Article
Multidisciplinary Sciences
Lauren C. Panzera, Ben Johnson, Josiah A. Quinn, In Ha Cho, Michael M. Tamkun, Michael B. Hoppa
Summary: Research indicates that Kv2.1 channels play a crucial role in facilitating ER calcium uptake during electrical activity in neurons, which is related to synaptic transmission. Loss of the Kv2.1 channel can disrupt synaptic vesicle fusion during stimulation, while its non-conducting role can link ER calcium uptake with electrical activity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Chungwon Park, Xingxiang Chen, Chong-Li Tian, Gyu Nam Park, Nicolas Chenouard, Hunki Lee, Xin Yi Yeo, Sangyong Jung, Richard W. Tsien, Guo-Qiang Bi, Hyokeun Park
Summary: Maintaining the balance between excitatory and inhibitory synaptic transmission is crucial for proper central nervous system function. Inhibitory synaptic vesicles exhibit unique dynamics and fusion properties that support rapid synaptic inhibition, showing differences compared to excitatory synaptic vesicles labeled with synaptotagmin-1. This study provides insights into the specialized mechanisms of inhibitory synaptic vesicle release, contributing to the understanding of coordinated excitation and inhibition in the CNS.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Mia Krout, Kelly H. Oh, Ame Xiong, Elisa B. Frankel, Peri T. Kurshan, Hongkyun Kim, Janet E. Richmond
Summary: Synaptic transmission requires the coordinated activity of multiple synaptic proteins at the active zone (AZ). CLA-1 and UNC-10 work together to regulate the levels of the UNC-2 calcium channel and the localization of UNC-13 at the synapse. These findings suggest a conserved arrangement of AZ scaffolding proteins that are crucial for precise coupling to Ca2+ channels.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biology
Chad Paul Grabner, Tobias Moser
Summary: The study demonstrates that the synaptic ribbon in rod photoreceptors facilitates calcium influx and establishes a large readily releasable pool of synaptic vesicles.
Article
Neurosciences
Michael J. Seibert, Chantell S. Evans, Kevin S. Stanley, Zhenyong Wu, Edwin R. Chapman
Summary: SYT9 is a Ca2+ sensor in neuroendocrine cells, but its function in neurons is unclear. In this study, it was found that SYT9 does not trigger rapid synaptic vesicle exocytosis in mouse cortical, hippocampal, or striatal neurons unless it is overexpressed. Loss of SYT9 in striatal neurons reduced the frequency of spontaneous neurotransmitter release events. Further investigation revealed that SYT9 is localized to dense-core vesicles containing substance P, and loss of SYT9 impaired SP release, causing the observed decrease in mini frequency. The study also showed that Ca2+ binding to the C2A domain of SYT9 triggered membrane fusion in vitro, and mutations disrupting this activity abolished SYT9's ability to regulate SP release and mini frequency. Therefore, it can be concluded that SYT9 indirectly regulates synaptic transmission in striatal neurons by controlling SP release.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Biology
Janus R. L. Kobbersmed, Manon M. M. Berns, Susanne Ditlevsen, Jakob B. Sorensen, Alexander M. Walter
Summary: Synaptic communication relies on the fusion of synaptic vesicles with the plasma membrane, which is achieved through the allosteric stabilization of synaptotagmin's Ca2+/PI(4,5)P-2 dual-bound state and the coordinated function of multiple synaptotagmins.
Article
Agriculture, Dairy & Animal Science
G. L. Voronin, G. Ning, J. N. Coupland, R. Roberts, F. M. Harte
Summary: High-pressure-jet processing can alter the physiochemical properties of low-fat ice cream by changing the density, particle size, rheological behavior, hardness, and melting rate. This treatment disrupts casein micelles and fat droplets, leading to unique microstructures and potentially enhanced properties of the ice cream.
JOURNAL OF DAIRY SCIENCE
(2021)
Review
Neurosciences
Jacopo Lamanna, Mattia Ferro, Sara Spadini, Antonio Malgaroli
Summary: This article reviews custom methods for evaluating and modifying synaptic activity, discussing their advantages, limitations, and applications in vitro and in vivo studies. The article also discusses challenges in extending these methods to the in vivo setting and the potential of using optogenetic approaches to manipulate synaptic function in the living brain.
EUROPEAN JOURNAL OF NEUROSCIENCE
(2022)
Article
Neurosciences
Soulmee Koh, Wongyoung Lee, Sang Myun Park, Sung Hyun Kim
Summary: The study demonstrates the important role of Cav1 in synaptic vesicle exocytosis, with its knockdown significantly impairing synaptic vesicle function. Neurons rescued by triple mutants lacking palmitoylation sites of Cav1 show impairments in both synaptic transmission and retrieval, highlighting the importance of Cav1 palmitoylation in activity-driven synaptic vesicle dynamics.
Article
Anesthesiology
Iris A. Speigel, Hugh C. Hemmings
Summary: The study reveals that isoflurane inhibits synaptic vesicle exocytosis from hippocampal glutamatergic neurones and GABAergic interneurones in a cell-type-specific manner depending on their expression of voltage-gated sodium channel subtypes.
BRITISH JOURNAL OF ANAESTHESIA
(2021)
Article
Neurosciences
Xiaofang Zhao, Yuan Guan, Fengwei Liu, Shuxin Yan, Yalong Wang, Meiqin Hu, Yuhong Li, Rena Li, Claire Xi Zhang
Summary: The cell-to-cell transmission of pathological alpha-synuclein is a critical event in synucleinopathies, and its secretion is shown to be SNARE-dependent and mediated by multiple vesicular pathways.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Neurosciences
Gean Domingos-Souza, Diana Martinez, Steven Sinkler, Cheryl M. Heesch, David D. Kline
Summary: The study investigated the effects of CIH on PVN neurons and found that a1-AR activation plays a role in neuronal responses after CIH, which are attenuated compared to normal conditions. These findings may shed light on alterations in the cardiovascular, respiratory, and autonomic nervous systems in obstructive sleep apnea.
EXPERIMENTAL NEUROLOGY
(2021)
Article
Physics, Multidisciplinary
Francesco Randi, Andrew M. Leifer
Summary: This paper investigates the time-dependent functional connectivity between neurons in the brain, using nonequilibrium Green's functions to describe the dynamic behavior of neuron networks. Through numerical calculations and examples inspired by Caenorhabditis elegans, the relationship between connectivity and response functions is demonstrated.
PHYSICAL REVIEW LETTERS
(2021)
Article
Biology
Abagael M. Lasseigne, Fabio A. Echeverry, Sundas Ijaz, Jennifer Carlisle Michel, E. Anne Martin, Audrey J. Marsh, Elisa Trujillo, Kurt C. Marsden, Alberto E. Pereda, Adam C. Miller
Summary: This study investigated the functional relationship between neuronal Connexins and ZO1, revealing that ZO1 is crucial for the localization of synaptic Connexins, while Connexins are not necessary for ZO1 localization. Disruption of this hierarchical ZO1/Connexin relationship abolishes electrical transmission and disrupts Mauthner cell-initiated escape responses.
Article
Multidisciplinary Sciences
Xiaomeng Wang, Xiaotong Wu, Hao Wu, Hanyang Xiao, Sijia Hao, Bingwei Wang, Chen Li, Katherin Bleymehl, Stefan G. Kauschke, Volker Mack, Boris Ferger, Holger Klein, Ruimao Zheng, Shumin Duan, Hao Wang
Summary: Research has discovered that long-term activation of GABAergic cells in vlPAG can rescue obesity induced by high-fat diet. This treatment method reduces food intake, increases energy expenditure, and promotes browning of adipose tissue. Overexpression of CACNA2D1 gene can reverse obesity and is considered a potential target for obesity treatment.
Article
Multidisciplinary Sciences
Dragomir Milovanovic, Yumei Wu, Xin Bian, Pietro De Camilli
Article
Cell Biology
Nah-Young Shin, Hyewon Choi, Lynn Neff, Yumei Wu, Hiroaki Saito, Shawn M. Ferguson, Pietro De Camilli, Roland Baron
JOURNAL OF CELL BIOLOGY
(2014)
Article
Medicine, Research & Experimental
Keita Soda, Daniel M. Balkin, Shawn M. Ferguson, Summer Paradise, Ira Milosevic, Silvia Giovedi, Laura Volpicelli-Daley, Xuefei Tian, Yumei Wu, Hong Ma, Sung Hyun Son, Rena Zheng, Gilbert Moeckel, Ottavio Cremona, Lawrence B. Holzman, Pietro De Camilli, Shuta Ishibe
JOURNAL OF CLINICAL INVESTIGATION
(2012)
Article
Cell Biology
Hongying Shen, Francesca Giordano, Yumei Wu, Jason Chan, Chen Zhu, Ira Milosevic, Xudong Wu, Kai Yao, Bo Chen, Tobias Baumgart, Derek Sieburth, Pietro De Camilli
NATURE CELL BIOLOGY
(2014)
Article
Multidisciplinary Sciences
Xuelin Lou, Fan Fan, Mirko Messa, Andrea Raimondi, Yumei Wu, Loren L. Looger, Shawn M. Ferguson, Pietro De Camilli
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2012)
Article
Multidisciplinary Sciences
Daehun Park, Yumei Wu, Sang-Eun Lee, Goeun Kim, Seonyoung Jeong, Dragomir Milovanovic, Pietro De Camilli, Sunghoe Chang
Summary: The combination of synapsin and synaptophysin proteins in fibroblasts efficiently reconstitutes synaptic vesicle clusters with similar morphology and liquid-like properties to those found at synapses, suggesting they play a key role in the assembly of synaptic structures.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Fan Fan, Yumei Wu, Manami Hara, Adam Rizk, Chen Ji, Dan Nerad, Natalia Tamarina, Xuelin Lou
Summary: This study revealed the crucial role of dynamins in endocytosis and secretion capacity of pancreatic beta cells, indicating that deficiency in dynamin-mediated endocytosis can lead to impaired insulin secretion, increasing the risk of endocrine dysfunction and diabetes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Shujun Cai, Yumei Wu, Andres Guillen-Samander, William Hancock-Cerutti, Jun Liu, Pietro De Camilli
Summary: Researchers have found in situ evidence for the bridge-like lipid transport mechanism of VPS13 at membrane contact sites using AlphaFold predictions and cryo-electron tomography.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Andres Guillen-Samander, Yumei Wu, S. Sebastian Pineda, Francisco J. Garcia, Julia N. Eisen, Marianna Leonzino, Berrak Ugur, Manolis Kellis, Myriam Heiman, Pietro De Camilli
Summary: Chorea-acanthocytosis and McLeod syndrome are diseases caused by mutations in VPS13A and XK genes, respectively, with common features of degenerated caudate neurons and abnormally shaped erythrocytes. VPS13A can be localized at ER-plasma membrane contacts by binding to the cytosolic loop of XK, suggesting its involvement in lipid transfer between the ER and the plasma membrane.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Hanieh Falahati, Yumei Wu, Vanessa Feuerer, Hans-Georg Simon, Pietro De Camilli
Summary: The spine apparatus, a specialized compartment of the neuronal smooth ER, is still poorly understood. The identification of proteins associated with the spine apparatus provides insights into its biogenesis and maintenance, as well as its role in physiological and pathological processes in dendritic spines.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Daehun Park, Yumei Wu, Xinbo Wang, Swetha Gowrishankar, Aaron Baublis, Pietro De Camilli
Summary: ATG9 is the sole transmembrane protein found at presynapses in the autophagy machinery. In this study, the authors demonstrate that synaptophysin and ATG9A vesicles form condensates with synapsin but remain separate from each other. Unlike synaptophysin, other integral SV membrane proteins do not form condensates with synapsin but assemble into the clusters formed by the synaptophysin and synapsin proteins. ATG9A localizes to a distinct class of vesicles that also interacts with synapsin but forms a different phase. These findings indicate differential sorting of ATG9A compared to SV proteins and highlight a dual role of synapsin in controlling clustering of SVs and ATG9A vesicles at synapses.
NATURE COMMUNICATIONS
(2023)