Article
Environmental Sciences
Tong Zhang, Xuming Zhuang, Shakeel Ahmad, Taeho Lee, Chengbo Cao, Shou-Qing Ni
Summary: This study investigated the role of dissimilatory nitrate reduction to ammonium (DNRA) in the stability of urban river networks and marine ecosystems. The research identified a significant positive correlation between DNRA rate and sediment organic carbon, with Anaeromyxobacter as the dominant genus and both heterotrophic and autotrophic DNRA species present. It added diversity to urban freshwater river ecosystem studies and provided new insights into the biological nitrogen cycle of typical urban inland rivers in eastern China.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Environmental Sciences
Hezhong Yuan, Bingchan Jia, Qingfei Zeng, Yanwen Zhou, Juan Wu, Haixiang Wang, Hao Fang, Yiwei Cai, Qiang Li
Summary: This study assessed the impact of dissimilatory nitrate reduction on the phosphorus cycle in lacustrine sediment. The results showed that nitrate reduction facilitated the accumulation of phosphorus in lake water. The synchronous control of nitrogen and phosphorus is crucial for the management and restoration of lake eutrophication.
Article
Environmental Sciences
Achen Wang, Xiang Li, Xiuli Hao, Xuesong Luo, Wenli Chen, Qiaoyun Huang
Summary: Heavy metal remediation treatments can influence the assembly of functional microbial communities, specifically the Dissimilatory nitrate reduction to ammonia (DNRA) bacteria. The abundance and diversity of the DNRA bacterial community were correlated with pH, nitrogen availability, and metal concentrations. The assembly processes were mainly driven by deterministic factors, with NH4+ playing a crucial role. However, pH, phosphorus, and metal availability also had auxiliary regulatory effects on the DNRA bacterial community.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Ecology
Yaohao Hu, Yiguo Hong, Jiaqi Ye, Jiapeng Wu, Yu Wang, Fei Ye, Xiangyang Chang, Aimin Long
Summary: The study revealed a diversity and richness variation of DNRA bacteria along a salinity gradient in sediment cores of the Pearl River Estuary, with specific spatial distribution of DNRA bacterial communities driven by environmental factors. Dominant DNRA bacteria were identified as key populations in the PRE sediments.
Article
Environmental Sciences
Xiaowen Li, Dong Bai, Qinghui Deng, Xiuyun Cao, Yiyong Zhou, Chunlei Song
Summary: DNRA rates in Xiamen Bay were influenced by sulfide, organic carbon, nrfA gene abundances, and DNRA bacteria community. The main phyla of DNRA bacteria were Bacteroidetes and Proteobacteria, with Pelobacter being the dominant genus. Additionally, the correlation analysis showed positive relationships between DNRA rate, nrfA abundance, environmental factors such as nitrite, total organic carbon, acid volatile sulfide, and total nitrogen.
JOURNAL OF SOILS AND SEDIMENTS
(2021)
Article
Biotechnology & Applied Microbiology
Megha Kaviraj, Upendra Kumar, A. K. Nayak, Soumendranath Chatterjee
Summary: This study conducted in-silico protein modeling of NrfA in three model DNRA bacteria, revealing that the majority of NrfA protein is located in the periplasm and is hydrophilic. Conserved motifs and amino acid sequences were identified, and a novel identical sequence between serine and proline was discovered. Modeller was found to be the best tool for predicting NrfA protein. These findings contribute to the identification of unexplored DNRA bacteria and shed light on the N-retention processes.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Environmental Sciences
Donlaporn Sripan, Alisa Wilantho, Khunnalack Khitmoh, Doonyapong Wongsawaeng, Jamal Ouazzani, Suchana Chavanich, Sissades Tongsima, Naraporn Somboonna
Summary: This study revealed microbiota and their metabolic potentials at an epipelagic depth in the southeast Andaman Sea of Thailand, showing significant differences between shallow and deep microbial communities.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Biochemical Research Methods
Paulo Roberto Queiroz, Marina Cassago Posso, Erica Soares Martins, Priscila Grynberg, Roberto Togawa, Rose Gomes Monnerat
Summary: Bacillus thuringiensis is an important bacterium in the Bacillus cereus group due to its insecticidal properties. The detection of cry genes is crucial for characterizing strains and indicating their toxic potential. A multiplex assay using specific primers was developed to identify the presence of cry1A, cry1C, and cry1F genes in samples of B. thuringiensis. The system was validated and shown to be reliable in characterizing strains.
JOURNAL OF MICROBIOLOGICAL METHODS
(2023)
Article
Engineering, Environmental
Junkai Gao, Guanglong Liu, Xiaowen Li, Mengjuan Tang, Xiuyun Cao, Yiyong Zhou, Chunlei Song
Summary: This study investigated the impact of organic carbon quantity and composition on nitrate reduction pathways in two basins of Lake Chaohu in Hefei City, China. It was found that the serious algal bloom in the west basin resulted in higher organic carbon accumulation and nitrate deficiency, leading to a high C/NO3--N ratio. This triggered dissimilatory nitrate reduction to ammonium (DNRA) and specific bacterial communities played a role in this process.
Article
Multidisciplinary Sciences
Eric A. Nalefski, Nidhi Patel, Philip J. Y. Leung, Zeba Islam, Remy M. Kooistra, Ishira Parikh, Estelle Marion, Gavin J. Knott, Jennifer A. Doudna, Anne-Laure M. Le Ny, Damian Madan
Summary: The Cas13a and Cas12a enzymes in bacterial CRISPR systems exhibit distinct activity characteristics, with Cas13a showing high-turnover trans-RNase activity and Cas12a showing low-turnover trans-nuclease activity.
Article
Environmental Sciences
Pengfei Qiu, Youxin Chen, Chenjie Li, Da Huo, Yonghong Bi, Jianbo Wang, Yunchuang Li, Renhui Li, Gongliang Yu
Summary: This study used NGS and qPCR techniques to reveal the diversity and dynamics of cyanobacteria and 2-MIB-producing cyanobacteria in Yuctiao Reservoir in Tianjin, China. NGS identified the cyanobacterial community composition, while qPCR detected the 2-MIB synthesis gene copy number, predicting the increase in 2-MIB concentration through gene copy number. The combination of NGS and qPCR can provide early warning information for reservoir managers and water utilities facing taste and odor incidents.
ENVIRONMENTAL POLLUTION
(2021)
Article
Plant Sciences
Pierre-Louis Alaux, Coralie Mison, Carolina Senes-Guerrero, Virginie Moreau, Gilles Manssens, Guy Foucart, Sylvie Cranenbrouck, Stephane Declerck
Summary: The study found that AMF diversity varied greatly among maize fields in South Belgium, with the number of species ranging from 1 to 37. There was a significant effect of management on AMF diversity, with organic management showing the highest diversity and richness values compared to conventional management or conversion.
Article
Engineering, Environmental
David Correa-Galeote, Alba Roibas-Rozas, Anuska Mosquera-Corral, Belen Juarez-Jimenez, Jesus Gonzalez-Lopez, Belen Rodelas
Summary: This study found that the microbial communities in industrial wastewater treatment plants treating fish-canning effluents were sensitive to NaCl concentration, and there were significant differences in microbial community structure among different plants.
JOURNAL OF WATER PROCESS ENGINEERING
(2021)
Article
Soil Science
Antonio Castellano-Hinojosa, Willm Martens-Habbena, Sarah L. Strauss
Summary: Tree crops can benefit from cover crops by improving soil nutrient availability and reducing greenhouse gas emissions. The composition of cover crops affects the abundance and diversity of nitrogen cycling communities in the soil, which ultimately impacts greenhouse gas emissions.
Article
Agriculture, Dairy & Animal Science
Edgardo Diaz-Ferguson, Magaly Chial, Maribel Gonzalez, Edgardo Munoz, Olga Chen, Ovidio Duran, Angel Javier Vega, Carlos Ramos Delgado
Summary: Molecular identification of fish tissue samples from 203 individuals was conducted based on cytochrome oxidase I gene segment sequencing. A total of 34 species from 14 families were identified, including three Caribbean species. Genetic diversity and connectivity between Gulf areas were compared, and a high level of connectivity was observed. The study also designed primers and dual-labeled probes for environmental DNA detection and qPCR detection.