Article
Biochemical Research Methods
Qiaodong Wei, Ying Xiong, Yuhang Ma, Deyun Liu, Yunshu Lu, Shenghong Zhang, Xiaolong Wang, Huaxiong Huang, Yingbin Liu, Ming Dao, Xiaobo Gong
Summary: The physical and mechanical properties of red blood cells (RBCs) play an important role in disease diagnosis, but it is challenging to measure these properties accurately due to the unique shape and high heterogeneity of RBCs. In this study, a microfluidic technology combined with machine learning is developed to measure the mechanical properties of individual RBCs. The method can detect subtle changes in RBC components in response to different doses of drugs and explore the correlation between glycosylated hemoglobin and RBC mechanical properties. This approach has the potential to offer novel label-free single-cell-assay-based biophysical markers for RBCs, enhancing the potential for more robust disease diagnosis.
Article
Nanoscience & Nanotechnology
Yifan Liu, Fengxian Xin
Summary: A cyclic coupling computational model is developed to investigate the large deformation of swollen red blood cells induced by the acoustic radiation force. The model accurately predicts the acoustic deformability of cells by calculating the acoustic radiation force and mechanical response of the cell membrane. The results also demonstrate that the deformation of multiple cells is influenced by cell-cell interaction.
MICROFLUIDICS AND NANOFLUIDICS
(2022)
Article
Peripheral Vascular Disease
Francoise Steenebruggen, Daniel Jacobs, Cedric Delporte, Pierre Van Antwerpen, Karim Zouaoui Boudjeltia, Patrick Biston, Michael Piagnerelli
Summary: The deformability of red blood cells is affected by membrane damage and oxidative stress. Hyperbaric oxygen therapy can improve deformability and microcirculation in patients with inflammation.
MICROVASCULAR RESEARCH
(2023)
Article
Cell Biology
Adam Varga, Adam Attila Matrai, Barbara Barath, Adam Deak, Laszlo Horvath, Norbert Nemeth
Summary: Plasma and blood osmolality values vary among different species and are closely regulated. These factors also affect microrheological parameters, such as RBC deformability and aggregation. This study explored the interspecies differences in RBC deformability at various blood osmolality levels and found significant variation among species. The findings highlight the importance of osmotic gradient deformability in understanding microrheological diversity and alterations caused by osmolality changes.
Article
Multidisciplinary Sciences
Christina Caruso, Meredith E. Fay, Xiaopo Cheng, Alan Y. Liu, Sunita I. Park, Todd A. Sulchek, Michael D. Graham, Wilbur A. Lam
Summary: This study investigates the relationship between cardiovascular disease and iron deficiency anemia (IDA). It shows that subpopulations of IDA red blood cells (idRBCs) have different physical properties compared to healthy red blood cells, leading to their accumulation in the vessel wall and causing inflammation.
Article
Cell Biology
Claudia Bernecker, Eva Maria Matzhold, Dagmar Kolb, Afrim Avdili, Lisa Rohrhofer, Annika Lampl, Martin Troetzmueller, Heike Singer, Johannes Oldenburg, Peter Schlenke, Isabel Dorn
Summary: Functional characterization of cultured red blood cells from human induced pluripotent stem cells (cRBC_iPSCs) was conducted in this study. The results showed that cRBC_iPSCs have similar morphology and maturity stage to native red blood cells, highlighting their potential for future applications.
Article
Urology & Nephrology
Rosi Bissinger, Travis Nemkov, Angelo D'Alessandro, Marijke Grau, Thomas Dietz, Bernhard N. Bohnert, Daniel Essigke, Matthias Woern, Lina Schaefer, Mengyun Xiao, Jonathan M. Beirne, M. Zaher Kalo, Anja Schork, Tamam Bakchoul, Kingsley Omage, Lingsi Kong, Irene Gonzalez-Menendez, Leticia Quintanilla-Martinez, Birgit Fehrenbacher, Martin Schaller, Achal Dhariwal, Andreas L. Birkenfeld, Florian Grahammer, Syed M. Qadri, Ferruh Artunc
Summary: This study using mouse models demonstrates the development of chronic kidney disease-associated anemia is linked to altered red blood cell metabolism, reduced RBC deformability, and enhanced cell death.
KIDNEY INTERNATIONAL
(2021)
Review
Physiology
Gregory Barshtein, Ivana Pajic-Lijakovic, Alexander Gural
Summary: Red blood cells' deformability plays a crucial role in adapting to flow conditions, with high deformability facilitating flow through small blood vessels. Loss of deformability may lead to cell rigidity and dysfunction.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Travis Nemkov, Sarah C. Skinner, Elie Nader, Davide Stefanoni, Melanie Robert, Francesca Cendali, Emeric Stauffer, Agnes Cibiel, Camille Boisson, Philippe Connes, Angelo D'Alessandro
Summary: A controlled, high-intensity cycling test resulted in decreased RBC deformability, increased microparticle generation, oxidative stress, and activation of membrane lipid remodeling mechanisms. Metabolites involved in CoA synthesis and carnitine conversion were significantly correlated with RBC properties during exercise.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Brenda J. Green, Margherita Marazzini, Ben Hershey, Amir Fardin, Qingsen Li, Zongjie Wang, Giovanni Giangreco, Federica Pisati, Stefano Marchesi, Andrea Disanza, Emanuela Frittoli, Emanuele Martini, Serena Magni, Galina Beznoussenko, Claudio Vernieri, Riccardo Lobefaro, Dario Parazzoli, Paolo Maiuri, Kristina Havas, Mahmoud Labib, Sara Sigismund, Pier Paolo Di Fiore, Rosalind H. Gunby, Shana O. Kelley, Giorgio Scita
Summary: The PillarX device is a bimodular microfluidic device that can analyze circulating tumor cell (CTC) clusters based on their size, deformability, and epithelial marker expression. It captures and separates clusters based on their size and deformability, and can analyze clusters driven by different cell-cell adhesion protein expression. The device has been validated in mouse models and patients of metastatic breast cancer.
Article
Biology
Steffen M. Recktenwald, Greta Simionato, Marcelle G. M. Lopes, Fabia Gamboni, Monika Dzieciatkowska, Patrick Meybohm, Kai Zacharowski, Andreas von Knethen, Christian Wagner, Lars Kaestner, Angelo D'Alessandro, Stephan Quint
Summary: The flow properties and shape of red blood cells in COVID-19 patients are significantly impaired, but these changes are reversible when suspended in healthy plasma. The study also reveals a new role of red blood cells in maintaining plasma equilibrium. These findings provide a framework for further investigations into the treatment of COVID-19 and other infectious diseases.
Article
Chemistry, Analytical
Roya Mohammadali, Morteza Bayareh
Summary: This paper introduces a method of isolating deformable CTCs using inertial-based spiral microchannels and discusses the effects of cell deformability, cell size, number of loops, and channel depth on the hydrodynamic behavior of CTCs. The results demonstrate that this method can be used for sorting and isolating label-free deformable cells at large scales in clinical systems.
Review
Chemistry, Analytical
Georgii V. V. Grigorev, Alexander V. V. Lebedev, Xiaohao Wang, Xiang Qian, George V. V. Maksimov, Liwei Lin
Summary: The utilization of microfluidic chips for single RBC studies has attracted great interest in recent years. It involves filtering, trapping, analyzing, and releasing single erythrocytes for various applications. This article critically reviews the current state-of-the-art and recent advances in microfluidics for single RBC analyses, including integrated sensors, trapping arrays, dielectrophoretic and agglutination/aggregation studies, clinical implications, microarrays, sorting/counting and trapping techniques, organs-on-chips, multi-organ chips, and drug discovery. The limitations and future prospects of each technology are also addressed.
Review
Cell Biology
Neslihan Cilek, Elif Ugurel, Evrim Goksel, Ozlem Yalcin
Summary: Intracellular signaling mechanisms in red blood cells (RBCs) involve protein kinases and phosphatases, which regulate the physiological properties of the cell in response to various stimuli. Protein phosphorylation plays a crucial role in intracellular signal transduction, volume regulation, and cytoskeletal organization in RBCs. The structural organization of the membrane and cytoskeleton determines RBC deformability and allows them to pass through narrow capillaries under shear stress.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Hossein Tavassoli, Prunella Rorimpandey, Young Chan Kang, Michael Carnell, Chris Brownlee, John E. Pimanda, Peggy P. Y. Chan, Vashe Chandrakanthan
Summary: A label-free method using inertial microfluidics is developed to purify viable cardiomyocytes from mouse neonatal hearts with purities over 90%. The isolated cardiomyocytes retain their identity and function, exhibiting different physico-mechanical properties compared to non-cardiomyocytes. This method could be a valuable tool for advancing the understanding of cardiomyocyte identity and function, with potential benefits for cell therapy and diagnostic applications.
Article
Biophysics
Amit K. Saha, Shatha F. Dallo, Ariana L. Detmar, Pawel Osmulski, Maria Gaczynska, Tim Hui-Ming Huang, Anand K. Ramasubramanian
JOURNAL OF BIOMECHANICS
(2017)
Article
Cell Biology
Amit K. Saha, Marzieh Mousavi, Shatha F. Dallo, Shankar J. Evani, Anand K. Ramasubramanian
CELLULAR IMMUNOLOGY
(2018)
Article
Multidisciplinary Sciences
Soutik Betal, Amit Kumar Saha, Eduardo Ortega, Moumita Dutta, Anand Kumar Ramasubramanian, Amar Singh Bhalla, Ruyan Guo
SCIENTIFIC REPORTS
(2018)
Meeting Abstract
Biophysics
Amit K. Saha, Brendan R. Schmidt, Julie Wilhelmy, Vy Nguyen, Justin K. Do, Vineeth C. Suja, Mohsen Nemat-Gorgani, Anand K. Ramasubramanian, Ronald W. Davis
BIOPHYSICAL JOURNAL
(2019)
Article
Biophysics
Sang-Joon J. Lee, Dustin M. Nguyen, Harjot S. Grewal, Chaitanya Puligundla, Amit K. Saha, Prajeeda M. Nair, Andrew P. Cap, Anand K. Ramasubramanian
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2020)
Article
Hematology
Amit K. Saha, Min-Yi S. Zhen, Folarin Erogbogbo, Anand K. Ramasubramanian
SEMINARS IN THROMBOSIS AND HEMOSTASIS
(2020)
Article
Multidisciplinary Sciences
Poonam Agarwal, Abhinav Kaushik, Sutapa Sarkar, Deepti Rao, Nilanjan Mukherjee, Vinita Bharat, Subhamoy Das, Amit Kumar Saha
Summary: The COVID-19 pandemic has significantly impacted individuals' socio-economic and health conditions, with increased virtual interaction, focus on physical fitness, and majority living with companions. Unemployment did not affect most survey participants, and coping mechanisms included increased technology-aided distant social interaction and engagement in physical activities during home isolation.
Meeting Abstract
Hematology
Amit K. Saha, Brendan R. Schmidt, Julie Wilhelmy, Vy Nguyen, Justin Do, Vineeth C. Suja, Mohsen Nemat-Gorgani, Anand K. Ramasubramanian, Ronald W. Davis
Meeting Abstract
Hematology
Wilson Eng, Max Kim, Stephanie Pham, Amit Saha, Anand K. Ramasubramanian, Sang-Joon Lee
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2018)
Article
Biophysics
Amit K. Saha, Pawel Osmulski, Shatha F. Dallo, Maria Gaczynska, Tim H. -M. Huang, Anand K. Ramasubramanian
BIOPHYSICAL JOURNAL
(2017)