Review
Oncology
Jiasheng Wang, Leland Metheny
Summary: Cord blood is an important source for allogeneic hematopoietic cell transplantation and is also being developed for cell therapy products. The unique cellular composition of cord blood, including higher percentages of hematopoietic stem cells and progenitor cells, offers advantages in immune tolerance and off-the-shelf cell therapy development. In addition, cord blood-derived mesenchymal stem cells have potential in regenerative medicine and inflammatory/autoimmune conditions. This review focuses on recent clinical development of cord blood-derived therapies in oncology.
FRONTIERS IN ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Se-Hwa Jung, Ji-Eun You, Soon-Won Choi, Kyung-Sun Kang, Je-Yeol Cho, Jungmook Lyu, Pyung-Hwan Kim
Summary: The study investigated the biological function of the PKD1 gene in umbilical cord blood-derived mesenchymal stem cells (UCB-MSC), finding that it can enhance differentiation ability and maintain long-term stemness, providing new possibilities for stem cell therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Juan Wang, Shuang Gao, Yufei Zhao, Taibing Fan, Mingkui Zhang, Dehua Chang
Summary: This study developed culture medium and coating matrix that meet the clinical drug standards for the production of UC-MSC sheets, and also developed a GMP-compliant preservation solution and method. Quality standards for the UC-MSC sheets were formulated according to national and international drug regulations. The production process of UC-MSC sheets on a large scale was standardized, and three batches of trial production were conducted to meet the established quality standards.
Article
Cell & Tissue Engineering
Chuan He, Chao Yang, Qiang Zeng, Zhigang Liu, Fangfang Wang, Qiang Chen, Ting Liu
Summary: Umbilical cord-derived MSCs cultured in different media showed the general characteristics of MSCs. HGF, ANG-1, and TGF-beta 1 secretion by MSCs in the MCL was higher than in the other media, while PGE2 secretion was lower in the MCL. MSCs in the MCL inhibited IFN-gamma and TNF-alpha more effectively. MSC treatment promoted recovery of peripheral blood cells, increased Treg ratio and VEGF level, and significantly improved bone marrow.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Hala M. ElBadre, Sahar E. M. El-Deek, Haidi Karam-Allah Ramadan, Mohamed M. Elbadr, Dina Sabry, Noran M. Ahmed, Amr M. Ahmed, Reham El-Mahdy
Summary: In this study, the effects of umbilical cord mesenchymal stem cells (UC-MSCs) derived exosomes on HepG2 cell line and the underlying mechanism of controlling hepatocellular carcinoma (HCC) proliferation were investigated. The results showed that treatment with exosomes significantly reduced the survival of HepG2 cells and altered the expression levels of SIRT-1, SDF-1, CXCR-4, TNF-α, and caspase-3. Furthermore, the anti-proliferative, apoptotic, and anti-angiogenic effects of exosomes on HepG2 cells were time-dependent. These findings suggest that exosomes could be a potential novel therapy for HCC, but further large-scale studies are needed to confirm this conclusion.
Article
Cell Biology
Santhosh Kacham, Tejal Sunil Bhure, Sindhuja D. Eswaramoorthy, Gaurav Naik, Subha Narayan Rath, Sreenivasa Rao Parcha, Sayan Basu, Virender Singh Sangwan, Sachin Shukla
Summary: Umbilical cord-derived mesenchymal stem cells promote the repair of injured corneal epithelium by stimulating the proliferation of corneal epithelial cells, potentially serving as a non-ocular source of stem cells for treating injury-induced bilateral corneal diseases.
Article
Medicine, General & Internal
Hui-Kuang Huang, Kuang-Kai Hsueh, Yu-Ting Liao, Szu-Hsien Wu, Po-Hsin Chou, Shih-Han Yeh, Jung-Pan Wang
Summary: Infant ADSCs demonstrate superior proliferation rates, antioxidative activity, and differentiation potential compared to UCSCs, with less senescence and replicative stress.
JOURNAL OF THE CHINESE MEDICAL ASSOCIATION
(2023)
Article
Immunology
Mayela Mendt, May Daher, Rafet Basar, Mayra Shanley, Bijender Kumar, Francesca Lim Wei Inng, Sunil Acharya, Hila Shaim, Natalie Fowlkes, Jamie P. Tran, Elif Gokdemir, Nadima Uprety, Ana K. Nunez-Cortes, Emily Ensley, Thao Mai, Lucila N. Kerbauy, Luciana Melo-Garcia, Paul Lin, Yifei Shen, Vakul Mohanty, JunJun Lu, Sufang Li, Vandana Nandivada, Jing Wang, Pinaki Banerjee, Francia Reyes-Silva, Enli Liu, Sonny Ang, April Gilbert, Ye Li, Xinhai Wan, Jun Gu, Ming Zhao, Natalia Baran, Luis Muniz-Feliciano, Jeffrey Wilson, Indreshpal Kaur, Mihai Gagea, Marina Konopleva, David Marin, Guilin Tang, Ken Chen, Richard Champlin, Katayoun Rezvani, Elizabeth J. Shpall
Summary: This study optimized cord blood tissue derived MSCs (CBti MSCs) by priming them with inflammatory cytokines, leading to their metabolic reprogramming and enhanced glycolytic capacity. The metabolically reprogrammed CBti MSCs showed superior immunosuppressive potential, immunomodulatory properties, and homing capabilities, even after cryopreservation and thawing. These primed CBti MSCs significantly improved outcomes in a xenogenic mouse model of GVHD and could serve as a promising therapeutic option for GVHD and other autoimmune disorders.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Shichao Wang, Wenyue Jiang, Shuang Lv, Zhicheng Sun, Lihui Si, Jinxin Hu, Yang Yang, Dingbang Qiu, Xiaobin Liu, Siying Zhu, Lunhao Yang, Ling Qi, Guangfan Chi, Guiqing Wang, Pengdong Li, Baojian Liao
Summary: This study confirms the anti-inflammatory effects of human umbilical cord-derived mesenchymal stem cell exosomes (hUC-MSCs-Exos) in the treatment of chondrocyte inflammation, suggesting their potential as a cell-free treatment strategy for osteoarthritis.
Article
Nanoscience & Nanotechnology
Shijie Tang, Penghong Chen, Haoruo Zhang, Haiyan Weng, Zhuoqun Fang, Caixiang Chen, Guohao Peng, Hangqi Gao, Kailun Hu, Jinghua Chen, Liangwan Chen, Xiaosong Chen
Summary: In this study, the therapeutic effects of hUC-MSC-sEVs on osteoarthritis were compared with hUC-MSCs using proteomics analysis. The results showed that hUC-MSC-sEVs were taken up by chondrocytes and demonstrated great proliferative and migratory capacities. The hUC-MSC-sEVs protected cartilage from damage and upregulated proteins involved in immune regulation and signaling pathways, indicating the potential of hUC-MSC-sEVs as a therapeutic agent for osteoarthritis.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Oncology
Litao Yan, Gejun Liu, Xing Wu
Summary: This study found that exosomal lncRNA H19 derived from UMSCs acts as a competing endogenous RNA to enhance osteochondral activity in chondrocytes by sequestering miR-29b-3p. Injection of exosomes overexpressing H19 promoted sustained cartilage repair, highlighting its potential for developing strategies against cartilage defects.
CLINICAL AND TRANSLATIONAL MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Xiao Zhai, Kai Chen, Huan Yang, Bo Li, Tianjunke Zhou, Haojue Wang, Huipeng Zhou, Shaofeng Chen, Xiaoyi Zhou, Xiaozhao Wei, Yushu Bai, Ming Li
Summary: CD73+ hucMSC-EVs ameliorated inflammation after spinal cord injury by reducing extracellular ATP, promoting A2bR/cAMP/PKA pathway and M2/M1 polarization. They may be promising nano drugs for clinical application in SCI therapy.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Cell Biology
Jia Chen, Yuhan Chen, Xue Du, Guojun Liu, Xiaowei Fei, Jian Ru Peng, Xing Zhang, Fengjun Xiao, Xue Wang, Xiao Yang, Zhichun Feng
Summary: CB-MNCs and UC-MSCs show efficacy in alleviating BPD, with CB-MNCs having more pronounced effects in restoring lung function. Through analysis of lncRNA-miRNA-mRNA and circRNA-miRNA-mRNA networks, it was found that therapeutic effects are related to regulation of GTPase activity, ERK signaling cascade, chromosome regulation, and cell cycle control.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Yufeng Shang, Haotong Guan, Fuling Zhou
Summary: Umbilical cord mesenchymal stem cells (UC-MSCs) have the characteristics of inhibiting tumor cell proliferation and migration, supporting hematopoietic microenvironment, and suppressing the immune system, showing great potential in the treatment of hematologic diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Chemistry, Medicinal
Benjamin J. Main, Nicola Maffulli, Josiah A. Valk, Hugo C. Rodriguez, Manu Gupta, Saadiq F. El-Amin, Ashim Gupta
Summary: Wharton's jelly-derived mesenchymal stem cells (WJSCs) have shown potential therapeutic benefits in orthopedic surgery, but further randomized controlled clinical studies are needed to assess their safety and efficacy.