Article
Engineering, Biomedical
Caleb Sponheim, Vasileios Papadourakis, Jennifer L. Collinger, John Downey, Jeffrey Weiss, Lida Pentousi, Kaisa Elliott, Nicholas G. Hatsopoulos
Summary: Microelectrode arrays, specifically Utah electrode arrays (UEAs), are widely used in chronic electrophysiological experiments for recording neural activity. This study evaluated the reliability and longevity of UEAs over almost nine years in 17 rhesus macaques and 2 human subjects, finding an average lifespan of 622 days with some arrays lasting over 1000 days and one up to 9 years. Results suggest chronic unit recordings can last at least two years, with some arrays potentially lasting up to a decade.
JOURNAL OF NEURAL ENGINEERING
(2021)
Article
Engineering, Biomedical
Tianshu Dong, Lei Chen, Paras R. Patel, Julianna M. Richie, Cynthia A. Chestek, Albert J. Shih
Summary: The machine described in this study automates the assembly of high-density carbon fiber (HDCF) arrays, eliminating the need for manual labor and improving accuracy and repeatability. The machine includes a roller-based carbon fiber extruder, a motion system with linear and rotary stages, an imaging system with digital microscope cameras, and a laser cutter. It is capable of precisely handling carbon fiber electrodes (CFEs) and accurately placing them on silicon support shanks. The use of this machine is significant for achieving fully automated HDCF array assembly and batch production.
JOURNAL OF NEURAL ENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
Pegah Shokoohimehr, Bogdana Cepkenovic, Frano Milos, Justus Bednar, Hossein Hassani, Vanessa Maybeck, Andreas Offenhaeusser
Summary: The further development of neurochips requires high-density and high-resolution recordings over a long period of time. A combination of vertical nanostraws with nanocavities is proposed, allowing tight coupling with neurons and achieving high sensitivity and synaptic resolution in recording.
Article
Biochemical Research Methods
Samantha P. Sherrill, Nicholas M. Timme, John M. Beggs, Ehren L. Newman
Summary: Our study indicates that the ability of synergistic integration in neural networks is influenced by the directionality of local information flow. Greater synergistic integration occurs where there is more recurrent information flow, while lesser synergistic integration occurs where there is more feedback information flow relative to feedforward information flow.
PLOS COMPUTATIONAL BIOLOGY
(2021)
Article
Neurosciences
Lei Dong, Lin-Lin Song, Wen-jun Zhao, Ling Zhao, Lei Tian, Yu Zheng
Summary: Electromagnetic stimulation (EMS) can modulate the transition of epileptiform activity (EFA) from brief interictal discharges (IIDs) to prolonged ictal discharges (IDs). The study found that real-time magnetic fields can block the normal EFA process and this suppression effect depends on input intensities and intervention duration.
BRAIN RESEARCH BULLETIN
(2023)
Article
Physics, Multidisciplinary
Javier G. Orlandi, Jaume Casademunt
Summary: Recent experiments introduce a dynamical model called stochastic quorum percolation to explain the spontaneous activity of developing dissociated neuronal cultures. The model reproduces first- and second-order phase transitions in bursting dynamics and neuronal avalanches, and highlights the impact of metric correlations in network topology on dynamics.
Article
Engineering, Biomedical
Yingying Wang, Lelun Jiang, Lei Ren, Pingao Huang, Mei Yu, Lan Tian, Xiangxin Li, Jun Xie, Peng Fang, Guanglin Li
Summary: The use of microneedle electrode array improves the quality of electrophysiological signal recordings on human body surface with lower interface impedance and more stable signal recording characteristics. It enables high-quality electromyography and electrocardiography recordings, without causing discomfort. Microneedles penetrate through the corneum and reach the epidermis to fix electrodes on the body surface for high-quality signal recording, especially during body movements.
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
(2021)
Article
Acoustics
Benjamin Yen, Yusuke Hioka, Gian Schmid, Brian Mace
Summary: This study proposes a method to perform sound source enhancement from an unmanned aerial vehicle (UAV)mounted audio recording system, utilizing audio recordings and non-acoustical UAV rotor characteristics to improve rotor noise power spectral density estimation accuracy. The experiment results show significant improvement in source enhancement performance and high PSD estimation accuracy under different input conditions.
Article
Biochemistry & Molecular Biology
Maria Jose Gonzalez-Riquelme, Fernando Lucas-Ruiz, Caridad Galindo-Romero, Raquel Boia, Antonio Francisco Ambrosio, Manuel Vidal-Sanz, Ana Raquel Santiago, Marta Agudo-Barriuso
Summary: This study compares retinal organotypic cultures (ROCs) with in vivo models to study retinal ganglion cell (RGC) death and glial activation. The results show that there are significant differences in glial activation between ROCs and in vivo models, with earlier and different morphological activation in ROCs. Additionally, microglial cell density in ROCs is consistently lower than in vivo. ROCs can be used for proof-of-concept studies on neuroprotection, but long-term experiments should be performed in vivo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Konstantinos Gkatzis, Paolo Panza, Sofia Peruzzo, Didier Y. R. Stainier
Summary: This study reports serum-free conditions that allow for growth and differentiation of mouse distal lung epithelial progenitors. Collagen I was found to promote the differentiation of flattened, polarized AT1 cells, and a chemical screen was conducted to investigate the relationship between WNT signaling and epithelial differentiation. An association between Casein Kinase activity and maintenance of an AT2 expression signature was identified, with Casein Kinase inhibition leading to an increase in AT1/progenitor cell ratio. These organoids provide a simplified model of alveolar differentiation and constitute a scalable screening platform for identifying and analyzing cell differentiation mechanisms.
Article
Biochemical Research Methods
Ines C. Bras, Mohammad H. Khani, Dietmar Riedel, Iwan Parfentev, Ellen Gerhardt, Christoph van Riesen, Henning Urlaub, Tim Gollisch, Tiago F. Outeiro
Summary: EVs are important mediators in intercellular communication. This study characterizes the proteomes of ectosomes and exosomes, establishes specific protein markers for their distinction, and demonstrates their effects on neuronal activity.
JOURNAL OF PROTEOMICS
(2022)
Article
Pharmacology & Pharmacy
Qianyu Chen, Shenna Langenbach, Meina Li, Yuxiu C. Xia, Xumei Gao, Matthew J. Gartner, Elizabeth A. Pharo, Sinead M. Williams, Shawn Todd, Nadeene Clarke, Sarath Ranganathan, Michelle L. Baker, Kanta Subbarao, Alastair G. Stewart
Summary: This study established a physiologically relevant airway epithelial model for investigating therapeutics for COVID-19. ACE2 was mainly localized in ciliated and basal epithelial cells, and asthma and smoking status had no consistent marked influence on ACE2 expression or distribution.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Lin He, Chuxia Deng
Summary: Organotypic tissue slice culture has numerous advantages in anticancer drug development, including the preservation of cell repertoire, quick assessment of therapeutic efficacy, and the ability to select personalized treatment regimens. It is expected to become a potent platform for individualized drug screening.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Dhwani S. Korde, Christian Humpel
Summary: This study investigates the spreading of P301S aggregated tau in the brain using organotypic slice cultures and collagen hydrogels as a protein delivery system. The results show that P301S aggregated tau spreads to different areas of the brain in a time-dependent manner, and this spreading is interrupted when the neuroanatomical pathways are lesioned. The study provides a novel experimental approach to investigate tau pathology.
Article
Pharmacology & Pharmacy
Ander Egimendia, Susana Carregal-Romero, Inaki Osorio-Querejeta, Daniel Padro, Jesus Ruiz-Cabello, David Otaegui, Pedro Ramos-Cabrer
Summary: Ex vivo models using multimodal imaging and myelin-targeting liposomes show potential for quantifying myelin content in organotypic cultures. MRI can successfully image brain tissue sections with good contrast, while myelin-targeting liposomes allow for semiquantitative detection by fluorescence. Fine tuning of the probe is necessary to improve specificity when using lipid-based systems.
Article
Neurosciences
Christian Meisel, Andreas Klaus, Vladyslav V. Vyazovskiy, Dietmar Plenz
JOURNAL OF NEUROSCIENCE
(2017)
Article
Biochemical Research Methods
Wesley P. Clawson, Nathaniel C. Wright, Ralf Wessel, Woodrow L. Shew
PLOS COMPUTATIONAL BIOLOGY
(2017)
Article
Biochemical Research Methods
Andrea K. Barreiro, Shree Hari Gautam, Woodrow L. Shew, Cheng Ly
PLOS COMPUTATIONAL BIOLOGY
(2017)
Article
Psychiatry
Saurav Seshadri, Andreas Klaus, Daniel E. Winkowski, Patrick O. Kanold, Dietmar Plenz
TRANSLATIONAL PSYCHIATRY
(2018)
Article
Multidisciplinary Sciences
Leila Fakhraei, Shree Hari Gautam, Woodrow L. Shew
Article
Mathematics, Applied
Vidit Agrawal, Andrew B. Cowley, Qusay Alfaori, Daniel B. Larremore, Juan G. Restrepo, Woodrow L. Shew
Article
Multidisciplinary Sciences
Patrick A. Kells, Shree Hari Gautam, Leila Fakhraei, Jingwen Li, Woodrow L. Shew
NATURE COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Tazima Nur, Shree Hari Gautam, Julie A. Stenken, Woodrow L. Shew
SCIENTIFIC REPORTS
(2019)
Editorial Material
Neurosciences
Douglas A. Roisen, Woodrow L. Shew
JOURNAL OF PHYSIOLOGY-LONDON
(2020)
Article
Biochemical Research Methods
Jingwen Li, Woodrow L. Shew
PLOS COMPUTATIONAL BIOLOGY
(2020)
Article
Biochemical Research Methods
Michelle F. Craft, Andrea K. Barreiro, Shree Hari Gautam, Woodrow L. Shew, Cheng Ly
Summary: Most olfaction studies have focused on orthonasal stimulation, where odors enter through the front nasal cavity, while retronasal olfaction, where odors enter through the rear nasal cavity during feeding, has been understudied. Using in vivo recordings of rat mitral cells in the olfactory bulb in response to a food odor, this study found significant differences in firing rates and spike count covariances between orthonasal and retronasal stimulation. These differences in activity may ultimately shape how odors from different pathways are coded in the brain.
PLOS COMPUTATIONAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Jingwen Li, Patrick A. Kells, Ayla C. Osgood, Shree Hari Gautam, Woodrow L. Shew
Summary: The complex dynamics and coordination among neurons in the motor cortex are important for complex body movements. Excessive inhibition can lead to excessive synchrony among movement-related neurons, resulting in a collapse of complexity in the motor system and simpler behavior. This imbalance of inhibition may underlie abnormal motor function in conditions like Rett syndrome.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Sabrina A. Jones, Jacob H. Barfield, V. Kindler Norman, Woodrow L. Shew
Summary: Naturally occurring body movements and collective neural activity exhibit scale-free dynamics, with fractal spatiotemporal structure. Scale-free brain activity and scale-free behavior have been studied separately, without a unified explanation. We show that mouse behavior and neurons in the visual cortex exhibit strongly related scale-free dynamics, with stochastic competition among neural subsets. Our computational model incorporating cell-type-specific circuit structure provides a new explanation for these findings, establishing the neural underpinnings of scale-free behavior and the relevance of scale-free neural activity to behavior.
Article
Physics, Multidisciplinary
Kathleen Finlinson, Woodrow L. Shew, Daniel B. Larremore, Juan G. Restrepo
PHYSICAL REVIEW RESEARCH
(2020)
Article
Multidisciplinary Sciences
Vidit Agrawal, Srimoy Chakraborty, Thomas Knopfel, Woodrow L. Shew