Article
Immunology
Lucia Biscari, Cintia Daniela Kaufman, Cecilia Farre, Victoria Huhn, Maria Florencia Pacini, Camila Bulfoni Balbi, Karina Andrea Gomez, Ana Rosa Perez, Andres Alloatti
Summary: This article presents a vaccination strategy based on dendritic cells (DCs) activated with LPS to enhance CD8(+) T cell responses against specific pathogens. The study found that this immunization scheme provides protection and reduces parasite burden in female mice, but not in male mice.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Fatima Ferragut, Karen M. Cruz, Juan P. Gallardo, Marisa Fernandez, Yolanda Hernandez Vasquez, Karina A. Gomez
Summary: This study focuses on T-cell assays that define the parasite-specificity based on upregulation of TCR stimulation-induced surface markers. The results demonstrate the efficacy of activation-induced markers (AIM) assays in detecting T. cruzi-specific CD4(+) and CD8(+) T cells in chronic Chagas disease patients, using different combinations of surface markers. Additionally, this approach is also successful in identifying the phenotype of activated T lymphocytes based on the expression of CD45RA and CCR7.
Article
Immunology
Cristina Gonzalez-Lopez, Wen-Hsiang Chen, Andrea Alfaro-Chacon, Liliana E. Villanueva-Lizama, Miguel Rosado-Vallado, Maria Jesus Ramirez-Sierra, Christian F. Teh-Poot, Jeroen Pollet, Oluwatoyin Asojo, Kathryn M. Jones, Peter J. Hotez, Maria Elena Bottazzi, Julio Vladimir Cruz-Chan
Summary: A therapeutic vaccine containing multiple antigenic epitopes has been developed for Chagas disease, showing promising effects in enhancing cellular immune responses and reducing parasite levels. This study provides a potential strategy for the development of a vaccine candidate for Chagas disease.
Article
Immunology
Jose Mateus, Paola Nocua, Paola Lasso, Manuel Carlos Lopez, M. Carmen Thomas, Adriana Egui, Claudia Cuervo, John Mario Gonzalez, Concepcion J. Puerta, Adriana Cuellar
Summary: The study investigates the relationship between parasite control and immune T cell responses in Chagas disease by using distinct infection approaches in an animal model. Different infection outcomes were observed in single or mixed T. cruzi infection with different genotypes, leading to differential immune CD8(+) T cell responses. This suggests that the quality of the CD8(+) T cell response may dictate differences in infection outcomes during chronic T. cruzi infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Raquel de Souza Vieira, Marilda Savoia Nascimento, Isau Henrique Noronha, Jose Ronnie Carvalho Vasconcelos, Luiz Alberto Benvenuti, Glen N. Barber, Niels Olsen Saraiva Camara, Jorge Kalil, Edecio Cunha-Neto, Rafael Ribeiro Almeida
Summary: STING signaling pathway plays an important role in immune response to Trypanosoma cruzi infection, and its absence negatively affects the activation of IRF-dependent pathways and cytokine expression.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Parasitology
Davi Alvarenga Lima, Leilane Oliveira Goncalves, Joao Luis Reis-Cunha, Paul Anderson Souza Guimaraes, Jeronimo Conceicao Ruiz, Daniel Barbosa Liarte, Silvane Maria Fonseca Murta
Summary: This study performed comparative transcriptomic analysis of wildtype and benznidazole-resistant T. cruzi populations, revealing metabolic pathways related to clinical drug resistance and identifying promising molecular targets for the development of new drugs for Chagas disease.
PARASITES & VECTORS
(2023)
Article
Immunology
Rosa Isela Galvez, Thomas Jacobs
Summary: Infection with Trypanosoma cruzi is the most neglected zoonosis in Latin America. CD8(+) T cells play a crucial role in controlling the infection, but cannot eliminate all parasites. Chronic infection leads to dysfunctional CD8(+) T cells with high expression of inhibitory receptor PD-1 and co-expression of transcription factor TOX.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Barbara Ferri Moraschi, Isau Henrique Noronha, Camila Pontes Ferreira, Leonardo M. Cariste, Caroline B. Monteiro, Priscila Denapoli, Talita Vrechi, Gustavo J. S. Pereira, Ricardo T. Gazzinelli, Joseli Lannes-Vieira, Mauricio M. Rodrigues, Karina R. Bortoluci, Jose Ronnie C. Vasconcelos
Summary: Deficiency in memory formation and increased immunosenescence are key features of Trypanosoma cruzi infection. A vaccination strategy using a DNA prime and recombinant adenovirus boost has shown efficacy in generating CD8(+) T-cell response against the parasite. Inhibiting mTOR with rapamycin during vaccination enhances CD8(+) T-cell response and improves survival rates in T. cruzi-infected mice, suggesting a potential use of mTOR inhibitor as an adjuvant in vaccine development against intracellular parasites.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Livia Silva Araujo Passos, Carolina Cattoni Koh, Luisa Mourao Dias Magalhaes, Maria do Carmo Pereira Nunes, Kenneth John Gollob, Walderez Ornelas Dutra
Summary: DN T cells play a critical role in the cytokine network associated with Chagas heart disease, with IND patients displaying more central memory cells and CARD patients having more effector memory cells. Central memory DN T cells in IND patients show a balanced cytokine profile, while effector memory DN T cells in CARD patients do not. Targeting the activation of effector memory DN T cells may be a potential strategy to control inflammation in Chagas cardiomyopathy and other inflammatory diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Concepcion J. Puerta, Adriana Cuellar, Paola Lasso, Jose Mateus, John M. Gonzalez
Summary: Trypanosoma cruzi, the causal agent of Chagas disease, has developed mechanisms of antigenic variability to evade the host immune response. CD8(+) T cells play a key role in chronic Chagas cardiomyopathy, and specific peptide stimulation can activate multifunctional immune responses. Anti-parasitic treatment improves CD8(+) T cell response, and the quality of CD8(+) T cell responses correlates with the outcome of chronic infection. These findings provide valuable resources for discovering new biomarkers, vaccines, and immunotherapy strategies.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Jose L. Saenz-Garcia, Beatriz S. Borges, Normanda Souza-Melo, Luiz V. Machado, Juliana S. Miranda, Lisandro Alfonso Pacheco-Lugo, Nilmar S. Moretti, Richard Wheleer, Lia C. Soares Medeiros, Wanderson D. DaRocha
Summary: The flagellum of Trypanosomatids contributes to multiple functions, and this study explores the role of Trypanin in T. cruzi. The deletion of Trypanin affects the growth and motility of T. cruzi epimastigotes, as well as their infection capacity.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Mukul Prasad, Lukasz Wojciech, Joanna Brzostek, Jianfang Hu, Yen Leong Chua, Desmond Wai Hon Tung, Jiawei Yap, Vasily Rybakin, Nicholas R. J. Gascoigne
Summary: Deletion of the Themis gene affects T cell selection in the thymus and TCR repertoire, leading to an increased proportion of V alpha 3.2 expressing cells in the CD8(+) T cell population. V alpha 3.2(+) cells exhibit a virtual memory phenotype, stronger response to self-pMHC, and bystander effector capability in Listeria infection. This reflects altered thymic selection and identifies a subset of cells present in wild-type mice.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Natalia Lins da Silva-Gomes, Leonardo Alexandre de Souza Ruivo, Claudia Moreira, Marcelo Meuser-Batista, Cristiane Franca da Silva, Denise da Gama Jaen Batista, Stenio Fragoso, Gabriel Melo de Oliveira, Maria de Nazare Correia Soeiro, Otacilio C. Moreira
Summary: In this study, genetically modified strains of Trypanosoma cruzi were used to evaluate the role of NTPDases in parasite infectivity. The results showed that parasites overexpressing TcNTPDase-1 had higher infectivity, while hemi-knockout parasites had lower infectivity and no significant electrocardiographic changes. These findings highlight the potential of NTPDases as a therapeutic target for Chagas disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Ana Carolina Leao, Laila Almeida Viana, Fernanda Fortes de Araujo, Rodrigo de Lourdes Almeida, Leandro Martins Freitas, Anderson Coqueiro-dos-Santos, Denise da Silveira-Lemos, Mariana Santos Cardoso, Joao Luis Reis-Cunha, Andrea Teixeira-Carvalho, Daniella C. Bartholomeu
Summary: Trypanosoma cruzi, the etiological agent of Chagas disease, exhibits high genetic and phenotypic diversity. The MASP family, located on the surface of T. cruzi, is involved in host-parasite interaction. Recombinant MASP proteins were used to investigate their immunogenicity in mice infected with different strains of T. cruzi. Flow cytometry analysis showed changes in MASP recognition profile during infection.
MICROBES AND INFECTION
(2022)
Article
Biochemistry & Molecular Biology
Fanny Mathias, Youssef Kabri, Damien Brun, Nicolas Primas, Carole Di Giorgio, Patrice Vanelle
Summary: Chagas disease caused by T. cruzi infection is a major public health problem in Latin America, resulting in 7000 annual deaths. This study synthesized ten new 1-substituted 2-nitropyrrole compounds and evaluated their biological activity. The derivatives showed good selectivity and activity against T. cruzi, but the maximum effect was lower than the reference drug.
Article
Cell Biology
Shweta Thakur, Monisha Dhiman, Anil K. Mantha
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Ramit Singla, Kunj Bihari Gupta, Shishir Upadhyay, Monisha Dhiman, Vikas Jaitak
BIOORGANIC & MEDICINAL CHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Ramit Singla, Kunal Prakash, Kunj Bihari Gupta, Shishir Upadhyay, Monisha Dhiman, Vikas Jaitak
BIOORGANIC CHEMISTRY
(2018)
Article
Oncology
Raman Preet Kaur, Rubal, Raja Paramjeet Singh Banipal, Rajesh Vashistha, Monisha Dhiman, Anjana Munshi
CURRENT PROBLEMS IN CANCER
(2019)
Article
Chemistry, Medicinal
Ramit Singla, Kunj Bihari Gupta, Shishir Upadhyay, Monisha Dhiman, Vikas Jaitak
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Shishir Upadhyay, Nidhi Sharma, Kunj Bihari Gupta, Monisha Dhiman
JOURNAL OF CELLULAR BIOCHEMISTRY
(2018)
Article
Food Science & Technology
Kunj Bihari Gupta, Shishir Upadhyay, Ram Gopal Saini, Anil K. Mantha, Monisha Dhiman
JOURNAL OF CEREAL SCIENCE
(2018)
Article
Endocrinology & Metabolism
Ravi P. Cholia, Monisha Dhiman, Raj Kumar, Anil K. Mantha
METABOLIC BRAIN DISEASE
(2018)
Article
Cell Biology
Shishir Upadhyay, Saurabh Vaish, Monisha Dhiman
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2019)
Article
Plant Sciences
Shishir Upadhyay, Anil Kumar Mantha, Monisha Dhiman
JOURNAL OF ETHNOPHARMACOLOGY
(2020)
Article
Environmental Sciences
Mandeep Kaur, Kunj Bihari Gupta, Shweta Thakur, Sukhchain Kaur, Monisha Dhiman
ENVIRONMENTAL TOXICOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Shweta Thakur, Bibekananda Sarkar, Monisha Dhiman, Anil K. Mantha
Summary: This study found that exposure to low concentrations of MCP and CP can lead to oxidative stress, DNA damage, modulate apoptosis, and APE1-mediated Nrf2 activation, which may be a potential mechanism promoting survival of lung cancer cells.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2021)
Article
Cell Biology
Kunj Bihari Gupta, Anil K. Mantha, Monisha Dhiman
Summary: The study evaluated the inflammatory effects of wheat protein gliadin and the protective role of curcumin on human intestinal cell lines. The results demonstrated that gliadin exacerbates inflammation and cellular damage, while curcumin attenuates the inflammation and helps maintain cellular morphology.
Review
Endocrinology & Metabolism
Sharanjot Kaur, Harkomal Verma, Sukhchain Kaur, Prabhakar Gangwar, Anuradha Yadav, Bharti Yadav, Rashmi Rao, Monisha Dhiman, Anil Kumar Mantha
Summary: miRNAs regulate gene expression and have multiple roles in biological processes and human diseases. In Alzheimer's disease (AD), miRNA dysregulation is associated with the clearance of amyloid beta and tau and the regulation of neuroinflammation. Several miRNAs related to AD are associated with cell cycle regulation, immune system, stress responses, cellular senescence, nerve growth factor signaling, and synaptic regulation. Phytochemicals can alter the expression of miRNAs and may serve as therapeutic agents for AD.
METABOLIC BRAIN DISEASE
(2023)
Review
Biochemistry & Molecular Biology
Shishir Upadhyay, Kunj Bihari Gupta, Anil Kumar Mantha, Monisha Dhiman
Summary: Doxorubicin (DOX) is beneficial for cancer patients, but its toxic effects on the heart can lead to cardiac dysfunction. Oxidative stress induced by DOX disrupts cardiac contractile muscle proteins, affecting the heart's rhythmic mechanism and oxygen consumption rate. This damage to contractile components and associated factors ultimately results in left ventricular ejection and dilation, highlighting the need for clinical markers and medications to address DOX-induced cardiotoxicity.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)