Article
Chemistry, Analytical
Juan Garcia-Bernalt Diego, Pedro Fernandez-Soto, Sergio Marquez-Sanchez, Daniel Santos Santos, Begona Febrer-Sendra, Beatriz Crego-Vicente, Juan Luis Munoz-Bellido, Moncef Belhassen-Garcia, Juan M. Corchado Rodriguez, Antonio Muro
Summary: Nucleic acid amplification diagnostics, although sensitive and specific, lack speed, robustness, affordability, and user-friendliness. This study combines loop-mediated isothermal amplification technology with a handheld portable device (SMART-LAMP) to enable real-time nucleic acid amplification reactions through simple colorimetric measurements. The device is controlled by a dedicated smartphone app and can be used for diagnosing different infectious diseases, including Schistosomiasis, Strongyloidiasis, and COVID-19. The combination of long-term stabilized LAMP master mixes and the SMART-LAMP device improves point-of-care diagnosis of infectious diseases in low-resource settings, and can be easily adapted for diagnosing other infectious diseases.
Article
Microbiology
Xu Chen, Cheng Du, Qiang Zhao, Qi Zhao, Yonghu Wan, Jun He, Wei Yuan
Summary: We reported a novel molecular diagnostic assay, RT-LAMP-AuNPs-LFA, for rapid and specific detection of HIV-1. The assay can be completed within 45 minutes, has a detection limit of 20 copies per test, and shows potential as a point-of-care diagnostic tool for HIV-1 detection in clinical settings.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Chemistry, Analytical
Ian T. Hull, Enos C. Kline, Gaurav K. Gulati, Jack Henry Kotnik, Nuttada Panpradist, Kamal G. Shah, Qin Wang, Lisa Frenkel, James Lai, Joanne Stekler, Barry R. Lutz
Summary: The study presents a novel quantitative detection method for HIV DNA or RNA using a combination of recombinase polymerase amplification and lateral flow detection technologies, allowing for rapid and accurate testing in low-resource healthcare settings, with the potential to improve the quality of HIV treatment.
ANALYTICAL CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Yaoqiang Shi, Lan Kang, Rongrong Mu, Min Xu, Xiaoqiong Duan, Yujia Li, Chunhui Yang, Jia-Wei Ding, Qinghua Wang, Shilin Li
Summary: Researchers have developed a novel diagnostic test using a combination of LAMP and CRISPR/Cas12a to detect S. flexneri infection quickly and reliably, with results visible to the naked eye in 40 minutes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Qiaoqiao Zhou, Jiangfei Pan, Laiming Mo, Zhaofan Luo, Zhifeng Qin, Zong Dai, Changqing Yi
Summary: This study developed a cost-effective, portable, and easy-to-use on-site genetic diagnostics sensing system for pathogen screening. The system consists of a paper-based biochip and a smartphone-based portable device, which together enable simultaneous detection of multiple pathogens and have demonstrated practicality in foodborne pathogen detection.
Review
Agriculture, Dairy & Animal Science
Alexandra Knox, Travis Beddoe
Summary: Equine viral diseases pose a significant threat to global health, with some viruses having zoonotic potential. Current diagnostic techniques are time-consuming, costly, and require laboratory conditions. New isothermal nucleic acid amplification technologies, such as LAMP and iiPCR, offer rapid results within an hour and potential for field deployment.
Article
Entomology
Yue Zhang, Weisong Li, Massimiliano Virgilio, Marc De Meyer, Zhihong Li
Summary: This study achieved rapid molecular identification of Ceratitis fruit flies using LAMP technology and mitochondrial genes as targets. A loop-mediated isothermal amplification identification technology system was established, which utilized rapid, rough DNA extraction and visual detection. The system demonstrated high sensitivity and simplicity, allowing the entire identification process to be completed within one hour.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Biophysics
Jayeon Song, Hyo Yong Kim, Soohyun Kim, Yujin Jung, Hyun Gyu Park
Summary: A novel technology, SP-HAMP, is described for target nucleic acid detection under isothermal conditions. By designing a unique hairpin probe, it is possible to detect target DNA with outstanding discrimination capability using only a single primer, without the need for complex multiple primers.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Jamin Ku, Khushbu Chauhan, Sang-Hyun Hwang, Yong-Joo Jeong, Dong-Eun Kim
Summary: Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification method that allows the simple, quick, and low-cost detection of various viral genes. However, LAMP assays are susceptible to generating non-specific amplicons. In this study, it was found that poly(ethylene glycol)-engrafted nanosized graphene oxide (PEG-nGO) can significantly enhance the specificity of LAMP by adsorbing surplus ssDNA primers, reducing non-specific annealing. The inclusion of PEG-nGO greatly improves the specificity and sensitivity of the LAMP assay, facilitating the detection of HCV-positive clinical samples with superior precision compared to RT-qPCR.
Article
Chemistry, Analytical
Huan-Huan Zhu, Yuan Li, Li-Xia Wu, Ke-Sheng Wang, Yue Zhang, Qiang-Yuan Fan, Zun-Zhen Ming, Wei-Qin Chen, Wei-Wei Liu
Summary: Loop-mediated isothermal amplification (LAMP) is a promising diagnostic tool for genetic amplification, known for its speed, simplicity, efficiency, and sensitivity. This research introduces an internal heating method for LAMP using magnetic nanoparticles (MNPs) called nano-LAMP, which can detect low concentrations of HPV and has been validated in clinical samples.
Article
Chemistry, Analytical
Naoki Uno, Ziyue Li, Lori Avery, Maroun M. Sfeir, Changchun Liu
Summary: CRISPR technology combined with isothermal amplification has been used as a powerful biosensing tool for sensitive nucleic acid detection. However, integrating isothermal amplification into CRISPR detection in a one-pot system has been challenging. In this study, a simple CRISPR gel biosensing platform was developed for HIV RNA detection, which successfully detected down to 30 copies of HIV RNA per test within 30 minutes.
ANALYTICA CHIMICA ACTA
(2023)
Review
Chemistry, Analytical
Jesse M. M. Marangoni, Kenneth K. S. Ng, Arezoo Emadi
Summary: Loop-mediated isothermal amplification (LAMP) is a useful tool for pathogen detection in clinical and environmental samples, but monitoring LAMP can be challenging for point-of-use applications. This review discusses the use of voltammetric sensors for LAMP monitoring and highlights various strategies for detecting DNA amplicons, pyrophosphate, and protons. The review also addresses the hurdles in adapting these sensors for on-site applications.
Review
Chemistry, Analytical
Bin Xiao, Ruiming Zhao, Nan Wang, Juan Zhang, Xiaoyun Sun, Ailiang Chen
Summary: Loop-mediated isothermal amplification (LAMP) is a widely used thermostatic nucleic acid amplification technology in clinical diagnosis and food safety detection. Centrifugal microfluidic chips, developed based on traditional microfluidic chips, utilize centrifugal force for liquid flow, mixing, and reaction, eliminating the need for complex valve and pump design. The combination of LAMP and centrifugal microfluidic chips holds great potential for rapid on-site nucleic acid detection.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Meng Chen, Qidi He, Yanli Tong, Zuanguang Chen
Summary: This study successfully combined LAMP with RCA to achieve rapid detection and quantification of pathogens. By using a strand displacement strategy in a single-tube reaction, different pathogen target genes were successfully detected, demonstrating the universality and reliability of the method.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Medical Laboratory Technology
Chang-Geun Park, Seohyeon Kim, Hyo-Sung Jeon, Seungwoo Han
Summary: The newly developed LAMP assay kit demonstrated good sensitivity, specificity, and reliability in detecting H pylori and 2143G and 2182C mutations in 23S rRNA. The study showed high agreement with traditional detection methods and sequencing analysis, making it a valid method for clinical detection of H pylori and point mutations.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2021)
Article
Biochemical Research Methods
Enrique Valera, Jacob Berger, Umer Hassan, Tanmay Ghonge, Julia Liu, Michael Rappleye, Jackson Winter, Daniel Abboud, Zeeshan Haidry, Ryan Healey, Na-Teng Hung, Nathaniel Leung, Naif Mansury, Alexander Hasnain, Christine Lannon, Zachary Price, Karen White, Rashid Bashir
Correction
Multidisciplinary Sciences
Ishan Taneja, Bobby Reddy, Gregory Damhorst, Sihai Dave Zhao, Umer Hassan, Zachary Price, Tor Jensen, Tanmay Ghonge, Manish Patel, Samuel Wachspress, Jackson Winter, Michael Rappleye, Gillian Smith, Ryan Healey, Muhammad Ajmal, Muhammad Khan, Jay Patel, Harsh Rawal, Raiya Sarwar, Sumeet Soni, Syed Anwaruddin, Benjamin Davis, James Kumar, Karen White, Rashid Bashir, Ruoqing Zhu
SCIENTIFIC REPORTS
(2019)
Article
Engineering, Biomedical
Jacob Berger, Enrique Valera, Aaron Jankelow, Carlos Garcia, Manik Akhand, John Heredia, Tanmay Ghonge, Cynthia Liu, Victor Font-Bartumeus, Gina Oshana, Justin Tiao, Rashid Bashir
BIOMEDICAL MICRODEVICES
(2020)
Article
Chemistry, Physical
Indrajit Srivastava, Muhammad S. Khan, Ketan Dighe, Maha Alafeef, Zhen Wang, Tuseeta Banerjee, Tanmay Ghonge, Lauren M. Grove, Rashid Bashir, Dipanjan Pan
Article
Immunology
Gregory L. Damhorst, Max W. Adelman, Michael H. Woodworth, Colleen S. Kraft
Summary: There is increasing recognition of the significance of the gut microbiome in human health and disease. Traditional and modern technologies have advanced our knowledge in this field, but standardizing the language and metrics for characterizing the microbiome is still a work in progress. The capabilities of gut microbiome-based diagnostics are promising for clinical applications, with selected examples demonstrating their potential impact.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Review
Infectious Diseases
Gregory L. Damhorst, Abigail Watts, Alfonso Hernandez-Romieu, Nonglin Mel, Melody Palmore, Ibne Karim M. Ali, Stewart G. Neill, Aley Kalapila, Jennifer R. Cope
Summary: This article presents a case of a patient with AIDS who developed Acanthamoeba castellanii encephalitis, a rare cause of central nervous system infection. It highlights the importance for clinicians to consider protozoal infections, specifically free-living amoeba, in immunocompromised patients with CNS infections refractory to traditional antimicrobial therapy.
LANCET INFECTIOUS DISEASES
(2022)
Article
Biochemical Research Methods
Morgan N. Greenleaf, Gregory L. Damhorst, David N. Ku, Eric J. Nehl, Erika A. Tyburski, Oliver Brand, Greg S. Martin, Wilbur A. Lam
Summary: The COVID-19 pandemic has highlighted the importance of point-of-care diagnosis for respiratory diseases, leading to the rise of microfluidic technology. Mesa Biotech has successfully developed the Accula platform for the diagnosis of various respiratory diseases, including SARS-CoV-2, and has subsequently been acquired by Thermo Fisher for a significant amount. The success of Mesa Biotech can be attributed to their innovative design and engineering choices, which have allowed them to transition from a lab-based product to a commercially successful platform.
Article
Immunology
Gregory L. Damhorst, Kari J. Broder, Elizabeth C. Overton, Ronelio Rara, Lindsay M. Busch, Eileen M. Burd, Andrew S. Webster, Colleen S. Kraft, Ahmed Babiker
Summary: This study retrospectively analyzed the application of Pneumocystis jirovecii PCR and C-T values in the diagnosis and management of Pneumocystis jirovecii pneumonia (PJP). The results showed that PCR testing had high sensitivity and specificity in detecting pathological infection, and the C-T values differed between patients with positive pathology results and those receiving treatment, with high accuracy in predicting positive pathology results.
OPEN FORUM INFECTIOUS DISEASES
(2022)
Article
Immunology
Hans P. Verkerke, Gregory L. Damhorst, Daniel S. Graciaa, Kaleb McLendon, William O'Sick, Chad Robichaux, Narayanaiah Cheedarla, Sindhu Potlapalli, Shang Chuen Wu, Kristin R. Harrington, Andrew Webster, Colleen Kraft, Christina A. Rostad, Jesse J. Waggoner, Neel R. Gandhi, Jeannette Guarner, Sara C. Auld, Andrew Neish, John D. Roback, Wilbur A. Lam, N. Sarita Shah, Sean R. Stowell
Summary: In this study, we evaluated SARS-CoV-2 nucleocapsid antigenemia as a marker of acute COVID-19 and investigated outliers to gain a better understanding of the phenomenon. The findings suggest that antigenemia can serve as a biomarker for acute SARS-CoV-2 infection and may have diagnostic utility in various contexts.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Immunology
Eli Wilber, Anne Piantadosi, Ahmed Babiker, Kaleb McLendon, William O'Sick, Eric Fitts, Andrew S. Webster, Hans Verkerke, James S. Kim, Varun K. Phadke, Nadine Rouphael, Boghuma K. Titanji, William T. Blake, Jessica Howard-Anderson, John D. Roback, Wilbur A. Lam, Gregory L. Damhorst
Summary: Immunocompromised patients with prolonged coronavirus disease 2019 symptoms present diagnostic and therapeutic challenges. Nucleocapsid antigenemia can serve as a marker for persistent infection and therapeutic response in these patients, especially those treated with anti-CD20 immunotherapy.
OPEN FORUM INFECTIOUS DISEASES
(2022)
Article
Computer Science, Hardware & Architecture
Dirk Pfaff, Shahaboddin Moazzeni, Leisheng Gao, Mei-Chen Chuang, Xin-Jie Wang, Chai Palusa, Robert Abbott, Rolando Ramirez, Maher Amer, Ming-Chieh Huang, Chih-Chang Lin, Fred Kuo, Wei-Li Chen, Tae Young Goh, Kenny Hsieh
IEEE SOLID-STATE CIRCUITS LETTERS
(2019)
Review
Engineering, Biomedical
Gregory L. Damhorst, Erika A. Tyburski, Oliver Brand, Greg S. Martin, Wilbur A. Lam
CURRENT OPINION IN BIOMEDICAL ENGINEERING
(2019)
Meeting Abstract
Chemistry, Multidisciplinary
Indrajit Srivastava, Muhammad Khan, Ketan Dighe, Tanmay Ghonge, Lauren Grove, Dipanjan Pan
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Article
Chemistry, Analytical
Tanmay Ghonge, Hatice Ceylan Koydemir, Enrique Valera, Jacob Berger, Carlos Garcia, Noshin Nawar, Justin Tiao, Gregory L. Damhorst, Anurup Ganguli, Umer Hassan, Aydogan Ozcan, Rashid Bashir