Article
Fisheries
Emmanuelle Moreau, Lionel Pineau, Florine Bachelet, Antoine Rostang, Kenny Oberle, Segolene Calvez
Summary: This study aimed to develop a method of infectious challenge in large Rainbow trout by immersion, simulating natural infection conditions. The results showed that a 24-hour contact period led to infection in all fish with a mortality rate of 53.25%. The infected fish developed acute infection symptoms and lesions similar to furunculosis, and antibodies against the bacterium were produced 4 weeks after challenging compared to the non-challenged group.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mengqun Liu, Xiaodong Yang, Chu Zeng, Hongkui Zhao, Jifang Li, Zhishuai Hou, Haishen Wen
Summary: Rainbow trout is an important commercial fish, but infectious diseases can cause significant economic losses. Our study reveals the pathophysiological responses of the brain and kidney in rainbow trout infected with furunculosis, including neuroinflammation, neural dysfunction, and immunomodulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Fisheries
Jin Woo Jun, Jeong Woo Kang, Sib Sankar Giri, Sang Wha Kim, Sang Guen Kim, Jun Kwon, Sung Bin Lee, Won Joon Jung, Young Min Lee, Su Jin Jo, Se Chang Park
Summary: This study investigated the effect of a starch hydrogel-based oral vaccine on rainbow trout's antibody reactivity, immune-related genes expression, and protection against furunculosis infection. The results showed that the vaccine increased the agglutination titers and improved the survival rate of fish. Furthermore, the expression of immune-related genes was altered after vaccination.
Article
Chemistry, Multidisciplinary
Magdalena Malachowicz, Andrzej K. Siwicki, Stefan Dobosz, Roman Wenne
Summary: In this study, the transcriptional changes in the skin of rainbow trout in response to a bacterial infection were investigated using oligonucleotide microarray technology. The study identified many differentially expressed genes involved in immune response and revealed the connection between metabolic remodeling and immune response.
APPLIED SCIENCES-BASEL
(2023)
Review
Fisheries
Yongji Dang, Yanling Sun, Yangen Zhou, Xianhui Men, Bowen Wang, Bing Li, Yichao Ren
Summary: This study evaluated the effects of probiotics on growth, immune response, and susceptibility to bacterial infection in rainbow trout. The results showed that probiotics promoted weight gain and resistance to bacterial infection in rainbow trout. Different Toll-like receptors were involved in the recognition of beneficial bacteria, and probiotics enhanced the innate immune function of rainbow trout by training immune response pathways.
Article
Immunology
Jeong In Yang, Dagoberto Sepulveda, Irina Vardia, Jakob Skov, Louise Goksoyr, Adam F. F. Sander, Niels Lorenzen
Summary: This study aimed to develop and test a recombinant alternative vaccine based on virus-like particles (VLPs) decorated with VapA protein as the key structural surface protein for combating A. salmonicida bacterial disease. The results showed that rainbow trout vaccinated with VapA-VLP vaccines exhibited comparable protection to the bacterin-based vaccine and produced a strong VapA-specific antibody response.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Hajarooba Gnanagobal, Trung Cao, Ahmed Hossain, Ignacio Vasquez, Setu Chakraborty, Joy Chukwu-Osazuwa, Danny Boyce, Maria Jesus Espinoza, Victor Antonio Garcia-Angulo, Javier Santander
Summary: This study characterized the riboflavin provision pathways of Aeromonas salmonicida and found critical provision genes. The bacterium has multiple forms of riboflavin provision and duplicated provision genes are essential for its infection.
Article
Immunology
Jongwon Lim, Suhee Hong
Summary: This study aimed to identify molecular mechanisms affected by a combined vaccine against Aeromonas salmonicida and Vibrio anguillarum. The vaccine upregulated gene expression associated with adaptive immune response markers and triggered differential expression of hundreds of genes on different days. Gene ontology and Kyoto Encyclopedia of Genetic and Genomic analyses identified immune system-related pathways and processes affected by the vaccine. Protein-protein interaction analysis revealed TNF as the central player in immune-related responses at different time points.
Article
Fisheries
Alexis Viel, Antoine Rostang, Marie-Line Morvan, Catherine Fournel, Patrick Daniel, Chantal Thorin, Sandrine Baron, Pascal Sanders, Segolene Calvez
Summary: The study used a PKPD approach combined with epidemiological data to determine optimal enrofloxacin doses in rainbow trout; results showed enrofloxacin should be considered as a long-acting drug in trout, raising concerns about the standard dosage; fish ploidy had an impact on the PK of enrofloxacin.
Article
Microbiology
Pierre-Etienne Marcoux, Antony T. Vincent, Marie-Ange Massicotte, Valerie E. Paquet, Emilie J. Doucet, Nava Hosseini, Melanie V. Trudel, Gabriel Byatt, Mathilde Laurent, Michel Frenette, Steve J. Charette
Summary: European strains of Aeromonas salmonicida subsp. salmonicida are more prone to losing the TTSS locus compared to Canadian strains. A cluster of genes encoding putative proteins with DNA binding capacity and phage proteins was found to be almost perfectly correlated with the susceptibility of different strains to lose the locus. This discovery opens up new opportunities for studying the thermosensitivity of pAsa5.
Article
Veterinary Sciences
Jiajia Wang, Lijun Xiu, Ying Qiao, Youyu Zhang
Summary: It was previously believed that psychrophilic Aeromonas salmonicida could not infect mammals and humans due to its inability to grow above 25 degrees C. However, a new study found that a specific strain of mesophilic A. salmonicida isolated from Epinephelus coioides was able to cause furunculosis. The researchers discovered that the Zn2+ uptake related genes znuA, znuB, and znuC might play a role in the virulence regulation of A. salmonicida SRW-OG1. Silencing these genes resulted in restricted growth under Fe2+ starvation and decreased motility, biofilm formation, adhesion, and hemolysis. The expression of znuABC was also found to be affected by different growth conditions and stressors.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Microbiology
Baptiste Redivo, Nicolas Derome, Patrick Kestemont, Valerie Cornet
Summary: Environmental stressors can disrupt the microbiota-host relationship and cause loss of function. In this study, the effects of Aeromonas salmonicida infection on the taxonomic composition and structure of the fish microbiota were assessed. The results indicate that the infection can lead to dysbiosis and an increase in opportunistic pathogens, and the findings may contribute to the development of non-invasive disease prevention techniques in aquaculture.
Article
Zoology
Serdar Bektas, Ozer Ayik
Summary: This study experimentally infected rainbow trout with Aeromonas salmonicida and examined the hematological parameters and glucose-6-phosphate dehydrogenase (G6PDH) activity. The results showed that red blood cell count, hemoglobin concentration, and hematocrit significantly decreased after infection. Total leucocyte and thrombocyte counts were significantly higher in the early stage of infection. Additionally, G6PDH activity was significantly increased in the infected group during the first and seventh days.
PAKISTAN JOURNAL OF ZOOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Maisa Garcia-Arguinzonis, Elisa Diaz-Riera, Esther Pena, Rafael Escate, Oriol Juan-Babot, Pedro Mata, Lina Badimon, Teresa Padro
Summary: The study revealed elevated levels of circulating C3 in patients with familial hypercholesterolemia and subclinical coronary atherosclerosis, with enriched C3 products in AT-ECM. However, there was no significant correlation between circulating C3 levels and plaque burden increase, indicating a local regulation of C3 in AT arteries. The research also showed functional effects of C3 active fragments on VSMCs, suggesting a role of the C3 complement system in vascular remodeling and advanced atherosclerotic lesion progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Zoology
Serdar Bektas, Ozer Ayik
Summary: The experimental infection with Aeromonas salmonicida in rainbow trout showed distinct patterns in hematological parameters and glucose-6-phosphate dehydrogenase activity, including changes in red blood cell values, white blood cell and platelet counts, as well as an increase in G6PDH activity.
PAKISTAN JOURNAL OF ZOOLOGY
(2022)
Article
Biochemical Research Methods
Dwight R. Causey, Jin-Hyoung Kim, David A. Stead, Samuel A. M. Martin, Robert H. Devlin, Daniel J. Macqueen
JOURNAL OF PROTEOMICS
(2019)
Article
Immunology
Carola E. Dehler, Katherine Lester, Giulia Della Pelle, Luc Jouneau, Armel Houel, Catherine Collins, Tatiana Dovgan, Radek Machat, Jun Zou, Pierre Boudinot, Samuel A. M. Martin, Bertrand Collet
JOURNAL OF IMMUNOLOGY
(2019)
Article
Fisheries
T. C. Clark, J. Tinsley, D. J. Macqueen, S. A. M. Martin
FISH & SHELLFISH IMMUNOLOGY
(2019)
Article
Multidisciplinary Sciences
Dwight R. Causey, Jin-Hyoung Kim, Robert H. Devlin, Samuel A. M. Martin, Daniel J. Macqueen
SCIENTIFIC REPORTS
(2019)
Article
Food Science & Technology
He Ni, Helen Hayes, David Stead, Guang Liu, Huaijie Yang, Haihang Li, Vassilios Raikos
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
T. C. Clark, J. Tinsley, T. Sigholt, D. J. Macqueen, S. A. M. Martin
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2020)
Article
Microbiology
Anna M. Alessi, Victoria Gray, Freda M. Farquharson, Adriana Flores-Lopez, Sophie Shaw, David Stead, Udo Wegmann, Claire Shearman, Mike Gasson, Elaina S. R. Collie-Duguid, Harry J. Flint, Petra Louis
ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Fisheries
T. C. Clark, J. Tinsley, T. Sigholt, D. J. Macqueen, S. A. M. Martin
FISH & SHELLFISH IMMUNOLOGY
(2020)
Article
Toxicology
Hanna Katarina Lilith Johansson, Terje Svingen, Julie Boberg, Paul A. Fowler, David Stead, Anne Marie Vinggaard, Panagiotis Filis
ARCHIVES OF TOXICOLOGY
(2020)
Article
Immunology
Fiona K. Bakke, Milena M. Monte, David A. Stead, Dwight R. Causey, Alex Douglas, Daniel J. Macqueen, Helen Dooley
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Food Science & Technology
Qianqian Ni, Viren Ranawana, Helen E. Hayes, Nicholas J. Hayward, David Stead, Vassilios Raikos
Article
Immunology
Giuseppe Buda De Cesare, Ahmed Hafez, David Stead, Carlos Llorens, Carol A. Munro
Summary: This study reveals that echinocandin-resistant clinical isolates of Candida albicans exhibit different cell surface characteristics compared to sensitive isolates and show a different response to echinocandin drugs. These findings suggest that drug resistance may involve alterations in cell wall architecture and drug response.
Article
Immunology
Fiona K. Bakke, Manu Kumar Gundappa, Hanover Matz, David A. Stead, Daniel J. Macqueen, Helen Dooley
Summary: This study used mass spectrometry-based proteomics to identify and quantify candidate immune proteins in the plasma of nurse sharks. By integrating transcriptome data, they identified 626 plasma proteins and discovered some proteins involved in the immune response. This research enhances genetic and protein-level resources for nurse shark research and improves our understanding of elasmobranch plasma proteome.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Leandro Jose de Assis, Judith M. Bain, Corin Liddle, Ian Leaves, Christian Hacker, Roberta Peres da Silva, Raif Yuecel, Attila Bebes, David Stead, Delma S. Childers, Arnab Pradhan, Kevin Mackenzie, Katherine Lagree, Daniel E. Larcombe, Qinxi Ma, Gabriela Mol Avelar, Mihai G. Netea, Lars P. Erwig, Aaron P. Mitchell, Gordon D. Brown, Neil A. R. Gow, Alistair J. P. Brown
Summary: This study reveals that beta-1,3-glucan exposure on the surface of Candida albicans cells is targeted during phagocytosis, and lactate-induced masking reduces beta-1,3-glucan exposure. Downstream effectors of protein kinase A (PKA), Sin3 and Mig1/Mig2, regulate the secretion of glucanases and modulate beta-1,3-glucan exposure. Perturbing PKA, Sin3, or Mig1/Mig2 attenuates the virulence of lactate-exposed C. albicans cells.
Article
Cell Biology
Ralfs Buks, Abdo Alnabulsi, Rodanthi Zindrili, Ayham Alnabulsi, Alex Wang, Tiehui Wang, Samuel A. M. Martin, Maria K. Dahle
Summary: The aim of this study is to characterize SAA protein levels in salmonids using a newly developed antibody specific to salmonid SAA. The presence of SAA protein was analyzed in rainbow trout macrophage cell line and rainbow trout challenged with Aeromonas salmonicida- or flagellin-stimulated Atlantic salmon. This study is the first to characterize SAA protein levels in salmonids in vivo and in vitro, and the newly developed salmonid SAA antibody has the potential to be developed into assays for monitoring and evaluating fish health.