Article
Genetics & Heredity
Michelle Miron, Wenzhao Meng, Aaron M. Rosenfeld, Shirit Dvorkin, Maya Meimei Li Poon, Nora Lam, Brahma V. Kumar, Yoram Louzoun, Eline T. Luning Prak, Donna L. Farber
Summary: Human memory T cell repertoire exhibits both widespread clone persistence across subsets and sites, as well as clones that are more restricted. CD8(+)T cells show greater clonal expansion and distinct TRBV usage compared to CD4(+)T cell subsets, with extensive sharing of clones between different tissues observed. Tissue-specific biases in clonal similarity are also evident through edit distance analysis.
Article
Multidisciplinary Sciences
Catherine J. Reynolds, Corinna Pade, Joseph M. Gibbons, David K. Butler, Ashley D. Otter, Katia Menacho, Marianna Fontana, Angelique Smit, Jane E. Sackville-West, Teresa Cutino-Moguel, Mala K. Maini, Benjamin Chain, Mahdad Noursadeghi, Tim Brooks, Amanda Semper, Charlotte Manisty, Thomas A. Treibel, James C. Moon, Ana M. Valdes, Aine McKnight, Daniel M. Altmann, Rosemary Boyton
Summary: Vaccination with a single dose of BNT162b2 after prior infection with a heterologous variant substantially enhances neutralizing antibody responses against variants. In contrast, individuals without prior infection showed reduced immunity against variants after receiving a single vaccine dose.
Article
Biochemistry & Molecular Biology
Jennifer E. Rood, Aidan Maartens, Anna Hupalowska, Sarah A. Teichmann, Aviv Regev
Summary: Single-cell atlases have the potential to bridge the gap between genes, diseases, and therapies. By understanding disease mechanisms at the cellular and tissue levels, they can aid in disease diagnostics, drug target identification, and the development of new therapies.
Article
Microbiology
Joanne Kite, Tiffany Russell, Juliet Jones, Gillian Elliott
Summary: Although nectin1 is required for extracellular entry into the majority of human keratinocytes, it is dispensable for direct cell-to-cell transmission.
Article
Immunology
Heloise Flament, Matthieu Rouland, Lucie Beaudoin, Amine Toubal, Leo Bertrand, Samuel Lebourgeois, Camille Rousseau, Pauline Soulard, Zouriatou Gouda, Lucie Cagninacci, Antoine C. Monteiro, Margarita Hurtado-Nedelec, Sandrine Luce, Karine Bailly, Muriel Andrieu, Benjamin Saintpierre, Franck Letourneur, Youenn Jouan, Mustapha Si-Tahar, Thomas Baranek, Christophe Paget, Christian Boitard, Anais Vallet-Pichard, Jean-Francois Gautier, Nadine Ajzenberg, Benjamin Terrier, Frederic Pene, Jade Ghosn, Xavier Lescure, Yazdan Yazdanpanah, Benoit Visseaux, Diane Descamps, Jean-Francois Timsit, Renato C. Monteiro, Agnes Lehuen
Summary: Severe cases of COVID-19 are characterized by immune system dysfunction, particularly a significant reduction and high activation of mucosal-associated invariant T (MAIT) cells. These cells play a crucial role in disease severity and mortality, correlating with the activation of inflammatory cytokines and cytotoxic phenotype.
Article
Multidisciplinary Sciences
Carys A. Croft, Anna Thaller, Solenne Marie, Jean-Marc Doisne, Laura Surace, Rui Yang, Anne Puel, Jacinta Bustamante, Jean-Laurent Casanova, James P. Di Santo
Summary: The Notch signaling pathway plays a crucial role in the development of innate lymphoid cells (ILCs) from human ILC precursors. The study identifies a Notch->RORC->IL-23R pathway that regulates ILC differentiation. These findings may have implications for expanding functional ILC subsets in vitro and developing novel ILC therapies for human diseases.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Rui Dai, Xiaopei Huang, Yiping Yang
Summary: This study demonstrates the critical role of gamma delta T cells in promoting the CD8(+) T cell response to VV infection. Gamma delta T cells are able to present viral antigens and activate CD8(+) T cells, with MyD88 signaling playing a key role in this process. Understanding the regulation of adaptive T cell response by gamma delta T cells may provide insight for the development of more effective vaccine strategies.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Gastroenterology & Hepatology
Benjamin G. Wiggins, Laura J. Pallett, Xiaoyan Li, Scott P. Davies, Oliver E. Amin, Upkar S. Gill, Stephanie Kucykowicz, Arzoo M. Patel, Konstantinos Aliazis, Yuxin S. Liu, Gary M. Reynolds, Brian R. Davidson, Amir Gander, Tu Vinh Luong, Gideon M. Hirschfield, Patrick T. F. Kennedy, Yuehua Huang, Mala K. Maini, Zania Stamataki
Summary: This study found that high and intermediate CD69 expressions marked human hepatic CD4(+) T-RM and a novel functionally distinct recirculating population, both shaped by the liver microenvironment to achieve diverse immunosurveillance.
Article
Medicine, Research & Experimental
Simon Hirschberger, Gabriele Strauss, David Effinger, Xaver Marstaller, Alicia Ferstl, Martin B. Mueller, Tingting Wu, Max Huebner, Tim Rahmel, Hannah Mascolo, Nicole Exner, Julia Hess, Friedrich W. Kreth, Kristian Unger, Simone Kreth
Summary: Studies suggest that a very-low-carbohydrate diet significantly impacts human T-cell immunity, enhancing cellular responses and favoring mitochondrial oxidative metabolism. Therefore, dietary interventions may be a clinical tool for improving immunity.
EMBO MOLECULAR MEDICINE
(2021)
Article
Biology
Ningbo Wu, Dongping Chen, Hongxiang Sun, Jianmei Tan, Yao Zhang, Tianyu Zhang, Yuheng Han, Hongzhi Liu, Xinxing Ouyang, Xiao-Dong Yang, Xiaoyin Niu, Jie Zhong, Zhengting Wang, Bing Su
Summary: The study reveals a novel regulatory role of MAP3K2 in intestinal T cell immunity via the IL-18-MAP3K2-JNK axis, providing a potential target for intervention in T cell-mediated colitis. MAP3K2-deficient T cells have reduced ability to induce colitis and affect Th1 cell differentiation in the intestine rather than Th17 cells.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Immunology
Marta Sanz, Brendan T. Mann, Paul L. Ryan, Alberto Bosque, Daniel J. Pennington, Holger Hackstein, Natalia Soriano-Sarabia
Summary: Under non-pathological conditions, human ?d T cells represent a small fraction of CD3(+) T cells in peripheral blood (1-10%). They constitute a unique subset of T lymphocytes that recognize stress ligands or non-peptide antigens through MHC-independent presentation. Major human ?d T cell subsets, Vd1 and Vd2, expand in response to microbial infection or malignancy, but possess distinct tissue localization, antigen recognition, and effector responses. By comparing highly purified Vd1 and Vd2 cells, this study found distinct genetic and phenotypic signatures that define functional differences in ?d T cell populations, suggesting that Vd1 and Vd2 T cells are different lineages. These findings will facilitate further investigation into the ligand specificity and unique role of Vd1 and Vd2 cells in early immune responses.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Medicine, Research & Experimental
Aleksei Titov, Regina Shaykhutdinova, Olga Shcherbakova, Yana Serdyuk, Savely A. Sheetikov, Ksenia Zornikova, Alexandra Maleeva, Alexandra Khmelevskaya, Dmitry Dianov, Naina T. Shakirova, Dmitry B. Malko, Maxim Shkurnikov, Stepan Nersisyan, Alexander Tonevitsky, Ekaterina Khamaganova, Anton Ershov, Elena Y. Osipova, Ruslan Nikolaev, Dmitry E. Pershin, Viktoria A. Vedmedskia, Michael Maschan, Victoria R. Ginanova, Grigory A. Efimov
Summary: This study evaluated T cell immune responses in vaccinated, convalescent, unexposed, and SARS-CoV-2-exposed individuals, identified multiple immunogenic epitopes, and discovered the presence of cross-reactive epitopes. The study also developed an in vitro diagnostic test using these epitopes, which showed high accuracy. For some asymptomatic seronegative individuals, a complete absence of T cell response to these immunogenic epitopes suggests a potential protective role of cross-reactive immune response.
Article
Multidisciplinary Sciences
Tom Snir, Hagit Philip, Miri Gordin, Alona Zilberberg, Sol Efroni
Summary: Immunotherapy plays a crucial role in cancer treatment, and the unique characteristics of an individual's T cell receptor repertoire are essential for its effectiveness. This study analyzed the repertoire through sequencing and generated a longitudinal dataset of T cell clones and their abundance after anti-CTLA-4 treatment in a mouse model for colorectal cancer. The dynamics of the repertoire over four weeks showed the ascending and descending clonal expansion of specific clones in response to treatment. The data can be used for treatment determination and prediction of its effect, as well as providing insights into the behavior of the immune system over time.
Review
Cell Biology
Hafid Ait-Oufella, Jean-Remi Lavillegrand, Alain Tedgui
Summary: Research shows that chronic inflammation triggered by cholesterol-rich lipoproteins is crucial in atherosclerosis development. Effector Th1 cells are proatherogenic, while regulatory T cells (Tregs) protect against atherosclerosis. Understanding how Tregs affect atherosclerosis could lead to new targeted therapies against cardiovascular disease.
Article
Biochemistry & Molecular Biology
Wei Zhang, Hae-Bin Park, Dhananjay Yadav, Juyoung Hwang, Eun-Koung An, Hee-Yun Eom, So-Jung Kim, Minseok Kwak, Peter Chang-Whan Lee, Jun-O Jin
Summary: The study revealed that Ecklonia cava fucoidan has the strongest impact on activating human dendritic cells, upregulating costimulatory molecules, major histocompatibility complex, and proinflammatory cytokines production. It also showed the most significant induction of T cell proliferation and IFN-gamma production compared to other fucoidans, suggesting it could be a suitable candidate for enhancing immune activation in humans.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Virology
Georgios Katzilieris-Petras, Xin Lai, Ahmad S. Rashidi, Georges M. G. M. Verjans, Line S. Reinert, Soren R. Paludan
Summary: This study demonstrates the vital role of microglia in controlling the replication of HSV1 in the central nervous system. Depletion of microglia leads to increased viral titers and disease severity, highlighting their importance in the early antiviral response. The study also reveals the contribution of the immune sensor cGAS in the activity of microglia.
JOURNAL OF VIROLOGY
(2022)
Article
Multidisciplinary Sciences
Charlotte A. James, Yuexin Xu, Melissa S. Aguilar, Lichen Jing, Erik D. Layton, Martine Gilleron, Adriaan J. Minnaard, Thomas J. Scriba, Cheryl L. Day, Edus H. Warren, David M. Koelle, Chetan Seshadri
Summary: This study reveals the importance of CD4 and CD8 co-receptors in recognizing mycobacterial glycolipid antigens. The expression of either CD4 or CD8 increases the avidity of T cell receptors for mycolipids, leading to a higher intensity of fluorescence and decreased secretion threshold for interferon-gamma. Furthermore, CD8+ T cells show greater cytotoxicity compared to CD4+ T cells in individuals with active tuberculosis.
NATURE COMMUNICATIONS
(2022)
Article
Medicine, Research & Experimental
Lichen Jing, Xia Wu, Maxwell P. Krist, Tien-Ying Hsiang, Victoria L. Campbell, Christopher L. McClurkan, Sydney M. Favors, Lawrence A. Hemingway, Charmie Godornes, Denise Q. Tong, Stacy Selke, Angela C. LeClair, Chu-Woo Pyo, Daniel E. Geraghty, Kerry J. Laing, Anna Wald, Michael Gale, David M. Koelle
Summary: SARS-CoV-2 elicits a strong T cell response, with CD8 and CD4 T cells from convalescent individuals recognizing infected cells and inactivated whole viral antigen respectively. CD8 T cells exhibit low specificity, while CD4 T cells show higher breadth. Additionally, some CD4 T cell lines enriched using SARS-CoV-2 can cross-recognize seasonal coronavirus antigens.
Article
Hematology
Christopher Pleyer, Kerry J. Laing, Mir A. Ali, Christopher L. McClurkan, Susan Soto, Inhye E. Ahn, Pia Nierman, Emeline Maddux, Jennifer Lotter, Jeanine Superata, Xin Tian, Adrian Wiestner, Jeffrey Cohen, David M. Koelle, Clare Sun
Summary: Vaccination responses in CLL patients vary depending on the treatment status, with untreated patients showing better antibody and cellular responses compared to those receiving BTKi therapy.
Article
Oncology
Evalyn E. A. P. Mulder, Jeffrey Damman, Danielle Verver, Astrid A. M. van der Veldt, Sam Tas, Tamana Khemai-Mehraban, Kim C. Heezen, Roxane A. Wouters, Cornelis Verhoef, Georges M. G. M. Verjans, Anton W. Langerak, Dirk J. Grunhagen, Antien L. Mooyaart
Summary: T-VEC treatment in melanoma patients leads to the presence of plasma cells in biopsy samples. An angiocentric granulomatous pattern is associated with a durable complete response. These findings suggest that plasma cells may play a role in the response mechanism.
Article
Immunology
Peter-Paul A. Unger, Anna E. Oja, Tamana Khemai-Mehraban, Werner J. D. Ouwendijk, Pleun Hombrink, Georges M. G. M. Verjans
Summary: This study revealed that the human trigeminal ganglia (TG) is an immunocompetent environment where both CD4 and CD8 tissue-resident memory T-cells (T-RM) are established and retained. These findings provide support for T-RM-targeted vaccine strategies.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Multidisciplinary Sciences
Kerry J. Laing, Werner J. D. Ouwendijk, Victoria L. Campbell, Christopher L. McClurkan, Shahin Mortazavi, Michael Elder Waters, Maxwell P. Krist, Richard Tu, Nhi Nguyen, Krithi Basu, Congrong Miao, D. Scott Schmid, Christine Johnston, Georges M. G. M. Verjans, David M. Koelle
Summary: This study reveals that varicella-zoster virus-specific T cells preferentially persist as tissue-resident-memory T cells in the rash-involved skin after recovery from zoster, indicating their importance in controlling skin infections.
NATURE COMMUNICATIONS
(2022)
Article
Hematology
Chaitra Ujjani, Ted A. Gooley, Stephen E. Spurgeon, Deborah M. Stephens, Catherine Lai, Catherine M. Broome, Susan O'Brien, Haiying Zhu, Kerry J. Laing, Allison M. Winter, Georgios Pongas, Alexander L. Greninger, David M. Koelle, Tanya Siddiqi, Matthew S. Davids, Kerry A. Rogers, Alexey V. Danilov, Amy Sperling, Brian Tu, Tyler Sorensen, Kelsey Launchbury, Carlissa J. Burrow, Genesis Quezada, Joshua A. Hill, Mazyar Shadman, Philip A. Thompson
Summary: This study aimed to analyze the immune responses of patients with chronic lymphocytic leukemia (CLL) to the COVID-19 vaccine, including T-cell responses, and the impact of CLL therapeutics. The study found that patients receiving B-cell-directed therapy were less likely to have immune conversion. Additionally, the immune response persisted at 6 months, and administration of booster vaccinations enhanced the immune response. However, caution should be exercised as newer variants emerge and escape vaccine-induced immunity.
Article
Immunology
Michiel van Gent, Werner J. D. Ouwendijk, Victoria L. Campbell, Kerry J. Laing, Georges M. G. M. Verjans, David M. Koelle
Summary: This study suggests that VZV-specific CD8 T-cells play a limited role in maintaining latent infection of varicella-zoster virus in human trigeminal ganglia.
JOURNAL OF NEUROINFLAMMATION
(2023)
Review
Virology
S. Reshwan K. Malahe, Jeroen J. A. van Kampen, Olivier C. C. Manintveld, Rogier A. S. Hoek, Caroline M. M. den Hoed, Carla C. C. Baan, Marcia M. L. Kho, Georges M. G. M. Verjans
Summary: Solid organ transplant recipients (SOTRs) face a high risk of human herpesvirus (HHV)-related morbidity and mortality due to immunosuppressive therapy. We aim to increase awareness of the HHV disease burden in SOTRs by providing an overview of current prevention and management strategies from the literature and guidelines. We discuss challenges in prevention, treatment, and future perspectives.
Article
Medicine, Research & Experimental
Daphne M. Hullegie-Peelen, Hector Tejeda Mora, Dennis A. Hesselink, Eric M. J. Bindels, Thierry P. P. van den Bosch, Marian C. Clahsen-van Groningen, Marjolein Dieterich, Sebastiaan Heidt, Robert C. Minnee, Georges M. G. M. Verjans, Martin J. Hoogduijn, Carla C. Baan
Summary: This study demonstrates the presence of diverse populations of tissue-resident lymphocytes (TRLs) in kidney transplants, including innate, innate-like, and adaptive TRLs. Chimerism of donor and recipient cells is common in kidney transplants and is present in all types of tissue-resident memory T cells. These cells may contribute to local protection against viral pathogens.
Article
Immunology
Emily S. Ford, Koshlan Mayer-Blackwell, Lichen Jing, Kerry J. Laing, Anton M. Sholukh, Russell St. Germain, Emily L. Bossard, Hong Xie, Thomas H. Pulliam, Saumya Jani, Stacy Selke, Carlissa J. Burrow, Christopher L. McClurkan, Anna Wald, Alexander L. Greninger, Michael R. Holbrook, Brett Eaton, Elizabeth Eudy, Michael Murphy, Elena Postnikova, Harlan S. Robins, Rebecca Elyanow, Rachel M. Gittelman, Matyas Ecsedi, Elise Wilcox, Aude G. Chapuis, Andrew Fiore-Gartland, David M. Koelle
Summary: Hybrid immunity, resulting from a combination of vaccination and previous infection, offers greater protection against SARS-CoV-2 than either vaccination or prior infection alone. This study investigated the dynamics of spike-reactive T cells in individuals with hybrid immunity, following either SARS-CoV-2 infection or mRNA vaccination. The findings show that vaccination leads to expansions of memory T cell clones, while also generating diverse new T cell clones. The study provides insights into T cell responses to vaccines and emerging pathogens.
Article
Biotechnology & Applied Microbiology
Jason Z. Zhang, Hsien-Wei Yeh, Alexandra C. Walls, Basile I. M. Wicky, Kaitlin R. Sprouse, Laura A. VanBlargan, Rebecca Treger, Alfredo Quijano-Rubio, Minh N. Pham, John C. Kraft, Ian C. Haydon, Wei Yang, Michelle DeWitt, John E. Bowen, Cameron M. Chow, Lauren Carter, Rashmi Ravichandran, Mark H. Wener, Lance Stewart, David Veesler, Michael S. Diamond, Alexander L. Greninger, David M. Koelle, David Baker
Summary: The protein biosensor designed in this study utilizes thermodynamic coupling to detect neutralizing antibodies against SARS-CoV-2 variants, without the need for target modification, and can better distinguish differences between samples compared to traditional methods.
NATURE BIOTECHNOLOGY
(2022)
Meeting Abstract
Medicine, General & Internal
S. Wanner, V. L. Campbell, S. Selke, D. M. Koelle
JOURNAL OF INVESTIGATIVE MEDICINE
(2022)
