4.8 Article

Adaptive evolution of microalgal strains empowered by fulvic acid for enhanced polyunsaturated fatty acid production

期刊

BIORESOURCE TECHNOLOGY
卷 277, 期 -, 页码 204-210

出版社

ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.12.116

关键词

Adaptive evolution; Microalgal strain; Hyposalinity stress; Polyunsaturated fatty acids; Fulvic acid

资金

  1. Natural Science Foundation of China [31870027, 41576132]
  2. Natural Science Foundation of Guangdong [2014A030308010]
  3. China Postdoctoral Science Foundation [2018 M643363]

向作者/读者索取更多资源

Microalgae have emerged as the potential source for value-added products such as polyunsaturated fatty acids (PUFAs). Metabolic engineering of multiple metabolic pathways has promoted eicosapentaenoic acid (EPA) production in microalgae, however, further improvement is warranted owing to the burgeoning demand. Here we improved the microalgal strains by adaptive evolution under hyposalinity treatment, which showed that 70% salinity potentiated the algae to enhance PUFAs. To exploit the maximal PUFA production potential of evolved strains, we subjected evolved algae to light, temperature and fulvic acid treatment. Amongst, fulvic acid (15 mg/L) enhanced growth and achieved the highest EPA content (13.9%) in the evolved diatom. Fulvic acid enhanced antioxidant potential and unprecedently governed the expression of PUFA and lipid biosynthetic genes. Collectively, this investigation demonstrates the efficacy of adaptive evolution empowered by fulvic acid and exemplifies a feasible strain improving strategy to harness the biotechnological potential of microalgae.

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