Article
Biochemistry & Molecular Biology
Cody R. Fisher, Mara C. Ebeling, Zhaohui Geng, Rebecca J. Kapphahn, Heidi Roehrich, Sandra R. Montezuma, James R. Dutton, Deborah A. Ferrington
Summary: This study evaluated the treatment response of haRPE and iPSC-RPE to oxidative stress and potential therapeutics for mitochondrial defects. The results showed disease-specific responses in haRPE, while iPSC-RPE had similar responses regardless of disease status. Analysis of cells generated from the same donor showed a similar response for donors without AMD, but significant differences in treatment response between cell models from AMD donors.
Review
Biochemistry & Molecular Biology
Zhibo Si, Yajuan Zheng, Jing Zhao
Summary: Age-related macular degeneration (AMD) is a common cause of vision loss in the elderly. It is characterized by the formation of Drusen in dry AMD and cell proliferation and choroidal angiogenesis in wet AMD. The retinal pigment epithelium (RPE) plays a crucial role in the development of AMD, with outer segment phagocytosis and autophagy being important processes for RPE cell renewal and protection against oxidative stress damage. However, impaired phagocytosis and autophagy in RPE cells can lead to AMD progression. Understanding the roles of RPE cell phagocytosis and autophagy in AMD pathogenesis can help identify potential therapeutic targets to prevent irreversible RPE and photoreceptor cell death and protect against AMD.
Article
Multidisciplinary Sciences
Pei-Li Yao, Vipul M. Parmar, Mayur Choudhary, Goldis Malek
Summary: The expression and activity of NURR1 play critical roles in the physiological and pathological functions of human RPE cells. Targeting NURR1 may have therapeutic potential for AMD by improving EMT, inflammation, and lipid homeostasis in RPE cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Masahiro Miura, Shuichi Makita, Yoshiaki Yasuno, Takuya Iwasaki, Shinnosuke Azuma, Toshihiro Mino, Tatsuo Yamaguchi
Summary: The study quantitatively evaluated retinal pigment epithelium changes in patients with age-related macular degeneration using prototype multi-contrast optical coherence tomography. The results showed that multi-contrast OCT is useful for objective evaluation of changes in the retinal pigment epithelium.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Jongmin Kim, Yeo Jin Lee, Jae Yon Won
Summary: This review highlights the roles and pathogenesis of retinal pigment epithelium (RPE) in AMD, emphasizing the importance of understanding the etiology of AMD and discussing potential treatment strategies targeting RPE.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, General & Internal
Hyunchae Sim, Wonhwa Lee, Samyeol Choo, Eui Kyun Park, Moon-Chang Baek, In-Kyu Lee, Dong Ho Park, Jong-Sup Bae
Summary: A study evaluated the protective effects of SFN from cruciferous vegetables against PM2.5-induced oxidative damage, showing that SFN effectively alleviates oxidative damage in human ARPE-19 cells via its antioxidant effects.
FRONTIERS IN MEDICINE
(2021)
Article
Chemistry, Medicinal
Shiu-Jau Chen, Tzer-Bin Lin, Hsien-Yu Peng, Hsiang-Jui Liu, An-Sheng Lee, Cheng-Hsien Lin, Kuang-Wen Tseng
Summary: The study demonstrates that fucoxanthin protects RPE cells from oxidative stress-induced premature senescence and decreased photoreceptor cell loss. Fucoxanthin treatment significantly inhibited reactive oxygen species generation and reduced malondialdehyde concentrations in oxidative stress-induced RPE cell damage. Additionally, fucoxanthin may protect against premature senescence and cellular dysfunction in retinal cells exposed to oxidative stress.
Article
Biochemistry & Molecular Biology
Lara Macchioni, Davide Chiasserini, Letizia Mezzasoma, Magdalena Davidescu, Pier Luigi Orvietani, Katia Fettucciari, Leonardo Salviati, Barbara Cellini, Ilaria Bellezza
Summary: Age-related retinal degenerations, such as AMD, are linked to the loss of RPE cells and photoreceptors, involving oxidative stress and inflammatory responses. Long-term oxidative insult can induce senescence in RPE cells, affecting antioxidant response and mitochondrial functionality.
Article
Biochemistry & Molecular Biology
Maria Hytti, Eveliina Korhonen, Heidi Hongisto, Kai Kaarniranta, Heli Skottman, Anu Kauppinen
Summary: The study showed that iPSC-RPE cells from AMD patients exhibit increased secretion of IL-1β and reduced levels of IL-6 and MCP-1 when protein clearance was partially inhibited. This suggests that dysfunctional cellular clearance mechanisms may already be present in cells originating from AMD patients, leading to inflammasome activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Woo Kwon Jung, Su-Bin Park, Hwa Young Yu, Yong Hwan Kim, Junghyun Kim
Summary: Esculetin exerts potent antioxidant effects in ARPE-19 cells, protecting them from oxidative injury induced by t-BHP and reducing apoptosis. These findings suggest the potential therapeutic implications of Esculetin for retinal dysfunction.
Article
Multidisciplinary Sciences
Amrish Selvam, Sumit Randhir Singh, Supriya Arora, Manan Patel, Arnim Kuchhal, Stavan Shah, Joshua Ong, Mohammed Abdul Rasheed, Shanmukh Reddy Manne, Mohammed Nasar Ibrahim, Jose-Alain Sahel, Kiran Kumar Vupparaboina, Jay Chhablani
Summary: We conducted an automated analysis of pigment epithelial detachments (PEDs) in neovascular age related macular degeneration (nAMD) to estimate the areas of serous, neovascular, and fibrous tissues within the PEDs. By manually segmenting the PEDs and filtering the pixels using 2D kernels, we were able to classify them as serous, neovascular, or fibrous. Using specific PED composition indices, we calculated the relative areas of each tissue type within the PEDs. The accuracy of segmentation and classification was graded, and overall repeatability and reproducibility were high.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Ali Koskela, Federico Manai, Filippo Basagni, Mikko Liukkonen, Michela Rosini, Stefano Govoni, Massimo Dal Monte, Adrian Smedowski, Kai Kaarniranta, Marialaura Amadio
Summary: Antioxidant systems play key roles in elderly diseases, including age-related macular degeneration (AMD). This study investigated the protective effects of nature-inspired hybrids on retinal pigment epithelial (RPE) cells. The hybrids increased the expression of Nrf2-target genes and improved intracellular redox balance without affecting autophagy flux. Furthermore, the hybrids enhanced cell survival and reduced inflammation when exposed to proteasome and autophagy inhibitors. The study suggests that Nrf2 is a valuable target for treating AMD.
Article
Cell Biology
Ha Young Jang, Chang Sik Cho, Young Mi Shin, Jina Kwak, Young Hoon Sung, Byeong-Cheol Kang, Jeong Hun Kim
Summary: This study investigated the mechanisms underlying retinal pigment epithelium dysfunction in aging and age-related macular degeneration (AMD) using primary marmoset cells. Comparisons were made between the effects of fetal bovine serum (FBS) and serum-free supplements on cell characteristics, and age-related morphological changes were assessed. The results suggest that primary marmoset RPE cells provide a reliable in vitro model for developing therapeutics for AMD.
Article
Ophthalmology
Gang Shen, Yanmei Li, Yongcheng Zeng, Fuyan Hong, Jing Zhang, Yan Wang, Chengwei Zhang, Wei Xiang, Jinhong Wang, Zhenzhen Fang, Weiwei Qi, Xia Yang, Guoquan Gao, Ti Zhou
Summary: KAL inhibits oxidative stress-induced epithelial-mesenchymal transition (EMT) in retinal pigment epithelium (RPE) by downregulating the transcription factor Snail. KAL knockout rats can serve as an appropriate animal model for observing spontaneous RPE dysfunction for dry age-related macular degeneration (AMD)-like retinopathy, and KAL may represent a novel therapeutic target for treating dry AMD.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Sofia Ranta-aho, Niina Piippo, Eveliina Korhonen, Kai Kaarniranta, Maria Hytti, Anu Kauppinen
Summary: The study found that TAS-116 could prevent the activation of caspase-1, subsequently reducing the release of mature IL-1 beta. TAS-116 has a better in vitro therapeutic index than geldanamycin. In summary, TAS-116 appears to be a well-tolerated Hsp90 inhibitor, with the capability to prevent the activation of the NLRP3 inflammasome in human RPE cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Millie M. Georgiadis, Qiujia Chen, Jingwei Meng, Chunlu Guo, Randall Wireman, April Reed, Michael R. Vasko, Mark R. Kelley
Article
Oncology
Derek P. Logsdon, Michelle Grimard, Meihua Luo, Safi Shahda, Yanlin Jiang, Yan Tong, Zhangsheng Yu, Nicholas Zyromski, Ernestina Schipani, Fabrizio Carta, Claudiu T. Supuran, Murray Korc, Mircea Ivan, Mark R. Kelley, Melissa L. Fishel
MOLECULAR CANCER THERAPEUTICS
(2016)
Article
Oncology
Jixin Ding, Melissa L. Fishel, April M. Reed, Erin McAdams, Magdalena B. Czader, Angelo A. Cardoso, Mark R. Kelley
MOLECULAR CANCER THERAPEUTICS
(2017)
Article
Oncology
Fenil Shah, Emery Goossens, Nadia M. Atallah, Michelle Grimard, Mark R. Kelley, Melissa L. Fishel
MOLECULAR ONCOLOGY
(2017)
Editorial Material
Cell Biology
Mark R. Kelley, Jill C. Fehrenbacher
NEURAL REGENERATION RESEARCH
(2017)
Article
Neurosciences
Jill C. Fehrenbacher, Chunlu Guo, Mark R. Kelley, Michael R. Vasko
Article
Geriatrics & Gerontology
Tao Yan, Poornima Venkat, Michael Chopp, Alex Zacharek, Peng Yu, Ruizhuo Ning, Xiaoxi Qiao, Mark R. Kelley, Jieli Chen
Article
Pharmacology & Pharmacy
Sheik Pran Babu Sardar Pasha, Kamakshi Sishtla, Rania S. Sulaiman, Bomina Park, Trupti Shetty, Fenil Shah, Melissa L. Fishel, James H. Wikel, Mark R. Kelley, Timothy W. Corson
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2018)
Article
Multidisciplinary Sciences
Derek P. Logsdon, Fenil Shah, Fabrizio Carta, Claudiu T. Supuran, Malgorzata Kamocka, Max H. Jacobsen, George E. Sandusky, Mark R. Kelley, Melissa L. Fishel
SCIENTIFIC REPORTS
(2018)
Article
Cell & Tissue Engineering
Zhigang Cai, Jonathan J. Kotzin, Baskar Ramdas, Sisi Chen, Sai Nelanuthala, Lakshmi Reddy Palam, Ruchi Pandey, Raghuveer Singh Mali, Yan Liu, Mark R. Kelley, George Sandusky, Morvarid Mohseni, Adam Williams, Jorge Henao-Mejia, Reuben Kapur
Article
Chemistry, Medicinal
Richard Trilles, Dmitri Beglov, Qiujia Chen, Hongzhen He, Randall Wireman, April Reed, Spandan Chennamadhavuni, James S. Panek, Lauren E. Brown, Sandor Vajda, John A. Porco, Mark R. Kelley, Millie M. Georgiadis
JOURNAL OF MEDICINAL CHEMISTRY
(2019)
Article
Oncology
Melissa Fishel, Hanyu Xia, Jack McGeown, David W. McIlwain, May Faek Mohamed Elbanna, Ariel A. Craft, Hristos Z. Kaimakliotis, George Sandusky, Chi Zhang, Roberto Pili, Mark R. Kelley, Travis J. Jerde
MOLECULAR CANCER THERAPEUTICS
(2019)
Article
Genetics & Heredity
Marta Codrich, Marina Comelli, Matilde Clarissa Malfatti, Catia Mio, Dilara Ayyildiz, Chi Zhang, Mark R. Kelley, Giovanni Terrosu, Carlo Em Pucillo, Gianluca Tell
Article
Oncology
Charles L. Loprinzi, Christina Lacchetti, Jonathan Bleeker, Guido Cavaletti, Cynthia Chauhan, Daniel L. Hertz, Mark R. Kelley, Antoinette Lavino, Maryam B. Lustberg, Judith A. Paice, Bryan P. Schneider, Ellen M. Lavoie Smith, Mary Lou Smith, Thomas J. Smith, Nina Wagner-Johnston, Dawn L. Hershman
JOURNAL OF CLINICAL ONCOLOGY
(2020)
Article
Pharmacology & Pharmacy
Mark R. Kelley, James H. Wikel, Chunlu Guo, Karen E. Pollok, Barbara J. Bailey, Randy Wireman, Melissa L. Fishel, Michael R. Vasko
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2016)