Article
Biochemistry & Molecular Biology
Rosaria Russo, Margherita Romeo, Tim Schulte, Martina Maritan, Luca Oberti, Maria Monica Barzago, Alberto Barbiroli, Carlo Pappone, Luigi Anastasia, Giovanni Palladini, Luisa Diomede, Stefano Ricagno
Summary: This study demonstrates the specific binding of Cu2+ to the variable domain of amyloidogenic H7 with a sub-micromolar affinity using microscale thermophoresis, isothermal calorimetry and thermal melting. The binding of copper ions is just one of the several biochemical traits contributing to light chain amyloidosis soluble toxicity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Georg J. Rottenaicher, Benedikt Weber, Florian Ruehrnoessl, Pamine Kazman, Ramona M. Absmeier, Manuel Hitzenberger, Martin Zacharias, Johannes Buchner
Summary: Specific mutations in the patient V-L domain can lead to protein destabilization and amyloid fibril formation, with the key disease-causing mutations located in the variable CDRs but impacting the dynamics of conserved core regions. These mutations do not necessarily propagate fibril formation by providing specific side chain interactions within the fibril structure, but rather destabilize the V-L domain in a way that increases the dynamics of framework regions, affecting the conformational changes needed for fibril core formation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Engineering, Environmental
Jiahui Zhu, Jia Wang, Ruonan Chen, Qiurun Feng, Xinhua Zhan
Summary: In this study, polystyrene nanoparticles with different sizes and functional groups were used to investigate their internalization process in wheat roots and cells. The results showed that the size and surface functional group of the nanoparticles play important roles in their internalization, with size being the primary factor. These findings provide important information for understanding the behavior of nanoparticles in plant tissues and assessing their potential risks to food safety and agricultural sustainability.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Chemistry, Medicinal
Nicholas L. Yan, Diogo Santos-Martins, Reji Nair, Alan Chu, Ian A. Wilson, Kristen A. Johnson, Stefano Forli, Gareth J. Morgan, H. Michael Petrassi, Jeffery W. Kelly
Summary: The study identified drugs that could stabilize immunoglobulin light chains, suppressing protein aggregation through enhanced affinity. Analysis revealed potential stabilizers, which could improve the stability of the light chains, with some showing significant enhancement in affinity.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Review
Pharmacology & Pharmacy
Samrat Mazumdar, Deepak Chitkara, Anupama Mittal
Summary: The beneficial or deleterious effects of nanomedicines are influenced by their complex interactions with intracellular pathways and their subcellular fate. Manipulating the physicochemical attributes of nanocarriers can direct nanoparticles towards specific sub-cellular organelles, affecting cellular internalization and enhancing intracellular uptake via specific endocytic pathways.
ACTA PHARMACEUTICA SINICA B
(2021)
Article
Biochemistry & Molecular Biology
Kamlesh M. Makwana, Matthew P. Sarnowski, Jiayuan Miao, Yu-Shan Lin, Juan R. Del Valle
Summary: The study introduces a minimalist approach to mimic the aggregation-prone modules within tau, showing efficacy in inhibiting tau fibril formation through a series of experiments.
ACS CHEMICAL NEUROSCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Xiaolong Zeng, Yufei Wang, Yun-Shuai Huang, Jianxiong Han, Wen Sun, Hans-Juergen Butt, Xing-Jie Liang, Si Wu
Summary: This study reports a new strategy to enhance cellular internalization and tumor penetration of PEGylated anticancer drugs using red-light irradiation.
Article
Cell Biology
Markus Mukenhirn, Francesco Muraca, Delia Bucher, Edgar Asberger, Elisa Cappio Barazzone, Elisabetta Ada Cavalcanti-Adam, Steeve Boulant
Summary: This study confirms that simultaneous deletion of CLCA/B leads to abnormal actin structures at the ventral plasma membrane, described for the first time as functional invadopodia. The formation of these invadopodia is influenced by the proteolytic activity of MMP14, and can be regulated by controlling the delivery of MMP14 to the plasma membrane.
Article
Biochemistry & Molecular Biology
Gregory M. Rosenberg, Kevin A. Murray, Lukasz Salwinski, Michael P. Hughes, Romany Abskharon, David S. Eisenberg
Summary: Proteins with low-complexity domains (LCDs) are prone to self-association and pathogenic mutations within these domains can lead to amyloid aggregation. A computational approach identified amyloidogenic mutations within LCDs, including some in previously unidentified proteins like TRK-fused gene protein (TFG). Mutations in TFG were shown to increase amyloid fibril formation, suggesting TFG as a new amyloid protein associated with peripheral neuropathies.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Review
Cell Biology
Maria Gonzalez-Santamarta, Corentin Bouvier, Manuel S. Rodriguez, Wendy Xolalpa
Summary: The cell uses posttranslational modifications (PTMs) to control the localization, association with partners, stability, and activity of protein targets, and one of the most dynamic proteins involved in PTMs is Ubiquitin (Ub). Ub and other Ubiquitin-like (UbL) proteins can rebuild the architecture of a protein in a few minutes, changing its properties efficiently. The formation and remodeling of Ub chains are regulated by conjugating and deconjugating enzymes, and occur in response to physiologic and pathologic stimuli.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Akkaranunt Supakijsilp, Jing He, Xubo Lin, Jian Ye
Summary: This study investigates the interaction between nanoparticles and the pulmonary surfactant monolayer, focusing on the effects of nanoparticle elasticity on translocation across the monolayer. Molecular dynamics simulations reveal that increasing stiffness enhances the penetrability of nanoparticles, while soft nanoparticles remain trapped within the monolayer. Active cellular uptake pathways exhibit longer penetration times, greater penetration distances, and more contacts compared to passive pathways, due to ligand-receptor interactions.
Article
Multidisciplinary Sciences
L. Nie, A. C. Nusantara, V. G. Damle, R. Sharmin, E. P. P. Evans, S. R. Hemelaar, K. J. van der Laan, R. Li, F. P. Perona Martinez, T. Vedelaar, M. Chipaux, R. Schirhagl
Summary: Relaxometry measurement inherited from diamond magnetometry can be used to detect free radicals in living cells with subcellular resolution. This quantum sensing technique based on defects in diamond allows nanoscale magnetic resonance measurements to detect metabolic activity in mitochondria.
Article
Oncology
Marta Banaszkiewicz, Agnieszka Olejnik, Anna Krzywonos-Zawadzka, Kornela Halucha, Iwona Bil-Lula
Summary: The study showed that simulated ischemia/reperfusion led to increased expression of ALC1 and decreased expression of VLC1 in ventricular cardiomyocytes, demonstrating a potential adaptive mechanism to altered conditions.
MOLECULAR MEDICINE REPORTS
(2021)
Review
Chemistry, Multidisciplinary
Dmitry Kurouski
Summary: The aggregation of misfolded proteins in amyloidogenic diseases is influenced by lipids, which can alter the aggregation rate, secondary structure, and morphology of protein oligomers and fibrils. Lipids also affect the toxicity of amyloid oligomers and fibrils. Furthermore, pathological changes in lipid composition may trigger protein aggregation and lead to the formation of highly toxic oligomers and fibrils. Polyunsaturated fatty acids have a significant impact on the aggregation properties of amyloidogenic proteins.
ACCOUNTS OF CHEMICAL RESEARCH
(2023)
Article
Cardiac & Cardiovascular Systems
Yanli Xia, Xiao-hua Zhang, Naohiro Yamaguchi, Martin Morad
Summary: Gene editing of cardiac ryanodine receptor in human-induced pluripotent stem cell derived cardiomyocytes reveals that mutations in Ca2+-binding residues affect Ca2+ release and lead to remodeling of excitation-contraction coupling pathways. Furthermore, spontaneous beating persists even in mutant cells without Ca2+-induced release signals.
CARDIOVASCULAR RESEARCH
(2023)
Article
Oncology
Sanjay Kumar, David Murray, Surendra Dasari, Paolo Milani, David Barnidge, Benjamin Madden, Taxiarchis Kourelis, Bonnie Arendt, Giampaolo Merlini, Marina Ramirez-Alvarado, Angela Dispenzieri
Article
Hematology
Surbhi Sidana, Nidhi Tandon, Morie A. Gertz, Angela Dispenzieri, Marina Ramirez-Alvarado, David L. Murray, Taxiarchis V. Kourelis, Francis K. Buadi, Prashant Kapoor, Wilson Gonsalves, Rahma Warsame, Martha Q. Lacy, Robert A. Kyle, S. Vincent Rajkumar, Shaji K. Kumar, Nelson Leung
BRITISH JOURNAL OF HAEMATOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Pinaki Misra, Luis M. Blancas-Mejia, Marina Ramirez-Alvarado
Article
Oncology
Surbhi Sidana, Daniel P. Larson, Patricia T. Greipp, Rong He, Ellen D. McPhail, Angela Dispenzieri, David L. Murray, Surendra Dasari, Stephen M. Ansell, Eli Muchtar, Wilson I. Gonsalves, Taxiarchis V. Kourelis, Marina Ramirez-Alvarado, Prashant Kapoor, S. Vincent Rajkumar, Martha Q. Lacy, Francis K. Buadi, Nelson Leung, Robert A. Kyle, Shaji K. Kumar, Rebecca L. King, Morie A. Gertz
Article
Oncology
Torri L. Jordan, Khansaa Maar, Keely R. Redhage, Pinaki Misra, Luis M. Blancas-Mejia, Christopher J. Dick, Jonathan S. Wall, Angela Williams, Allan B. Dietz, Andre J. van Wijnen, Yi Lin, Marina Ramirez-Alvarado
Article
Biochemistry & Molecular Biology
Jessica Dennis, Julia Sealock, Rebecca T. Levinson, Eric Farber-Eger, Jacob Franco, Sarah Fong, Peter Straub, Donald Hucks, Wen-Liang Song, MacRae F. Linton, Pierre Fontanillas, Sarah L. Elson, Douglas Ruderfer, Abdel Abdellaoui, Sandra Sanchez-Roige, Abraham A. Palmer, Dorret Boomsma, Nancy J. Cox, Guanhua Chen, Jonathan D. Mosley, Quinn S. Wells, Lea K. Davis
Summary: Genetic risk factors for major depressive disorder and loneliness act pleiotropically to increase coronary artery disease risk in females.
MOLECULAR PSYCHIATRY
(2021)
Article
Multidisciplinary Sciences
Jonathan D. Mosley, Rebecca T. Levinson, Eric Farber-Eger, Todd L. Edwards, Jacklyn N. Hellwege, Adriana M. Hung, Ayush Giri, Megan M. Shuey, Christian M. Shaffer, Mingjian Shi, Evan L. Brittain, Wendy K. Chung, Iftikhar J. Kullo, Adelaide M. Arruda-Olson, Gail P. Jarvik, Eric B. Larson, David R. Crosslin, Marc S. Williams, Ken M. Borthwick, Hakon Hakonarson, Joshua C. Denny, Thomas J. Wang, Charles M. Stein, Dan M. Roden, Quinn S. Wells
SCIENTIFIC REPORTS
(2020)
Article
Cardiac & Cardiovascular Systems
Hao Yu Chen, Benjamin J. Cairns, Aeron M. Small, Hannah A. Burr, Athithan Ambikkumar, Andreas Martinsson, Sebastien Theriault, Hans Markus Munter, Brian Steffen, Richard Zhang, Rebecca T. Levinson, Christian M. Shaffer, Jian Rong, Emily Sonestedt, Line Dufresne, Johan Ljungberg, Ulf Naslund, Bengt Johansson, Dilrini K. Ranatunga, Rachel A. Whitmer, Matthew J. Budoff, Albert Nguyen, Ramachandran S. Vasan, Martin G. Larson, William S. Harris, Scott M. Damrauer, Ken D. Stark, S. Matthijs Boekholdt, Nicholas J. Wareham, Philippe Pibarot, Benoit J. Arsenault, Patrick Mathieu, Vilmundur Gudnason, Christopher J. O'Donnell, Jerome I. Rotter, Michael Y. Tsai, Wendy S. Post, Robert Clarke, Stefan Soderberg, Yohan Bosse, Quinn S. Wells, J. Gustav Smith, Daniel J. Rader, Mark Lathrop, James C. Engert, George Thanassoulis
Article
Critical Care Medicine
Timothy E. Thayer, Rebecca T. Levinson, Shi Huang, Tufik Assad, Eric Farber-Eger, Quinn S. Wells, Jonathan D. Mosley, Evan L. Brittain
Summary: This study found a positive association between BMI and pulmonary arterial pressure, but not with markers of pulmonary vascular remodeling. Mendelian randomization analyses supported that higher BMI causes higher systolic pulmonary artery pressure. BMI is a modifier of pulmonary hypertension severity in both PAH and PVH, with involvement primarily in the pathogenesis of PVH.
Article
Genetics & Heredity
Sabrina L. Mitchell, Daniel A. Carranza Leon, Sandip Chaugai, Vivian K. Kawai, Rebecca T. Levinson, Wei-Qi Wei, C. Michael Stein
PHARMACOGENOMICS JOURNAL
(2020)
Review
Cardiac & Cardiovascular Systems
Ricardo O. Ramirez Flores, Jan D. Lanzer, Christian H. Holland, Florian Leuschner, Patrick Most, Jobst-Hendrik Schultz, Rebecca T. Levinson, Julio Saez-Rodriguez
Summary: The study integrated 16 public transcriptomic studies to derive a consensus transcriptional signature of human end-stage HF. Through machine learning approaches and meta-analysis, it revealed consistent molecular changes across all studies.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2021)
Article
Multidisciplinary Sciences
Rebecca T. Levinson, Nataraja Sarma Vaitinidin, Eric Farber-Eger, Dan M. Roden, Thomas A. Lasko, Quinn S. Wells, Jonathan D. Mosley
Summary: Heart failure has different subtypes, with HFpEF more common in older women and HFrEF more associated with cardiac ischemia. Comorbidity patterns were present before the diagnosis of HFpEF, suggesting that recognizing these patterns may help in identifying high-risk individuals.
SCIENTIFIC REPORTS
(2021)
Article
Cardiac & Cardiovascular Systems
Hao Yu Chen, Christian Dina, Aeron M. Small, Christian M. Shaffer, Rebecca T. Levinson, Anna Helgadottir, Romain Capoulade, Hans Markus Munter, Andreas Martinsson, Benjamin J. Cairns, Linea C. Trudso, Mary Hoekstra, Hannah A. Burr, Thomas W. Marsh, Scott M. Damrauer, Line Dufresne, Solena Le Scouarnec, David Messika-Zeitoun, Dilrini K. Ranatunga, Rachel A. Whitmer, Amelie Bonnefond, Garoar Sveinbjornsson, Ragnar Danielsen, David O. Arnar, Gudmundur Thorgeirsson, Unnur Thorsteinsdottir, Daniel F. Gudbjartsson, Hilma Holm, Jonas Ghouse, Morten Salling Olesen, Alex H. Christensen, Susan Mikkelsen, Rikke Louise Jacobsen, Joseph Dowsett, Ole Birger Vesterage Pedersen, Christian Erikstrup, Sisse R. Ostrowski, Christopher J. O'Donnell, Matthew J. Budoff, Vilmundur Gudnason, Wendy S. Post, Jerome Rotter, Mark Lathrop, Henning Bundgaard, Bengt Johansson, Johan Ljungberg, Ulf Naslund, Thierry Le Tourneau, J. Gustav Smith, Quinn S. Wells, Stefan Soderberg, Kari Stefansson, Jean-Jacques Schott, Daniel J. Rader, Robert Clarke, James C. Engert, George Thanassoulis
Summary: This study conducted a genome-wide meta-analysis and discovered 17 loci associated with calcific aortic stenosis. The integration of functional, expression, and cross-phenotype data revealed the mechanisms of this disease. Dyslipidemia, inflammation, calcification, and adiposity were identified as important factors in the etiology of calcific aortic stenosis.
EUROPEAN HEART JOURNAL
(2023)
Article
Medicine, General & Internal
Jan D. Lanzer, Alberto Valdeolivas, Mark Pepin, Hauke Hund, Johannes Backs, Norbert Frey, Hans-Christoph Friederich, Jobst-Hendrik Schultz, Julio Saez-Rodriguez, Rebecca T. Levinson
Summary: Using systems medicine approaches, we analyzed comorbidity profiles of heart failure patients and identified distinct profiles for HFpEF and HFrEF. This can improve diagnosis and treatment for HFpEF patients.
Article
Genetics & Heredity
Jia-Lu Qiao, Rebecca T. Levinson, Bowang Chen, Stefan T. Engelter, Philipp Erhart, Brady J. Gaynor, Patrick F. McArdle, Kristina Schlicht, Michael Krawczak, Martin Stenman, Arne G. Lindgren, John W. Cole, Caspar Grond-Ginsbach
Summary: In this study, a novel scatterplot-based method was explored for detecting copy number variation (CNV) in microarray samples. Compared to standard methods, this method showed higher detection accuracy.
FRONTIERS IN GENETICS
(2023)