Article
Gastroenterology & Hepatology
Allison Agus, Damien Richard, Tiphanie Fais, Emilie Vazeille, Melissa Chervy, Virginie Bonnin, Guillaume Dalmasso, Jeremy Denizot, Elisabeth Billard, Richard Bonnet, Anthony Buisson, Nicolas Barnich, Julien Delmas
Summary: Crohn's disease is a chronic inflammatory disorder influenced by gut microbiota, genetics, and environment. Dysbiosis, including an increase in pathogenic bacteria like AIEC and a decrease in anti-inflammatory SCFAs, is common in CD patients. Targeting SCFAs with a GPR43 agonist shows potential for controlling gut inflammation in CD.
Article
Microbiology
Romana R. Gerner, Suzana Hossain, Artur Sargun, Kareem Siada, Grant J. Norton, Tengfei Zheng, Wilma Neumann, Sean-Paul Nuccio, Elizabeth M. Nolan, Manuela Raffatellu
Summary: Inflammatory bowel diseases (IBD) are characterized by chronic inflammation of the gastrointestinal tract and alterations to the gut microbiome. This study found that immunization targeting siderophores reduced the colonization and association of adherent-invasive Escherichia coli (AIEC) with the gut mucosa, leading to a reduction in colitis severity. The study also laid the foundation for developing monoclonal antibodies against siderophores as a potential therapeutic strategy.
Article
Gastroenterology & Hepatology
Anthony Buisson, Harry Sokol, Nassim Hammoudi, Stephane Nancey, Xavier Treton, Maria Nachury, Mathurin Fumery, Xavier Hebuterne, Michael Rodrigues, Jean-Pierre Hugot, Gilles Boschetti, Carmen Stefanescu, Pauline Wils, Philippe Seksik, Lionel Le Bourhis, Madeleine Bezault, Pierre Sauvanet, Bruno Pereira, Matthieu Allez, Nicolas Barnich
Summary: This study investigates the role of adherent and invasive Escherichia coli (AIEC) bacteria in Crohn's disease (CD) using a postoperative recurrence model. The results suggest that AIEC colonization is associated with postoperative ileal lesions and endoscopic recurrence in CD patients. Additionally, AIEC colonization is linked to a specific microbiota signature.
Article
Gastroenterology & Hepatology
Anthony Buisson, Emilie Vazeille, Mathurin Fumery, Benjamin Pariente, Stephane Nancey, Philippe Seksik, Laurent Peyrin-Biroulet, Matthieu Allez, Nathalie Ballet, Jerome Filippi, Clara Yzet, Maria Nachury, Gilles Boschetti, Elisabeth Billard, Anaelle Dubois, Stephanie Rodriguez, Caroline Chevarin, Marion Goutte, Gilles Bommelaer, Bruno Pereira, Xavier Hebuterne, Nicolas Barnich
Summary: The study identified a method to detect ileal colonization by AIEC in CD patients, where the abundance and invasive ability of ileal-associated total E. coli were significantly higher in AIEC-positive patients. The level of serum anti-total E. coli antibodies (AEcAb) was found to be a rapid and easier biomarker to identify patients with Crohn's disease harboring AIEC, with a high negative predictive value for AIEC positivity.
UNITED EUROPEAN GASTROENTEROLOGY JOURNAL
(2021)
Article
Microbiology
Maria Beatriz de Sousa Figueiredo, Elizabeth Pradel, Fanny George, Severine Mahieux, Isabelle Houcke, Muriel Pottier, Chantal Fradin, Christel Neut, Catherine Daniel, Antonino Bongiovanni, Benoit Foligne, Marie Titecat
Summary: The AIEC pathotype can lead to inflammatory bowel diseases, particularly Crohn's disease, by adhering to and invading intestinal epithelial cells. The genetic and phenotypic characteristics of AIEC isolates vary greatly based on clonality, host factors, and the gut microenvironment, making it essential to identify the determinants of AIEC pathogenicity.
Review
Gastroenterology & Hepatology
Sarah Mansour, Tahreem Asrar, Wael Elhenawy
Summary: The surge in inflammatory bowel diseases indicates the potential role of adherent-invasive Escherichia coli (AIEC) strains as disease modifiers. Understanding AIEC pathogenesis, including their metabolic versatility, evasion of host defense mechanisms, and promotion of inflammation, is crucial for developing antimicrobials that can selectively eradicate CD-associated bacteria.
Article
Biochemistry & Molecular Biology
Guillaume Dalmasso, Hang Thi Thu Nguyen, Tiphanie Fais, Sebastien Massier, Caroline Chevarin, Emilie Vazeille, Nicolas Barnich, Julien Delmas, Richard Bonnet
Summary: The study revealed that the yersiniabactin siderophore of AIEC provides an advantage for growth in competitive environments, but it also activates autophagy leading to bacterial clearance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Rika Tanaka, Jin Imai, Hitoshi Tsugawa, Karl Bil Eap, Masaki Yazawa, Motoki Kaneko, Masashi Ohno, Kohei Sugihara, Sho Kitamoto, Hiroko Nagao-Kitamoto, Nicolas Barnich, Masashi Matsushima, Takayoshi Suzuki, Tatehiro Kagawa, Yasuhiro Nishizaki, Hidekazu Suzuki, Nobuhiko Kamada, Katsuto Hozumi
Summary: This study found that specific IgA produced by host immunity can recognize and restrict adherent-invasive E. coli (AIEC), a pathogen associated with Crohn's disease, from invading the colonic epithelium. This finding contributes to our understanding of the host immune response to AIEC and provides new insights for the treatment of Crohn's disease.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Cell Biology
Monica Viladomiu, Manirath Khounlotham, Belgin Dogan, Svetlana F. Lima, Ahmed Elsaadi, Emre Cardakli, Jim G. Castellanos, Charles Ng, Jeremy Herzog, Alexi A. Schoenborn, Melissa Ellermann, Bo Liu, Shiying Zhang, Ajay S. Gulati, R. Balfour Sartor, Kenneth W. Simpson, Steven M. Lipkin, Randy S. Longman
Summary: Endoplasmic reticulum stress is associated with Crohn's disease, particularly in cases with functional deficiency in protein disulfide isomerase anterior gradient 2 (AGR2). AGR2 deficiency leads to ER stress and ileocolitis, and its expression correlates with mucosal Enterobactericeae abundance. Agr2 deletion results in expansion of adherent-invasive Escherichia coli (AIEC), triggering Th17 cell ileocolitis. Mechanistically, AIEC-induced epithelial cell ER stress promotes IL-23 production by CD103(+) dendritic cells, and IL-23R is essential for ileocolitis in Agr2(-/-) mice.
Article
Multidisciplinary Sciences
Dhrubajyoti Nag, Dustin Farr, Saumya Raychaudhuri, Jeffrey H. Withey
Summary: In this study, a zebrafish model for studying Adherent-invasive Escherichia coli (AIEC) infection was developed. The probiotic E. coli Nissle 1917 (EcN) showed efficacy against AIEC, reducing colonization, tissue damage, and pro-inflammatory responses in zebrafish. This highlights the potential therapeutic use of EcN against AIEC in a zebrafish model.
Article
Microbiology
Ashok R. Nayak, Pradip K. Singh, Jinlei Zhao, Montserrat Samso, Michael S. Donnenberg
Summary: Type 4 pili are important surface appendages on bacteria and archaea that can extend and retract with great force. This study presents the detailed structure of an enzyme that converts chemical energy to mechanical energy in a human pathogen, revealing a mechanism of action not previously seen in enzymes that power type 4 pilus extension.
Article
Microbiology
Fernanda Pace, Sara E. Rudolph, Ying Chen, Bin Bao, David L. Kaplan, Paula Watnick
Summary: Short-chain fatty acids (SCFAs) produced by the bacterial community in the intestine have immunosuppressive effects on intestinal tissue and can impact gene expression in adherent-invasive Escherichia coli. Propionate and butyrate have been shown to increase AIEC virulence, especially in the Caco-2 monolayer model. However, the anti-inflammatory effects of propionate and butyrate may limit this virulent impact in HIE models. Consideration should be given to the potential of SCFAs to increase the virulence of intestinal pathogens when used as anti-inflammatory agents.
MICROBIOLOGY SPECTRUM
(2021)
Article
Microbiology
Monica Viladomiu, Maeva L. Metz, Svetlana F. Lima, Wen-Bing Jin, Lance Chou, Jri Live Cell Bank, Chun-Jun Guo, Gretchen E. Diehl, Kenneth W. Simpson, Ellen J. Scherl, Randy S. Longman
Summary: This study revealed the association of AIEC encoding PduC with the microbiome of CD patients, driving AIEC-induced intestinal T cell inflammation. The inflammatory cascade requires the catalytic activity of PduC to generate propionate, synergizing with lipopolysaccharide to induce IL-1 beta by MNPs. Disrupting fucose availability can limit AIEC-induced propionate production and intestinal inflammation.
CELL HOST & MICROBE
(2021)
Article
Immunology
Mireia Lopez-Siles, Carla Camprubi-Font, Eva M. Gomez del Pulgar, Miriam Sabat Mir, David Busquets, Yolanda Sanz, Margarita Martinez-Medina
Summary: The study found that adherent-invasive Escherichia coli (AIEC) may be associated with both Crohn's disease (CD) and ulcerative colitis (UC), but not with colorectal cancer (CRC). Additionally, further research on E. coli strains in celiac disease (CeD) would be of interest.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Gastroenterology & Hepatology
Frank Lichert
Summary: In the past 20 years, research on Crohn's disease has been intensified, yet much remains unknown about the pathophysiology of the disease. A study by Buisson et al. investigated the possible role of adherent-invasive E. coli (AIEC) in the early development of the disease.
ZEITSCHRIFT FUR GASTROENTEROLOGIE
(2023)
Article
Gastroenterology & Hepatology
Mathurin Fumery, Philippe Seksik, Claire Auzolle, Nicolas Munoz-Bongrand, Jean-Marc Gornet, Gilles Boschetti, Eddy Cotte, Anthony Buisson, Anne Dubois, Benjamin Pariente, Philippe Zerbib, Najim Chafai, Carmen Stefanescu, Yves Panis, Philippe Marteau, Karine Pautrat, Charles Sabbagh, Jerome Filippi, Marc Chevrier, Pascal Houze, Xavier Jouven, Xavier Treton, Matthieu Allez
AMERICAN JOURNAL OF GASTROENTEROLOGY
(2017)
Article
Gastroenterology & Hepatology
Carolina Palmela, Caroline Chevarin, Zhilu Xu, Joana Torres, Gwladys Sevrin, Robert Hirten, Nicolas Barnich, Siew C. Ng, Jean-Frederic Colombel
Article
Gastroenterology & Hepatology
Anthony Buisson, Emilie Vazeille, Regine Minet-Quinard, Marion Goutte, Damien Bouvier, Felix Goutorbe, Bruno Pereira, Nicolas Barnich, Gilles Bommelaer
JOURNAL OF CLINICAL GASTROENTEROLOGY
(2018)
Article
Gastroenterology & Hepatology
Matthieu Galtier, Luisa De Sordi, Adeline Sivignon, Amelie de Vallee, Damien Maura, Christel Neut, Oumaira Rahmouni, Kristin Wannerberger, Arlette Darfeuille-Michaud, Pierre Desreumaux, Nicolas Barnich, Laurent Debarbieux
JOURNAL OF CROHNS & COLITIS
(2017)
Article
Multidisciplinary Sciences
Charlotte Cordonnier, Lucie Etienne-Mesmin, Jonathan Thevenot, Amandine Rougeron, Sandra Renier, Benoit Chassaing, Arlette Darfeuille-Michaud, Nicolas Barnich, Stephanie Blanquet-Diot, Valerie Livrelli
SCIENTIFIC REPORTS
(2017)
Article
Cell Biology
Florie Maillard, Emilie Vazeille, Pierre Sauvanet, Pascal Sirvent, Richard Bonnet, Lydie Combaret, Pierre Chausse, Caroline Chevarin, Yolanda Fernandez Otero, Geoffrey Delcros, Vivien Chavanelle, Nathalie Boisseau, Nicolas Barnich
Review
Biochemistry & Molecular Biology
Romain Villeger, Amelie Lopes, Guillaume Carrier, Julie Veziant, Elisabeth Billard, Nicolas Barnich, Johan Gagniere, Emilie Vazeille, Mathilde Bonnet
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Review
Cell Biology
Anais Larabi, Nicolas Barnich, Hang Thi Thu Nguyen
Article
Microbiology
Anais Larabi, Laurene Salesse, Charlotte Cordonnier, Lucie Etienne-Mesmin, Nicolas Barnich, Guillaume Dalmasso, Hang Thi Thu Nguyen
Article
Biochemistry & Molecular Biology
Nicolas Barnich, Michael Rodrigues, Pierre Sauvanet, Caroline Chevarin, Sylvain Denis, Olivier Le Goff, Danielle Faure-Imbert, Thierry Hanh, Christian F. Roques, Benoit Chassaing, Monique Alric
Summary: The study demonstrates that two French natural mineral waters (NMWs) exhibit anti-inflammatory effects in intestinal epithelial cells, with one of them effectively reducing intestinal inflammation, disease activity score, fecal lipocalin-2 levels, and pro-inflammatory cytokine release in induced-colitis mice, while also preventing alterations in mucosa-associated microbiota. These findings highlight the potential clinical efficiency of such NMWs in contributing to a lower state of inflammation in inflammatory bowel disease in humans.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nutrition & Dietetics
Marine Dupuit, Vivien Chavanelle, Benoit Chassaing, Fanny Perriere, Monique Etienne, Claire Plissonneau, Audrey Boscaro, Nicolas Barnich, Vincent Pialoux, Thierry Maugard, Florian Le Joubioux, Sebastien Peltier, Pascal Sirvent, Yolanda F. Otero, Nathalie Boisseau
Summary: The combination of high-intensity interval training (HIIT) and TOTUM-63 has shown to limit weight gain, improve glycemic control, increase colon mucosa microbiota diversity, and enhance the relative abundance of beneficial bacteria. These results suggest that this combination could be effective for managing obesity and prediabetes.
Article
Nutrition & Dietetics
Carole Groussard, Claire Plissonneau, Laurie Josset, Frederic Capel, Mathilde Mura, Etienne Gouraud, Guillaume Mairesse, Guillaume Chesneau, Nicolas Barnich, Vincent Pialoux, Nathalie Boisseau
Summary: The study found that high-intensity interval training (HIIT) and linseed oil (LO) supplementation have independent beneficial effects on the pro/antioxidant balance, and their combination promotes SOD activity upregulation in subcutaneous adipose tissue.
Article
Nutrition & Dietetics
Nathalie Boisseau, Nicolas Barnich, Christelle Koechlin-Ramonatxo
Summary: The human gut microbiota plays a crucial role in both health and disease, and its composition and activity are influenced by factors such as diet and physical activity. Studies have shown that gut bacteria are sensitive to modulation by physical activity and that sports performance can be enhanced through the manipulation of gut microbiota. This highlights the importance of the nutrition-microbiota-physical activity triad in optimizing treatments and improving overall health.
Article
Gastroenterology & Hepatology
Gwladys Sevrin, Sebastien Massier, Benoit Chassaing, Allison Agus, Julien Delmas, Jeremy Denizot, Elisabeth Billard, Nicolas Barnich
Article
Gastroenterology & Hepatology
Amandine Lashermes, Ludivine Boudieu, Julie Barbier, Benoit Sion, Agathe Gelot, Nicolas Barnich, Denis Ardid, Frederic Antonio Carvalho