Article
Immunology
Amanda Brosius Lutz, Tawaun A. Lucas, Glenn A. Carson, Christine Caneda, Lu Zhou, Ben A. Barres, Marion S. Buckwalter, Steven A. Sloan
Summary: The response of Schwann cells to peripheral nerve injury involves the modulation of specific cellular pathways, including the downregulation of the AGE/RAGE pathway and secreted molecules Sparcl1 and Sema5a. These findings contribute to our understanding of neuroinflammation and provide valuable insights for improving CNS regeneration.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Biochemistry & Molecular Biology
Luca Scaccini, Roberta Mezzena, Alessia De Masi, Mariacristina Gagliardi, Giovanna Gambarotta, Marco Cecchini, Ilaria Tonazzini
Summary: The study presents a method using chitosan scaffolds to promote nerve regeneration, showing that asymmetric directional topographies can enhance cell proliferation and migration speed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Surgery
Akira Ikumi, Anne Gingery, Yoichi Toyoshima, Chunfeng Zhao, Steven L. Moran, Christopher Livia, Tyler Rolland, Timothy Peterson, Michael S. Sabbah, Soulmaz Boroumand, Tiam M. Saffari, Atta Behfar, Alexander Y. Shin, Peter C. Amadio
Summary: The study demonstrated improved functional motor recovery and nerve regeneration profiles in a rat sciatic nerve reverse autograft model with the local administration of purified exosome product. This suggests beneficial effects on nerve regeneration, gene profiles, and motor outcomes.
PLASTIC AND RECONSTRUCTIVE SURGERY
(2021)
Review
Biochemistry & Molecular Biology
Liam A. McMorrow, Adrian Kosalko, Daniel Robinson, Alberto Saiani, Adam J. Reid
Summary: Patients with major peripheral nerve injury have poor recovery outcomes due to slow axonal regeneration and deterioration in the ability of the distal stump to support axonal growth. Schwann cells play a crucial role in nerve regeneration, but chronic denervation leads to instability in their repair phenotype and hinders regeneration support. Recent research on the cellular machinery, particularly the role of c-Jun, has opened opportunities for therapeutic intervention to support prolonged neural regeneration.
Article
Engineering, Biomedical
Xianhao Dong, Siyang Liu, Yueyue Yang, Shan Gao, Wenlei Li, Jiasong Cao, Ye Wan, Ziqi Huang, Guanwei Fan, Quan Chen, Hongjun Wang, Meifeng Zhu, Deling Kong
Summary: Research on nerve conduits composed of oriented microfiber-bundle cores and randomly organized nanofiber sheaths has shed light on the regulatory role of microfiber orientation in promoting peripheral nerve regeneration, elucidating a cascade of biological responses and ultimately enhancing nerve regeneration outcomes.
Article
Neurosciences
Estefania Contreras, Sara Bolivar, Xavier Navarro, Esther Udina
Summary: Neurons in the peripheral nervous system have the ability to regenerate their axons after injury by triggering a complex activation response. Schwann cells (SCs) play an active role in regulating axonal programs and orchestrating Wallerian degeneration and axonal regeneration through both cell-to-cell contacts and secreted signals. The secretome, which includes proteins, cytokines, growth factors, and extracellular vesicles, facilitates communication and is essential for axonal regeneration. The use of secretome has emerged as a promising alternative to cell therapy, reducing the risks associated with cell use. In this review, the roles of SC and macrophage secretomes in peripheral nerve injury are discussed, along with the use of secretomes in experimental models to enhance nerve regeneration.
EXPERIMENTAL NEUROLOGY
(2022)
Article
Immunology
Eri Oshima, Yoshinori Hayashi, Zhen Xie, Hitoshi Sato, Suzuro Hitomi, Ikuko Shibuta, Kentaro Urata, Junjun Ni, Koichi Iwata, Tatsuo Shirota, Masamichi Shinoda
Summary: This study aimed to identify the axon regeneration factor derived from M2 macrophages. Transcriptome analysis revealed the upregulation of the macrophage-selective lysosomal protease CTSS in the injured nerve. Macrophage ablation experiments in a rat model showed that ablation at the injured site hindered partial recovery from sensory deficits. CTSS was upregulated in the injured nerve and facilitated endogenous recovery from hypoesthesia. Additionally, CTSS promoted axon regeneration by activating Schwann cells through Ephrin-B2 shedding from fibroblasts.
JOURNAL OF NEUROINFLAMMATION
(2023)
Article
Neurosciences
Jun Sun, Zhi Liao, Zhangyu Li, Hao Li, Zhimin Wu, Chuan Chen, Hui Wang
Summary: This study focused on investigating the roles and mechanisms of exosomes derived from Schwann cells under different conditions in regulating macrophagic sub-phenotypes and peripheral nerve injury repair. The study found that exosomes from normal oxygen condition Schwann cells promoted M2 macrophagic polarization and facilitated axon elongation, while exosomes from post-injury oxygen-glucose-deprivation-condition Schwann cells promoted M1 polarization and inhibited axon elongation. The down-regulation of miR-146a-5p was closely related to the shift of exosomes to pro-inflammatory phenotype. The study concluded that miR-146a-5p played an important role in regulating macrophagic phenotype and had implications for axonal regeneration and functional recovery.
EXPERIMENTAL NEUROLOGY
(2023)
Article
Neurosciences
Deepak Prasad Gupta, Anup Bhusal, Md Habibur Rahman, Jae-Hong Kim, Youngshik Choe, Jaemyung Jang, Hyun Jin Jung, Un-Kyung Kim, Jin-Sung Park, Lee-so Maeng, Kyoungho Suk, Gyun Jee Song
Summary: This study reveals the essential role of EBP50 in the repair function of Schwann cells and regeneration following nerve injury. EBP50 deficiency leads to deficits in Schwann cell functions and delays nerve repair and functional recovery. Additionally, EBP50 enhances regeneration by binding and activating ErbB2.
PROGRESS IN NEUROBIOLOGY
(2023)
Article
Cell Biology
Meng-Ju Lin, Chia-Ming Lee, Wei-Lin Hsu, Bi-Chang Chen, Shyh-Jye Lee
Summary: Macrophages may physically alleviate nerve inhibition and promote regeneration and development in the zebrafish lateral line.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Jue Ling, Chang He, Shuxuan Zhang, Yahong Zhao, Meifeng Zhu, Xiaoxuan Tang, Qiaoyuan Li, Liming Xu, Yumin Yang
Summary: This article provides an overview of evaluation methods used to study scaffold-based therapies for peripheral nerve injury (PNI) in experimental animal models, with particular focus on Schwann cell functions and axonal growth within the regenerated nerve.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Neurosciences
Yunsong Zhang, Yinying Shen, Li Zhao, Qian Zhao, Lili Zhao, Sheng Yi
Summary: Nerve injury-induced Schwann cell dedifferentiation promotes axon growth by creating a favorable microenvironment. Transcription factor BCL11A plays a crucial role in Schwann cell reprogramming during peripheral nerve regeneration. Silencing Bcl11a reduces Schwann cell viability, proliferation, migration rates, and debris clearance ability. Bcl11a deficiency leads to restricted axon elongation and myelin wrapping, resulting in failed recovery. Mechanistically, BCL11A regulates Schwann cell activity by binding to the Nr2f2 promoter and modulating Nr2f2 expression. In conclusion, BCL11A is essential for Schwann cell activation and peripheral nerve regeneration, making it a potential therapeutic target for peripheral nerve injury treatment.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yaxian Wang, Fuchao Zhang, Yunsong Zhang, Qi Shan, Wei Liu, Fengyuan Zhang, Feiyu Zhang, Sheng Yi
Summary: Research findings indicate that Btc plays essential roles in regulating Schwann cell migration and axon elongation, suggesting the potential application of Btc as a regenerative strategy for treating peripheral nerve injury.
MOLECULAR MEDICINE
(2021)
Review
Neurosciences
Jenica Acheta, Shannon B. Z. Stephens, Sophie Belin, Yannick Poitelon
Summary: This review discusses the known and possible molecular mechanisms activated in repair Schwann cells in response to low-intensity ultrasound (LIU), drawing support and attention to LIU as a compelling regenerative treatment for peripheral nerve injury.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Polymer Science
Yuye Huang, Kailei Xu, Jingyi Liu, Guangli Dai, Jun Yin, Peng Wei
Summary: This study investigated the co-culture of Schwann cells (SCs) and rat adrenal pheochromocytoma (PC-12) cells on a 50% degree of methacryloyl substitution gelatin methacrylate (GelMA) scaffold. PC-12 cells showed enhanced cell proliferation and axonal extension in the presence of neurotrophic factors secreted by SCs. A 5% GelMA scaffold was found to better support SCs proliferation, viability, and myelination compared to a 10% GelMA scaffold.
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)