Article
Developmental Biology
Noriyuki Satoh, Kanako Hisata, Stephany Foster, Shumpei Morita, Koki Nishitsuji, Nathalie Oulhen, Hitoshi Tominaga, Gary M. Wessel
Summary: This study, using scRNA-seq technology, identified the significant roles of Brachyury-expressing cells in the body-plan formation of early sea urchin embryos. The results suggested that Brachyury is involved in the invagination process to form the stomodeum, and it is expressed in certain clusters of oral ectoderm cells.
DEVELOPMENTAL BIOLOGY
(2022)
Article
Developmental Biology
Abdull J. Massri, Laura Greenstreet, Anton Afanassiev, Alejandro Berrio, Gregory A. Wray, Geoffrey Schiebinger, David R. McClay
Summary: By using scRNA-seq and computational methods, researchers studied the transcriptional changes in cell states of sea urchin embryos from development to larval stage. They found that skeletogenic and primordial germ cell trajectories diverged early, and ectodermal progenitors were distinct from other lineages by the 6th cleavage. Endomesoderm cells originated at the 6th cleavage and diverged into endoderm and mesoderm fates asynchronously.
Article
Environmental Sciences
J. I. Bertucci, A. Juez, J. Bellas
Summary: The combined effects of ocean acidification and microplastic pollution on the early development of Paracentrotus lividus were studied. The results showed that both ocean acidification and microplastics can alter the growth and morphology of the embryo, with observed differences in morphology related to decreased width of larvae. The changes in larvae shape could affect their buoyancy and ability to obtain and ingest food.
Article
Cell Biology
Maria Isabella Negretti, Nina Boese, Natalia Petri, Stanislav Kremnyov, Nikoloz Tsikolia
Summary: The development of visceral left-right asymmetry in bilateria relies on initial symmetry breaking and subsequent molecular patterning. The expression of the transcription factor pitx2 on the left side is facilitated by the asymmetric expression of the nodal morphogen in the left lateral plate mesoderm. The mechanisms leading to the asymmetric nodal domain differ depending on the mode of symmetry breaking.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Jennifer Kreis, Celine Marie Camuto, Carolin Charlotte Elsner, Sebastian Vogel, Philipp Vick
Summary: Gastrulation is a crucial developmental process that involves tissue rearrangements and patterning events to shape the emerging vertebrate organism. This study reveals the involvement of Rab7 in dorsal mesoderm patterning, which is crucial for FGF-dependent mesoderm specification and left-right asymmetry.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Natalia Abramenko, Marina Semenova, Alexander Khina, Pavel Zherebin, Yurii Krutyakov, Evgeny Krysanov, Leonid Kustov
Summary: Surface modification of nanoparticles with different stabilizers is commonly used to enhance their stability and applicability. Silver nanoparticle dispersions with biologically active stabilizers show potential as plant protection products, improving crop growth, yield, and protection. However, these stabilizers exhibit toxic effects on sea urchin embryos, with silver ions being more toxic than the nanoparticles themselves.
Review
Cell Biology
Kadeen Forrest, Alexandria C. Barricella, Sonny A. Pohar, Anna Maria Hinman, Jeffrey D. Amack
Summary: Human laterality disorders are caused by embryonic developmental issues. Studying the development of LRO (left-right organizer) in zebrafish models helps us understand the origins of laterality disorders.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Environmental Sciences
Laura DeMiguel-Jimenez, Nestor Etxebarria, Helena C. Reinardy, Xabier Lekube, Ionan Marigomez, Urtzi Izagirre
Summary: The study utilized sea-urchin embryos as test-organisms and applied a multi-index approach to investigate the toxic effects of different oils in the environment. The findings suggest that under ice weathering resulted in lowered waterborne PAHs and genotoxicity but augmented embryo toxicity.
MARINE POLLUTION BULLETIN
(2022)
Article
Environmental Sciences
Laura DeMiguel-Jimenez, Dennis Bilbao, Ailette Prieto, Helena C. Reinardy, Xabier Lekube, Urtzi Izagirre, Ionan Marigomez
Summary: This investigation examines the influence of temperature on oil toxicity, both alone and in combination with dispersants (D). The toxicity of low-energy water accommodated fractions (LEWAF) of three oils (NNA crude oil, marine gas oil -MGO-, and IFO 180 fuel oil) produced at temperatures ranging from 5 to 25 degrees Celsius was assessed by measuring larval lengthening, abnormalities, developmental disruption, and genotoxicity in sea urchin embryos. The levels of polycyclic aromatic hydrocarbons (PAHs) were similar among LEWAFs but the PAH profiles varied with the type of oil and production temperature. The presence of dispersants increased genotoxicity, which varied depending on the LEWAF production temperature of each oil. Impaired lengthening, abnormalities, and developmental disruption were observed, with the severity of these effects depending on the type of oil, dispersant application, and LEWAF production temperature. Toxicity, partly associated with individual PAHs, was higher at lower LEWAF production temperatures.
MARINE POLLUTION BULLETIN
(2023)
Article
Toxicology
Michael D. Collins, Elvis Han Cui, Seung Won Hyun, Weng Kee Wong
Summary: The primary aim of this paper is to propose a more quantitative approach to design dose-response experiments, specifically concentration-response experiments. By using a model-based optimal design, more accurate statistical inference can be obtained for the underlying parameters of interest. The study demonstrates the efficiency gains of using a carefully selected model-based optimal design compared to an ad-hoc empirical design.
ARCHIVES OF TOXICOLOGY
(2022)
Article
Multidisciplinary Sciences
Lydia Djenoune, Mohammed Mahamdeh, Thai V. Truong, Christopher T. Nguyen, Scott E. Fraser, Martina Brueckner, Jonathon Howard, Shiaulou Yuan
Summary: The breaking of bilateral symmetry in most vertebrates relies on the motile cilia of the embryonic left-right organizer (LRO), which generate fluid flow. This study demonstrated that the immotile LRO cilia serve as mechanosensors for shear force in zebrafish. Mechanical force applied to immotile LRO cilia activated intraciliary calcium transients, requiring the cation channel Polycystin-2. The study also showed that mechanical force applied to LRO cilia could rescue and reverse cardiac situs in zebrafish lacking motile cilia.
Article
Anatomy & Morphology
Victor D. Vacquier, Amro Hamdoun
Summary: The study introduces new techniques for generating parthenogenetic larvae of the Southern California sea urchin species, Lytechinus pictus. The researchers present a gallery of photomicrographs of morphologically abnormal larvae for transcriptomic analysis, aiming to compare gene expression control between parthenogenotes and fertilization-derived larvae for insights into gene regulation mechanisms in this model organism. Knowledge gained from transcriptomics of sea urchin parthenogenotes could potentially contribute to parthenogenetic studies in mammalian embryos.
DEVELOPMENTAL DYNAMICS
(2021)
Article
Environmental Sciences
Chiara Gambardella, Francesca Marcellini, Carla Falugi, Stefano Varrella, Cinzia Corinaldesi
Summary: Morphological anomalies in sea urchin early development stages are used as biomarkers in studies on environmental impact. These anomalies are influenced by the developmental stage of exposure, stress intensity, and mechanisms affected by environmental agents. Classification and analysis of these anomalies can help assess the impact of stressors on marine organisms' growth and development, and advance monitoring of coastal marine ecosystems.
ENVIRONMENTAL POLLUTION
(2021)
Article
Environmental Sciences
Juan Bellas, Diego Rial, Juliana Valdes, Leticia Vidal-Linan, Juan Bertucci, Soledad Muniategui, Victor M. Leon, Juan A. Campillo
Summary: This study investigates the effects of three organic pollutants (chlorpyrifos, triphenyl phosphate, bisphenol A) on sea urchin larvae. The results show that chlorpyrifos has the highest toxicity, attributed to oxidative stress, modulation of acetylcholinesterase response, and reduced detoxification efficacy. The study also identifies glutathione reductase activity as a reliable biomarker of exposure for sea urchin early-life stages.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Cell Biology
Natalia A. Shylo, Sarah E. Smith, Andrew J. Price, Fengli Guo, Melainia McClain, Paul A. Trainor
Summary: Using live imaging, veiled chameleon embryos were observed to undergo asymmetric morphological changes that trigger asymmetric expression of Nodal cascade in the left lateral plate mesoderm. This makes veiled chameleons a new and unique model for studying the evolution of L-R patterning.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Developmental Biology
Jacob F. Warner, Vincent Guerlais, Aldine R. Amiel, Hereroa Johnston, Karine Nedoncelle, Eric Rottinger
Article
Acoustics
L. Allach El Khattabi, S. Brun, P. Gueguen, N. Chatron, E. Guichoux, S. Schutz, J. Nectoux, A. Sorlin, M. Quere, J. Boudjarane, V. Tsatsaris, L. Mandelbrot, C. Schluth-Bolard, J. M. Dupont, C. Rooryck, Alexandre Cormier, Claude Ferec, Juliana Da Foncesca Pipoli, Franck Letourneur, Celine Bonnet, Philippe Jonveaux, Claire Bardel, Arnaud Lagarde, Catherine Badens, Nicolas Levy, Veronique Duboc, Veronique Paquis-Fluckinger, Amandine Boureau-Wirth, Sylvie Bannwarth, Benoit Arveiler, Didier Lacombe, Genetique Medicale, Michel Goossens, Michel Vidaud, Jerome Massardier, Anne-Helene Saliou, Damien Sanlaville
ULTRASOUND IN OBSTETRICS & GYNECOLOGY
(2019)
Article
Genetics & Heredity
M. Dolores Molina, Magali Quirin, Emmanuel Haillot, Noeamie De Croze, Ryan Range, Mathieu Rouel, Felipe Jimenez, Radja Amrouche, Aline Chessel, Thierry Lepage
Article
Evolutionary Biology
Eve Gazave, Eric Rottinger
Summary: The ability of regeneration is common among animals, with different species showing varying levels of regenerative potential. Despite recent advances in regenerative studies, there is still a lack of comprehensive understanding of the evolution of animal regeneration.
JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION
(2021)
Article
Biology
Ingrid Lekk, Veronique Duboc, Ana Faro, Stephanos Nicolaou, Patrick Blader, Stephen W. Wilson
Article
Developmental Biology
Swann Floc'hlay, Maria Dolores Molina, Celine Hernandez, Emmanuel Haillot, Morgane Thomas-Chollier, Thierry Lepage, Denis Thieffry
Summary: During sea urchin development, the interplay between Nodal and BMP2/4 pathways through regulatory circuits determines the specification of ventral and dorsal territories. Using recombinant protein treatments and computational modeling, the study accurately simulated gene expression in wild-type embryos and various morphant phenotypes, highlighting the crucial impact of Smad activation rate in dorsal-ventral patterning. These results emphasize the key role of mutual antagonism between Nodal and BMP2/4 pathways in driving early dorsal-ventral patterning of the sea urchin embryo.
Article
Biochemistry & Molecular Biology
Barbara Porro, Thamilla Zamoum, Cedric Mallien, Benjamin C. C. Hume, Christian R. Voolstra, Eric Rottinger, Paola Furla, Didier Forcioli
Summary: This study investigated the flexibility of symbiont acquisition in the Mediterranean snakelocks sea anemone Anemonia viridis, revealing that the composition of within-host-symbiont populations is more dependent on geographical origin of the hosts rather than lineage. The similarities in host-symbiont communities were greater among different genets, suggesting a capacity for horizontal acquisition. The mixed-mode transmission strategy in A. viridis may explain the large phenotypic plasticity observed in this anemone.
Article
Cell Biology
Clara Fricano, Eric Roettinger, Paola Furla, Stephanie Barnay-Verdier
Article
Biochemical Research Methods
Veronique Duboc, David Pratella, Marco Milanesio, John Boudjarane, Stephane Descombes, Veronique Paquis-Flucklinger, Silvia Bottini
Summary: NIPT involves analyzing fetal DNA from maternal blood, but currently lacks an integrated data analysis pipeline and guidelines for reliable implementation in clinical settings.
BRIEFINGS IN BIOINFORMATICS
(2022)
Article
Developmental Biology
Veronique Duboc, Fatima A. Sulaiman, Eleanor Feneck, Anna Kucharska, Donald Bell, Muriel Holder-Espinasse, Malcolm P. O. Logan
Summary: This study dissects a gene regulatory network involving Tbx4, Pitx1, and Isl1 in the establishment of the hindlimb bud, revealing differences in pathways between hindlimb and forelimb formation. Live cell imaging of murine limb mesenchyme cells undergoing chondrogenesis shows changes in cellular behaviors required for differentiation of chondrogenic precursors. Proximal hindlimb defects in Tbx4 mutant mice are attributed to a failure in the early differentiation of chondroprogenitors into chondrocytes, explaining the origins of proximally biased limb defects.
Review
Cell Biology
Eric Roettinger
Summary: Scientists have been intrigued by the ability of metazoans to regenerate lost or injured body parts, with whole body regeneration being a widespread phenomenon in aquatic/marine invertebrates. The sea anemone Nematostella vectensis has emerged as a key research model for understanding the cellular and molecular mechanisms underlying whole-body regeneration. Comparing embryonic development and regeneration in the same organism helps provide insight into specific elements of regeneration.
Article
Biochemistry & Molecular Biology
David Pratella, Veronique Duboc, Marco Milanesio, John Boudjarane, Stephane Descombes, Veronique Paquis-Flucklinger, Silvia Bottini
Summary: Two tools have been developed to improve the reliability of non-invasive prenatal testing (NIPT) and facilitate standardization of the test results. The interrelationships between different parameters have been analyzed, revealing their profound connection.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Multidisciplinary Sciences
Aline Chessel, Noemie De Croze, Maria Dolores Molina, Laura Taberner, Philippe Dru, Luc Martin, Thierry Lepage
Summary: During early development of the sea urchin embryo, ERK signalling is activated in mesodermal precursors through a cell-autonomous mechanism involving the transcriptional activation of the gene encoding KSR3. KSR3 is an allosteric activator of RAF and can activate ERK signalling independently of RAS in non-chordate metazoa. The findings provide insights into the evolution and co-option of the ERK signalling pathway.
NATURE COMMUNICATIONS
(2023)
