Identification of potential drug targets for tuberous sclerosis complex by synthetic screens combining CRISPR-based knockouts with RNAi

Myc and mRNA capping

(2015) Sianadh Dunn et al.   Biochimica et Biophysica Acta-Gene Regulatory Mechanisms

Combining Genetic Perturbations and Proteomics to Examine Kinase-Phosphatase Networks in Drosophila Embryos

(2014) Richelle Sopko et al.   DEVELOPMENTAL CELL

Estradiol and mTORC2 cooperate to enhance prostaglandin biosynthesis and tumorigenesis in TSC2-deficient LAM cells

(2014) Chenggang Li et al.   JOURNAL OF EXPERIMENTAL MEDICINE

ZNF300P1 Encodes a lincRNA that regulates cell polarity and is epigenetically silenced in type II epithelial ovarian cancer

(2014) Brian Gloss et al.   Molecular Cancer

Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation

(2014) John G Doench et al.   NATURE BIOTECHNOLOGY

ProteomeXchange provides globally coordinated proteomics data submission and dissemination

(2014) Juan A Vizcaíno et al.   NATURE BIOTECHNOLOGY

Microhomology-based choice of Cas9 nuclease target sites

(2014) Sangsu Bae et al.   NATURE METHODS

RNAi screening comes of age: improved techniques and complementary approaches

(2014) Stephanie E. Mohr et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells

(2014) R. Bottcher et al.   NUCLEIC ACIDS RESEARCH

Highly Specific Contractions of a Single CAG/CTG Trinucleotide Repeat by TALEN in Yeast

(2014) Guy-Franck Richard et al.   PLoS One

Optimization of Genome Engineering Approaches with the CRISPR/Cas9 System

(2014) Kai Li et al.   PLoS One

Enhanced Specificity and Efficiency of the CRISPR/Cas9 System with Optimized sgRNA Parameters in Drosophila

(2014) Xingjie Ren et al.   Cell Reports

DNA copy number evolution in Drosophila cell lines

(2014) Hangnoh Lee et al.   GENOME BIOLOGY

A simplified and efficient germline-specific CRISPR/Cas9 system forDrosophilagenomic engineering

(2013) Zachary L Sebo et al.   FLY

Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease

(2013) Scott J. Gratz et al.   GENETICS

Highly Efficient Genome Modifications Mediated by CRISPR/Cas9 in Drosophila

(2013) Z. Yu et al.   GENETICS

Autophagy-Dependent Metabolic Reprogramming Sensitizes TSC2-Deficient Cells to the Antimetabolite 6-Aminonicotinamide

(2013) Andrey A. Parkhitko et al.   MOLECULAR CANCER RESEARCH

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

(2013) Prashant Mali et al.   NATURE BIOTECHNOLOGY

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

(2013) Vikram Pattanayak et al.   NATURE BIOTECHNOLOGY

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease

(2013) Wataru Fujii et al.   NUCLEIC ACIDS RESEARCH

Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system

(2013) Li-En Jao et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Optimized gene editing technology for Drosophila melanogaster using germ line-specific Cas9

(2013) X. Ren et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

(2013) Ophir Shalem et al.   SCIENCE

Genetic Screens in Human Cells Using the CRISPR-Cas9 System

(2013) Tim Wang et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Highly Efficient Targeted Mutagenesis of Drosophila with the CRISPR/Cas9 System

(2013) Andrew R. Bassett et al.   Cell Reports

Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9

(2013) A. R. Bassett et al.   Biology Open

TBC1D7 Is a Third Subunit of the TSC1-TSC2 Complex Upstream of mTORC1

(2012) Christian C. Dibble et al.   MOLECULAR CELL

Efficient TALEN-mediated gene knockout in livestock

(2012) D. F. Carlson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Use and Abuse of RNAi to Study Mammalian Gene Function

(2012) W. G. Kaelin   SCIENCE

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

The cyclin-dependent kinase PITSLRE/CDK11 is required for successful autophagy

(2011) Simon Wilkinson et al.   Autophagy

A Protein Complex Network of Drosophila melanogaster

(2011) K.G. Guruharsha et al.   CELL

Stringent Analysis of Gene Function and Protein-Protein Interactions Using Fluorescently Tagged Genes

(2011) R. A. Neumuller et al.   GENETICS

Genome-scale RNAi on living-cell microarrays identifies novel regulators of Drosophila melanogaster TORC1-S6K pathway signaling

(2011) R. A. Lindquist et al.   GENOME RESEARCH

Mapping of signaling networks through synthetic genetic interaction analysis by RNAi

(2011) Thomas Horn et al.   NATURE METHODS

Efficacy and Safety of Sirolimus in Lymphangioleiomyomatosis

(2011) Francis X. McCormack et al.   NEW ENGLAND JOURNAL OF MEDICINE

Inference of RhoGAP/GTPase regulation using single-cell morphological data from a combinatorial RNAi screen

(2010) O. Nir et al.   GENOME RESEARCH

Everolimus for Subependymal Giant-Cell Astrocytomas in Tuberous Sclerosis

(2010) Darcy A. Krueger et al.   NEW ENGLAND JOURNAL OF MEDICINE

Rapamycin-Insensitive Up-Regulation ofMMP2and Other Genes in Tuberous Sclerosis Complex 2–Deficient Lymphangioleiomyomatosis-Like Cells

(2009) Po-Shun Lee et al.   AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY

Direct Response to Notch Activation: Signaling Crosstalk and Incoherent Logic

(2009) A. Krejci et al.   Science Signaling

The TSC1–TSC2 complex: a molecular switchboard controlling cell growth

(2008) Jingxiang Huang et al.   BIOCHEMICAL JOURNAL

Sirolimus for Angiomyolipoma in Tuberous Sclerosis Complex or Lymphangioleiomyomatosis

(2008) John J. Bissler et al.   NEW ENGLAND JOURNAL OF MEDICINE