CRISPR-Cas9 cleavage efficiency correlates strongly with target-sgRNA folding stability: from physical mechanism to off-target assessment
出版年份 2017 全文链接
标题
CRISPR-Cas9 cleavage efficiency correlates strongly with target-sgRNA folding stability: from physical mechanism to off-target assessment
作者
关键词
-
出版物
Scientific Reports
Volume 7, Issue 1, Pages -
出版商
Springer Nature
发表日期
2017-03-06
DOI
10.1038/s41598-017-00180-1
参考文献
相关参考文献
注意:仅列出部分参考文献,下载原文获取全部文献信息。- CRISPR/Cas9 for Human Genome Engineering and Disease Research
- (2016) Xin Xiong et al. Annual Review of Genomics and Human Genetics
- Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
- (2016) John G Doench et al. NATURE BIOTECHNOLOGY
- Applications of CRISPR technologies in research and beyond
- (2016) Rodolphe Barrangou et al. NATURE BIOTECHNOLOGY
- Defining and improving the genome-wide specificities of CRISPR–Cas9 nucleases
- (2016) Shengdar Q. Tsai et al. NATURE REVIEWS GENETICS
- Different Effects of sgRNA Length on CRISPR-mediated Gene Knockout Efficiency
- (2016) Jian-Ping Zhang et al. Scientific Reports
- Targeting cellular mRNAs translation by CRISPR-Cas9
- (2016) Yuchen Liu et al. Scientific Reports
- Engineered CRISPR-Cas9 nucleases with altered PAM specificities
- (2015) Benjamin P. Kleinstiver et al. NATURE
- High-throughput functional genomics using CRISPR–Cas9
- (2015) Ophir Shalem et al. NATURE REVIEWS GENETICS
- Structure and specificity of the RNA-guided endonuclease Cas9 during DNA interrogation, target binding and cleavage
- (2015) Eric A. Josephs et al. NUCLEIC ACIDS RESEARCH
- Cas9-chromatin binding information enables more accurate CRISPR off-target prediction
- (2015) Ritambhara Singh et al. NUCLEIC ACIDS RESEARCH
- Rationally engineered Cas9 nucleases with improved specificity
- (2015) I. M. Slaymaker et al. SCIENCE
- In vivo genome editing improves muscle function in a mouse model of Duchenne muscular dystrophy
- (2015) C. E. Nelson et al. SCIENCE
- CCTop: An Intuitive, Flexible and Reliable CRISPR/Cas9 Target Prediction Tool
- (2015) Manuel Stemmer et al. PLoS One
- Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA
- (2014) Hiroshi Nishimasu et al. CELL
- Development and Applications of CRISPR-Cas9 for Genome Engineering
- (2014) Patrick D. Hsu et al. CELL
- Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease
- (2014) Carolin Anders et al. NATURE
- Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells
- (2014) Xuebing Wu et al. NATURE BIOTECHNOLOGY
- Improving CRISPR-Cas nuclease specificity using truncated guide RNAs
- (2014) Yanfang Fu et al. NATURE BIOTECHNOLOGY
- Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease
- (2014) Cem Kuscu et al. NATURE BIOTECHNOLOGY
- Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation
- (2014) John G Doench et al. NATURE BIOTECHNOLOGY
- GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
- (2014) Shengdar Q Tsai et al. NATURE BIOTECHNOLOGY
- CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences
- (2014) Y. Lin et al. NUCLEIC ACIDS RESEARCH
- Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes
- (2014) Mark D. Szczelkun et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Prevention of muscular dystrophy in mice by CRISPR/Cas9-mediated editing of germline DNA
- (2014) C. Long et al. SCIENCE
- The new frontier of genome engineering with CRISPR-Cas9
- (2014) J. A. Doudna et al. SCIENCE
- Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
- (2013) Lei S. Qi et al. CELL
- DNA targeting specificity of RNA-guided Cas9 nucleases
- (2013) Patrick D Hsu et al. NATURE BIOTECHNOLOGY
- RNA-Guided Human Genome Engineering via Cas9
- (2013) P. Mali et al. SCIENCE
- Multiplex Genome Engineering Using CRISPR/Cas Systems
- (2013) L. Cong et al. SCIENCE
- Kinetic Mechanism of Conformational Switch between Bistable RNA Hairpins
- (2012) Xiaojun Xu et al. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Find Funding. Review Successful Grants.
Explore over 25,000 new funding opportunities and over 6,000,000 successful grants.
ExploreDiscover Peeref hubs
Discuss science. Find collaborators. Network.
Join a conversation