Article
Cell Biology
Stuart J. Mills, Parinaz Ahangar, Hannah M. Thomas, Benjamin R. Hofma, Rachael Z. Murray, Allison J. Cowin
Summary: TLR4 and Flii play crucial roles in inflammation and tissue repair. Elevated Flii reduces early TLR4 expression, impairing healing, while reduced Flii contributes to early healing.
Article
Biochemistry & Molecular Biology
Hai Zhong, Huiyi Liang, Yanzi Yan, Lei Chen, Tianyu Zhao, Lixin Liu, Yongming Chen
Summary: Chronic inflammation is a major obstacle in refractory diabetic foot ulcers, with high levels of cfDNA playing a crucial role in inflammation. Using cationic hydrogels can significantly reduce cfDNA levels, eliminate inflammation, promote wound closure, and does not introduce systemic toxicity.
Article
Biochemistry & Molecular Biology
Xiao-Hui Wang, Wei Guo, Wei Qiu, Luo-Quan Ao, Meng-Wei Yao, Wei Xing, Yang Yu, Quan Chen, Xiao-Feng Wu, Zhan Li, Xue-Ting Hu, Xiang Xu
Summary: This study reveals a novel function of PD-L1 in wound healing, showing that PD-L1 acts as a positive regulator by forming an immunosuppressive microenvironment, modulating macrophage polarization and promoting inflammation resolution. Loss of PD-L1 delays wound healing, especially in the presence of severe inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Review
Cell Biology
Alireza Hassanshahi, Mohammad Moradzad, Saman Ghalamkari, Moosa Fadaei, Allison J. Cowin, Mohammadhossein Hassanshahi
Summary: This review discusses the role and mechanisms of macrophages in skin wound healing, including cellular and molecular aspects such as subtypes, metabolism, plasticity, and epigenetics. The interactions between macrophages and factors important for wound healing are also highlighted.
Article
Pharmacology & Pharmacy
Mengfan Wu, Dany Y. Matar, Zhen Yu, Ziyu Chen, Samuel Knoedler, Brian Ng, Oliver A. Darwish, Sadaf Sohrabi, Leigh Friedman, Valentin Haug, George F. Murphy, Yuval Rinkevich, Dennis P. Orgill, Adriana C. Panayi
Summary: This study investigates the impact of negative pressure wound therapy (NPWT) on the pathophysiology of fibrosis. The results show that NPWT can reduce fibrosis-related protein factors, increase cell proliferation, and decrease cell apoptosis. NPWT uncouples the association between YAP and EPF activation and has promising potential in improving scar appearance.
Article
Chemistry, Medicinal
Jianying Song, Jing Zeng, Silin Zheng, Nan Jiang, Anguo Wu, Shengming Guo, Rupei Ye, Lixin Hu, Feihong Huang, Long Wang, Xiaogang Zhou, Bo Liu, Jianming Wu, Qi Chen
Summary: Sanguisorba officinalis L. ethanol extract (ESO) can accelerate the healing process of diabetic wounds (DW) by suppressing the inflammatory response and increasing the ratio of M2 to M1 polarization.
PHYTOTHERAPY RESEARCH
(2023)
Review
Pharmacology & Pharmacy
Kang Geng, Xiumei Ma, Zongzhe Jiang, Wei Huang, Chenlin Gao, Yueli Pu, Lifang Luo, Youhua Xu, Yong Xu
Summary: Recent studies have shown that the interaction between immune and metabolic responses is crucial for maintaining tissue and organ homeostasis, playing a significant role in the development of chronic diseases. In diabetic wound healing, the abnormal activation of innate immune cells is essential in triggering and sustaining inflammation. Targeting these innate immune pathways and reprogramming immune cell phenotypes show great potential in diabetic wound therapy.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Jing Liu, Moyuan Qu, Canran Wang, Yumeng Xue, Hui Huang, Qianming Chen, Wujin Sun, Xingwu Zhou, Guihua Xu, Xing Jiang
Summary: This study designed a hemostatic and anti-inflammatory hydrogel patch for promoting diabetic wound healing. The hydrogel was able to regulate macrophage phenotype, promote fibroblast proliferation and migration, and accelerate angiogenesis. The hydrogel can effectively promote wound closure and tissue regeneration, making it a simple, economical, effective, and safe treatment for diabetic wounds.
Article
Nanoscience & Nanotechnology
Kunbao Zhang, Chao Yang, Chuanxu Cheng, Chengxin Shi, Madi Sun, Hanze Hu, Tongfei Shi, Xuenian Chen, Xuan He, Xiao Zheng, Mingqiang Li, Dan Shao
Summary: A bioactive hydrogel is developed for efficient and safe bacteria-infected diabetic wound healing by targeting inflammation and infection. The hydrogel can capture danger molecules and deliver antibiotics, resulting in improved wound closure rate and anti-inflammatory and antibacterial effects.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Nanoscience & Nanotechnology
Kunbao Zhang, Chao Yang, Chuanxu Cheng, Chengxin Shi, Madi Sun, Hanze Hu, Tongfei Shi, Xuenian Chen, Xuan He, Xiao Zheng, Mingqiang Li, Dan Shao
Summary: This study introduces a bioactive hydrogel, named OCMC-Tob/PEI hydrogel, for the treatment of infected wounds in patients with chronic diabetes. The hydrogel achieves dual effects of anti-inflammation and anti-infection by scavenging danger molecules and releasing antibiotics.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Immunology
Junren Chen, Siqi Qin, Shengmeng Liu, Kexin Zhong, Yiqi Jing, Xuan Wu, Fu Peng, Dan Li, Cheng Peng
Summary: Chronic inflammation is involved in the progression of various chronic diseases, including obesity, diabetes mellitus, and its complications. Diabetic ulcer, characterized by chronic non-healing wounds, is a serious complication of diabetes that significantly impacts patients' quality of life and imposes high medical costs. Matrix metalloproteases (MMPs), which play a crucial role in the healing process, have been identified as potential biomarkers for the diagnosis of diabetic ulcer and therapeutic targets for its treatment. This review focuses on natural products derived from herbs, vegetables, and animals that target MMPs and have shown potential in treating diabetic ulcer.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Lifeng Zhang, Jiali Yang, Wencong Liu, Qiteng Ding, Shuwen Sun, Shuai Zhang, Ning Wang, Yue Wang, Siyu Xi, Chunyu Liu, Chuanbo Ding, Changtian Li
Summary: This study reported a hydrogel dressing (PCA) based on L-arginine conjugated chitosan (CA) and aldehyde functionalized polysaccharides of Phellinus igniarius (OPPI) for wound repair in diabetes. PCA showed good antioxidant, antibacterial, and safety properties, and effectively promoted wound healing by promoting epithelial formation, collagen deposition, and enhancing angiogenesis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biotechnology & Applied Microbiology
Wenyan Huang, Ping Xu, Xiaoxue Fu, Jiaxin Yang, Weihong Jing, Yucen Cai, Yingjuan Zhou, Rui Tao, Zhangyou Yang
Summary: In this study, a functional molecule-mediated copper ions co-assembled strategy was developed for the collaborative treatment of diabetic wounds. The results showed that this strategy was effective in inhibiting bacterial growth through photothermal therapy and relieving wound hypoxia and improving inflammatory accumulation by catalyzing the conversion of intracellular hydrogen peroxide to oxygen. Additionally, the slow release of copper ions promoted cellular proliferation, migration, and angiogenesis, further enhancing the healing of diabetic wounds.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Review
Cell Biology
Sonya J. Wolf, William J. Melvin, Katherine Gallagher
Summary: Non-healing wounds in Type 2 Diabetes (T2D) patients are a common cause of amputation in the US, with an associated 5-year mortality of nearly 50%. Research indicates that macrophage function is crucial for proper wound healing, but in T2D patients and mice, macrophages exhibit a persistent inflammatory state.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Zhipeng Ni, Haojie Yu, Li Wang, Yudi Huang, Hui Lu, Haiying Zhou, Qingxian Liu
Summary: This study successfully utilized hydrogels containing tannic acid and ROS-responsive components to inhibit bacterial infection and oxidative stress in wounds, accelerating wound healing.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Cell Biology
Hanif Haidari, Lucia Melguizo-Rodriguez, Allison J. Cowin, Zlatko Kopecki
Summary: Healing of cutaneous wounds is crucial for the restoration of skin integrity and function. However, chronic wound infections pose a challenge due to resistance to conventional therapies. Antimicrobial peptides (AMPs) have emerged as a promising strategy for the treatment of these infections. Despite their potential, the clinical use of AMPs is hindered by their susceptibility to various factors. This review focuses on the development of nanotechnological approaches to enhance AMP delivery and stability, aiming to provide effective treatment options for wound infections.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xanthe L. L. Strudwick, Jason D. D. Whittle, Allison J. J. Cowin, Louise E. E. Smith
Summary: Fundamental knowledge about cell-surface interactions can be used in the development of wound dressings and scaffolds that promote wound healing. Plasma-coating wound dressings with acid-functionalised monomers or amine functionalisation can enhance cell adhesion, proliferation, and migration. In an in vivo study on mice, dressings coated with allylamine showed increased wound healing rate and reduced wound area, while prolonged treatment with allylamine-functionalised dressings may impair healing. Acrylic acid functionalisation did not improve early wound healing, but promoted collagen deposition at a later stage. This suggests that plasma polymerisation can lead to the development of new dressings that enhance wound closure by directing cell behavior, but a timed approach may be necessary for their application.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Christopher T. Desire, R. Dario Arrua, Xanthe L. Strudwick, Zlatko Kopecki, Allison J. Cowin, Emily F. Hilder
Summary: Significant technical advancements have been made in the field of western blotting through the use of microfluidic technologies over the past two decades. This review provides a critical overview of these advancements, discussing the advantages and disadvantages of each approach. The development of commercially available systems, including those for single cell analysis, is highlighted. The review also covers recent developments such as automation algorithms, different materials/chemistries, projection electrophoresis, and triBlots, as well as the potential of these systems for point-of-care devices in healthcare.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Review
Physiology
Frederique Dufour-Gaume, Nadira Frescaline, Venetia Cardona, Nicolas J. J. Prat
Summary: Hemorrhage is the leading cause of death in severe trauma injuries. Damage-associated molecular patterns (DAMPs) are released into the intercellular environment when organs or tissues experience prolonged hypoxia. The endothelium plays a significant role in both the release and effects of DAMPs, contributing to immune-inflammatory dysregulation and tissue suffering. This review focuses on the pathophysiological roles of DAMPs and explores emerging therapeutic interventions for severe trauma, aiming to calm the inflammatory response triggered by traumatic hemorrhagic shock.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Endocrinology & Metabolism
Sicco Bus, Matilde Monteiro-Soares, Fran J. Game, Jaap van Netten, Jan Apelqvist, Robert Fitridge, Eric C. Senneville, Nicolaas Schaper
Summary: The International Working Group on the Diabetic Foot (IWGDF) has published updated guidelines in 2023 on the prevention and management of diabetes-related foot disease. These guidelines cover various aspects such as prevention, classification, offloading, peripheral artery disease, infection, wound healing interventions, and Charcot neuro-osteoarthropathy. Implementing these guidelines by healthcare providers, public health agencies, and policymakers is expected to improve the management of diabetes-related foot disease and reduce the burden caused by this condition worldwide.
DIABETES-METABOLISM RESEARCH AND REVIEWS
(2023)
Article
Endocrinology & Metabolism
Jaap J. A. van Netten, Sicco A. Bus, Jan Apelqvist, Pam Chen, Vivienne Chuter, Robert Fitridge, Frances J. Game, Robert J. A. Hinchliffe, Peter A. Lazzarini, Joseph Mills, Matilde Monteiro-Soares, Edgar J. G. M. Peters, Katherine M. Raspovic, Eric K. Senneville, Dane K. C. Wukich, Nicolaas C. Schaper
Summary: In order to facilitate clear communication between individuals with diabetes-related foot disease and professionals worldwide, the International Working Group on the Diabetic Foot (IWGDF) has developed a set of definitions and criteria for diabetes-related foot disease based on systematic reviews of the literature. This document describes the 2023 update of these definitions and criteria.
DIABETES-METABOLISM RESEARCH AND REVIEWS
(2023)
Article
Surgery
Christopher P. Twine, Stavros K. Kakkos, Victor Aboyans, Iris Baumgartner, Christian-Alexander Behrendt, Sergi Bellmunt-Montoya, Bernd Jilma, Joakim Nordanstig, Athanasios Saratzis, Jim A. Reekers, Petar Zlatanovic
EUROPEAN JOURNAL OF VASCULAR AND ENDOVASCULAR SURGERY
(2023)
Article
Pharmacology & Pharmacy
Aswathy Ravindran Girija, Xanthe Strudwick, Sivakumar Balasubramanian, Vivekanandan Palaninathan, Sakthikumar Dasappan Nair, Allison J. J. Cowin
Summary: Electrospun fibers, particularly poly(L-lactic acid) (PLLA) scaffolds, have shown promising results in regenerative medicine. In this study, the in vivo performance of PLLA scaffolds with modified topology and collagen biofunctionalization in mouse wounds was evaluated. The results indicated that collagen biofunctionalization, especially on smooth scaffolds, improved wound healing by enhancing cellular infiltration, matrix deposition, and re-epithelialization. However, the differences in performance between in vitro and in vivo studies highlighted the importance of preclinical testing.
Review
Infectious Diseases
Matipaishe Mashayamombe, Miguel Carda-Dieguez, Alex Mira, Robert Fitridge, Peter S. Zilm, Stephen P. Kidd
Summary: The ability of Staphylococcus aureus to colonise different niches across the human body is linked to its adaptable metabolic capability and ability to persist within specific tissues. The relapse of disease in S. aureus infections is due to the presence of suboptimal populations that persist even after the disease is cleared. The constant arousal of these populations is a key strategy for long-term infection and survival of S. aureus.
Article
Microbiology
Oceane Blaise, Clarisse Leseigneur, Thomas Cokelaer, Elena Capuzzo, Nadira Frescaline, Olivier Dussurget
Summary: In this study, we sequenced the complete genomes of two clinical isolates of Staphylococcus aureus, Xen31 and Xen36, which were genetically modified to express an optimized Photorhabdus luminescens luciferase operon. These bioluminescent strains, Xen31 and Xen36, are widely used for studying bacterial pathogenesis, drug discovery, and the development of novel therapies.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Multidisciplinary Sciences
Veronika Bandara, Jade Foeng, Batjargal Gundsambuu, Todd S. Norton, Silvana Napoli, Dylan J. Mcpeake, Timona S. Tyllis, Elaheh Rohani-Rad, Caitlin Abbott, Stuart J. Mills, Lih Y. Tan, Emma J. Thompson, Vasiliki M. Willet, Victoria J. Nikitaras, Jieren Zheng, Iain Comerford, Adam Johnson, Justin Coombs, Martin K. Oehler, Carmela Ricciardelli, Allison J. Cowin, Claudine S. Bonder, Michael Jensen, Timothy J. Sadlon, Shaun R. Mccoll, Simon C. Barry
Summary: Chimeric antigen receptor (CAR)-T cell immunotherapy is a promising approach for treating solid tumors. Researchers have identified nfP2X7 as a tumor-specific antigen that is widely expressed on cancer cells but undetectable on healthy cells, making it a suitable target for CAR-T cell therapy. In mouse models of breast and prostate cancer, CAR-T cells targeting nfP2X7 showed significant anti-tumor efficacy.
NATURE COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
S. J. Mills, G. T. Kirby, B. R. Hofma, L. E. Smith, P. Statham, B. Vaes, A. E. Ting, R. Short, A. J. Cowin
Summary: Stem cell therapies for chronic wounds have had limited success. However, researchers have developed a dressing that supports the growth and transfer of stem cells to skin and speeds up wound healing in diabetic mice. This study shows potential for advanced therapeutic approaches in the treatment of chronic diabetic wounds.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Emmeline P. Virgo, Hanif Haidari, Zo L. Shaw, Louisa Z. Y. Huang, Tahlia L. Kennewell, Luke Smith, Taimur Ahmed, Saffron J. Bryant, Gordon S. Howarth, Sumeet Walia, Allison J. Cowin, Aaron Elbourne, Zlatko Kopecki
Summary: The current treatment methods for cutaneous wound infections are largely ineffective due to antimicrobial resistance. Black Phosphorus nanoflakes (BPNFs), a potential alternative antibacterial material, demonstrate over 99% killing efficiency against wound pathogens under ambient conditions without toxicity to mammalian skin cells. In preclinical models, BPNFs significantly reduce infection and accelerate wound healing, indicating their potential role in treating infected cutaneous wounds.
ADVANCED THERAPEUTICS
(2023)
