Article
Biology
Xianrong Xie, Huilong Du, Huiwu Tang, Jianian Tang, Xiyu Tan, Weizhi Liu, Tie Li, Zhansheng Lin, Chengzhi Liang, Yao-Guang Liu
Summary: This study successfully generated a high-quality genome assembly of Oryza rufipogon, revealing insights into the genomic structure of the wild rice, its homology with cultivated rice, and the polyphyletic origins of O. sativa subspecies.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Pavneet Kaur, Kumari Neelam, Preetinder Singh Sarao, Ankita Babbar, Kishor Kumar, Yogesh Vikal, Renu Khanna, Rupinder Kaur, Gurjeet Singh Mangat, Kuldeep Singh
Summary: This study successfully identified and transferred a novel BPH resistance gene, bph42, from wild rice species into elite rice cultivars. Through genetic mapping and QTL analysis, the location of this resistance gene on chromosome 4 was determined, providing important insights for future varietal release. The identified marker associated with bph42 can be efficiently used in marker-assisted selection for breeding BPH-resistant rice cultivars.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Yibo Wang, Xin Yang, Guimin Xu, Xiao Ye, Yiming Ji, Xin Lou, Jingjing Su, Chuanqing Sun, Yongcai Fu, Kun Zhang
Summary: By using introgression lines and SSR markers, we identified 30 QTLs associated with callus browning in indica rice. These QTLs showed additive effects in reducing callus browning and two novel QTLs were found on chromosome 12. These findings provide insight into improving tissue culturability in indica rice.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Plant Sciences
Jing Zhang, Dajian Pan, Zhilan Fan, Hang Yu, Liqun Jiang, Shuwei Lv, Bingrui Sun, Wenfeng Chen, Xingxue Mao, Qing Liu, Chen Li
Summary: The study developed 1,592 SNPs for genetic analysis of 998 O. rufipogon accessions from Guangdong and Hainan Provinces in China. The results showed that O. rufipogon accessions had rich genetic diversity, with variations observed among different regions. A core collection was constructed based on genetic distance, capturing over 99% of the genetic variation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Parthiban Thathapalli Prakash, Dmytro Chebotarov, Jianwei Zhang, David A. Kudrna, Rolando O. Torres, Mignon A. Natividad, Marinell R. Quintana, Jiaming Song, Carlos E. Maldonado, Sherry Lou Hechanova, Kshirod Jena, Rod A. Wing, Amelia Henry
Summary: Developing drought-resistant rice is crucial for improving field productivity. Wild relatives of rice have potential traits for drought resistance. This study identified Oryza glumaepatula as a promising source for drought resistance. Multiple genomic loci related to drought resistance traits were detected through whole-genome resequencing and variant calling. These findings provide valuable information for introgressing drought tolerance into O. sativa backgrounds.
Article
Plant Sciences
Jun Hong, Leah Rosental, Yang Xu, Dawei Xu, Isabel Orf, Wengsheng Wang, Zhiqiang Hu, Su Su, Shaoxing Bai, Mohammed Ashraf, Chaoyang Hu, Changquan Zhang, Zhikang Li, Jianlong Xu, Qiaoquan Liu, Hui Zhang, Fengli Zhang, Zhijing Luo, Mingjiao Chen, Xiaofei Chen, Natalie Betts, Alisdair Fernie, Wanqi Liang, Guanqun Chen, Yariv Brotman, Dabing Zhang, Jianxin Shi
Summary: This study revealed the genetic regulation of rice seed glycerolipids using metabolite-based approaches. Two genes were found to be involved in the variation of saturated triacylglycerol and lyso-phosphatidylcholine contents. The study also demonstrated the association of these genes with other traits such as heading date and flooding tolerance. The findings suggest that the diversity of rice seed glycerolipids may have been unintentionally shaped during natural and artificial selection.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Multidisciplinary Sciences
Ryo Ishikawa, Cristina Cobo Castillo, Than Myint Htun, Koji Numaguchi, Kazuya Inoue, Yumi Oka, Miki Ogasawara, Shohei Sugiyama, Natsumi Takama, Chhourn Orn, Chizuru Inoue, Ken-Ichi Nonomura, Robin Allaby, Dorian Q. Fuller, Takashige Ishii
Summary: This study reveals the process of early rice domestication and demonstrates that the reduction of seed shattering is not solely controlled by the sh4 allele but requires the intervention of the qSH3 allele as well. The study also identifies the impact of qSH3 within the seed shattering gene OsSh1, which is consistent in the indica and japonica subspecies but absent in the circum-aus group of rice. Furthermore, the study shows that seed shattering alone does not significantly impact yield, but yield increases are observed with closed panicle formation controlled by SPR3 and further augmented by nonshattering conferred by integration of sh4 and qSH3 alleles.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Neng Zhao, Ruizhi Yuan, Babar Usman, Jiaming Qin, Jinlian Yang, Liyun Peng, Enerand Mackon, Fang Liu, Baoxiang Qin, Rongbai Li
Summary: Wild rice is a valuable source for genes that can be used in breeding rice cultivars with beneficial traits. Chromosome segment substitution lines (CSSLs) provide a powerful tool for mapping quantitative trait loci (QTLs). In this study, 59 CSSLs were developed through backcrossing and marker-assisted selection (MAS) using common wild rice (Oryza rufipogon Griff.) as the donor and indica rice variety 93-11 as the recipient. Whole genome re-sequencing identified numerous molecular markers for these CSSLs, and through mapping analysis, QTLs related to economic traits were identified. The findings of this study have important implications for theoretical knowledge and genetic applications in rice breeding.
Article
Biochemistry & Molecular Biology
Wanling Yang, Yong Chen, Rifang Gao, Yaling Chen, Yi Zhou, Jiankun Xie, Fantao Zhang
Summary: This study reveals that miR2871b in DXWR negatively regulates cold and salt stress tolerance. Overexpression of miR2871b increases sensitivity of transgenic rice plants to cold and salt stresses, reducing their tolerance. Furthermore, increased miR2871b leads to accumulation of ROS and MDA, and decreases activities of POD, SOD, CAT, as well as contents of Pro and SS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Li-Ying Feng, Pei-Fan Lin, Rong-Jing Xu, Hai-Qi Kang, Li-Zhi Gao
Summary: This study provides a comprehensive analysis of the PPR gene family in rice and its wild progenitor, revealing the landscape of gene duplications and the potential role of segmental duplications in promoting the structural diversity of PPR genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agronomy
P. S. Basavaraj, Bharamappanavara Muralidhara, C. A. Manoj, M. S. Anantha, Santosha Rathod, Ch Damodar Raju, P. Senguttuvel, M. S. Madhav, M. Srinivasaprasad, V Prakasam, K. Basavaraj, Jyothi Badri, L. Subbarao, R. M. Sundaram, C. Gireesh
Summary: The study focused on enhancing the elite rice cultivar 'Samba Mahsuri' by using wild rice species Oryza rufipogon, resulting in the identification of several introgression lines with superior yield-enhancing traits and blast resistance. Molecular characterization revealed multiple blast resistance genes in some of the introgression lines. These promising lines with high yield potential and resistance to blast disease can serve as valuable genetic resources for rice improvement in Southern India.
GENETIC RESOURCES AND CROP EVOLUTION
(2021)
Article
Plant Sciences
Sun Ha Kim, Kyu-Chan Shim, Hyun-Sook Lee, Yun-A Jeon, Cheryl Adeva, Ngoc Ha Luong, Sang-Nag Ahn
Summary: This study uncovers the association of OsD2 gene with low-temperature germinability (LTG) and grain size in rice. Overexpression of OsD2 leads to an increase in LTG and grain size, while knockdown of OsD2 results in a decrease in these traits. These findings suggest that OsD2 may serve as a suitable target for rice breeding programs aimed at improving yield and LTG.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Cheng Huang, Jilin Wang, Dianwen Wang, Jingjing Chang, Hongping Chen, Dazhou Chen, Wei Deng, Chunjie Tian
Summary: In this study, six OsSPXs were identified from the whole genome of DXWR and found to be highly sensitive to cold stress. The promoter region of DXWR OsSPXs contains cis-acting elements related to abiotic stress tolerance and plant hormone response. This study provides useful information for gene-function research and genetic improvements in DXWR breeding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Richard S. Garcia, Sapphire Coronejo, Jonathan Concepcion, Prasanta K. Subudhi
Summary: This study reveals the presence of specific flowering genes and unique variants in the photoinsensitive weedy rice accession PSRR-1. OsMADS56 is suggested as a key regulator for its day-neutral flowering response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agronomy
Ran Xu, Chuanqing Sun
Summary: Crop domestication has been a key factor in human civilization progress, and understanding the genetic mechanisms underlying the differences between wild and cultivated rice can help advance crop breeding efforts and food production.