Review
Cell Biology
Emmanuel Martin, Magali Suzanne
Summary: This review discusses the role of the actin nucleation complex Arp2/3 in epithelial tissue, including its influence on cell-cell cooperation and tissue morphogenesis. It plays a significant role in regulating cell-cell adhesion, intracellular trafficking, and protrusion formation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Bojian Ding, Heidy Y. Narvaez-Ortiz, Yuvraj Singh, Glen M. Hocky, Saikat Chowdhury, Brad J. Nolen
Summary: Arp2/3 complex nucleates branched actin filaments and provides pushing forces for cellular processes. This study reveals the contacts between Arp2/3 complex and the mother actin filament, suggesting that actin filaments stimulate subunit flattening for complex activation. However, limited contact between the bottom half of the complex and the mother filament may explain why actin filaments are required but insufficient to trigger nucleation during WASP-mediated activation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Agriculture, Dairy & Animal Science
Meng-Hao Pan, Rui Xu, Yiqian Zhang, Lu Yin, Ruoyu Li, Dongxu Wen, Sihai Lu, Yan Gao, Xiaoe Zhao, Qiang Wei, Bin Han, Baohua Ma
Summary: Our study demonstrates that the Arp2/3 complex is essential for maintaining cytoskeletal dynamics and mitochondrial function during goat oocyte maturation. Disruption of the Arp2/3 complex leads to impaired F-actin assembly, spindle organization, ROS accumulation, and increased early apoptosis in goat oocytes.
Article
Multidisciplinary Sciences
Steven Z. Chou, Moon Chatterjee, Thomas D. Pollard
Summary: By analyzing the images collected by electron cryo-microscopy, we reconstructed the structure of actin filament branch junctions formed by fission yeast Arp2/3 complex. The formation of this structure requires a conformational change of the Arp2/3 complex and the acquisition of a new buried surface area to maintain the stability of the branch.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Carsten Schwan, Alexander E. Lang, Andreas Schlosser, Setsuko Fujita-Becker, Abdulatif AlHaj, Rasmus R. Schroeder, Jan Faix, Klaus Aktories, Hans Georg Mannherz
Summary: Researchers found that binary toxins produced by Clostridioides bacteria can ADP-ribosylate the Arp2/3 complex and its associated proteins, thereby inhibiting their activity. This discovery reveals the mechanism by which Clostridioides affects the cellular cytoskeleton.
Article
Genetics & Heredity
Virginia L. King, Nathan K. Leclair, Alyssa M. Coulter, Kenneth G. Campellone
Summary: The actin cytoskeleton plays an important role in vital cellular processes, with the study uncovering the involvement of WASP family members JMY and WHAMM in DNA damage-induced apoptosis. These proteins contribute to a p53-dependent cell death pathway by promoting mitochondrial permeability, caspase cleavage, and actin nucleation. Additionally, the study reveals changes in gene expression upon JMY loss, including upregulation of the G-protein RhoD which impacts cell survival.
Article
Biochemistry & Molecular Biology
Shashi Prakash Singh, Peggy Paschke, Luke Tweedy, Robert H. Insall
Summary: PKB and SGK kinases play crucial roles in pseudopodia formation and cell motility, particularly in actin polymerization and recruitment of the Arp2/3 complex by the Scar/WAVE complex.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Cell Biology
Shuting Zhang, Dimitrios Vavylonis
Summary: The nucleation of actin filament branches by the Arp2/3 complex involves activation through nucleation promotion factors (NPFs), recruitment of actin monomers, and binding of the complex to the side of actin filaments. Our molecular dynamics simulations using a coarse-grained model revealed stable configurations of the Arp2/3 complex with actin monomers and the activating VCA domain of the NPF Wiskott-Aldrich syndrome protein, supporting prior structural studies and providing insight into mechanisms proposed in previous studies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Artem I. Fokin, Roman N. Chuprov-Netochin, Alexander S. Malyshev, Stephane Romero, Marina N. Semenova, Leonid D. Konyushkin, Sergey V. Leonov, Victor V. Semenov, Alexis M. Gautreau
Summary: Several compounds structurally related to CK-666 were found to suppress Arp2/3 activation, and two of them demonstrated improved in vivo efficiency compared to CK-666.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Vassilis Papalazarou, Laura M. Machesky
Summary: The Arp2/3 complex coordinates the formation of branched actin networks, which are sensitive to mechanical forces and allow cells to generate pushing forces, influencing cellular processes.
CURRENT OPINION IN CELL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
William J. Spencer, Nicholas F. Schneider, Tylor R. Lewis, Carson M. Castillo, Nikolai P. Skiba, Vadim Y. Arshavsky
Summary: The photoreceptor outer segment is a cilium filled with flat-tened disc membranes for light capture. The addition of new discs at the base of outer segments is driven by Arp2/3-mediated actin polymerization initiated by the pentameric WAVE complex. Knockout of WASF3, a subunit of the WAVE complex, disrupts actin polymerization and leads to disorganized membrane lamellae instead of a proper outer segment.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Microbiology
Autumn Paluck, Jaspreet Osan, Lauren Hollingsworth, Sattya Narayan Talukdar, Ali Al Saegh, Masfique Mehedi
Summary: RSV is a major viral factor causing pneumonia in children worldwide. The virus interacts with the host actin cytoskeleton, particularly the ARP2/3 complex, to facilitate its replication and spread. Understanding the modulation of the cytoskeleton by RSV and its role in lung pathobiology may lead to novel intervention strategies.
Article
Multidisciplinary Sciences
Yanan Wang, Giovanni Chiappetta, Raphael Guerois, Yijun Liu, Stephane Romero, Daniel J. Boesch, Matthias Krause, Claire A. Dessalles, Avin Babataheri, Abdul I. Barakat, Baoyu Chen, Joelle Vinh, Anna Polesskaya, Alexis M. Gautreau
Summary: The RAC1-WAVE-Arp2/3 signaling pathway generates branched actin networks that power lamellipodium protrusion of migrating cells. PPP2R1A is a protein that associates with the WAVE complex subunit ABI1 when RAC1 is activated and branched actin generation is blocked. PPP2R1A interacts with the WAVE Shell Complex at the lamellipodial edge and is required for migration persistence and RAC1-dependent actin polymerization. Mutations in PPP2R1A impair WAVE Shell Complex binding and migration regulation, suggesting its essential role in cell migration.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Luyan Cao, Foad Ghasemi, Michael Way, Antoine Jegou, Guillaume Romet-Lemonne
Summary: Activation of the Arp2/3 complex by actin nucleation-promoting factors leads to the formation of branching actin filaments, while direct nucleation of linear actin filaments is induced by SPIN90-Arp2/3. Interestingly, the VCA motifs of certain proteins destabilize existing branches and linear filament ends. However, branch stabilizer cortactin and destabilizer GMF have similar effects on SPIN90-activated Arp2/3. These findings highlight the importance of understanding the similarities and differences between the two mechanisms in regulating actin cytoskeleton dynamics, as well as the differential responses of branched and linear filaments to aging and mechanical stress.
Article
Multidisciplinary Sciences
Li Wang, Jiali Yu, Zishuo Yu, Qianmin Wang, Wanjun Li, Yulei Ren, Zhenguo Chen, Shuang He, Yanhui Xu
Summary: BAF and PBAF are chromatin remodeling complexes that create nucleosome-depleted regions for transcription activation. This study provides structural insights into PBAF-mediated nucleosome association by reporting the structure of a 13-subunit human PBAF in complex with acetylated nucleosome in ADP-BeF3-bound state.
NATURE COMMUNICATIONS
(2022)
Editorial Material
Cell Biology
David J. Kast, Roberto Dominguez
Article
Biochemistry & Molecular Biology
David J. Kast, Allison L. Zajac, Erika L. F. Holzbaur, E. Michael Ostap, Roberto Dominguez
Meeting Abstract
Biophysics
Yadaiah Madasu, David J. Kast, Cristian Suarez, Marissa Cardwell, Juan J. Martinez, David R. Kovar, Roberto Dominguez
BIOPHYSICAL JOURNAL
(2013)
Meeting Abstract
Biophysics
David J. Kast, Yadaiah Madasu, Changsong Yang, Malgorzata Boczkowska, Tatyana Svitkina, Roberto Dominguez
BIOPHYSICAL JOURNAL
(2013)
Meeting Abstract
Biophysics
Bengi Turegun, David J. Kast, Roberto Dominguez
BIOPHYSICAL JOURNAL
(2013)
Meeting Abstract
Biophysics
Brett A. Colson, Matthew A. Mauseth, David J. Kast, L. Michel Espinoza-Fonseca, Osha Roopnarine, David D. Thomas
BIOPHYSICAL JOURNAL
(2014)
Article
Biochemistry & Molecular Biology
Andrea Disanza, Sara Bisi, Moritz Winterhoff, Francesca Milanesi, Dmitry S. Ushakov, David Kast, Paola Marighetti, Guillaume Romet-Lemonne, Hans-Michael Mueller, Walter Nickel, Joern Linkner, Davy Waterschoot, Christophe Ampe, Salvatore Cortellino, Andrea Palamidessi, Roberto Dominguez, Marie-France Carlier, Jan Faix, Giorgio Scita
Article
Biochemistry & Molecular Biology
Bengi Turegun, David J. Kast, Roberto Dominguez
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Biochemistry & Molecular Biology
David J. Kast, Changsong Yang, Andrea Disanza, Malgorzata Boczkowska, Yadaiah Madasu, Giorgio Scita, Tatyana Svitkina, Roberto Dominguez
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2014)
Article
Multidisciplinary Sciences
Yadaiah Madasu, Cristian Suarez, David J. Kast, David R. Kovar, Roberto Dominguez
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2013)
Article
Cell Biology
David J. Kast, Roberto Dominguez
MOLECULAR BIOLOGY OF THE CELL
(2019)
Article
Multidisciplinary Sciences
David J. Kast, Roberto Dominguez
NATURE COMMUNICATIONS
(2019)
Article
Biochemistry & Molecular Biology
V. A. Ismail, T. Naismith, D. J. Kast
Summary: Lipid droplets (LDs) are important organelles involved in cellular metabolism. Little is known about the molecular factors that regulate LD growth and degradation. This study shows that the autophagy-related protein DFCP1 is an NTPase that regulates LD metabolism by modulating free fatty acid metabolism, and its accumulation on LDs is regulated by a previously unknown NTPase domain.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
David J. Kast, Roberto Dominguez