Article
Biotechnology & Applied Microbiology
Sara Saffari, Daan J. Rademakers, Nicholas Pulos, Alexander Y. Shin
Summary: Modulating the nerve's microenvironment using exosomes can enhance nerve regeneration. This study aimed to determine the optimal dose of purified exosome product (PEP) for maximal neurite extension. Treatment with 5% PEP resulted in the longest neurite extension and was superior to the untreated control, while treatment with higher concentrations showed no significant difference. Live cell imaging suggested that PEP migrated towards the neural regeneration site. Overall, 5% PEP was found to be the optimal concentration for nerve regeneration based on this in vitro dose-response analysis.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Cell Biology
Ruslan Masgutov, Alina Zeinalova, Alexey Bogov, Galina Masgutova, Ilnur Salafutdinov, Ekaterina Garanina, Valeriia Syromiatnikova, Kamilla Idrisova, Adelya Mullakhmetova, Dina Andreeva, Liliya Mukhametova, Adilet Kadyrov, Igor Pankov, Albert Rizvanov
Summary: The plasmid construct pBud-coVEGF165-coFGF2 possesses no immunogenicity and biosafety when injected into the intact rat sciatic nerve, showing significant changes in pro-angiogenic stimuli and levels of cytokines in peripheral blood, suggesting systemic effects. Transient expression of growth factors and active transport of transcripts in the sciatic nerve indicate its potential for nerve regeneration when used as a gene therapy drug.
NEURAL REGENERATION RESEARCH
(2021)
Article
Clinical Neurology
Alper Geyik, Basar Koc, Serap Cilaker Micili, Muge Kiray, Haluk Vayvada, Selin Guler
Summary: Decorin protein reduces fibrosis and enhances motor and sensory recovery both clinically and histologically, showing potential as a treatment option for peripheral nerve injuries.
NEUROLOGICAL RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Qijun Li, Shiqiang Gong, Weifan Yao, Ziting Yang, Renjun Wang, Zhaojin Yu, Minjie Wei
Summary: This study utilized a dual-sensitive hydrogel loaded with exosomes derived from hUCMSCs to promote wound healing and skin regeneration, showing significant improvements in wound closure, re-epithelialization rates, and collagen deposition in the wound sites. The results suggest the potential for achieving complete skin regeneration through the use of this novel approach.
Article
Neurosciences
Xiangyu Zeng, Wei Bian, Ziwen Liu, Jianming Li, Shuai Ren, Jian Zhang, Haoran Zhang, Bu Tegeleqi, Guanyi He, Mingyan Guan, Zewei Gao, Chi Huang, Jianyu Liu
Summary: This study demonstrated that muscle-derived stem cell (MDSC) exosomes with overexpressed miR-214 can promote the proliferation and migration of Schwann cells (SCs), increase the expression of neurotrophic factors, and positively affect the recovery of nerve structure and function.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2023)
Article
Oncology
Un Chul Park, Susanna S. Park, Bo Hee Kim, Sung Wook Park, Young Joo Kim, Whitney Cary, Johnathon D. Anderson, Jan A. Nolta, Hyeong Gon Yu
Summary: The study evaluated the protective effects of subretinal or intravitreal injection of human CD34+ BMSC on retinal degeneration and found that injection of CD34+ BMSC could partially rescue degeneration and result in regional preservation of the outer retina near the injection site.
ANNALS OF TRANSLATIONAL MEDICINE
(2021)
Article
Cell Biology
Yu-Song Yuan, Fei Yu, Ya-Jun Zhang, Su-Ping Niu, Hai-Lin Xu, Yu-Hui Kou
Summary: This study investigated the expression levels of various factors in the sciatic nerve after injury, providing insights into the pathophysiology of peripheral nerve repair. The results revealed different trajectories of factors expression, which could potentially aid in the development of clinical treatments for peripheral nerve injury.
NEURAL REGENERATION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Raphaelle Corremans, Patrick C. D'Haese, Benjamin A. Vervaet, Anja Verhulst
Summary: The study successfully developed a rat model of DKD by combining STZ/NAD and oral L-NAME, inducing diabetes, hypertension, and characteristic features of DKD, providing a new animal model for DKD research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Orthopedics
Emeline Maurice, Thibault Godineau, Diane Pichard, Hanane El Hafci, Gwennhael Autret, Morad Bensidhoum, Veronique Migonney, Mathieu Manassero, Veronique Viateau
Summary: This study presents a cost-effective, new relevant and objective rat model associated with low morbidity for the preliminary study of bio-implantable materials designed for remnants-preserving ACL surgery after mid-substance ACL tear.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Dawid Szczepankiewicz, Piotr Celichowski, Pawel A. Kolodziejski, Ewa Pruszynska-Oszmalek, Maciej Sassek, Przemyslaw Zakowicz, Ewa Banach, Wojciech Langwinski, Kosma Sakrajda, Joanna Nowakowska, Magdalena Socha, Ewelina Bukowska-Olech, Joanna Pawlak, Joanna Twarowska-Hauser, Leszek Nogowski, Janusz K. Rybakowski, Aleksandra Szczepankiewicz
Summary: The study found that chronic lithium treatment significantly influenced gene expression profile in both mania and depression models, particularly in the genes enriched in olfactory and taste transduction pathway and long non-coding RNAs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Clinical Neurology
Karlijn L. Kooij, Mieneke C. M. Luijendijk, Lisa Drost, Gerard Platenburg, Annemarie van Elburg, Roger A. H. Adan
Summary: The atypical antipsychotic drug olanzapine has beneficial effects in the activity-based anorexia (ABA) model, mainly by affecting running wheel activity. Intranasal administration of olanzapine rapidly reduces locomotor activity and increases body weight. Further investigation of intranasal olanzapine administration in anorectic patients is recommended.
EUROPEAN NEUROPSYCHOPHARMACOLOGY
(2023)
Article
Neurosciences
Suyash Singh, Arun Kumar Srivastava, Atul K. Baranwal, Ankur Bhatnagar, Kuntal Kanti Das, Sushila Jaiswal, Sanjay Behari
Summary: The study evaluated the efficacy of a silicone conduit in repairing injured nerves using a rat sciatic nerve injury model. Results showed some clinical improvement with the silicone conduit, but overall recovery was suboptimal. The silicone conduit may be a cost-effective alternative for short nerve segments.
Article
Orthopedics
Keijiro Kanno, Miyako Narita, Yuya Kawarai, Shigeo Hagiwara, Satoshi Yoh, Junichi Nakamura, Sumihisa Orita, Kazuhide Inage, Takane Suzuki, Seiji Ohtori
Summary: Tramadol administration alleviated mechanical hyperalgesia induced by MIA injection into the rat hip joint, and significantly suppressed CGRP expression in DRG. However, the progression of OA changes was significantly exacerbated in the MIA + Tramadol group compared to the MIA + Vehicle group.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2022)
Article
Immunology
Tiffany Kirkaldy Spaanager Sztuk, Neil Marcus Rigby, Lasse Norskov-Nielsen, Stef J. Koppelman, Ana Isabel Sancho, Niels-Peter Hell Knudsen, Justin Marsh, Philip Johnson, Shashank Gupta, Alan Robert Mackie, Jeppe Madura Larsen, Katrine Lindholm Bogh
Summary: This study compared the preventive effect of allergen-specific immunotherapy through different routes of peanut allergen delivery in rats. Results showed that high dose oral and sublingual administration, as well as high and medium dose intragastric administration, prevented peanut allergy. Therefore, the route of allergen delivery plays a significant role in preventing peanut allergy.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell & Tissue Engineering
Jincheng Zhang, Hengan Ge, Jun Li, Liyang Chen, Jiaqi Wang, Biao Cheng, Zhitao Rao
Summary: Controlled release and administration of multifunctional therapeutic agents is an effective strategy for treating nerve injury. In this study, a dual-delivery three-dimensional nanofibrous matrix loaded with melatonin and nerve growth factor was developed for peripheral nerve regeneration. The matrix promoted adipose-derived stem cell differentiation and improved sciatic nerve regeneration in rats, demonstrating its potential for peripheral nerve regeneration.
REGENERATIVE THERAPY
(2023)
Article
Endocrinology & Metabolism
Shun Okuwaki, Toru Funayama, Akira Ikumi, Yosuke Shibao, Kousei Miura, Hiroshi Noguchi, Hiroshi Takahashi, Masao Koda, Masaki Tatsumura, Haruo Kawamura, Masashi Yamazaki
Summary: This study aimed to investigate the risk factors for vertebral deformity 6 months after osteoporotic vertebral fractures (OVFs) at the time of injury. The results showed that factors such as 25(OH)D levels and vertebral instability (VI) were significant risk factors for vertebral collapse rate (CR) and kyphotic angle (KA) at 6 months post-injury. Additionally, the CSA of the psoas major and VI were found to significantly affect the KA at the 6-month follow-up.
JOURNAL OF BONE AND MINERAL METABOLISM
(2022)
Article
Surgery
Meiwand Bedar, Tiam M. Saffari, Aaron J. Johnson, Alexander Y. Shin
Summary: In a rat model, acellular nerve allografts (ANA) treated with stem cell delivery and surgical angiogenesis demonstrated early favorable immune responses and improved vascularity at longer follow-up.
JOURNAL OF PLASTIC RECONSTRUCTIVE AND AESTHETIC SURGERY
(2022)
Review
Physiology
Andrew L. O'Brien, Julie M. West, Tiam M. Saffari, Minh Nguyen, Amy M. Moore
Summary: Peripheral nerve injuries can result in life-altering functional deficits, even with the ability of peripheral nerves to regenerate axons after injury. Advances in nerve regeneration and repair, such as electrical stimulation, gene therapy, and surgical technique advancements, are crucial for overcoming the physiological shortcomings of axonal regeneration.
Article
Medicine, General & Internal
Kazuhiro Ikeda, Takeshi Ogawa, Akira Ikumi, Yuichi Yoshii, Sho Kohyama, Reimi Ikeda, Masashi Yamazaki
Summary: The study aimed to investigate the diagnostic accuracy of magnetic resonance imaging for lateral epicondylitis severity, with individual evaluations showing significant improvement in diagnostic accuracy for disease severity.
Article
Orthopedics
Tiam M. Saffari, Maria T. Huayllani, Amy M. Moore
Summary: Diverse leadership is present in Hand Surgery, and we understand the challenges as well as the different perspectives of those who have succeeded in breaking barriers. We are making strong efforts to diversify Hand Surgery because diversity brings excellence by incorporating a wide range of thoughts, backgrounds, and perspectives. We have confidence that these efforts will lead to an increase in the number of diverse leaders and a redefinition of success.
Review
Biochemistry & Molecular Biology
Luke Juckett, Tiam Mana Saffari, Benjamin Ormseth, Jenna-Lynn Senger, Amy M. M. Moore
Summary: Peripheral nerve injuries (PNI) often result in lifelong disability with rare complete functional recovery. Intraoperative electrical stimulation (ES) is a promising surgical adjunct that can augment the intrinsic molecular pathways of nerve regeneration. Clinical trials have shown that ES can be an efficacious therapy to improve patient outcomes following PNIs.
Article
Medicine, General & Internal
Akira Ikumi, Yuichi Yoshii, Yusuke Eda, Tomoo Ishii
Summary: The present study aimed to define the three-dimensional locations and standard measurements of the distal radius of a normal wrist joint. CT images of normal wrists were reconstructed, and several reference points and measurements were evaluated. Significant differences were found between males and females. These measurements can be used to identify the dislocation direction and reduction conditions of distal radius fractures in 3D images.
Article
Orthopedics
Tiam M. Saffari, Amy M. Moore, Ryan W. Schmucker
Article
Medicine, General & Internal
Akira Ikumi, Yuichi Yoshii, Takamasa Kudo, Sho Kohyama, Takeshi Ogawa, Yuki Hara, Tomoo Ishii
Summary: This study investigated the relationship between median nerve cross-sectional area (CSA) and physical characteristics in patients with unilateral symptomatic carpal tunnel syndrome (CTS). The results revealed that the median nerve CSA was larger on the symptomatic side compared to the asymptomatic side. Median nerve CSA correlated with body weight and BMI on the asymptomatic side, but not on the symptomatic side. No correlations were found between median nerve CSA and disease duration or the results of electrodiagnostic testing (EDX) in both sides.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Education, Scientific Disciplines
Bhagat Manku, Tiam Mana Saffari, Vinesh Sandhu, Ankur Khajuria
Summary: This study aimed to assess students' and junior doctors' attitudes on their current knowledge, confidence, and preparedness of appraising and conducting systematic reviews and meta-analysis internationally. The results showed that the majority of participants were from lower middle-income countries and were unfamiliar with the PRISMA checklist. They had also not received any formal training in preparing systematic reviews. These findings highlight the need to address the knowledge gaps in medical students and junior doctors in performing systematic reviews and meta-analyses.
ADVANCES IN MEDICAL EDUCATION AND PRACTICE
(2023)
Article
Clinical Neurology
Tiam M. Saffari, Kaitlin Kavanagh, Benjamin Ormseth, Marilly Palettas, Jeffrey E. Janis
Summary: This study investigated the relationship between migraine severity, metabolic syndrome, and estrogen-associated variables. The findings showed that severe obesity (BMI > 40) is associated with increased migraine severity and frequency. Post-menopausal patients were also found to have increased migraine frequency.
JOURNAL OF CLINICAL NEUROSCIENCE
(2023)
Article
Orthopedics
Eiji Sasaki, Takeshi Kamitani, Shotaro Kinouchi, Nobuhiro Kamiya, Akira Ikumi, Tomohiko Tateishi, Seiji Miyazaki, Yasuyuki Ishibashi, Shinji Nagahiro
Summary: This study aimed to characterize the age-and sex-specific Anterior cruciate ligament (ACL) injury rates and related injury patterns in judo players in Japan. The results showed that there were differences in the incidence of ACL injuries among different grades and sexes, and the most frequent injury pattern was low-impact contact injury.
ASIA-PACIFIC JOURNAL OF SPORT MEDICINE ARTHROSCOPY REHABILITATION AND TECHNOLOGY
(2023)
Review
Surgery
Jun-Li Tham, Avnish Sood, Tiam Mana Saffari, Ankur Khajuria
Summary: This systematic review evaluates the efficacy of targeted muscle reinnervation (TMR) on pain reduction and functional outcomes in amputees. The analysis of 10 studies shows that TMR can significantly reduce phantom limb pain and residual limb pain, improve quality of life, and have positive effects on limb function for amputees.
EUROPEAN JOURNAL OF PLASTIC SURGERY
(2023)
Review
Cell Biology
Tiam M. Saffari, Sara Saffari, Krishna S. Vyas, Samir Mardini, Alexander Y. Shin
Summary: Autologous fat grafting is commonly used in reconstructive surgery to improve functional form. Fat tissue enrichment with adipose-derived stem cells can increase the long-term stability of grafts. Basic science research suggests that adipose-derived stem cells can enhance nerve regeneration and revascularization, and reduce nerve fibrosis. Clinical studies show promising results in secondary carpal tunnel release revisions using autologous lipofilling.
NEURAL REGENERATION RESEARCH
(2022)
Review
Rheumatology
Akira Ikumi, Shun Okuwaki, Yuki Hara, Yuichi Yoshii, Haruo Kawamura
Summary: This article reports a case of spontaneous extensor pollicis longus (EPL) tendon rupture in a 61-year-old woman with systemic lupus erythematosus (SLE). The tendon rupture may have been caused by a combination of disease-related factors and the long-term use of steroids, chronic inflammation, and mechanical stress from work and daily activities.
MODERN RHEUMATOLOGY CASE REPORTS
(2022)