Article
Veterinary Sciences
Yeon Chae, Taesik Yun, Yoonhoi Koo, Dohee Lee, Hakhyun Kim, Mhan-Pyo Yang, Byeong-Teck Kang
Summary: This study used FDG-PET/CT to identify physiological FDG uptake in cats and compared it with dogs, revealing differences in brain and digestive system uptake. Understanding these differences can improve cancer diagnosis and glucose metabolism understanding in both cats and dogs.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Biochemical Research Methods
Filipa Mota, Patricia De Jesus, Sanjay K. Jain
Summary: Researchers developed a method to synthesize F-18-FDS for positron emission tomography imaging to visualize infections. Clinical study in 26 patients showed that F-18-FDS PET was safe and could differentiate infections caused by Enterobacterales from other pathologies such as sterile inflammation or cancer.
Article
Pharmacology & Pharmacy
Gaelle Hugon, Sebastien Goutal, Ambre Dauba, Louise Breuil, Benoit Larrat, Alexandra Winkeler, Anthony Novell, Nicolas Tournier
Summary: Focused ultrasound in combination with microbubbles enhances therapeutics delivery to the brain by increasing blood-brain barrier permeability. PET imaging using [F-18]FDS is evaluated as a marker for BBB integrity, showing significantly increased distribution in the brain after FUS. These findings demonstrate the potential of [F-18]FDS PET as a sensitive and reproducible marker for noninvasive monitoring of BBB disruption induced by FUS in vivo.
Article
Chemistry, Organic
Timaeus E. F. Morgan, Leanne M. Riley, Adriana A. S. Tavares, Andrew Sutherland
Summary: This study developed two synthetic routes that allow highly selective radiofluorination reactions and rapid deprotection of precursors under mild acidic conditions, improving the synthesis of positron emission tomography imaging agents.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Oncology
Maxwell W. G. Miner, Heidi Liljenback, Jenni Virta, Semi Helin, Olli Eskola, Petri Elo, Jarmo Teuho, Kerttu Seppala, Vesa Oikonen, Guangli Yang, Andrea Kindler-Roehrborn, Heikki Minn, Xiang-Guo Li, Anne Roivainen
Summary: This study investigated the imaging capabilities of three PET imaging compounds in orthotopic BT4C gliomas in BDIX rats and compared two imaging systems. Results indicated that [F-18]FGln may offer improved imaging compared to [C-11]Met and [F-18]FDG, with similar end-result ratios between Inveon and Molecubes camera systems. Pharmacokinetic modeling suggested both reversible and irreversible uptake play an important role in the uptake of [F-18]FGln in BDIX rat pharmacokinetics.
FRONTIERS IN ONCOLOGY
(2021)
Article
Chemistry, Inorganic & Nuclear
Junling Li, Huaiyu Zheng, Alexis A. Vega, Levi J. Beverly, Brian D. Gray, Koon Y. Pak, Chin K. Ng
Summary: [18F]FGA was obtained from [18F]FDG through one-step oxidation using sodium hypochlorite, with a confirmed conversion rate of over 95% by HPLC. The tumor uptake of [18F]FGA showed no significant difference in all mice before saline or cisplatin treatment, with an average uptake of (0.21 ± 0.16) %ID/g. However, after treatment, the tumor uptake of [18F]FGA significantly increased in cisplatin-treated mice, with an average uptake of (1.63 ± 0.16) %ID/g, and the ratio of tumor uptake between cisplatin-treated vs. saline-treated mice was 7.8 ± 0.2 within one week of treatment. PET imaging results were consistent with ex vivo biodistribution data, and BLI showed significant light intensity suppression after treatment, indicating necrosis. These findings suggest that [18F]FGA PET/CT imaging could be a useful tool for monitoring chemotherapy response by imaging tumor necrosis.
APPLIED RADIATION AND ISOTOPES
(2023)
Article
Biochemistry & Molecular Biology
Xiang Zhang, Falguni Basuli, Sameh Abdelwahed, Tadhg Begley, Rolf Swenson
Summary: A simple and efficient process for fluorine-18-labeled 1,2,3-triazole was reported, achieving successful radiofluorination through iodine-fluorine-18 exchange in an aqueous medium. The methodology was optimized on a model reaction and validated on multiple 1,2,3-triazole substrates, with radiochemical conversions ranging from 18-60%. Using this strategy, a triazole-based thiamin analogue was synthesized with isolated radiochemical yield of 10-16% in 40 minutes, showing potential for positron emission tomography (PET) imaging of thiamin-dependent enzymes.
Article
Biochemistry & Molecular Biology
Amanda J. Boyle, Emily Murrell, Junchao Tong, Christin Schifani, Andrea Narvaez, Melinda Wuest, Frederick West, Frank Wuest, Neil Vasdev
Summary: This study demonstrates the potential of using fluorine-18 labeled 6-fluoro-6-deoxy-D-fructose (6-[F-18]FDF) as a PET radiotracer to image microglial GLUT5 density in the brain following neuroinflammatory insult. The uptake of 6-[F-18]FDF in the ipsilateral striatum of LPS-injected rats was significantly higher compared to the contralateral striatum, while [F-18]FDG showed highest uptake at one week post-LPS injection. Immunohistochemistry confirmed the activation of microglia and astrocytes in the ipsilateral striatum. This proof-of-concept study highlights the early response of 6-[F-18]FDF to neuroinflammatory stimuli and its potential applications in neuroinflammatory and neurodegenerative diseases.
Article
Radiology, Nuclear Medicine & Medical Imaging
Antonio Canosa, Alessio Martino, Umberto Manera, Rosario Vasta, Maurizio Grassano, Francesca Palumbo, Sara Cabras, Francesca Di Pede, Vincenzo Arena, Cristina Moglia, Alessandro Giuliani, Andrea Calvo, Adriano Chio, Marco Pagani
Summary: This study aimed to evaluate the accuracy of brain 2-[F-18]fluoro-2-deoxy-D-glucose-positron-emission tomography (2-[F-18]FDG-PET) as a predictor of survival in patients with amyotrophic lateral sclerosis (ALS). The results showed that brain 2-[F-18]FDG-PET, combined with artificial intelligence, could accurately predict the survival time range in ALS patients. This prognostic tool has potential applications in patient management and individual stratification in clinical trials.
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2023)
Article
Chemistry, Physical
Paul M. DeMent, Chenlu Liu, Joseph Wakpal, Richard N. Schaugaard, H. Bernhard Schlegel, Hien M. Nguyen
Summary: Phenanthroline is a commonly used bidentate ligand in coordination chemistry, which can be tuned to develop efficient and stereoselective catalysts. By activating 2-deoxy-2-fluoro glycosyl halides with phenanthroline-based catalysts, glycosylations with high levels of alpha-selectivities and moderate to high yields can be achieved. The proposed mechanism for this catalytic glycosylation system involves double S(N)2 displacement pathways with phenanthroline catalysts, ensuring stereoselective formation of alpha-1,2-cis-2-fluoro glycosides.
Article
Chemistry, Organic
Yuliya Dantsu, Ying Zhang, Wen Zhang
Summary: In this study, a chemically derivatized 2'-deoxy-2'-fluoro-L-uridine building block was synthesized and incorporated into oligonucleotides. Thermo-denaturization and enzymatic digestion experiments showed superior stability of the modified molecules. The crystal structure of L-type fluoro-DNA was determined, revealing increased L-helix stability through fluoro-modification and laying the foundation for potential functional applications.
Article
Radiology, Nuclear Medicine & Medical Imaging
Hongliang Wang, Qinan Zhao, Weixuan Dong, Liu Yang, Keyi Lu, Xiaoshan Guo, Haiyan Liu, Hua Wei, Yan Cheng, Zhifang Wu, Sijin Li
Summary: The study successfully prepared an ornithine derivative, N-5-[F-18]FPO, and evaluated its potential for tumor PET imaging. The results showed that N-5-[F-18]FPO has high radiochemical stability, fast uptake in tumor cells, and excellent tumor visualization in tumor-bearing mouse models. This suggests that N-5-[F-18]FPO has the potential to be a novel radiotracer for imaging ODC expression in solid tumors.
NUCLEAR MEDICINE AND BIOLOGY
(2021)
Article
Imaging Science & Photographic Technology
Xuewei Mao, Wei Shan, Wilson Fox, Jinpeng Yu
Summary: In this paper, a computer-aided image subtraction technique was proposed to overcome the challenge of extracting positron emission tomography (PET) features in patients with neurological disorders. The technique effectively eliminated the metabolic interference of background issues and highlighted lesions. The study validated the feasibility of the technique by analyzing PET data before and after treatment in patients with anti-leucine-rich glioma-inactivated 1 (LGI1) encephalitis, demonstrating successful extraction of relevant brain regions and identification of metabolic changes.
IMAGING SCIENCE JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Niklas Kolks, Felix Neumaier, Bernd Neumaier, Boris D. Zlatopolskiy
Summary: Tryptophan is a crucial amino acid involved in various physiological and pathological processes. This study presents the synthesis of two fluorinated tryptophan derivatives and their potential as imaging agents for pathway-specific PET imaging.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Lisa J. States, J. Christopher Davis, Steven M. Hamel, Susan A. Becker, Hongming Zhuang
Summary: Congenital hyperinsulinism is a condition characterized by persistent hypoglycemia due to excessive insulin secretion, with varying clinical course and potential cure through surgical resection of focal lesions.F-18-6-fluoro-L-dopa PET/CT plays a vital role in detection and management of this condition.
JOURNAL OF NUCLEAR MEDICINE
(2021)
Review
Chemistry, Multidisciplinary
Nicholas Banahene, Herbert W. Kavunja, Benjamin M. Swarts
Summary: This article reviews the physiological functions of bacterial glycans and the development and applications of chemical reporters targeting bacterial glycans, including their use in diagnostics, therapeutics, and research.
Review
Multidisciplinary Sciences
Blanca Comitre-Mariano, Magdalena Martinez-Garcia, Barbara Garcia-Galvez, Maria Paternina-Die, Manuel Desco, Susanna Carmona, Maria Victoria Gomez-Gaviro
Summary: Fetal microchimerism (FMc) refers to the presence and persistence of fetal cells in maternal tissues. FMc has high multilineage potential, can integrate into maternal tissues, and has functional effects. Studying the existence and characteristics of FMc in maternal tissues is crucial for understanding its impact on women's health.
Article
Chemistry, Multidisciplinary
Albert Belenguer-Llorens, Carlos Sevilla-Salcedo, Manuel Desco, Maria Luisa Soto-Montenegro, Vanessa Gomez-Verdejo
Summary: In this paper, a novel machine learning model based on Bayesian linear regression is proposed to address the low sample-to-variable ratio common in neuroimaging studies of mental disorders. The model combines feature selection capabilities with a formulation in the dual space to effectively handle neuroimaging data. The proposed algorithm is tested on real MRI data from an animal model of schizophrenia, and the results demonstrate efficient diagnosis prediction and detection of brain areas associated with schizophrenia.
APPLIED SCIENCES-BASEL
(2022)
Article
Chemistry, Multidisciplinary
Nicholas Banahene, Dana M. Gepford, Kyle J. Biegas, Daniel H. Swanson, Yen-Pang Hsu, Brennan A. Murphy, Zachary E. Taylor, Irene Lepori, M. Sloan Siegrist, Andres Obregon-Henao, Michael S. Van Nieuwenhze, Benjamin M. Swarts
Summary: This study presents a trehalose-based fluorogenic probe, RMR-Tre, which exploits the enzymes and environment of the mycobacterial outer membrane to achieve fluorescence activation. RMR-Tre enables fast, no-wash, low-background fluorescence detection of live mycobacteria. Compared to existing probes, RMR-Tre exhibits a 100-fold enhancement in M. tuberculosis labeling and has the potential for TB diagnostic tool.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Correction
Chemistry, Medicinal
Amol Arunrao Pohane, Devin J. Moore, Irene Lepori, Rebecca A. Gordon, Temitope O. Nathan, Dana M. Gepford, Herbert W. Kavunja, V. Ishani Gaidhane, Benjamin M. Swarts, M. Sloan Siegrist
ACS INFECTIOUS DISEASES
(2023)
Article
Biochemistry & Molecular Biology
Priscilla Dzigba, Adrian K. K. Rylski, Isaac J. J. Angera, Nicholas Banahene, Herbert W. W. Kavunja, Mallary C. C. Greenlee-Wacker, Benjamin M. M. Swarts
Summary: Mycobacterial cell envelope intrinsic drug tolerance undermines conventional antibiotic treatment, but a novel strategy has been developed to enhance the host immune response by specifically decorating the cell surface glycans with antibody-recruiting molecules (ARMs). This strategy was shown to be effective in recruiting anti-DNP antibodies to the mycobacterial cell surface, enhancing phagocytosis by macrophages.
ACS CHEMICAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Diego Romero-Miguel, Marta Casquero-Veiga, Javier Fernandez, Nicolas Lamanna-Rama, Vanessa Gomez-Rangel, Carlos Galvez-Robleno, Cristina Santa-Marta, Claudio J. Villar, Felipe Lombo, Raquel Abalo, Manuel Desco, Maria Luisa Soto-Montenegro
Summary: N-acetylcysteine (NAC) administration during pregnancy can prevent inflammation-induced brain changes and improve memory deficits in offspring. This suggests a potential role of NAC in modulating the microbiota-gut-brain axis.
Article
Microbiology
J. J. Aguilera-Correa, B. Salinas, M. Gonzalez-Arjona, D. de Pablo, P. Munoz, E. Bouza, M. J. Fernandez Acenero, J. Esteban, M. Desco, L. Cusso
Summary: This study compared structural and molecular imaging techniques to assess disease progression in a mouse model of osteomyelitis. The results showed that FDG-PET imaging was more sensitive than MRI and CT in differentiating between infection and inflammation at early stages.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biotechnology & Applied Microbiology
Maria Isabel Gonzalez, Begona Gallardo, Carlos Ceron, Elena Aguilera-Jimenez, Marta Cortes-Canteli, Hector Peinado, Manuel Desco, Beatriz Salinas
Summary: This study presents an optimized approach for purifying exosomes from goat milk by combining physical isolation methods with biological treatment. The vesicles isolated with this novel strategy showed characteristics similar to exosomes and high homogeneity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
F. Arias-Valcayo, P. Galve, Joaquin L. Herraiz, J. J. Vaquero, M. Desco, J. M. Udias
Summary: In this study, a spatially-variant resolution model was incorporated into a real time iterative reconstruction code for accurate imaging of multi-animal PET studies. The spatially variant point spread function (SV-PSF) was estimated using measurements and Monte Carlo simulations, and implemented in a GPU-based reconstruction code. The method showed uniform spatial resolution and superior image quality compared to the uniform PSF model.
BIOMEDICAL PHYSICS & ENGINEERING EXPRESS
(2023)
Article
Chemistry, Multidisciplinary
Fernando Arias-Valcayo, Pablo Galve, Jose Manuel Udias, Juan Jose Vaquero, Manuel Desco, Joaquin L. Herraiz
Summary: This study investigates the optimal conditions and motion compensation methods for preclinical PET studies using awake rats with attached point sources. Monte Carlo simulations and experimental validations are performed to determine the parameters for maximum detectability without compromising image quality.
APPLIED SCIENCES-BASEL
(2023)
Article
Chemistry, Multidisciplinary
Ishani V. Gaidhane, Kyle J. Biegas, Helen E. Erickson, Prachi Agarwal, Yashpal S. Chhonker, Donald R. Ronning, Benjamin M. Swarts
Summary: Synthetic lipids can remodel the outer membrane of Mycobacterium, enhancing cellular permeability and sensitizing bacteria to antibiotics.
CHEMICAL COMMUNICATIONS
(2023)