Article
Biology
Jennifer E. Jones, Valerie Le Sage, Gabriella H. Padovani, Michael Calderon, Erik S. Wright, Seema S. Lakdawala
Summary: Influenza viruses evolve rapidly during infection through mutations or reassortment, potentially leading to strains with increased resistance or infectivity. Understanding how genome segments interact during reassortment can help predict future events and emergence of new pandemic strains. Research in this area could provide valuable insights for risk assessment and pandemic preparedness.
Article
Virology
Luoluo Wang, Lieven Van Meulebroek, Lynn Vanhaecke, Guy Smagghe, Ivan Meeus
Summary: Advanced virus detection technology has improved significantly, but there is a lack of technology to evaluate the impacts of viruses on bee physiology and health. A study evaluated the potential of hemolymph metabolites as biomarkers for identifying viral infection status in bees. The research successfully constructed a predictive model and identified potential biomarkers to differentiate virus infection from non-infection in bees.
Article
Virology
Shan-Ying Wu, Yu-Lun Chen, Ying-Ray Lee, Chiou-Feng Lin, Sheng-Hui Lan, Kai-Ying Lan, Man-Ling Chu, Pei-Wen Lin, Zong-Lin Yang, Yen-Hsu Chen, Wen-Hung Wang, Hsiao-Sheng Liu
Summary: This study reveals that DENV2 induces autophagy in lung and liver cancer cells, resulting in the formation of double-membrane autophagosomes containing viral proteins and full-length RNAs that are infectious. Additionally, DENV2 promotes HMGB1 exocytosis through secretory autophagy.
Article
Multidisciplinary Sciences
Niels Piot, Oliver Schweiger, Ivan Meeus, Orlando Yanez, Lars Straub, Laura Villamar-Bouza, Pilar De la Rua, Laura Jara, Carlos Ruiz, Martin Malmstrom, Sandra Mustafa, Anders Nielsen, Marika Mand, Reet Karise, Ivana Tlak-Gajger, Erkay Ozgor, Nevin Keskin, Virginie Dievart, Anne Dalmon, Anna Gajda, Peter Neumann, Guy Smagghe, Peter Graystock, Rita Radzeviciute, Robert J. Paxton, Joachim R. de Miranda
Summary: The study reveals that both biotic and abiotic factors, including managed host species, climatic variables, and beekeeping practices, influence the prevalence of viruses in wild bee populations.
SCIENTIFIC REPORTS
(2022)
Article
Entomology
Alexander J. McMenamin, Fenali Parekh, Verena Lawrence, Michelle L. Flenniken
Summary: The study demonstrated virus-specific responses at the cellular level in honey bees, with potential antiviral mechanisms identified. Further research is needed to fully understand honey bee antiviral defenses. Cultured primary honey bee cells show promise in addressing this knowledge gap in future studies involving multiple viruses.
Article
Virology
Mathew P. Kirby, Ciara Stevenson, Liam J. Worrall, Yihang Chen, Christina Young, Jisoo Youm, Natalie C. J. Strynadka, Douglas W. Allan, Eric Jan
Summary: Viruses rely on internal ribosome entry sites to utilize the host cell machinery for viral protein expression. Mutations in the hinge region of dicistrovirus IRES revealed its significant role in viral translation.
JOURNAL OF VIROLOGY
(2022)
Article
Immunology
Ijeoma Nwagbo, Adelaide Milani, Annalisa Salviato, Gianpiero Zamperin, Lanre Sulaiman, Nanven Maurice, Clement Meseko, Alice Fusaro, Ismaila Shittu
Summary: Infectious bursal disease (IBD) is a worldwide viral poultry disease that has significant economic and food security impacts. This study focuses on the dynamics of infectious bursal disease virus (IBDV) evolution in Nigeria. The results show the presence of conserved markers associated with highly virulent IBDV strains, as well as unique amino acid mutations with unknown biological functions. Reassortant IBDVs were found circulating in Nigeria, which may explain the failure of vaccination in the poultry population.
Article
Environmental Sciences
Yanchun Deng, Sa Yang, Hongxia Zhao, Ji Luo, Wenchao Yang, Chunsheng Hou
Summary: Antibiotics are widely used and present in the environment, causing negative impacts on organisms. This study investigated the increased sensitivity of honey bees to viral infection due to antibiotic exposure. The findings showed that antibiotic treatment altered the gut microbiome in bees and promoted their susceptibility to viral infection.
ENVIRONMENTAL POLLUTION
(2022)
Article
Virology
Katie F. Daughenbaugh, Idan Kahnonitch, Charles C. Carey, Alexander J. McMenamin, Tanner Wiegand, Tal Erez, Naama Arkin, Brian Ross, Blake Wiedenheft, Asaf Sadeh, Nor Chejanovsky, Yael Mandelik, Michelle L. Flenniken
Summary: This study found viral sharing of four common viruses between honey bees and mining bees, as well as identification of a unique Tombo-like virus called Andrena-associated bee virus-1 (AnBV-1). Statistical modeling revealed that ecological factors such as floral diversity and abundance significantly impact the transmission of AnBV-1 in different habitats.
Article
Biochemistry & Molecular Biology
Barbara Szutkowska, Klaudia Wieczorek, Ryszard Kierzek, Pawel Zmora, Jake M. Peterson, Walter N. Moss, David H. Mathews, Elzbieta Kierzek
Summary: This study proposes a secondary structure for segment 8 vRNA of A/California/04/2009 (H1N1) for the first time and highlights the location of conserved structural motifs within IAV strains. The research has implications for understanding the functionality of viral RNA structures and developing inhibition methods against influenza A virus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Dev Kumar Verma, Neeraj Sood, Anutosh Paria, T. R. Swaminathan, C. V. Mohan, K. V. Rajendran, P. K. Pradhan
Summary: The tilapia lake virus (TiLV), a highly infectious viral pathogen, poses a major threat to the global tilapia industry. This study investigated the genomic evolution of TiLV and found that reassortment, selection, and mutation play important roles in its evolution. The results highlight the dominant force of reassortment in the emergence of this highly infectious segmented RNA virus.
Article
Microbiology
Julianna Han, Ketaki Ganti, Veeresh Kumar Sali, Carly Twigg, Yifeng Zhang, Senthamizharasi Manivasagam, Chieh-Yu Liang, Olivia A. Vogel, Iris Huang, Shanan N. Emmanuel, Jesse Plung, Lillianna Radoshevich, Jasmine T. Perez, Anice C. Lowen, Balaji Manicassamy
Summary: It is well documented that influenza A viruses selectively package 8 distinct viral ribonucleoprotein complexes (vRNPs) into each virion, but the exact role of host factors in genome assembly is not fully understood. This study shows that Rab11a+ vesicles play a crucial role in the congregation and assembly of vRNPs, enabling specific genome assembly and production of infectious virus particles. However, in the absence of Rab11a, viral RNA segments fail to congregate properly, resulting in defects in virus assembly and increased production of non-infectious particles.
Article
Virology
Timothy W. Thoner, Madeline M. Meloy, Jacob M. Long, Julia R. Diller, James C. Slaughter, Kristen M. Ogden
Summary: Reassortment, or genome segment exchange between viruses, is an important mechanism for generating novel virus genotypes and pandemic strains. This study investigates the factors influencing reassortment in reovirus, a segmented RNA virus, and finds that infection multiplicity and transcript abundance play key roles, whereas compartmentalization of replication and superinfection exclusion have minimal impact.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Tetsuya Yoshida, Masayuki Ishikawa, Kazuhiro Ishibashi
Summary: This study demonstrates that small RNA fragments derived from the NR region can inhibit the replication of TMV and affect the translation of mRNA.
Article
Agriculture, Multidisciplinary
Wang Yu-long, Fan Lin-jin, Jiang Nan, Gao Li, Li Kai, Gao Yu-long, Liu Chang-jun, Cui Hong-yu, Pan Qing, Zhang Yan-ping, Wang Xiao-mei, Qi Xiao-le
Summary: This study proposed an improved classification scheme for IBDV genotypes based on the molecular characteristics of VP2 and VP1, allowing for more accurate classification and identification of IBDV strains.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2021)
Article
Entomology
Eric Dubois, Marine Dardouri, Frank Schurr, Nicolas Cougoule, Fabrice Sircoulomb, Richard Thiery
Review
Biochemistry & Molecular Biology
Pierre Hardouin, Adeline Goulet
BIOCHEMICAL SOCIETY TRANSACTIONS
(2020)
Review
Virology
Adeline Goulet, Silvia Spinelli, Jennifer Mahony, Christian Cambillau
Article
Microbiology
Marianne Coulon, Anne Dalmon, Gennaro Di Prisco, Alberto Prado, Florine Arban, Eric Dubois, Magali Ribiere-Chabert, Cedric Alaux, Richard Thiery, Yves Le Conte
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Virology
Silvia Spinelli, Denise Tremblay, Sylvain Moineau, Christian Cambillau, Adeline Goulet
Article
Biotechnology & Applied Microbiology
Katherine Lavelle, Adeline Goulet, Brian McDonnell, Silvia Spinelli, Douwe van Sinderen, Jennifer Mahony, Christian Cambillau
MICROBIAL BIOTECHNOLOGY
(2020)
Article
Virology
Cecile Philippe, Amel Chaib, Fety Jaomanjaka, Stephanie Cluzet, Aurelie Lagarde, Patricia Ballestra, Alain Decendit, Melina Petrel, Olivier Claisse, Adeline Goulet, Christian Cambillau, Claire Le Marrec
Article
Microbiology
Adeline Goulet, Christian Cambillau
Summary: This study utilized the AlphaFold2 algorithm to predict the structure and topology of adhesion devices of two lytic siphophages infecting wine bacteria, revealing their distinct composition, morphology, and potential different infection mechanisms. The results highlight the ability of AlphaFold2 to accurately predict protein structures and its potential in the study of phage structures and host-binding mechanisms.
Article
Parasitology
Mateus de Souza Ribeiro Mioni, Luan Cleber Henker, Wanderson Sirley Reis Teixeira, Marina Paula Lorenzett, Marcelo Bahia Labruna, Saulo Petinatti Pavarini, David Driemeier, Elodie Rousset, Karim Sidi-Boumedine, Richard Thiery, Jane Megid
Summary: This study investigated the presence of C. burnetii in aborted bovine fetuses in Brazil and found a certain positivity rate. Additionally, histopathological features similar to fetal neosporosis were observed in some samples. These findings further confirm the negligence of this disease in Brazil and raise questions about the potential role of the pathogen in reproductive disorders in national livestock.
Review
Biochemistry & Molecular Biology
Emeline Grellet, India L'Hote, Adeline Goulet, Isabelle Imbert
Summary: This article reviews the history of coronavirus replication and provides detailed information on the core replicative machinery of these viruses.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Virology
Adeline Goulet, Christian Cambillau, Alain Roussel, Isabelle Imbert
Summary: Hepatitis E virus (HEV) is a major cause of acute viral hepatitis in humans globally, and it is now a global public health concern. The recent advancement in protein structure prediction software allows accurate prediction of the composition and structure of HEV replicases, which is important for producing stable and active proteins and conducting studies on structure, function, and inhibition.
Article
Microbiology
Aurelie Babin, Frank Schurr, Marie-Pierre Riviere, Marie-Pierre Chauzat, Eric Dubois
Summary: This study developed three quantitative real-time PCR (qPCR) methods and validated their accuracy and specificity in various bee samples. These methods can be used for the diagnosis of nosemosis and for studying the eco-dynamics of parasites in bees.
EUROPEAN JOURNAL OF PROTISTOLOGY
(2022)
Article
Green & Sustainable Science & Technology
Simon Hodge, Oliver Schweiger, Alexandra-Maria Klein, Simon G. Potts, Cecilia Costa, Matthias Albrecht, Joachim R. de Miranda, Marika Mand, Pilar De la Rua, Maj Rundlof, Eleanor Attridge, Robin Dean, Philippe Bulet, Denis Michez, Robert J. Paxton, Aurelie Babin, Nicolas Cougoule, Marion Laurent, Anne-Claire Martel, Laurianne Paris, Marie-Pierre Riviere, Eric Dubois, Marie-Pierre Chauzat, Karim Arafah, Dalel Askri, Sebastien N. Voisin, Tomasz Kiljanek, Irene Bottero, Christophe Dominik, Giovanni Tamburini, Maria Helena Pereira-Peixoto, Dimitry Wintermantel, Tom D. Breeze, Elena Cini, Deepa Senapathi, Gennaro Di Prisco, Piotr Medrzycki, Steffen Hagenbucher, Anina Knauer, Janine M. Schwarz, Risto Raimets, Vicente Martinez-Lopez, Kjell Ivarsson, Chris Hartfield, Pamela Hunter, Mark J. E. Brown, Jane C. Stout
Summary: To understand the negative impacts on insects in agricultural landscapes, it is important to assess multiple stressors and their interactions across large geographical scales. The PoshBee project established a network of 128 study sites across Europe and collected measurements and samples related to various aspects of the bees' environment. This paper describes the development process, rationale, and outcomes of the project, highlighting the challenges and actions that may benefit future large-scale studies.
Review
Virology
Tristan Durand, Anne Bonjour-Dalmon, Eric Dubois
Summary: Over the past few decades, honey bees have been facing an increasing number of stressors, and the interactions between these stressors have been identified as a key factor in colony mortality. Understanding the interactions between honey bee viruses is crucial for bee health, as viruses rely on the immune responses of bees. This review summarizes the current knowledge on the antiviral immunity of honey bees and examines the interactions between specific pathogenic viruses and their hosts.
Article
Health Care Sciences & Services
Geraldine Rios, Caroline Lacoux, Vianney Leclercq, Anna Diamant, Kevin Lebrigand, Adele Lazuka, Emmanuel Soyeux, Sebastien Lacroix, Julien Fassy, Aurelie Couesnon, Richard Thiery, Bernard Mari, Christian Pradier, Rainer Waldmann, Pascal Barbry
Summary: This study implemented a nanopore RNA sequencing monitoring system in Nice to analyze SARS-CoV-2 variants in wastewater samples, revealing the diversity of sequences in different neighborhoods. Wastewater sequencing correctly identified lineages found in clinical samples, demonstrating its potential for tracking pathogen sequence diversity in current and future outbreaks.
LANCET REGIONAL HEALTH-EUROPE
(2021)