Article
Forestry
Maria E. Eugenio, Gabriela Dominguez, Jose M. Molina-Guijarro, Manuel Hernandez, Maria E. Arias, David Ibarra
Summary: This study evaluates the potential of laccase and mannanase enzymes from Streptomyces ipomoeae for improving the conventional hydrolysis of steam-pretreated softwood. It was found that laccase treatment and mannanase supplementation can significantly increase glucose and xylose production while reducing lignin content.
WOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Green & Sustainable Science & Technology
Zhi-Yuan Bai, Shuai You, Fang Zhang, Zhi-Wei Dong, Yi-Fan Zhao, Hong-Jian Wen, Jun Wang
Summary: The study modified xylanase through rational design and used it with cellulase to synergistically degrade mulberry branches pretreated with seawater. The mutant S21Y/N318W showed improved thermostability and specific activity. Under the condition of seawater immersion pre-treatment, the highest yield of fermentable sugar and degree of synergy were achieved. This study provides an efficient method of biomass saccharification for biofuel production.
Article
Biotechnology & Applied Microbiology
Sneeha Veerakumar, Rameshpathy Manian
Summary: The coproduction of amylase, agarase, and xylanase by the bacterium Halomonas meridiana VITSVRP14 isolated from seaweed was achieved using submerged fermentation. Optimization of pH, temperature, and lactose concentration resulted in a significant increase in enzyme activity. The coproduced enzyme mixture showed high saccharification rates on various lignocellulosic substrates, indicating its potential in biomass saccharification.
FERMENTATION-BASEL
(2022)
Article
Polymer Science
Dae-Seok Lee, Younho Song, Yoon-Gyo Lee, Hyeun-Jong Bae
Summary: This study investigated the adsorption of cellulase onto different types of lignin and found that the hydrophobic properties of carbohydrate binding motif (CBM) and enzyme structure are key factors in the adsorption, while the properties of specific lignin types indirectly affect the adsorption.
Article
Biotechnology & Applied Microbiology
Hadjer Djelid, Sigrid Flahaut, Yamina Oudjama, Corinne Vander Wauven, Noreddine Kacem Chaouche
Summary: This study reports the purification and characterization of an extremely halotolerant ss-mannanase ManH1 from B. velezensis strain H1. The enzyme showed good thermostability and salt activation, with increased activity in the presence of NaCl. It also demonstrated the ability to produce oligosaccharides from lignocellulosic biomass, indicating its potential commercial value.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Materials Science, Paper & Wood
Elisa Sponla, Jenni Rahikainen, Antje Potthast, Stina Gronqvist
Summary: The sustainability of regenerated cellulosic fibres can be improved by using paper grade pulp instead of dissolving pulp, but adjusting the molar mass distribution (MMD) is necessary for good solubility and spinnability. Enzymatic treatments were used to adjust the MMD of softwood and eucalyptus kraft pulps, and it was found that eucalyptus pulp required a higher dose of endoglucanase compared to softwood pulp. Combining xylanase with endoglucanase reduced the required endoglucanase dose for eucalyptus pulp, indicating that xylan inhibited the enzymatic treatment. The different processabilities of the pulps were attributed to differences in cellulose accessibility and hemicellulose composition and localization.
Review
Food Science & Technology
Monika Rana, Sunena Jassal, Richa Yadav, Anupama Sharma, Neena Puri, Koushik Mazumder, Naveen Gupta
Summary: One of the emerging non-digestible oligosaccharide prebiotics is beta-mannooligosaccharides (beta-MOS), which can selectively promote the growth of beneficial microorganisms while inhibiting enteric pathogens. Production of beta-MOS using enzymes is the most effective and eco-friendly approach. Thorough in-vivo and clinical studies are needed for the application of beta-MOS.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Food Science & Technology
Hilal Nur Gurler, Cansu Yilmazer, Selime Benemir Erkan, Ali Ozcan, Ercan Yatmaz, Hatice Reyhan Oziyci, Mustafa Karhan, Irfan Turhan
Summary: The use of mannanases in enzymatic maceration of carrot juice can increase extract yield of soluble compounds like sugars and beta-carotene. By optimizing enzyme concentrations and combinations, improvements in yield and quality of carrot juice, particularly in beta-carotene content, can be achieved. Enzyme liquefaction process with mannanases, either individually or in combination with industrial enzymes, proves to be a crucial step in enhancing carrot juice production.
JOURNAL OF FOOD PROCESSING AND PRESERVATION
(2021)
Article
Biotechnology & Applied Microbiology
Jinkang Chen, Hao Qin, Chaoqun You, Lingfeng Long
Summary: This study cloned and expressed thermostable xylanase and beta-xylosidase genes from Pseudothermotoga thermarum, and demonstrated their stability at high temperature and synergistic hydrolysis of cellulose, suggesting their potential application in the saccharification process.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Food Science & Technology
Naveen Kango, Uttam K. Jana, Ritumbhara Choukade, Suresh Nath
Summary: This article presents the latest developments related to the production and applications of prebiotic mannooligosaccharides (MOS), highlighting the health benefits they provide, including antineoplastic, immunomodulatory, and hypolipidemic effects. The article also explores the potential usage of MOS as functional food ingredients for humans.
CURRENT OPINION IN FOOD SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Eunjoo Kim, Amy F. Moss, Natalie K. Morgan, Kosar Gharib-Naseri, Peter Ader, Mingan Choct
Summary: The present study investigated the effect of supplementing fibre-degrading enzymes on the severity of subclinical necrotic enteritis (NE) in broiler chickens offered wheat- or maize-based diets. The findings showed that adding fibre-degrading enzymes can alleviate the negative impact of NE on growth performance in birds, and the type of diet and enzyme supplementation affect growth performance and digesta viscosity in the birds.
Article
Environmental Sciences
Steffy Angural, Sunena Jassal, Rahul Warmoota, Monika Rana, Neena Puri, Naveen Gupta
Summary: One of the major focuses in the paper industry is shifting towards eco-friendly paper production by replacing chemical-based bleaching with enzymatic biobleaching. The application of a cocktail of enzymes has been explored to overcome the limitations of using a single enzyme for pulp biobleaching. This short communication summarizes and discusses various studies in this regard, providing useful information for further research and greening the papermaking process.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Fengzhen Zheng, Abdul Basit, Zhiyue Zhang, Huan Zhuang, Jun Chen, Jianfen Zhang
Summary: In this study, the expression of mannanase from Trichoderma asperellum ND-1 was enhanced by using propeptide in Pichia pastoris. The modified enzyme showed increased activity and retained stability in the presence of ethanol and NaCl. The mannanase also exhibited transglycosylation action and could yield fermentable sugars from lignocellulosic biomass, providing valuable insights for high-value biomolecule and biofuel production.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Applied
Rahul Kumar Suryawanshi, Naveen Kango
Summary: Aspergillus quadrilineatus endo-beta-mannanase can effectively degrade various polysaccharides, with hydrolysates from different sources exhibiting varying degrees of cytotoxicity on cells. Lactobacilli produce different types and amounts of short chain fatty acids and branched chain amino acids when fermenting MOS, depending on the source of the hydrolysate.
Article
Biotechnology & Applied Microbiology
Shikha Devi, Divya Dwivedi, Arvind Kumar Bhatt
Summary: The study investigates the utilization of various agro-industrial wastes for cost-effective production of xylanase by Bacillus safensis XPS7. The combination of wheat straw and wheat bran is found to be a cheap and abundant carbon source for hyper production of xylanase. This waste utilization approach helps reduce environmental problems associated with waste disposal.
FERMENTATION-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Yiran Qu, Innocent Bekard, Ben Hunt, Jamie Black, Louis Fabri, Sally L. Gras, Sandra. E. Kentish
Summary: This study compares the performance of a nanofiber device and a resin column for antibody capture. The nanofiber device has a larger housing volume and lower binding capacity, but comparable eluate purity to the resin column. It shows high stability, can be used for multiple cycles, and maintains consistent eluate quality when scaled up. The use of a single nanofiber device can significantly reduce costs compared to a resin column, especially when the number of batches is limited.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Brandon Moore, Christos Georgakis, Chris Antoniou, Sarwat Khattak
Summary: Fed-batch cell culture processes are commonly used in biomanufacturing due to their simplicity and applicability in cGMP environments. However, the challenge lies in the changing physiochemical conditions within the bioreactor as the cell density changes. Traditional response surface models (RSMs) are commonly used for optimization but are limited by their use of time-invariant factors. Dynamic RSM (DRSM) models can predict the time-dependent impact of process inputs, allowing for optimization of process operations that change over time.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Lin Li, Yunfan Bai, Chuhua Qi, Yile Du, Xiaoxiao Ma, Yutong Li, Pingping Wu, Shuangli Chen, Sijing Zhang
Summary: A succinic anhydride-modified apple pomace (SAMAP) was synthesized to address environmental issues caused by the accumulation of apple pomace and effectively treat heavy metal ions. SAMAP exhibited high adsorption capacity for Cu(II) and Pb(II), suggesting its potential application in wastewater treatment.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Peter Satzer
Summary: Water for injection (WFI) production in the biopharmaceutical industry consumes excessive amounts of water and energy. Recycling buffers can potentially save up to 90% of resources, but achieving the full theoretical potential is impossible when a risk-aware design is used. Universal risk-based assessment is important for regulatory authorities to consider the implementation of such a strategy.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Gaoya Sun, Lingkai Jin, Guangxue Wang, Xiaoge Wang, Jin Huang
Summary: In this study, heterologous expression and homologous overexpression of ABC transporter proteins AatA and MdlB were found to improve butyric acid production in C. tyrobutyricum. The overexpression of these proteins upregulated the expression levels of key enzymes in the acetate synthesis pathway and promoted the synthesis and secretion of acetic acid. Additionally, the increase in ATPase activity facilitated sugar utilization, induced extracellular secretion of acetate, and shortened fermentation periods.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Taiki Okamura, Rina Aritomi, Takuya Matsumoto, Ryosuke Yamada, Hidehiko Hirakawa, Hiroyasu Ogino
Summary: In this study, proline was introduced to improve the stability of putidaredoxin reductase (PdR) in the Pseudomonas putida cytochrome P450 system. It was found that PdR_T221P had a longer half-life at high temperatures compared to wild-type PdR, but a shorter half-life in the presence of methanol. Molecular dynamics simulations supported these findings.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yuying Hu, Xiaofan Wang, Shihao Zhang, Zimu Liu, Tengfang Hu, Xin Wang, Xiaoming Peng, Hongling Dai, Jing Wu, Fengping Hu
Summary: This study investigates the effect of iron-carbon micro-electrolysis (ICME) materials on high-solid anaerobic digestion (HSAD). The results show that ICME materials promote methane production in HSAD by increasing the attachment area of microorganisms and facilitating symbiotic metabolism of certain bacterial species. This study provides new insights into microbial mechanisms and enhances our understanding of ICME material enhancement in HSAD.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Jibao Liu, Yufeng Xu, Yuansong Wei
Summary: This study investigated the role of sludge rheology in anaerobic digestion (AD) and found that rheological properties increased with the increase of solid content, resulting in a negative effect on methane production. An extended ADM1 model revealed that enhanced sludge rheological properties increased mass diffusion resistance and reduced uptake rate of acetate.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Xiaoyan Liu, Zongze Chen, Dewen Kong, Xinying Zhang, Chuanhua Wang, Yongqi Wang
Summary: This study explored the role of intracellular and extracellular enzymes of Acinetobacter baumannii and Talaromyces sp. in the degradation of crude oil. The extracellular enzymes of Talaromyces sp. were more effective in degrading n-alkanes, while those of Acinetobacter baumannii had a better effect on aromatic hydrocarbons. The degradation enzyme systems of both bacteria and fungi complemented each other, improving the overall degradation ability.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Jing Dong, Lingli Xu, Yuxiang Liu, Li Ren, Ke Yuan
Summary: The utilization of biochar-immobilized microorganisms is an effective method for eliminating phenol from water. The high susceptibility of bacteria to environmental factors is a challenge for practical implementation. In this study, biochar was used to reduce microbial susceptibility and enhance phenol removal. The addition of biochar altered the dominant species of phenol-degrading bacteria and response surface analysis indicated the significant influence of biochar pyrolysis temperature and experimental temperature on phenol removal rate.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Zibin Pan, Mengying Liu, Zuliang Chen
Summary: This study successfully removed metalloids and heavy metals from acid mine drainage (AMD) using bio-synthesized Fe/Cu nanoparticles (Fe/Cu NPs). The Fe/Cu NPs showed high removal capacities and the presence of organic substances contributed to their stability.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Guangbing Liu, Han Zhang, Jincan Huang, Lu Zhang, Teng Zhang, Xuemin Yu, Weijing Liu, Chunkai Huang
Summary: This study investigated the effect of Fenton pre-treatment on the treatment efficiency of printing and dyeing wastewater (PDW) using two anaerobic/aerobic-membrane bioreactors (A/O-MBRs). The results showed that Fenton pre-treatment significantly improved the removal efficiency of COD and AOX in PDW, and reduced membrane fouling. The Shannon indices and metagenomics analysis indicated that the microbial diversity in anaerobic flocs was higher than that in aerobic flocs, and EC3.1.1.45 and pcaI were identified as key functional genes.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Lidia Borgosz, Duygu Dikicioglu
Summary: The Industrial Internet of Things (IIoT) is a system that connects devices and provides real-time insight into industrial processes. However, the complexity and regulatory requirements of the biomanufacturing sector make it challenging to implement IIoT. There is a need for universal solutions to overcome this challenge and advance the field of biomanufacturing.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yajie Li, Weikang Kong, Yuyao Zhang, Huarui Zhou, Hongbo Liu, Salma Tabassum
Summary: In this study, the iron-carbon multi-micro electric field coupling anaerobic co-digestion technique was used to treat coal gasification wastewater (CGW). The experimental results showed that under optimal operating conditions, this technique can significantly reduce the toxicity of the wastewater and achieve high removal efficiencies. Additionally, the analysis of microbial communities revealed that the coupling system promotes direct interspecies electron transfer.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Aparecido Nivaldo Modenes, Debora Gozzi Fernandes, Daniela Estelita Goes Trigueros, Matheus Guilherme Amador, Fernando Rodolfo Espinoza-Quinones, Taysa de Souza Braniz, Adilson Ricken Schuelter, Glacy Jaqueline da Silva, Lucimar Pereira Bonett
Summary: This study aimed to systematically remove organic pollutants from raw dairy wastewater with high concentrations of COD, TOC, and TN using Poterioochromonas malhamensis algae strains. The results showed that the biomass yield rate using FP-PBRs was 10% higher than tubular PBRs, and the organic pollution in wastewater was significantly reduced with a decrease of about 98% in COD, 95% in TN, and 92% in TOC.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)