Article
Biochemistry & Molecular Biology
Meiyu Piao, Sung Ho Lee, Myeong Ji Kim, Joong-Kook Choi, Chang-Yeol Yeo, Kwang Youl Lee
Summary: Cyclophilin A (CypA) promotes osteoblast differentiation by increasing the DNA binding affinity of Runx2, and Akt signaling is upstream of CypA. This study provides important insights into the molecular mechanisms of osteoblast differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Meiyu Piao, Sung Ho Lee, Yuankuan Li, Joong-Kook Choi, Chang-Yeol Yeo, Kwang Youl Lee
Summary: This study reveals the positive impact of CypE on osteoblast differentiation by enhancing the transcriptional activity of Runx2 through its PPIase activity. The involvement of the Akt signaling pathway in CypE's function in osteoblast differentiation is also identified.
Review
Biochemistry & Molecular Biology
Toshihisa Komori
Summary: Runx2 is a crucial transcription factor in bone development, playing a key role in the maturation and proliferation of chondrocytes and osteoblasts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Kyung-Ran Park, Yoon-Ju Kwon, Yun Hee Jeong, Jin Tae Hong, Hyung-Mun Yun
Summary: In this study, it was found that Thel extracted from Polyozellus multiplex can promote osteoblast differentiation and bone matrix mineralization. Thel also regulates the expression of RUNX2 and MMP13, suggesting its potential as a therapeutic agent for bone disorders.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Medicine, Research & Experimental
Wenqian Xia, Xiao Han, Lin Wang
Summary: This study reveals that ETS1 is involved in the inhibitory effect of chronic high glucose on osteoblast differentiation and proliferation, indicating its potential as a therapeutic target for diabetes-induced osteoporosis.
JOURNAL OF BIOMEDICAL RESEARCH
(2022)
Article
Cell Biology
Hyung-Mun Yun, Bomi Kim, Ji Eun Park, Kyung-Ran Park
Summary: In this study, the bioactive compound trifloroside (TriFs) isolated from Gentianae Scabrae Radix was found to promote osteogenic activity and mineral apposition in pre-osteoblast cells. TriFs stimulated the major osteogenic BMP2-Smad1/5/8-RUNX2 pathway and increased cell migration and adhesion. This suggests that TriFs has potential as a drug target for osteoblast-mediated bone diseases.
Article
Cell Biology
Zihao Chen, Yong Li, Bibo Tan, Fang Li, Qun Zhao, Liqiao Fan, Zhidong Zhang, Xuefeng Zhao, Yu Liu, Dong Wang
Summary: This study revealed that Lnc_ASNR is highly expressed in gastric cancer and promotes cell proliferation, migration, and invasion by regulating the miR-519e-5p/FGFR2 pathway. Lnc_ASNR functions as a ceRNA targeting miR-519e-5p, and its effects are closely related to epithelial-mesenchymal transition (EMT) in gastric cancer development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Alessandro Fantin, Carlotta Tacconi, Emanuela Villa, Elena Ceccacci, Laura Denti, Christiana Ruhrberg
Summary: This study found that KIT is crucial for transient definitive hematopoiesis in the fetal liver during mouse embryo development, but dispensable for the process of yolk sac endothelial-to-hematopoietic transition.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Thomas H. Shin, Evangelos Theodorou, Carl Holland, Rae'e Yamin, Cathleen L. Raggio, Philip F. Giampietro, David A. Sweetser
Summary: This study identified a previously unrecognized role of Tle4 in bone calcification and investigated its impact on osteoblast function. Loss of Tle4 was shown to adversely affect bone development, potentially through regulation of key osteoblastogenic genes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Daehwan Choi, Takenobu Ishii, Munetada Ishikawa, Tomohisa Ootake, Hirokazu Kamei, Kohei Nagai, Kenji Sueishi
Summary: This study investigated the impact of osteoblast vibration on cell cycle, differentiation, and aging. Primary maxilla osteoblasts were subjected to vibration at different frequencies, and the results showed that cell proliferation increased significantly at 30 and 300 Hz. Differentiation of osteoblasts was also enhanced at 30 Hz, while markers of aging were activated. Overall, providing optimal vibration to osteoblasts promotes cell cycle progression and differentiation, but also induces cell aging.
Article
Cell & Tissue Engineering
Zihao Zou, Ranran Dai, Nan Deng, Wei Su, Ping Liu
Summary: The study revealed that exosomal miR-1275 derived from prostate cancer cells promotes the proliferation and activity of osteoblasts through modulation of the SIRT2/Runx2 signaling pathway.
CELL TRANSPLANTATION
(2021)
Article
Cell Biology
Haijing Fu, Rui Chen, Yue Wang, Yang Xu, Chun Xia, Bing Zhang
Summary: The study showed that RCN1 transferred from osteoblasts to NSCLC cells partially through extracellular vesicles, enhancing proliferation and migration of NSCLC cells while offsetting the inhibitory effect. RCN1 depletion-induced endoplasmic reticulum stress, positively regulated by autophagy, contributed to the suppression of proliferation and migration in NSCLC cells.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Endocrinology & Metabolism
Anthony D. Bird, Emily R. Frost, Stefan Bagheri-Fam, Brittany M. Croft, Janelle M. Ryan, Liang Zhao, Peter Koopman, Vincent R. Harley
Summary: During sex determination in mice, FGFR2c is involved in testis development while FOXL2 and WNT4/RSPO1 pathways drive ovarian development. The role of FGFR2 in the ovary and its requirement in the testis after sex determination are not clear.
Article
Environmental Sciences
Xianghu Wang, Mingjun Sun, Zhikui Gao, Lihong Yin, Yuepu Pu, Yong Zhu, Xiaobin Wang, Ran Liu
Summary: This study focuses on the relationship between N-nitrosamines exposure and esophageal squamous cell carcinoma (ESCC), revealing the crucial role of lncRNA-UCA1 in ESCC progression. The downregulation of UCA1 promotes ESCC development through the regulation of the hnRNP F/FGFR2/PI3K-AKT axis, impacting tumorigenesis.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Sachin Chaugule, Jung-Min Kim, Yeon-Suk Yang, Klaus-Peter Knobeloch, Xi He, Jae-Hyuck Shim
Summary: The study reveals the crucial role of the deubiquitinating enzyme USP8 in promoting Wnt/beta-catenin signaling, which is essential for the differentiation of skeletal stem cells and osteogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Qing Jiang, Xin Qin, Carolina Andrea Yoshida, Hisato Komori, Kei Yamana, Shinsuke Ohba, Hironori Hojo, Brad St Croix, Viviane K. S. Kawata-Matsuura, Toshihisa Komori
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Review
Biochemistry & Molecular Biology
Yoshifumi Takahata, Tomohiko Murakami, Kenji Hata, Riko Nishimura
Summary: Recent studies have focused on elucidating the molecular aspects of osteoarthritis using a stress-induced mechanical load model in mice. Chondrocyte hypertrophy is found to be induced in articular cartilage, potentially serving as a target for early treatment of osteoarthritis. Inflammatory cytokines and matrix proteinases are expected to be important molecules for the management of the disease.
CURRENT MOLECULAR PHARMACOLOGY
(2021)
Letter
Biochemistry & Molecular Biology
Kazuhisa Akiba, Satoshi Narumi, Riko Nishimura, Yuko Kato-Fukui, Shuji Takada, Yukihiro Hasegawa, Maki Fukami
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2020)
Article
Biology
Koichiro Ono, Kenji Hata, Eriko Nakamura, Shota Ishihara, Sachi Kobayashi, Masako Nakanishi, Michiko Yoshida, Yoshifumi Takahata, Tomohiko Murakami, Seiichi Takenoshita, Toshihisa Komori, Riko Nishimura, Toshiyuki Yoneda
Summary: Endochondral bone formation relies on the transcription factor Dmrt2, which is induced by Sox9 to promote chondrocyte hypertrophy and interact with Runx2 to regulate gene expression. Lack of Dmrt2 may disrupt the sequential chondrocyte differentiation in endochondral bone formation.
COMMUNICATIONS BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Xin Qin, Qing Jiang, Hisato Komori, Chiharu Sakane, Ryo Fukuyama, Yuki Matsuo, Kosei Ito, Toshihiro Miyazaki, Toshihisa Komori
Summary: The study revealed the crucial role of Runx2 in the expression of major bone matrix protein genes and Tnfsf11 after commitment into osteoblasts in mice, and its deficiency may lead to dwarfism and reduced bone mass in mice.
JOURNAL OF BONE AND MINERAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Ryogo Katada, Junichi Tanaka, Koki Takamatsu, Kenji Hata, Rika Yasuhara, Shintaro Ohnuma, Ikuko Takakura, Riko Nishimura, Tatsuo Shirota, Kenji Mishima
Summary: Salivary gland hypofunction caused by radiation therapy or Sjogren syndrome can lead to various oral diseases and decrease in quality of life. Inducing salivary gland-like cells from transdifferentiated epithelial tissues provides a promising cell therapy strategy for treating dry mouth.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biology
Eriko Nakamura, Kenji Hata, Yoshifumi Takahata, Hiroshi Kurosaka, Makoto Abe, Takaya Abe, Miho Kihara, Toshihisa Komori, Sachi Kobayashi, Tomohiko Murakami, Toshihiro Inubushi, Takashi Yamashiro, Shiori Yamamoto, Haruhiko Akiyama, Makoto Kawaguchi, Nobuo Sakata, Riko Nishimura
Summary: The study revealed Zfhx4 as a crucial transcriptional partner of Osterix in endochondral ossification, showing physical and genetic interactions between the two. Zfhx4 deficiency led to inhibited calcification and reduced expression of matrix metallopeptidase 13 in cartilage, resembling impaired chondrogenesis in Osterix deficient mice. Additionally, Zfhx4 interacted with Runx2 upstream of Osterix in the transcriptional network.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biology
Yoshifumi Takahata, Hiromasa Hagino, Ayaka Kimura, Mitsuki Urushizaki, Sachi Kobayashi, Kanta Wakamori, Chika Fujiwara, Eriko Nakamura, Kayon Yu, Hiroshi Kiyonari, Kana Bando, Tomohiko Murakami, Toshihisa Komori, Kenji Hata, Riko Nishimura
Summary: Scientists identify Smoc1 and Smoc2 as novel targets for Runx2, playing important roles in bone formation. Double knockout mice for Smoc1 and Smoc2 show skeletal developmental abnormalities, indicating their involvement in intramembranous and endochondral bone formation. Their findings may lead to novel therapeutic strategies for various bone and cartilage diseases.
COMMUNICATIONS BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Yoshifumi Takahata, Hiromasa Hagino, Ayaka Kimura, Mitsuki Urushizaki, Shiori Yamamoto, Kanta Wakamori, Tomohiko Murakami, Kenji Hata, Riko Nishimura
Summary: This review article summarizes the research progress on the functions and regulatory mechanisms of lubricin and growth differentiation factor 5 (Gdf5) in osteoarthritis (OA), as well as their roles in maintaining articular cartilage homeostasis and the development of OA. Molecular targeting of Prg4 and Gdf5 is expected to provide new insights into the etiology, pathogenesis, and potential treatment of OA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Tomohiko Murakami, Yuri Nakaminami, Yoshifumi Takahata, Kenji Hata, Riko Nishimura
Summary: Inflammation plays a crucial role in the pathogenesis of bone and joint diseases, with proinflammatory cytokines accelerating bone resorption and cartilage destruction. IL-1, an important inflammatory cytokine, significantly affects the development of bone and joint diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Yoshiki Omatsu, Shota Aiba, Tomonori Maeta, Kei Higaki, Kazunari Aoki, Hitomi Watanabe, Gen Kondoh, Riko Nishimura, Shu Takeda, Ung-il Chung, Takashi Nagasawa
Summary: The transcription factors Runx1 and Runx2 play critical roles in the cellular niches of hematopoietic stem cells (HSCs). Lack of these transcription factors in CAR cells is associated with increased fibrosis and reduced HSCs in bone marrow. Runx1 is predominantly expressed in CAR cells and is induced by the transcription factor Foxc1, which decreases fibrotic gene expression in CAR cells.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Shota Ishihara, Kenji Hata, Katsutoshi Hirose, Tatsuo Okui, Satoru Toyosawa, Narikazu Uzawa, Riko Nishimura, Toshiyuki Yoneda
Summary: The study reveals the involvement of GPR81 in regulating the malignant phenotype of breast cancer cells through metabolic reprogramming. Knockdown of GPR81 impairs glycolysis and lactate-dependent ATP production, leading to suppression of cell migration and invasion.
SCIENTIFIC REPORTS
(2022)
Article
Endocrinology & Metabolism
Hiroko Yagi, Yoshifumi Takahata, Tomohiko Murakami, Yuri Nakaminami, Hiromasa Hagino, Shiori Yamamoto, Shinya Murakami, Kenji Hata, Riko Nishimura
Summary: This study found that BMP2 regulates FRZB expression through Osterix and Msx2, which is of great significance for the treatment of osteoarthritis.
JOURNAL OF BONE AND MINERAL METABOLISM
(2022)
Article
Genetics & Heredity
Toshihiro Inubushi, Yuichiro Nakanishi, Makoto Abeid, Yoshifumi Takahata, Riko Nishimura, Hiroshi Kurosaka, Fumitoshi Irie, Takashi Yamashiro, Yu Yamaguchi
Summary: HA degradation mediated by TMEM2 plays a crucial role in neural crest development and the morphogenesis of neural crest derivatives.
Article
Biochemistry & Molecular Biology
Tomohiko Murakami, Yoshifumi Takahata, Kenji Hata, Kosuke Ebina, Katsutoshi Hirose, Lerdluck Ruengsinpinya, Yuri Nakaminami, Yuki Etani, Sachi Kobayashi, Takashi Maruyama, Hiroyasu Nakano, Takehito Kaneko, Satoru Toyosawa, Hiroshi Asahara, Riko Nishimura
Summary: Proinflammatory cytokine Sema4D plays a critical role in the pathogenesis of joint diseases by directly promoting cartilage destruction. Sema4D induces a proinflammatory response in articular chondrocytes by activating proteolytic enzymes that degrade cartilage. This induction is independent of RhoA but relies on NF-kappa B signaling and Ras-MEK-Erk1/2 signaling mediated by Plexin-B2 and c-Met receptors.