concentration (MKC) test, was developed to evaluate the bactericidal efficacy of metabolites. The MKC test was suitable for different metabolites and clinically multidrug-resistant pathogens, and results correlated with the plate-counting test.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Microbiology
Ming Jiang, Zhuang-gui Chen, Hui Li, Tian-tuo Zhang, Man-jun Yang, Xuan-xian Peng, Bo Peng
Summary: Macrophages play a role in restricting bacterial infection by promoting phagocytosis and releasing cytokines and complement components. The expression of IL-1 beta and bacterial phagocytosis peak at 8 to 12 hours post-treatment, while the expression of complement component 3 continues to rise for 24 hours. Metabolomic analysis suggests a correlation between cellular concentrations of succinate and IL-1 beta, as well as inosine and C3. Inosine improves the survival of bacterial pathogen-infected mice and tilapia, indicating its potential therapeutic use in preventing and treating bacterial infections.
Article
Oncology
Xiao-li Yang, Peng Wang, Hua Ye, Ming Jiang, Yu-bin Su, Xuan-xian Peng, Hui Li, Jian-ying Zhang
Summary: Metabolomics is an efficient tool for screening biomarkers related to esophageal cancer. This study found that elevated amino acid metabolism and reduced carbohydrate metabolism are characteristic features of the disease. Metabolic differences were determined between different stages of the disease and between patients who received treatment and those who did not. Glycine, fructose, ornithine, and threonine were identified as potential biomarkers for evaluating disease prognosis and therapy in esophageal cancer.
FRONTIERS IN ONCOLOGY
(2022)
Article
Infectious Diseases
Ming Jiang, Xia Li, Chun-Lin Xie, Peng Chen, Wei Luo, Chu-xiao Lin, Qiao Wang, Ding-Ming Shu, Cheng-long Luo, Hao Qu, Jian Ji
Summary: Salmonella enterica is a food-borne pathogen that poses a severe threat to both poultry production and human health. Understanding antibiotic resistance mechanisms and developing novel control measures are essential in combating antibiotic-resistant S. enterica.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Multidisciplinary Sciences
Ming Jiang, Yu -bin Su, Jin-zhou Ye, Hui Li, Su -fang Kuang, Jia-han Wu, Shao-hua Li, Xuan-xian Peng, Bo Peng
Summary: The research reveals that as ampicillin-sensitive strains acquire resistance to ampicillin, the abundance of glucose progressively decreases. Ampicillin triggers the event by targeting pts promoter and pyruvate dehydrogenase, leading to increased glucose transport and inhibited glycolysis. This process results in the activation of pentose phosphate pathway, generating reactive oxygen species and causing genetic mutations. The competitive binding of accumulated pyruvate and ampicillin gradually restores pyruvate dehydrogenase activity, lowers glucose level, and activates cAMP/CRP complex, inhibiting glucose transport and reactive oxygen species but enhancing DNA repair, ultimately leading to ampicillin resistance. Glucose and Mn2+ delay the acquisition of resistance, providing a promising approach to control antibiotic resistance.
Article
Biochemistry & Molecular Biology
Ming Jiang, Xin-Hai Chen, Hui Li, Xuan-Xian Peng, Bo Peng
Summary: Multidrug-resistant bacteria pose a significant threat to public health. Through proteomic and metabolomic analyses, the study identified a correlation between L-Alanine metabolism and phagocytosis with mouse survival in Vibrio parahaemolyticus infection. Experimental validation showed that exogenous L-Alanine promoted phagocytosis of multidrug-resistant pathogens, revealing the underlying mechanism.