Article
Biochemistry & Molecular Biology
Ikumi Fujita, Akatsuki Kimura, Akira Yamashita
Summary: In this study, it was found that fission yeast undergoes premeiotic nuclear oscillation, which is dependent on microtubules and is driven by cytoplasmic dynein. The oscillation frequency is cell length-dependent and is controlled by a balance between microtubule and viscous drag forces, as well as microtubule dynamics. These findings provide a basis for understanding the physical properties of microtubule-dependent nuclear movements.
Review
Cell Biology
Giampiero Cai
Summary: The pollen tube plays a fundamental role in the reproduction of seed plants. While there is a considerable amount of information available for the growth and signal sensing mechanism of pollen tubes, the role of microtubules in organelle transport remains uncertain. This review postulates possible roles of kinesins in the pollen tube based on information obtained from other plant cells.
Article
Multidisciplinary Sciences
Rotimi Fasimoye, Wentao Dong, Raja S. Nirujogi, Eshaan S. Rawat, Miharu Iguchi, Kwamina Nyame, Toan K. Phung, Enrico Bagnoli, Alan R. Prescott, Dario R. Alessi, Monther Abu-Remaileh
Summary: The Golgi is a crucial organelle involved in protein and lipid biosynthesis, as well as sorting and secretion. Dysfunctional Golgi is associated with various diseases. This study presents a Golgi immunoprecipitation technique to isolate intact Golgi mini-stacks for analysis. The analysis pipeline reveals the molecular features of the human Golgi, including proteome, metabolome, and lipidome profiles. This comprehensive molecular map provides a powerful method to study the Golgi in health and disease.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Miao Lan, Xianan Liu, Erfang Kang, Ying Fu, Lei Zhu
Summary: Microtubulin dynamics and organization are crucial for the development and morphogenesis of plant cells. The motor protein kinesins, which are based on microtubules, are primarily responsible for the transport of organelles and vesicles, while some also regulate microtubule organization. ARK family, a plant-specific motor protein subfamily, has three members in Arabidopsis thaliana (ARK1, ARK2, and ARK3). It has been shown that ARK2 is involved in root epidermal cell morphogenesis, but its association with microtubules and the underlying mechanism need further exploration.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Allison Ogren, Sneha Parmar, Soumya Mukherjee, Samuel J. Gonzalez, Melissa Plooster, Mark McClellan, Anirudh G. Mannava, Elliott Davidson, Trisha N. Davis, Melissa K. Gardner
Summary: Kinesin-14 motors play a role in controlling microtubule lengths by interacting with microtubule plus-ends and walking towards the minus-end. The binding of Kinesin-14 motors to microtubule plus-ends results in a decrease in microtubule lifetime and elongation, while inhibition of Kinesin-14 minus-end-directed motility leads to extended interactions and stabilization of microtubule plus-ends. This study demonstrates the importance of Kinesin-14 motors in regulating microtubule dynamics.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Agata Gluszek-Kustusz, Benjamin Craske, Thibault Legal, Toni McHugh, Julie P. Welburn
Summary: During mitosis, the microtubule crosslinker protein PRC1 recruits motor proteins CENP-E and Kif4A to the central spindle to coordinate chromosome segregation and cytokinesis. This recruitment is mediated by a conserved hydrophobic motif and is spatially and temporally regulated by CENP-E. The interaction between PRC1 and microtubule motor proteins is essential for controlling chromosome partitioning, maintaining central spindle integrity, and ensuring cytokinesis during anaphase.
Review
Biochemistry & Molecular Biology
Christian Covill-Cooke, Viktoriya S. Toncheva, Josef T. Kittler
Summary: Peroxisomes are essential organelles involved in metabolic processes, requiring both long- and short-range trafficking for optimal positioning in cells. This review examines the mechanisms of peroxisomal distribution, with a focus on regulatory overlaps between mitochondrial and peroxisomal trafficking, as well as the role of mitochondrial Rho-GTPase Miro in peroxisomal dynamics. Additionally, it discusses pathological and pharmacological conditions affecting peroxisomal positioning, along with highlighting gaps in current knowledge and suggesting future research directions.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Cell Biology
Chengye Feng, Joseph M. Cleary, Gregory O. Kothe, Michelle C. Stone, Alexis T. Weiner, James Hertzler, William O. Hancock, Melissa M. Rolls
Summary: Axons and dendrites are distinguished by microtubule polarity, with dendrites containing minus-end-out microtubules and axons having plus-end-out microtubules. Trim9 and kinesin-5 work together at microtubule plus ends to promote new microtubule growth and resist catastrophe. Successful exit of microtubules at branch points depends on their orientation and parallel orientation with other microtubules.
JOURNAL OF CELL SCIENCE
(2021)
Article
Cell Biology
Patrik Risteski, Domagoj Bozan, Mihaela Jagric, Agneza Bosilj, Nenad Pavin, Iva M. Tolic
Summary: This study discovered the importance of chromosome alignment at the spindle equator for proper chromosome segregation, which relies on the pulling forces exerted at kinetochore fiber tips and polar ejection forces. Additionally, the study found that the forces driving the poleward flux of kinetochore fibers also affect chromosome alignment. A flux-driven centering model was introduced, which depends on the flux generated by forces within the overlaps of bridging and kinetochore fibers. The study confirmed the key prediction that kinetochore fiber flux is length dependent. Several regulators of overlap and flux were identified, including Kif18A, Kif4A, and NuMA.
Article
Physics, Multidisciplinary
Linnea M. Lemma, Michael M. Norton, Alexandra M. Tayar, Stephen J. DeCamp, S. Ali Aghvami, Seth Fraden, Michael F. Hagan, Zvonimir Dogic
Summary: The study quantified the motion of microtubule bundles, revealing that the extension speed of isolated bundles is faster than that in dense active nematics. In the dense active nematic, the sliding speeds of filaments are widely distributed, showing both contractile and extensile relative motion.
PHYSICAL REVIEW LETTERS
(2021)
Article
Developmental Biology
Naoki Minamino, Takuya Norizuki, Shoji Mano, Kazuo Ebine, Takashi Ueda
Summary: Gametogenesis is a crucial process in sexual reproduction, and the sperm development in bryophytes involves intricate cellular reorganization. This study classified the developmental processes during sperm formation in the liverwort Marchantia polymorpha based on changes in cellular and nuclear shapes, as well as flagellar development. The researchers also investigated the remodeling of microtubules and the reorganization of endomembrane organelles. The findings revealed changes in the glutamylation of tubulin during flagella and spline formation, and the precise and regulated reorganization of plasma membrane and endomembrane organelles involving the functions of endosomal sorting complexes required for transport (ESCRT) machineries. These findings provide valuable insights for the classification of sperm development in bryophytes.
Article
Multidisciplinary Sciences
Ibtissem Nabti, Babu J. N. Reddy, Rachid Rezgui, Wenqi Wang, Steven P. Gross, George T. Shubeita
Summary: This study demonstrates that phosphorylated MAP4 can bias organelle transport towards the microtubule minus-ends by impairing the force generation of kinesin-1.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Jingjing Fang, Yan Chun, Tingting Guo, Mengmeng Ren, Jinfeng Zhao, Xueyong Li
Summary: The study found that STD1 specifically interacts with MAP65-5 in rice, and they cooperatively control the formation of microtubule bundles during phragmoplast expansion in cell division. Microtubules play crucial roles in the plant cell cycle progression. STD1, a kinesin-related protein, was previously identified as a regulator of phragmoplast expansion in rice. However, the mechanism by which STD1 regulates microtubule organization was unclear. The researchers discovered that STD1 directly interacts with MAP65-5, a microtubule-associated protein, and their interaction enhances microtubule bundling in the phragmoplast.
Article
Biology
Karen M. Page, Jessica J. McCormack, Mafalda Lopes-da-Silva, Francesca Patella, Kimberly Harrison-Lavoie, Jemima J. Burden, Ying-Yi Bernadette Quah, Dominic Scaglioni, Francesco Ferraro, Daniel F. Cutler
Summary: In this study, a hypothetical Golgi organization was validated against quantifiable secretory output. The best-fitting model suggests a highly dynamic ribbon structure with stable mini-stack dimers, capable of rapid rearrangement. This model indicates that the Golgi ribbon is more complex than generally thought.
Review
Biochemistry & Molecular Biology
Meilan Chen, Lu Xu, Yi Wu, Peter Soba, Chun Hu
Summary: Dendrites are specialized compartments in neurons that sense, integrate, and transfer information. Abnormal dendrite development is strongly linked to neurological disorders. The Golgi apparatus, located at the center of the secretory pathway, plays a crucial role in posttranslational modifications, sorting, transport, and signal transduction. It also forms distinct structures known as Golgi outposts in dendrites. However, the organization and function of Golgi in dendrite development and its impact on neurological disorders are still not well understood and lack a comprehensive summary.
Article
Plant Sciences
Luigi Parrotta, Claudia Faleri, Stefano Del Duca, Giampiero Cai
Article
Agricultural Engineering
Aurelie Backes, Marc Behr, Xuan Xu, Edoardo Gatti, Sylvain Legay, Stefano Predieri, Jean-Francois Hausman, Michael K. Deyholos, Giampiero Cai, Gea Guerriero
INDUSTRIAL CROPS AND PRODUCTS
(2018)
Article
Plant Sciences
Lavinia Mareri, Marco Romi, Giampiero Cai
Article
Chemistry, Multidisciplinary
Fabio Candotto Carniel, Davide Gorelli, Emmanuel Flahaut, Lorenzo Fortuna, Cecilia Del Casino, Giampiero Cai, Massimo Nepi, Maurizio Prato, Mauro Tretiach
ENVIRONMENTAL SCIENCE-NANO
(2018)
Review
Genetics & Heredity
Gea Guerriero, Roberto Berni, J. Armando Munoz-Sanchez, Fabio Apone, Eslam M. Abdel-Salam, Ahmad A. Qahtan, Abdulrahman A. Alatar, Claudio Cantini, Giampiero Cai, Jean-Francois Hausman, Khawar Sohail Siddiqui, S. M. Teresa Hernandez-Sotomayor, Mohammad Faisal
Review
Biochemistry & Molecular Biology
Roberto Berni, Claudio Cantini, Marco Romi, Jean-Francois Hausman, Gea Guerriero, Giampiero Cai
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Review
Plant Sciences
Roberto Berni, Marie Luyckx, Xuan Xu, Sylvain Legay, Kjell Sergeant, Jean-Francois Hausman, Stanley Lutts, Giampiero Cai, Gea Guerriero
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2019)
Review
Biochemistry & Molecular Biology
Stefano Del Duca, Iris Aloisi, Luigi Parrotta, Giampiero Cai
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Horticulture
Chiara Piccini, Luigi Parrotta, Claudia Faleri, Marco Romi, Stefano Del Duca, Giampiero Cai
SCIENTIA HORTICULTURAE
(2019)
Article
Plant Sciences
Roberto Berni, Marco Romi, Claudio Cantini, Jean-Francois Hausman, Gea Guerriero, Giampiero Cai
FRONTIERS IN PLANT SCIENCE
(2019)
Editorial Material
Biochemistry & Molecular Biology
Giampiero Cai, Stefano Del Duca
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Biochemistry & Molecular Biology
Roberto Berni, Mubasher Zahir Hoque, Sylvain Legay, Giampiero Cai, Khawar Sohail Siddiqui, Jean-Francois Hausman, Christelle M. Andre, Gea Guerriero
Article
Plant Sciences
Manuela Mandrone, Fabiana Antognoni, Iris Aloisi, Giulia Potente, Ferruccio Poli, Giampiero Cai, Claudia Faleri, Luigi Parrotta, Stefano Del Duca
FRONTIERS IN PLANT SCIENCE
(2019)
Article
Multidisciplinary Sciences
Roberto Berni, Emilie Piasecki, Sylvain Legay, Jean-Francois Hausman, Khawar Sohail Siddiqui, Giampiero Cai, Gea Guerriero
SCIENTIFIC REPORTS
(2019)
Article
Horticulture
Roberto Berni, Marco Romi, Luigi Parrotta, Giampiero Cai, Claudio Cantini