Article
Biochemistry & Molecular Biology
Sebastian Blatt, Daniel G. E. Thiem, Andreas Pabst, Bilal Al-Nawas, Peer W. Kaemmerer
Summary: The combination of bone substitute materials (BSMs) with platelet-rich fibrin (PRF) showed significant positive pro-angiogenic effects, particularly with alloplastic and xenogeneic materials, leading to increased vessel formation in vivo. This demonstrates the enhanced angiogenetic potential of BSMs when combined with PRF, suggesting potential for bony regeneration in clinical applications.
Review
Biotechnology & Applied Microbiology
Shuang Liang, Zhiqiang Zheng, Yaxin Li, Yuanming Yang, Lifeng Qin, Zhen Zhao, Licun Wang, Haiyan Wang
Summary: This review summarizes the clinical approaches and advantages of autologous platelet-rich plasma (PRP) in the management of enteric fistula (EF). The mechanism of action of PRP in EF treatment remains unclear, but it may promote healing through the involvement of cytokines.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Medicine, Research & Experimental
Roberta Sessa Stilhano, Priscila Martins Andrade Denapoli, Camila Congentino Gallo, Vivian Yochiko Samoto, Sheila Jean McNeill Ingham, Rene Jorge Abdalla, Timothy Jon Koh, Sang Won Han
Summary: This study aimed to investigate the effects of PPRP and LPRP on recovery from limb ischemia and growth factor expression. The results showed that both PRPs improved muscle fiber size and strength, reduced fibrosis and adipocytes, and did not significantly affect regenerative activities in the ischemic limb. In cell cultures, both PRPs were able to recover cell metabolism affected by serum deprivation.
Article
Physiology
Xiaoyao Liu, Mingjing Yin, Ying Li, Jianqun Wang, Junlong Da, Zhongshuang Liu, Kai Zhang, Lixue Liu, Wenxuan Zhang, Peijun Wang, Han Jin, Bin Zhang
Summary: This study successfully improved the performance of L-PRF scaffold by using genipin as a crosslinker. The results showed that genipin-modified L-PRF had better biomechanical performance, slower biodegradation, good bioavailability, and sustained release of growth factors. In vitro and in vivo experiments demonstrated that 0.01% w/v and 0.1% w/v genipin-crosslinked L-PRF had good porous structure, significantly promoted cell proliferation, and enhanced bone regeneration.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Medicine, Research & Experimental
Lien-Hung Huang, Cheng-Shyuan Rau, Wen-Hong Zeng, Tsu-Hsiang Lu, Yi-Chan Wu, Yi-Hong Chiu, Kai-Ming Wu, Chia-Wen Tsai, Chia-Wei Lin, Chia-Jung Wu, Yi-Chang Chung, Ching-Hua Hsieh
Summary: This study developed a method to prepare platelet-rich plasma (PRP) using a cationic copolymer-coated polyurethane sponge. The PRP obtained using this method showed high concentrations of platelets and angiogenic growth factors, and exhibited beneficial effects on cell migration and angiogenesis.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Cell & Tissue Engineering
Alessia Parascandolo, Michele Francesco Di Tolla, Domenico Liguoro, Manuela Lecce, Saverio Misso, Fabiana Micieli, Maria Rosaria Ambrosio, Serena Cabaro, Francesco Beguinot, Alessandra Pelagalli, Vittoria D'Esposito, Pietro Formisano
Summary: Platelet products are commonly used in regenerative medicine for wound healing, reduction of bleeding, tissue synthesis, and revascularization. Human platelet-rich plasma (hPRP) was found to support canine mesenchymal stem cells (cMSCs) by increasing their viability and migration. This study suggests that hPRP may be a promising tool for canine tissue regeneration and repair.
STEM CELLS INTERNATIONAL
(2023)
Review
Biochemistry & Molecular Biology
Peter A. Everts, Jose Fabio Lana, Kentaro Onishi, Don Buford, Jeffrey Peng, Ansar Mahmood, Lucas F. Fonseca, Andre van Zundert, Luga Podesta
Summary: Angiogenesis is critical for tissue repair, and autologous orthobiological cellular preparations, such as platelet-rich plasma (PRP), have been shown to improve tissue repair and regeneration. However, the PRP devices on the market differ in platelet numbers and bioformulation. The literature lacks specific qualifications for PRP preparation that can contribute to tissue repair and hardly addresses the impact of platelets and leukocytes on angiogenesis. This review explores the cellular composition of PRP and discusses the importance of platelet dosing and bioformulation strategies in initiating angiogenic pathways.
Letter
Surgery
Rosita Pensato, Rasmieh Al-Amer, Simone La Padula
Summary: The article presents a protocol for obtaining platelet-rich plasma (PRP), platelet-poor plasma (PPP), and thrombin for autologous use. It raises concerns about the lack of specific methodologies in PRP preparation and characterization, including platelet counts and leukocyte concentration. The use of non-activated platelets is suggested for better outcomes. Further analysis is needed to draw valid conclusions on PRP applications in aesthetic and regenerative medicine.
AESTHETIC PLASTIC SURGERY
(2023)
Article
Medicine, General & Internal
Jon Mercader Ruiz, Maider Beitia, Diego Delgado, Pello Sanchez, Jorge Guadilla, Cristina Perez de Arrilucea, Fernando Benito-Lopez, Lourdes Basabe-Desmonts, Mikel Sanchez
Summary: This study developed a new ultrafiltration technique for obtaining Platelet-Rich Plasma (PRP). The technique was able to concentrate growth factors and cytokines present in plasma, resulting in improved effectiveness compared to conventional PRPs.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Meral Nadra, Wanting Niu, Motoichi Kurisawa, Dominique Rousson, Myron Spector
Summary: In implant dentistry, the difficulties of reconstructing large defects in the oral cavity and musculoskeletal system using porous blocks have been addressed by developing a gelatin-based gel incorporating platelet-rich plasma lysate. This gel acts as a scaffold for cell migration and a vehicle for sustained release of therapeutic agents, promoting bone reconstruction.
BIOENGINEERING-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Jeffrey L. Van Eps, Joseph S. Fernandez-Moure, Fernando J. Cabrera, Francesca Taraballi, Francesca Paradiso, Silvia Minardi, Xin Wang, Bayan Aghdasi, Ennio Tasciotti, Bradley K. Weiner
Summary: The human bony skeleton undergoes constant remodeling with a turnover of up to 10% bone volume annually. The study evaluated the regenerative ability of a nanostructured MHA/Coll scaffold augmented by autologous PRP in a model of spinal fusion. Both groups showed significantly more new bone formation compared to controls, with Group 2 exhibiting enhanced bone production and successful fusion rates. This study demonstrates the potential of using nanostructured MHA/Coll scaffold and autologous PRP in achieving successful clinical fusion.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Medicine, General & Internal
Naoya Kikuchi, Tomokazu Yoshioka, Norihito Arai, Hisashi Sugaya, Kojiro Hyodo, Yu Taniguchi, Kosuke Okuno, Akihiro Kanamori, Masashi Yamazaki
Summary: This study retrospectively investigated the short-term effects of leukocyte-poor PRP (LP-PRP) treatment in 124 patients with knee osteoarthritis (KOA), showing a responder rate of 58.1%. Factors contributing to treatment efficacy included higher visual analog scale score before injection, white blood cell count in whole blood, and platelet concentration ratio of LP-PRP.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Polymer Science
Maryam Torabi, Mohammad Foad Abazari, Shohreh Zare Karizi, Mina Kohandani, Nazanin Hajati-Birgani, Sara Norouzi, Fatemeh Nejati, Alireza Mohajerani, Tahereh Rahmati, Zakiye Mokhames
Summary: In this study, PLGA and PRP were used to fabricate nanofibrous scaffolds, and it was found that iPSCs cultured on PLGA-PRP nanofibers were more likely to differentiate into cardiomyocytes compared to PLGA. Multidimensional evaluations at the morphological, molecular gene, and protein expression levels indicated the potential of PLGA-PRP in cardiac tissue engineering.
POLYMERS FOR ADVANCED TECHNOLOGIES
(2021)
Editorial Material
Orthopedics
Daniel J. Solomon
Summary: Multiple studies on platelet-rich plasma (PRP) in arthroscopic rotator cuff repair show mixed results, with recent meta-analysis highlighting the benefits of leukocyte-poor or pure PRP in reducing retear rate. Conflicting results in meta-analyses may stem from variations in study inclusion and exclusion criteria as well as search dates.
ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY
(2021)
Article
Dermatology
Ankita Tuknayat, Gurvinder Pal Thami, Mala Bhalla, Jasleen Kaur Sandhu
Summary: PRP treatment for melasma shows significant efficacy in reducing mMASI score, regardless of patients' skin type, gender, and type of melasma. Patient self-evaluation indicates high satisfaction with the results. No significant side effects were reported during the treatment.
DERMATOLOGIC THERAPY
(2021)
Article
Dentistry, Oral Surgery & Medicine
L. Pavez, N. Tobar, C. Chacon, R. Arancibia, C. Martinez, C. Tapia, A. Pastor, M. Gonzalez, J. Martinez, P. C. Smith
JOURNAL OF PERIODONTAL RESEARCH
(2018)
Article
Cell Biology
Claudia Oyanadel, Christopher Holmes, Evelyn Pardo, Claudio Retamal, Ronan Shaughnessy, Patricio Smith, Priscilla Cortes, Marcela Bravo-Zehnder, Claudia Metz, Teo Feuerhake, Diego Romero, Juan Carlos Roa, Viviana Montecinos, Andrea Soza, Alfonso Gonzalez
MOLECULAR BIOLOGY OF THE CELL
(2018)
Article
Dentistry, Oral Surgery & Medicine
Constanza E. Martinez, Roberto Gomez, Alexis M. Kalergis, Patricio C. Smith
CLINICAL ORAL INVESTIGATIONS
(2019)
Review
Physiology
Patricio C. Smith, Constanza Martinez, Jorge Martinez, Christopher A. McCulloch
FRONTIERS IN PHYSIOLOGY
(2019)
Article
Dentistry, Oral Surgery & Medicine
Jassir Paez, Romina Hernandez, Javier Espinoza, Leticia Rojas, Constanza E. Martinez, Nicolas Tobar, Jorge Martinez, Patricio C. Smith
JOURNAL OF PERIODONTAL RESEARCH
(2020)
Article
Dentistry, Oral Surgery & Medicine
Patricio C. Smith, Claudia Metz, Adely de la Pena, Claudia Oyanadel, Patricio Avila, Rodrigo Arancibia, Lucas Vicuna, Claudio Retamal, Francisca Barake, Alfonso Gonzalez, Andrea Soza
JOURNAL OF PERIODONTAL RESEARCH
(2020)
Article
Dentistry, Oral Surgery & Medicine
Ignacio Retamal, Romina Hernandez, Victoria Velarde, Alejandro Oyarzun, Constanza Martinez, Maria Julieta Gonzalez, Jorge Martinez, Patricio C. Smith
Article
Pharmacology & Pharmacy
Diana M. Alvarez, Luisa F. Duarte, Nicolas Corrales, Patricio C. Smith, Pablo A. Gonzalez
ANTIVIRAL RESEARCH
(2020)
Article
Microbiology
Estefania Castillo, Luisa F. Duarte, Nicolas Corrales, Diana M. Alvarez, Monica A. Farias, Adolfo Henriquez, Patricio C. Smith, Cristian Agurto-Munoz, Pablo A. Gonzalez
FRONTIERS IN MICROBIOLOGY
(2020)
Correction
Pharmacology & Pharmacy
Diana M. Alvarez, Luisa F. Duarte, Nicolas Corrales, Patricio C. Smith, Pablo A. Gonzalez
ANTIVIRAL RESEARCH
(2021)
Review
Cell Biology
Jorge Martinez, Patricio C. Smith
Summary: Desmoplastic tumors exhibit unique tissue structure with abnormal extracellular matrix deposition. Type I collagen is a major component linked to tumor cell survival and metastasis. Previous notion of desmoplasia as a reaction to tumor cells is challenged by findings of preexisting desmoplasia in breast tissue.
Article
Biochemistry & Molecular Biology
Ivan Ponce, Nelson Garrido, Nicolas Tobar, Francisco Melo, Patricio C. Smith, Jorge Martinez
Summary: The research revealed that increased tissue stiffness stimulates lactate production and glucose uptake by mammary fibroblasts, which is correlated with the expression of specific proteins. Additionally, mammary stromal cells cultured on stiff matrices generated factors that stimulated epithelial breast migration.
Article
Dentistry, Oral Surgery & Medicine
Fabio R. M. Leite, Gustavo G. Nascimento, Holger J. Moller, Georgios N. Belibasakis, Nagihan Bostanci, Patricio C. Smith, Rodrigo Lopez
Summary: The study found that fast gingivitis development was associated with higher levels of non-organized response and lower levels of organized response, while slow responders were not associated with gingivitis. The results suggest that individual variability may play a role in susceptibility to gingivitis.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2022)
Editorial Material
Immunology
Lesley Ann Bergmeier, Nicolas Dutzan, Patricio C. Smith, Heleen Kraan
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Susana Rios, Simon Alvarez, Patricio C. Smith, Claudia G. Saez, Catherine Andrade, Nelson Pinto, Constanza E. Martinez
Summary: This study analyzed the release of biomolecules from leucocyte- and platelet-rich fibrin (L-PRF) derived from smokers and nonsmokers, and investigated their effects on cell proliferation and migration in periodontal ligament cells. The results showed that L-PRF stimulated cell proliferation and migration, with no significant difference between smokers and nonsmokers. Nicotine inhibited cell proliferation and migration, but this effect was recovered in the presence of L-PRF conditioned media.
JOURNAL OF ORAL PATHOLOGY & MEDICINE
(2023)