Article
Plant Sciences
Muhammad Azhar Hussain, Senquan Li, Hongtao Gao, Chen Feng, Pengyu Sun, Xiangpeng Sui, Yan Jing, Keheng Xu, Yonggang Zhou, Wenping Zhang, Haiyan Li
Summary: In this study, a diverse genetic panel of soybean varieties was analyzed, leading to the identification of cold tolerant (V100) and cold sensitive (V45) varieties based on photosynthesis, chlorophyll fluorescence, and leaf injury parameters. The V100 variety showed reduced accumulation of H2O2 and MDA levels under cold stress, resulting in lower leaf injury compared to V45.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Guodong Ge, Yong Long, Guili Song, Qing Li, Zongbin Cui, Huawei Yan
Summary: Temperature has a significant impact on the reproduction of zebrafish, with lower temperatures inhibiting the spawning of females. Through RNA-seq analysis of brain tissues under different cold temperatures, key genes, hub pathways, and important biological processes involved in the spawning of zebrafish were identified. The findings highlight hormone-related genes and signaling pathways crucial for the spawning of female zebrafish under cold stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Chunyu Zhang, Chengyu Zhang, Xiaoqiong Xu, Minzhang Liao, Ning Tong, Zihao Zhang, Yukun Chen, Xu Xu Han, Yuling Lin, Zhongxiong Lai
Summary: Our study demonstrates that the balance between various metabolic processes, pollen wall remodelling, DNA methylation, DNA repairs and the regulation of MYBs are essential for germination of wild banana pollens.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Madiha Zaynab, Jiaofeng Peng, Yasir Sharif, Mahpara Fatima, Mohammed Albaqami, Rashid Al-Yahyai, Ali Raza, Khalid Ali Khan, Saqer S. Alotaibi, Ibrahim A. Alaraidh, Hassan O. Shaikhaldein, Shuangfei Li
Summary: In this study, a comprehensive analysis of the potato genome identified 70 StGLPs genes and revealed their expression patterns under salt and heat stress. These findings provide a foundation for further understanding the mechanisms of potato's response to these stresses.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Genetics & Heredity
Melanie Mazurier, Jan Drouaud, Nasser Bahrman, Andrea Rau, Isabelle Lejeune-Henaut, Bruno Delbreil, Sylvain Legrand
Summary: This study used time series sRNA and mRNA sequencing experiments to identify the miRNA families and target genes involved in cold stress response in pea. These findings provide insights into the physiological response mechanisms of plants under cold conditions and have important implications.
Article
Biotechnology & Applied Microbiology
Chenggang Wang, Mengyun Zhang, Jiajie Zhou, Xun Gao, Shidong Zhu, Lingyun Yuan, Xilin Hou, Tongkun Liu, Guohu Chen, Xiaoyan Tang, Guolei Shan, Jinfeng Hou
Summary: The study revealed that starch and sucrose metabolism, as well as glutathione metabolism, may play roles in response to cold stress in Wucai, while the activation of alpha-linolenic acid metabolism may promote the synthesis of methyl jasmonate (MeJA) to enhance cold tolerance. These findings provide insights into the molecular mechanisms underlying cold tolerance in Wucai.
Article
Agricultural Engineering
Xiaoyu Wang, Ying Wu, Mengdi Sun, Xue Wei, Hongyan Huo, Lili Yu, Jixing Zhang
Summary: In this study, the transcriptome changes of a cold-tolerant castor variety, Tongbi 5, under different cold treatments were analyzed using RNA-Seq. The results showed that a large number of genes were differentially expressed after cold stress, with the upregulated genes being more abundant. Functional analysis revealed the involvement of these differentially expressed genes in nuclear processes, transcription regulation, kinase cascades, plant circadian rhythm, and other functions. A trade-off model between plant growth and cold tolerance in castor was proposed.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Plant Sciences
Chaw Su Su Htwe, Subramani Rajkumar, Pooja Pathania, Anuradha Agrawal
Summary: This study investigated the cryopreservation mechanism of banana through transcriptomic analysis and identified differentially expressed genes at different stages, providing a basis for developing an effective cryopreservation protocol.
Article
Biotechnology & Applied Microbiology
Muchun Niu, Wenjian Cao, Yongcheng Wang, Qiangyuan Zhu, Jiayi Luo, Baiping Wang, Hui Zheng, David A. Weitz, Chenghang Zong
Summary: The profiling of individual synapses reveals molecular heterogeneity, which is crucial for signal transmission and shows considerable morphological and electrophysiological heterogeneity. However, there is currently a lack of high-throughput methods to analyze the molecular heterogeneity among individual synapses.
NATURE BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Tao Yan, Meng Sun, Rui Su, Xiaozhong Wang, Xuedan Lu, Yunhua Xiao, Huabing Deng, Xiong Liu, Wenbang Tang, Guilian Zhang
Summary: Cold stress has a significant impact on the growth and development of rice, particularly the susceptible indica cultivar. This study identified genes and transcription factors associated with cold responsiveness in indica rice and revealed the molecular mechanisms underlying cold responses, providing important insights for enhancing cold tolerance in susceptible varieties.
Article
Biochemical Research Methods
Aman Chandra Kaushik, Qiqi Wu, Li Lin, Haibo Li, Longqi Zhao, Zilu Wen, Yanzheng Song, Qihang Wu, Jin Wang, Xiaokui Guo, Hualin Wang, Xiaoli Yu, Dongqing Wei, Shulin Zhang
Summary: The study indicates that exosomal ncRNAs may serve as useful functional biomarkers in tuberculosis, with miR-185-5p identified as a potential biomarker for tuberculosis.
BRIEFINGS IN BIOINFORMATICS
(2021)
Article
Biochemistry & Molecular Biology
Zhiming Han, Xiangzhu Xu, Shikai Zhang, Qiushuang Zhao, Hanxi Li, Ying Cui, Xiao Li, Liran Wang, Su Chen, Xiyang Zhao
Summary: This study analyzed the physiological indicators and transcriptome of Acer pseudosieboldianum under low temperature stress. The results showed the presence of thousands of differential genes at different temperatures and the activation of important transcription factors related to freezing stress. In addition, most of the physiological indicators reached their maximum values at -30 degrees Celsius.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Tianjiu Zhang, Xiaolin Hu, Song Yu, Chunyan Wei
Summary: In this study, the expression patterns of lncRNAs, miRNAs, and mRNAs in a rabbit model of Perthes disease were analyzed using transcriptome sequencing. The results indicated that multiple genetic pathways are involved in the development of Perthes disease. Network analysis revealed downregulation of genes associated with angiogenesis and platelet activation, consistent with the characteristics of Perthes disease. Furthermore, a ceRNA network was constructed, including differentially expressed lncRNAs, miRNAs, and mRNAs.
FRONTIERS IN GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Guangchao Liu, Fuxia Liu, Yue Wang, Xin Liu
Summary: With the increasing global warming, cold stress has become a major factor restricting global crop yield. A novel lncRNA called CIL1 was identified as a positive regulator of the plant response to cold stress in Arabidopsis. CIL1 enhances the plant's resistance to cold stress by regulating the expression of multiple stress-related genes.
Article
Health Care Sciences & Services
Pritmohinder S. Gill, Harsh Dweep, Shannon Rose, Priyankara J. Wickramasinghe, Kanan K. Vyas, Sandra McCullough, Patricia A. Porter-Gill, Richard E. Frye
Summary: This study analyzed RNA and miRNA sequencing profiles of lymphoblastoid cell lines from children diagnosed with autistic disorder and controls, and identified numerous differentially expressed genes and miRNAs between the two groups. Functional enrichment analysis revealed critical pathways involved in ASD, and the integration analysis showed dysregulated miRNA-gene pairs. These findings suggest altered mRNA and miRNA expression profiles in ASD and provide insights into the regulatory mechanisms of key pathways.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Horticulture
David M. Bodjrenou, Chunzhen Cheng, Xueli Sun, Huifang Chew, Yanying Liu, Dan Li, Yankey Richard, Zhongxiong Lai
Summary: Our study revealed the important regulatory roles of miRNA in banana heat tolerance, particularly by regulating genes involved in heat shock protein, peroxidase, transcription factor, phenylalanine, sucrose synthase, protein kinase, and 7-hydroxymethyl chlorophyll. This provides a framework for future functional analysis of selected miRNAs and their targets under heat stress conditions, potentially contributing to the application of S. indica in plants in the future.
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
(2022)
Editorial Material
Plant Sciences
Yongyan Zhang, Fan Liu, Bin Wang, Dongliang Qiu, Jiapeng Liu, Huan Wu, Chunzhen Cheng, Xuejun Bei, Peitao Lu
Article
Biochemistry & Molecular Biology
Chunzhen Cheng, Fan Liu, Xueli Sun, Bin Wang, Jiapeng Liu, Xueting Ni, Chunhua Hu, Guiming Deng, Zheng Tong, Yongyan Zhang, Peitao Lu
Summary: Fatty acid desaturase (FAD) plays important roles in plant growth, development, and defense processes. In this study, we identified and classified 27 MaFAD genes into five subfamilies based on amino acid sequence similarities. The expansion of the MaFAD gene family is mainly attributed to segmental duplication events. We also discovered miRNAs targeting MaFADs and various regulatory elements in their promoters, indicating the complex regulation of MaFAD expression. Furthermore, gene expression analysis revealed significant differential expression of MaFADs under high and low temperature, as well as the influence of FocTR4 and S. indica on MaFAD expression and fatty acid accumulation in banana roots. Overall, our study highlights the importance of MaFADs in banana's response to temperature stress and fungal colonization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Horticulture
Huan Wu, Bin Wang, Xiangyang Hao, Yongyan Zhang, Tianchi Wang, Zhenhong Lu, Zhongxiong Lai, Chunzhen Cheng
Summary: Piriformospora indica has been found to promote the growth and enhance the resistance of gerbera plants against root rot disease. It increases the survival rate of tissue cultured seedlings and improves their aboveground and underground fresh weights as well as chlorophyll content. Additionally, P. indica increases the activities of antioxidant enzymes and promotes the accumulation of growth-promoting phytohormones.
SCIENTIA HORTICULTURAE
(2022)
Article
Horticulture
Chengyu Zhang, Shanshan Ni, Shuting Zhang, Munir Nigarish, Chunzhen Cheng, Xu Shen, Fan Liu, Zhengchun Lin, Xiaofang Li, Hao Wu, Qianyu Li, Xiumei Wang, Yuling Lin, Xu XuHan, Zhongxiong Lai
Summary: In this study, we identified 58 MaCYP71s and 14 MbCYP71s from Musa acuminata Colla and Musa balbisiana Colla, and found that MaCYP71s were more conservative than MbCYP71s. Analysis indicated that MaCYP71s may respond to low temperature, and 15 members of the MaCYP71 family were primarily regulated by miRNA and lncRNA. This study suggests that MaCYP71 plays a crucial role in the response of banana to low-temperature stress.
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Ruilian Lai, Xiaopei Wu, Xin Feng, Minxia Gao, Yu Long, Rujian Wu, Chunzhen Cheng, Yiting Chen
Summary: Using lncRNA-seq technology, we identified 591 differentially expressed lncRNAs and 3107 DE genes from kiwifruit stored at 4 degrees C for different durations. These DEGs were predicted to be targets of lncRNAs and were significantly enriched in cell wall modification and pectinesterase activity, which are related to fruit softening during low-temperature storage. Additionally, KEGG analysis showed that these DEGs were associated with starch and sucrose metabolism. Our study highlights the critical regulatory roles of lncRNAs in kiwifruit ripening and softening under low-temperature storage.
Article
Plant Sciences
Bin Wang, Yanbing Xu, Shiyao Xu, Huan Wu, Pengyan Qu, Zheng Tong, Peitao Lu, Chunzhen Cheng
Summary: In this study, 84 SNARE genes were identified from banana and their expression was found to vary in different organs. The expression of many MaSNAREs was responsive to temperature stress and fungal infection. The effects of fungal infection on the expression of some MaSNAREs could be alleviated by prior colonization of beneficial fungus Si, indicating their roles in enhancing banana resistance.
Article
Plant Sciences
Chunzhen Cheng, Rui Yang, Lu Yin, Jianying Zhang, Limin Gao, Rong Lu, Yan Yang, Pengfei Wang, Xiaopeng Mu, Shuai Zhang, Bin Zhang, Jiancheng Zhang
Summary: Carotenoid cleavage oxygenases (CCOs) were identified and characterized in Cerasus humilis. Nine CCO genes were classified into six subfamilies, and showed diverse expression patterns in different organs and ripening stages of fruits. Enzyme assays and gas chromatography/mass spectrometer analysis revealed that ChCCD1 can cleave lycopene and beta-carotene to produce specific apocarotenoids, while ChCCD4 does not have similar functions.
Article
Biochemistry & Molecular Biology
Junqiang Yang, Zhongmei Shen, Pengyan Qu, Rui Yang, Anping Shao, Hao Li, Ailing Zhao, Chunzhen Cheng
Summary: This study reported the therapeutic effect of oxytetracycline hydrochloride (OTC-HCl) on jujube witches' broom disease (JWB) and revealed the impact of JWB infection and treatment on gene expression profiling in jujube.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Zening Zhang, Pengyan Qu, Siyi Hao, Ruide Li, Yongyan Zhang, Qi Zhao, Pengfei Wen, Chunzhen Cheng
Summary: In this study, we characterized and studied the CHS gene family in highbush blueberry. We identified 22 CHS genes and classified them into four subfamilies. Gene expression analysis showed that VcCHSs in subfamily I were highly expressed in late ripening stages of the fruit. By transient overexpression, we found that three VcCHSs could enhance anthocyanin accumulation and up-regulate the expression of flavonoid biosynthetic genes. Promoter analysis revealed multiple cis-acting elements and transcription factor binding sites in VcCHSs. Our study provides insights into the characteristics and functions of blueberry CHSs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Rui Yang, Yan Yang, Yang Hu, Lu Yin, Pengyan Qu, Pengfei Wang, Xiaopeng Mu, Shuai Zhang, Peng Xie, Chunzhen Cheng, Jiancheng Zhang
Summary: Chinese dwarf cherry (Cerasus humilis) is a wild fruit tree and medicinal plant endemic to China. Its fruits are rich in various bioactive compounds, such as flavonoids and carotenoids, which contribute greatly to their high antioxidant capacity. This study compared the contents of bioactive substances and antioxidant capacities in differentially pigmented C. humilis fruits of four varieties. The results revealed that anthocyanin, total flavonoids, and total phenols were the three main components responsible for the antioxidant activity of C. humilis fruits. The study also investigated the expression patterns of genes involved in the biosynthesis of carotenoids and flavonoids, revealing correlations between gene expression and the accumulation of these compounds in the fruits.
Article
Horticulture
Chunzhen Cheng, Jiapeng Liu, Pengyan Qu, Zheng Tong, Yongyan Zhang
Summary: In this study, the MaTDC and MaASMT gene families in the banana genome were investigated, and their expression patterns under low temperature and exogenous melatonin treatments were analyzed. The results showed that the expression of MaTDC4-1, MaTDC8-2, MaTDC10-1, and MaASMT10-1, as well as the endogenous melatonin content in banana leaves, were significantly promoted by low temperature. Furthermore, transient overexpression of MaTDC4-1 and MaASMT4-2 increased melatonin accumulation in tobacco leaf. This study demonstrated the contributions of MaTDC and MaASMT genes, as well as melatonin, to banana cold resistance.
SCIENTIA HORTICULTURAE
(2023)
Article
Biochemistry & Molecular Biology
Yongyan Zhang, Dingquan Huang, Bin Wang, Xuelian Yang, Huan Wu, Pengyan Qu, Li Yan, Tao Li, Chunzhen Cheng, Dongliang Qiu
Summary: In this study, fifteen candidate anthocyanin biosynthesis related MYB (ABRM) proteins were identified from highbush blueberry, including twelve R2R3-MYBs and three 1R-MYBs. The subcellular localization prediction results showed that most of the blueberry ABRMs were nucleus-localized, except for VcRVE8. The gene structure analysis revealed a wide range of exon numbers in the blueberry ABRM genes. The promoters of the blueberry ABRM genes contained various responsive elements related to light, phytohormones, abiotic stress, and plant growth and development. Additionally, the expression analysis showed that VcMYB, VcMYB6, VcMYB23, VcMYBL2, and VcPH4 were abundantly expressed in blueberry fruits, with VcMYB being the highest in red, purple, and blue fruits. The study provides valuable information for future research on anthocyanin biosynthesis related MYBs in blueberry.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Horticulture
Jiapeng Liu, Huan Wu, Bin Wang, Yongyan Zhang, Jiashui Wang, Chunzhen Cheng, Yuji Huang
Summary: Evidence has shown that exogenous melatonin can alleviate cold stress in plants. This study investigated the defensive roles of exogenous melatonin in banana under cold stress. The results demonstrated that exogenous melatonin treatment enhanced the cold resistance of banana by improving antioxidant defense and inducing the expression of cold-responsive genes.
Article
Horticulture
Bin Wang, Yongyan Zhang, Jiapeng Liu, Ou Sheng, Fan Liu, Dongliang Qiu, Peitao Lue, Guiming Deng, Chunzhen Cheng
Summary: A leaf blight disease caused by Alternaria jacinthicola was found on Robusta bananas in Guangdong province, China in September 2020. This disease leads to severe leaf necrosis and economic losses in banana production.